23 research outputs found
Ableism, the dis/ability binary and beyond: Stage 1 (OA edition)
No full textFull edition for public use. This dataset is the basis for the first research stage of the qualitative study "Ableism, the dis/ability binary and beyond". The aim of the project is to create a feminist intersectional approach to ableism. For this purpose, a close collaboration with independent researchers who adress ableism in their advocacy, activism and artvisim is implemented throughout all stages of the research project. This dataset contains a) data collected through a systematic search in three academic databases that produced an overview of sources (n=1459) and b) additional data that was purposefully selected by the collaborators (n=116). Data are presented in two separate EXCEL documents, including information about author(s), year of publication, title, type of document or source, language and abstract or a note where there was no abstract available, as well as information about who of the collaborators chose which sources. Through a multiple-step process, explained by the flow-chart, the systematically collected data corpus was narrowed down to sources that focus on (especially gendered) ableism from an intersectional perspective. Collaborators chose 3 sources each from the purposefully collected data corpus that they determined as most relevant for understanding ableism from an intersectional perspective
A streamlined CRISPR/Cas9 approach for fast genome editing in Toxoplasma gondii and Besnoitia besnoiti
No full textToxoplasma gondii (T. gondii) and Besnoitia besnoiti (B. besnoiti) are closely related coccidian parasites belonging to the phylum Apicomplexa, which comprises many other important pathogens of humans and livestock. T. gondii is considered a model organism for studying the cell biology of Apicomplexa mainly due to the ease of propagation in diverse host cells and the availability of a wide range of genetic tools. Conversely, B. besnoiti in vitro culture systems currently exist only for the acute phase of infection, and genetic manipulation has proven much more challenging. In recent years, the targeted editing of chromosomal DNA by the programmable CRISPR-associated (Cas)9 enzyme has greatly improved the scope and accuracy of genetic manipulation in T. gondii and related parasites but is still lagging in B. besnoiti. The CRISPR/Cas9 technology enables the introduction of single point and insertion/deletion mutations, precise integration of in-frame epitope tags, and deletions of genes at reduced time and cost compared to previous methods. Current protocols for CRISPR-mediated genome editing in T. gondii rely on either constitutive or transient expression of Cas9 as well as target specific sgRNAs encoded separately or together on transfected plasmid vectors. Constitutively expressed Cas9 carries the risk of toxicity, whilst the transient approach is laborious and error-prone. Here we present a protocol for plasmid vector-independent genome-editing using chemically synthesized and modified sgRNAs. This protocol allows for rapid, efficient, and cost-effective generation of mutant cell lines of T. gondii and B. besnoiti
Structure of the reduced microsporidian proteasome bound by PI31-like peptides in dormant spores
No full textProteasomes play an essential role in the life cycle of intracellular pathogens with extracellular stages by ensuring proteostasis in environments with limited resources. In microsporidia, divergent parasites with extraordinarily streamlined genomes, the proteasome complexity and structure are unknown, which limits our understanding of how these unique pathogens adapt and compact essential eukaryotic complexes. We present cryo-electron microscopy structures of the microsporidian 20S and 26S proteasome isolated from dormant or germinated Vairimorpha necatrix spores. The discovery of PI31-like peptides, known to inhibit proteasome activity, bound simultaneously to all six active sites within the central cavity of the dormant spore proteasome, suggests reduced activity in the environmental stage. In contrast, the absence of the PI31-like peptides and the existence of 26S particles post-germination in the presence of ATP indicates that proteasomes are reactivated in nutrient-rich conditions. Structural and phylogenetic analyses reveal that microsporidian proteasomes have undergone extensive reductive evolution, lost at least two regulatory proteins, and compacted nearly every subunit. The highly derived structure of the microsporidian proteasome, and the minimized version of PI31 presented here, reinforce the feasibility of the development of specific inhibitors and provide insight into the unique evolution and biology of these medically and economically important pathogens
Tinjauan Teologis Integritas Elia: Studi I Raja-Raja 17:1-19:21
No full textIntegrity is important as long as someone is still alive. The importance of integrity is so important since the time of Elijah's ministry that was very necessary to bring change in the midst of Israel, which is to return to true worship. The changes brought by Elijah were only determined because Elijah had integrity. How about today, is integrity still relevant for believers? The author himself believes that integrity is still relevant for contemporary believers. The reason, because the success or failure of a person is determined by integrity. Integrity itself has the meaning of the integrity of a person, that means talking about one's own truth
Penerapan Statistical Quality Control Untuk Mengidentifikasi Tingkat Pengangguran Terbuka (TPT) Tahun 2017-2019 di Sumatera Utara
No full textUnemployment is one of the problems that often arise in the development process. Rising unemployment can be a barrier to development and potentially cause problems in both social and economic areas. North Sumatera is among the top 10 provinces in Indonesia with the highest unemployment rate. Therefore, the author wants to identify the unemployment rate in North Sumatera province using the Statistical Quality Control method. Statistical quality Control (Statistic) is a technique used to control and manage the process of both manufacturing and services through the use of statistical methods. With the results obtained from the R control map (Range) is 1.124 (BKA = 2.8943; BKB = 0.00), and for observations using the X-Bar control map at Get 4.562 (BKA = 5.71; BKB = 3.413). From the results of this analysis can be seen that some areas are not yet under control standards or are the region with the most unemployment in the province of North Sumatra.Pengangguran merupakan salah satu masalah yang sering muncul dalam proses pembangunan. Meningkatnya pengangguran bisa menjadi penghambat bagi jalannya pembangunan dan berpotensi menimbulkan masalah-masalah dalam bidang sosial maupun ekonomi. Sumatera Utara termasuk ke dalam 10 besar Provinsi di Indonesia dengan angka pengangguran tertinggi. Maka dari itu penulis ingin mengidentifikasi tingkat pengangguran di Provinsi Sumatera Utara dengan menggunakan metode Statistical Quality Control. Pengendalian kualitas statistik (Statistic Quality Control) adalah teknik yang digunakan untuk mengendalikan dan mengelola proses baik manufaktur maupun jasa melalui penggunaan metode statistik. Dengan hasil yang di peroleh dari peta kendali R(Range) adalah 1,124 (BKA = 2,8943; BKB = 0,00), dan untuk pengamatan menggunakan peta kendali X-Bar di peroleh 4,562 (BKA = 5,71; BKB = 3,413). Dari hasil analisis ini dapat diketahui bahwa beberapa daerah masih belum berada dalam standar pengendalian atau merupakan daerah dengan jumlah pengangguran terbanyak di Provinsi Sumatera Utara.60 HalamanKertas Karya Diplom
AlphaFold models and supporting data for the annotation of Vairimorpha necatrix
No full text<p>V_necatrix_alphafold.zip - Contains AlphaFold models and associated files for all V. necatrix proteins.</p><p>chimerax_annotater_plugin.zip - Contains the ChimeraX plugin we developed and used to annotate the V. necatrix proteome.</p><p>v_necatrix_annotation_data.zip - Contains all data used in the ChimeraX plugin to annotate the V. necatrix proteome.</p><p>V_necatrix_proteome.fasta - Protein fasta file containing all protein sequences</p><p>V_necatrix_haplotype[1-4].fasta - Nucleotide fasta file for each haplotype</p>
Phylogenetic Relationships in Pterodroma Petrels Are Obscured by Recent Secondary Contact and Hybridization
No full textThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Divergent evolutionary processes associated with colonization of offshore islands
No full textWe dedicate this article to Michel Pascal, our outstanding co-author, who died on 5 January 2013, and to Anne Brundle, who gave us access to much archaeological material and who died in 2012. We acknowledge receipt of a Marie Curie Intra European Fellowship (to N.M.), support from the Swiss National Science Foundation (projects 31003A-127377, 3100A0-112072 and 3100-126074) to L.E. and G.H., funding from SYNTHESYS2 made available by the European Community Research Infrastructure under FP7 (‘Synthesis of Systematic Resources’, 226506-CP-CSA-Infra) to S.B., a Wellcome Trust University award to K.M.D. (GR071037) and overarching funding from the Arts and Humanities Research Council (project grant 119396). We thank V. Bretille, A. Frantz, M. Fuster, N. Gould, J. Herman, E. Jones, S. Martínek, R. Marwick, J. Michaux, S. Montuire, J. Pauperio, C. Scott, C. Tougard, B. Walther and N. Wheale for field specimens, T. White for assistance with IMa runs, and A. Ritchie, L. Shepherd and A. Sheridan for archaeological advice. We are grateful to the following for museum and archaeological samples: J. Barrett (MacDonald Institute, University of Cambridge), A. Brundle (Orkney Museum), C. David (Guernsey Museum), A. Ervynck (Flemish Heritage Institute), L. Gordon (Smithsonian Institute), J. Herman (National Museums of Scotland), D. Lee (Orkney College), R. Sabin (British Museum - Natural History, London) and G. Veron (Muséum national d'histoire naturelle, Paris).Peer reviewe
Correction to: Second International Consensus Conference on lesions of uncertain malignant potential in the breast (B3 lesions) (Breast Cancer Research and Treatment, (2019), 174, 2, (279-296), 10.1007/s10549-018-05071-1)
No full textThe article Second International Consensus Conference on lesions of uncertain malignant potential in the breast (B3 lesions), written by Christoph J Rageth, Elizabeth AM O’Flynn, Katja Pinker, Rahel A Kubik-Huch, Alexander Mundinger, Thomas Decker, Christoph Tausch, Florian Dammann, Pascal A. Baltzer, Eva Maria Fallenberg, Maria P Foschini, Sophie Dellas, Michael Knauer, Caroline Malhaire, Martin Sonnenschein, Andreas Boos, Elisabeth Morris, Zsuzsanna Varga, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on November 30, 2018 without open access. With the author(s)’ decision to opt for Open Choice the copyright of the article changed on May 30, 2019 to © The Author(s) 2018 and the article is forthwith distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons.org/licen ses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made. The original article has been corrected
An experimental genetically attenuated live vaccine to prevent transmission of Toxoplasma gondii by cats
No full text© 2019, The Author(s). Almost any warm-blooded creature can be an intermediate host for Toxoplasma gondii. However, sexual reproduction of T. gondii occurs only in felids, wherein fertilisation of haploid macrogametes by haploid microgametes, results in diploid zygotes, around which a protective wall develops, forming unsporulated oocysts. Unsporulated oocysts are shed in the faeces of cats and meiosis gives rise to haploid sporozoites within the oocysts. These, now infectious, sporulated oocysts contaminate the environment as a source of infection for people and their livestock. RNA-Seq analysis of cat enteric stages of T. gondii uncovered genes expressed uniquely in microgametes and macrogametes. A CRISPR/Cas9 strategy was used to create a T. gondii strain that exhibits defective fertilisation, decreased fecundity and generates oocysts that fail to produce sporozoites. Inoculation of cats with this engineered parasite strain totally prevented oocyst excretion following infection with wild-type T. gondii, demonstrating that this mutant is an attenuated, live, transmission-blocking vaccine
