4,216 research outputs found
Police Unions, Industrial Strategies and Political Influence: Some Recent History
No full textABSTRACTThis article examines an area of Australian industrial relations that has received little attention in the academic literature on employment-- police unionism. The article addresses two main issues. Firstly, it provides an examination of the changing nature of police work and management practices. Secondly, it evaluates the distinctive character of strategies developed by police unions and the legitimacy of their political role. The overview concludes that in an environment where the majority of unions are struggling to maintain membership and develop strategies that deal effectively with increasingly strict legislative constraints, police unions have been remarkably successful.<br/
Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose
No full textFriehs K, Lafferty RM. Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose. Journal of Biotechnology. 1989;10(3-4):285-291.Genetic engineering methods were used to enhance the substrate spectrum of Alcaligenes eutrophus H16, a poly-[beta]-hydroxybutyric acid (PHB) producer. Using parts of the vector pMMB33 and a 2.5 kb DNA fragment of the Bacillus subtilis chromosome a plasmid was constructed bearing the gene for levanase, an enzyme able to hydrolyze various saccharides. After transfer of the levanase gene by triparental conjugation, the gene, controlled by its own Bacillus subtilis promoter, is expressed in Alcaligenes eutrophus H16 and enables the strain to hydrolyze sucrose. However, growth on sucrose is limited; i.e. the sucrose is not transported efficiently into the cell and/or the levanase is not secreted into the medium
Roles and mechanisms of parasitism in aquatic microbial communities
No full textNext Generation Sequencing technologies are increasingly revealing that microbial taxa likely to be parasites or symbionts are probably much more prevalent and diverse than previously thought. Every well studied free-living species has parasites; parasites themselves can be parasitized. As a rule of thumb, there is an estimated 4 parasitic species for any given host, and the better a host is studied the more parasites are known to infect it. Therefore, parasites and other symbionts should represent a very large number of species and may far outnumber those with 'free-living' lifestyles. Paradoxically, free-living hosts, which form the bulk of our knowledge of biology, may be a minority! Microbial parasites typically are characterized by their small size, short generation time, and high rates of reproduction, with simple life cycle occurring generally within a single host. They are diverse and ubiquitous in the environment, comprising viruses, prokaryotes and eukaryotes. This Frontiers Research Topic sought to provide a broad overview but concise, comprehensive, well referenced and up-to-date state of the art for everyone involved with microbial parasites in aquatic microbial ecology
Dalitz plot analyses of B-0 -> (D-DK+)-K-0 and B+-> (D)over-bar(-)D(0)K(+) decays
No full text© 2015, APS Journal
Rare Decays at LHCb
No full textWe review recent results from the LHCb experiment on studies of particle decays that are forbidden or rare in the Standard Model. The studies include searches for lepton flavour violating decays of the lepton and the and mesons, and of and meson decays that would be mediated by Majorana neutrinos. Results are also presented for the rare processes and , , transitions, and
Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis
No full textFriehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology. 1986;3(5-6):333-341.A DNA fragment from Bacillus subtilis strain Marburg coding for the synthesis of an enzyme catalyzing sucrose hydrolysis was cloned in Escherichia coli and detected using simple direct selection of transformants growing on sucrose. Three different clones were obtained each having a 2.5 kb EcoRI-Pstl fragment in common which was shown to be sufficient to mediate growth on sucrose. This fragment was not identical with known cloned gene fragments of B. subtilis coding for the sucrose hydrolyzing enzymes sucrase and levansucrase. It could be shown that the 2.5 kb fragment codes for a third sucrose hydrolyzing enzyme, namely for levanase. In the case of E. coli this enzyme was found to be mainly intracellular; however, a small quantity was also excreted into the periplasmic space
DIODE LASER STUDY OF THE FUNDAMENTAL OF
No full text W. J. Lafferty, A. G. Maki, and E. K. Plyler, J. Chem. Phys. 40, 224 (1964). A. W. Mantz, P. Connes, G. Guelachvili, and C. Amiot, J. Mol. Spectrosc. 54, 43 (1975).""Author Institution: Scientific Research Staff Ford Motor Company; Department of Physics, The University of MichiganWe have used current-tunable, thin-film PbTe diode lasers to obtain Doppler-limited resolution of the band of in the region 1565-1600 . Roughly 60\% of the interval was covered using different modes of 2 lasers, each mode giving a tuning range of 1-3 cm, Calibration was obtained with HO and NH, while the frequency scale was given by the fringes of a 4-cm Ge etalon, recorded simultaneously using a double beam technique. Near 1600 cm a set of strong regularly spaced lines stands out, which is the R branch of the ground state transition. This band as wel as the ``hot” bands due to the bending vibration indicate a linear structure for the molecule. Preliminary values in cm for the ground state are 1587.392, B^{\prime\prime} - 0.07557, and B - B. This B^{\prime\prime} value differs from that reported by Lafferty et al., but agrees with that by Mantz et al. The next 2 strongest series were interpretable as a band with = 1580.910, B = 0.07606, B - 0.07646, B - B = 4,178 X 10, B - B = 5.259 10. These B values agree with Assignment 2 by Lafferty et al. The assignment of the ground state transition and the shift of the “hot” bands toward lower frequency were substantiated by temperature-difference FTS spectra
Measurement of the D+/- production asymmetry in 7 TeV pp collisions
No full textThe asymmetry in the production cross-section \sigma of D+/- mesons, A_P = (\sigma(D+) - \sigma(D-))/(\sigma(D+) + \sigma(D-)), is measured in bins of pseudorapidity \eta and transverse momentum p_T within the acceptance of the LHCb detector. The result is obtained with a sample of D+ -> K_S pi+ decays corresponding to an integrated luminosity of 1.0 fb^-1, collected in pp collisions at a centre of mass energy of 7 TeV at the Large Hadron Collider. When integrated over the kinematic range 2.0 K_S pi+ decay is negligible. No significant dependence on \eta or p_T is observed
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