62021 research outputs found
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Learning from Experience: Librarians as Research Partners
Full text linkGiven current discussions in the field about librarians’ engagement in research partnerships, this study examines the experiences of librarians at The Ohio State University, a doctoral university with very high research (R1), to better understand their preparation for the collaborations, the knowledge and skills they contributed, their roles on project teams, their motivation for joining teams, and the subsequent criteria they have adopted for determining participation on future projects. Interview and post-interview survey findings indicate librarians have developed their own strategies for determining their roles on research teams based on their individual strengths and that they have benefited from the experiences overall. This study also reveals inconsistencies in support for this form of engagement and makes recommendations for libraries related to scoping involvement and establishing criteria for participation of librarians on research teams.Publisher allows immediate open acces
A Cutting-Edge Magnetic Immunocapture Method to Isolate Cell-Type-Specific Mitochondria from Complex Neural Tissue
Full text linkBest Undergraduate Poster (Discovery Category) - OSU CBI Research Day 2025Alterations in neuronal and glial metabolism contribute to numerous neurodegenerative diseases, and the crosstalk between these two cell types (i.e., axoglial metabolic coupling) is at the center of extensive investigation. Metabolic alterations frequently culminate in mitochondrial dysfunction, but so far it has proven challenging to obtain cell-type-specific metabolic data from neuronal or glial mitochondria in mouse models of injury or disease. Magnetic immunocapture of genetically tagged mitochondria has emerged as a powerful strategy, yet existing methods are either incompatible with sensitive downstream multi omic workflows or not yet tested in complex tissues with highly heterogeneous cell populations. Here, I refined the “MITO-Tag” approach, which leverages a Cre dependent 3×HA EGFP OMP25 epitope tag localized to the outer mitochondrial membrane. Following enzymatic and mechanical dissociation, mitochondria were rapidly immunopurified from cortical neurons, oligodendrocyte lineage cells, and—accomplished here for the first time—peripheral nerve axons using anti-HA magnetic beads in liquid chromatography-tandem mass spectrometry (LC-MS/MS)-compatible KPBS buffer. This method yields specific and structurally intact mitochondria, as confirmed by live-organelle imaging and Western blotting for compartment-specific markers (COXIV, VDAC, citrate synthase), with minimal contamination from other organelles. Proteomic analysis of brain-derived immunoprecipitates (IPs) revealed mitochondrial enrichment comparable to existing magnetic immunopurification workflows. By enabling multi-omic mitochondrial profiling from moderate-abundance cell types within complex tissues, this method provides a versatile tool for investigating mitochondrial involvement in neurological disease and injury in vivo. Notably, isolation from peripheral nerve axons now offers the ability to characterize Wallerian degeneration and axoglial metabolic coupling mechanisms at an unprecedented resolution.NIH R01NS123450-01NIH R01NS111024-02OSU College of Engineering Thesis Research Grant2025 CAN-ACN Travel GrantNo embargoAcademic Major: Biomedical Engineerin
Unsettling Acts: Performing Transnational Adoption
No full textThis book is freely available in an open access edition thanks to the generous support of Emory University and the Andrew W. Mellon Foundation
How Gremlins Opened the Door for Patent Trolls at the International Trade Commission: The ITC Subverts Its Own Mission by Turning Legal Coercion Into a "Domestic Industry" and Punishing Productive American Companies
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Exploiting Starlink LEO for PNT: signal structure and ephemeris and timing error correction
No full textThe Governance of Open Repository Programs: Progress and Possibilities
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Comic Fascism: Ideology, Catholicism, and Americanism in Italian Children's Periodicals
No full textItem embargoed for three year