58,324 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
The ALMA-PILS survey: First detection of the unsaturated 3-carbon molecules Propenal (CHCHO) and Propylene (CH) towards IRAS 162932422 B
No full textAccepted for publication in A&A. 24 pages, 17 figuresInternational audienceComplex organic molecules with three carbon atoms are found in the earliest stages of star formation. In particular, propenal (CHCHO) is a species of interest due to its implication in the formation of more complex species and even biotic molecules. This study aims to search for the presence of CHCHO and other three-carbon species such as propylene (CH) in the hot corino region of the low-mass protostellar binary IRAS 16293--2422 to understand their formation pathways. We use ALMA observations in Band 6 and 7 from various surveys to search for the presence of CH and CHCHO towards the protostar IRAS 16293--2422 B (IRAS 16293B). We report the detection of both CH and CHCHO towards IRAS 16293B, however, no unblended lines were found towards the other component of the binary system, IRAS 16293A. We derive column density upper limits for CH, HCCCHO, n-CHOH, i-CHOH, CO, and cis-HC(O)CHO towards IRAS 16293B. We then use a three-phase chemical model to simulate the formation of these species in a typical prestellar environment followed by its hydrodynamical collapse until the birth of the central protostar. Different formation paths, such as successive hydrogenation and radical-radical additions on grain surfaces, are tested and compared to the observational results. The simulations reproduce the abundances within one order of magnitude from those observed towards IRAS 16293B, with the best agreement found for a rate of cm s for the gas-phase reaction C + O C + CO. Successive hydrogenations of C, HC(O)CHO, and CHOCHO on grain surfaces are a major and crucial formation route of complex organics molecules, whereas both successive hydrogenation pathways and radical-radical addition reactions contribute to the formation of CHCHO
Gonioctena (Brachyphytodecta) mauroi Cho & Borowiec 2016
No full textGonioctena (Brachyphytodecta) mauroi Cho & Borowiec, 2016 (Figs 11, 13) Gonioctena (Brachyphytodecta) mauroi Cho & Borowiec, 2016a: 176 (original description). Type locality. ‘ Vietnam, Lang Son Province, Loc Binh, Mt. Mau Son’. Type material examined. HOLOTYPE: ♂, ‘Tonkin, Montes Mauson, April, Mai 2-3000’, H. Fruhstorfer // HOLOTYPUS Gonioctena (Brachy.) mauroi sp. n. Cho & Borowiec 2015’ (LMCM). Description. See CHO & BOROWIEC (2016a). Differential diagnosis. Gonioctena (B.) mauroi is very similar to G. (B.) longshengensis sp. nov. and G. (B.) medvedevi in body shape, coloration and setose aedeagus. From these two species, G. (B.) mauroi can be distinguished only by aedeagus subparallel-sided with apical process widest in middle and slightly narrower than median lobe (subparallel-sided with apical process broad, widest in apical 1/3 and subequal to median lobe in width in G. (B.) longshengensis sp. nov. and moderately narrowed with apical process moderately widened to lateral tooth-like projections near apex in G. (B.) medvedevi). Gonioctena (B.) kuatunensis sp. nov. differs in semicircular apex of aedeagus (triangular or subtriangular in other species). Distribution. Vietnam: Lang Son Province (Fig. 13).Published as part of Cho, Hee-Wook, 2017, Two new species of the Gonioctena mauroi species-group from China (Coleoptera: Chrysomelidae: Chrysomelinae), pp. 173-181 in Acta Entomologica Musei Nationalis Pragae 57 (1) on page 180, DOI: 10.1515/aemnp-2017-0066, http://zenodo.org/record/531615
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
A copy of a letter from B. H. Obert (August 30, 1913)
No full textThis is copy of letter written by B. H. Obert, the Secretary of the Board of Directors of the YMCA in Ashbury Park New jersey. The letter is a recommendation for Nam Pok Cho. It is dated August 30, 1913
Search for direct CP violation in D0→h−h+ modes using semileptonic B decays
No full textA search for direct CP violation in D0 → h-h+ (where h = K or π) is presented using data corresponding to an integrated luminosity of 1.0 fb-1 collected in 2011 by LHCb in pp collisions at a centre-of-mass energy of 7 TeV. The analysis uses D0 mesons produced in inclusive semileptonic b-hadron decays to the D0μX final state, where the charge of the accompanying muon is used to tag the flavour of the D0 meson. The difference in the CP-violating asymmetries between the two decay channels is measured to be ΔACP = ACP(K-K+) - ACP(π-π+) = (0.49± 0.30 (stat) ± 0.14 (syst))%
Miscellaneous correspondence from the H. B. Clawson papers, 1872-1895
No full textMiscellaneous Correspondence from the H. B. Clawson papers, 1872-1895: (1) Letter dated 24 December 1872 at Salt Lake City, Utah, by Robert Williams to Hiram B. Clawson, thanking him for gifts (1 page); (2) Letter dated 6 May 1878 at Liverpool, England, by Charles Nibley to Bradley Clawson (1 page); (3) Letter dated 21 December 1886? at Chicago, Illinois, by B. R. Wells of M.D. Wells & Company to Hiram B. Clawson, regretting missing him when he was in town (1 page); (4) Letter dated 2 March 1881 at Middlefield [State unknown] by Matthew Smith to "Mrs. Clawson" (Ellen S. Clawson, Hiram\u27s first wife) (2 pages); (5) Letter dated 29 January 1884 at South Bend, Indiana, by Mrs. G. Foote (probably mother of James Foote, who married Ellen\u27s daughter Georgia) to Mrs. [Ellen S.] Clawson, prior to the expected visit of Ivie Clawson (2 pages); (6) Letter dated 21 April 1881 at New York City by Titus B. Eldridge to H. B. Clawson, upon receiving a gift of the Book of Mormon (2 pages); (7) Letter dated 18 August 1891 at Hayden, Idaho, by "Ed" to Ivie Clawson at Soda Springs, Idaho, describing his travels from Salt Lake City through Idaho; (8) Letter dated 26 April 1892 at East Mill Creek by Nellie Fisher to Ellen Clawson, with questions about the history of "Primary" (2 pages); (9) Letter dated 29 March 1894 at San Francisco, California, by Florence [no surname given], to her cousin Ivie Clawson at Salt Lake City, Utah (4 pages); (10) Letter dated 23 August 1894 at Paris, Idaho, by Lilian Spencer to her cousin Ivie Clawson at Salt Lake City (3 pages); (11) Letter dated 1 January 1895 at Boston, Massachusetts, by Jean C. Thatcher to Ivie Clawson Greene, congratulating her on her marriage (pages); (12) Letter dated 13 April 1895 by Henry F. CLark, Manager of the Literary Bureau, Curtis Publishing Company, at Philadelphia, Pennsylvania, to E. C. Clawson, providing information on author Edward Bellamy; (13) Letter dated 6 April [no year] by John T. White to Ivie and Winnie Clawson; (14) Letter [undated] by Mary DeVol (?) at Council Bluffs to Mrs. [Ellen S.] Clawso
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Endocytosis of fluorescent Dylight 659-bradykinin free H-kininogen by CHO cells.
No full text<p>CHO-K1 and CHO-745 cells were grown on cover slips, and the lysosomes/endosomes were labeled with 0.5 μM LT Green in Ham F-12 serum-free medium containing 50.0 μM Zn<sup>2+</sup> for 20 min at 37°C and analyzed by confocal fluorescence microscopy. Dylight 659-bradykinin free H-kininogen (200 nM, red) endocytosis and intracellular localization are indicated by the colocalization with acidic vesicles labeled with LT Green. Normal endocytosis by CHO-K1: (A) LT Green; (B) Dylight 659- bradykinin free H-kininogen; (C) merged images and diphasic contrast. Normal endocytosis by CHO-745: (D) LT Green; (F) Dylight 659- bradykinin free H-kininogen; (G) merged images and diphasic contrast.</p
High-precision CTE Measurement System for the Structural Materials in Space Applications
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