890 research outputs found
Herne Bay 1830-1880 a failed seaside resort?
No full textThis thesis sets out to examine Herne Bay’s success or otherwise as a seaside resort in the period during the nineteenth century, with a specific focus on the period from 1830 until around 1880. The significance of these dates centres upon the involvement of speculators and the building of the first deep sea pier that opened in 1832 closely followed by the passing of an Improvement Act in 1833. The effect of the 1833 Act was to provide a form of governance over the town’s affairs with varying effectiveness until this was reformed in the early 1880s as a result of provisions contained within the Public Health Act 1875. This time period also includes important transport developments that had a significant effect upon the town
PEG-mediated and Agrobacterium-mediated transformation in the mycopathogen Verticillium fungicola
No full textVerticillium fungicola, a severe mycopathogen of the cultivated mushroom Agaricus bisporus, was successfully transformed using both PEG-mediated and Agrobacterium-mediated techniques. PEG-mediated co-transformation was successful with hygromycin B resistance (hph), uidA (β-glucuronidase GUS), and green fluorescent protein (GFP) genes. Agrobacterium-mediated transformation was successful with the hph gene. Transformation frequencies of up to 102 transformants per μg DNA and 4068 transformants per 105 conidia were obtained for PEG-mediated and Agrobacterium-mediated transformation respectively. Expression of integrated genes in co-transformants was stable after 18 months of successive sub-culturing on non-selective medium, and following storage at -80 °C in glycerol. Molecular analysis of PEG-mediated transformants showed integration of the transforming genes into the target genome. Molecular analysis of Agrobacterium-mediated transformants showed integration of transforming DNA as single copies within the target genome. Co-transformants exhibited symptoms of disease in inoculation experiments and were at least as virulent as the wild-type fungus. GFP and GUS expression were observed in-vivo with the GFP-tagged strain showing great potential as a tool in epidemiological and host-pathogen interaction studies. The development of transformation systems for V. fungicola will allow in-depth molecular studies of the interaction of this organism with A. bisporus
Computational data from: "Maximising recombination across macadamia populations to generate linkage maps for genome anchoring"
No full text<p>Computational files associated with the publication “Maximising recombination across macadamia populations to generate linkage maps for genome anchoring” (not yet published – further details to be provided when published).</p><p>Data Processing: Single nucleotide polymorphism (SNP) and dominant markers were identified using the DArT Pty Ltd. genome complexity reduction method. Genetic linkage maps were constructed using JoinMap v5. Visual representations of each map were generated using MapChart v2.3. A complete description of the methods is provided in “Maximising recombination across macadamia populations to generate linkage maps for genome anchoring” (Langdon et al. unpublished – manuscript under review)</p><p>Excel spreadsheet containing information on a series of Genetic linkage maps. Marker id, genetic position and the sequence associated with the marker for each map is provided. Visual representation of each genetic linkage map (using the program MapChart v2.3) is provided as a PDF.</p><p>Data generated was established by Kirsty S Langdon, Graham J King, Abdul Baten, Ramil Mauleon, Peter C Bundock, Bruce L Topp and Catherine J Nock</p><p>Leaf samples were collected by Kirsty Langdon, Catherine Nock and Asuka Kawamata from Southern Cross University and Mobashwer Alam from the University of Queensland.</p>
Author Correction: A portrait of the Higgs boson by the CMS experiment ten years after the discovery
No full textIn the version of this article initially published, CMS Collaboration author names, affiliations and acknowledgements were omitted and have now been included in the HTML and PDF versions of the articl
A phenomenological study of siblings bereaved by suicide: A shared experience
No full textThis interpretative phenomenological analysis explored the key issues in the grief experiences of seven young adults bereaved by the youth suicide of a sibling. We conducted semi-structured phone interviews from which we derived four themes describing the participants’ experiences of: (a) the process of grief, (b) grief interactions (within families and outside), (c) continuing bonds, and (d) meaning-making and growth through grief. The stories highlight the impact of family relationships on the grieving process in siblings and the need for support to help family members better communicate, understand, and respect each other’s needs as they process their grief.Griffith Health, School of Applied PsychologyNo Full Tex
Genotyping by allele-specific PCR
No full textSNPs are the basis for many polymorphisms that are detected using systems such as restriction fragment length polymorphisms (RFLPs), randomly amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphisms (AFLPs). Cleaved amplified polymorphic site (CAPS or PCRRFLP) markers have been adopted by a number of groups to enable mapping of SNP markers identified in ESTs. Because this system relies on each SNP being associated with a restriction enzyme site, only a proportion of SNPs are amenable to CAPS. In addition, the enzyme digest step is both time-consuming and often unreliable. An alternative method is allele-specific PCR (AS-PCR). The reliability of AS-PCR can be increased by the addition of destabilizing mismatches within the allele-specific primer (ASP), reducing the false positive rate, and a parallel positive control PCR reducing false negatives. The positive control PCR takes place in the same tube as the diagnostic PCR, competing with it for access to polymerase and nucleotides and this too may contribute to a reduction in the false positive rate. The outcomes of these experiments allowed the formation of guidelines which were successfully used to design assays for three additional SNPs. Briefly, the guidelines were as follows: (i) the melting temperature (Tm) of the positive control primers should be 20-25°C lower than that of the 300-1000 bp PCR product itself; (ii) the Tm of the inner PCR products should be - 35°C lower than the positive control PCR product; and (iii) the annealing temperature should be 20°C below the Tm of the positive control PCR product. The recommended optimization pathway involved alterations to both positive control and ASP concentrations
Measurements of the charge asymmetry in top-quark pair production in the dilepton final state at s√=8 TeV with the ATLAS detector
No full textSee paper for full list of authors - 27 pages plus author list + cover pages (45 pages total), 8 figures, 4 tables, submitted to Physical Review D, All figures including auxiliary figures are available at this http URLInternational audienceMeasurements of the top--antitop quark pair production charge asymmetry in the dilepton channel are presented using data corresponding to an integrated luminosity of 20.3 fb−1 from pp collisions at a center-of-mass energy of s√=8 TeV collected with the ATLAS detector at the Large Hadron Collider at CERN. Inclusive and differential measurements as a function of the invariant mass, transverse momentum, and longitudinal boost of the tt¯ system are performed both in the full phase space and in a fiducial phase space closely matching the detector acceptance. Two observables are studied: AℓℓC based on the selected leptons and Att¯C based on the reconstructed tt¯ final state. The inclusive asymmetries are measured in the full phase space to be AℓℓC=0.008±0.006 and Att¯C=0.021±0.016, which are in agreement with the Standard Model predictions of AℓℓC=0.0064±0.0003 and Att¯C=0.0111±0.0004
Enrichment of genomic DNA for polymorphism detection in a non-model highly polyploid crop plant
No full textLarge polyploid genomes of non-model species remain challenging targets for DNA polymorphism discovery despite the increasing throughput and continued reductions in cost of sequencing with new technologies. For these species especially, there remains a requirement to enrich genomic DNA to discover polymorphisms in regions of interest because of large genome size and to provide the sequence depth to enable estimation of copy number. Various methods of enriching DNA have been utilised, but some recent methods enable the efficient sampling of large regions (e.g. the exome). We have utilised one of these methods, solution-based hybridization (Agilent SureSelect), to capture regions of the genome of two sugarcane genotypes (one Saccharum officinarum and one Saccharum hybrid) based mainly on gene sequences from the close relative Sorghum bicolor. The capture probes span approximately 5.8 megabases (Mb). The enrichment over whole-genome shotgun sequencing was 1011-fold for the two genotypes tested. This level of enrichment has important consequences for detecting single nucleotide polymorphisms (SNPs) from a single lane of Illumina (Genome Analyzer) sequence reads. The detection of polymorphisms was enabled by the depth of sequence at or near probe sites and enabled the detection of 270 000280 000 SNPs within each genotype from a single lane of sequence using stringent detection parameters. The SNPs were present in 13 00016 000 targeted genes, which would enable mapping of a large number of these chosen genes. SNP validation from 454 sequencing and between-genotype confirmations gave an 87%91% validation rate
Search for a fermiophobic Higgs boson in the diphoton decay channel with the ATLAS detector
No full textA search for a fermiophobic Higgs boson using diphoton events produced in proton-proton collisions at a centre-of-mass energy of s=7 TeV is performed using data corresponding to an integrated luminosity of 4.9 fb−1 collected by the ATLAS experiment at the Large Hadron Collider. A specific benchmark model is considered where all the fermion couplings to the Higgs boson are set to zero and the bosonic couplings are kept at the Standard Model values (fermiophobic Higgs model). The largest excess with respect to the background-only hypothesis is found at 125.5 GeV, with a local significance of 2.9 standard deviations, which reduces to 1.6 standard deviations when taking into account the look-elsewhere effect. The data exclude the fermiophobic Higgs model in the ranges 110.0–118.0 GeV and 119.5–121.0 GeV at 95 % confidence level
Differential LongSAGE tag abundance analysis in a barley seed germination time course and validation with relative real-time RT-PCR
No full textA Long Serial Analysis of Gene Expression (LongSAGE) approach was employed to identify changes in mRNA transcript abundance in a time course of malting barley (Hordeum vulgare L.). Statistical analyses confidently identified 57 LongSAGE sequence tags as having significant changes in abundance between points in the time course. Eight of the genes which correspond to these tags were targeted for validation by relative real-time reverse transcriptase PCR (RT-PCR) analysis. Each gene was analysed by SYBR (R) Green detection in grain sampled at each of four time points from dry seed to grain germinated to 120 h post-steeping. Among the genes examined are alpha-amylase type B, a key starch degrading enzyme involved in germination (1-3,1-4)-beta-D-glucanase, the major cell wall degrading enzyme, and cysteine proteinase EP-B, an important enzyme of proteolysis in barley seed germination. These three transcripts show significant up-regulation at 48 h post-steeping and remain significantly elevated throughout malting. These results provide transcriptional data to support the understanding of how the relative rates of protein and carbohydrate modification contribute to malting and brewing. mRNA abundance levels observed using real-time RT-PCR show good correlation with the data obtained from the LongSAGE study. This confirms the sensitivity of detection obtainable with SAGE technology and validates the patterns of change of transcript abundance exhibited by some key genes for barley seed germination. (c) 2008 Elsevier Ireland Ltd. All rights reserved
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