25,349 research outputs found
Batido de kale: alimento funcional
Objetivo: Determinar la composición química y el grado de aceptación de un batido nutricional a base de kale, y el grado de información acerca de los alimentos antioxidantes en los alumnos de cuarto año de la Lic. en Nutrición de la Universidad FASTA de la ciudad de Mar del Plata en el año 2021.
Materiales y Métodos: La investigación se divide en tres etapas: durante la primera, el estudio es cuasi experimental, ya que se diseñó un batido a base de KALE con diferentes proporciones de este ingrediente, las cuales se dieron a degustar a tres profesionales, analizando las variaciones en sus características organolépticas; una segunda etapa, donde se procede a analizar el batido de kale elegido por el panel de expertos, en un laboratorio de alimentos de la ciudad de Mar de Plata; y una tercera etapa de tipo descriptiva, que tiene como finalidad la medición de variables en una población definida, presentando los rasgos característicos de un fenómeno analizado, evaluando la aceptabilidad del producto por parte de los estudiantes de 4to año de la Licenciatura en Nutrición de la Universidad FASTA y su beneficio nutricional.Fil: Di Iulio, Valeria B. Universidad FASTA. Facultad de Ciencias Médicas; Argentina.Fil: del Valle Viglione, Lisandra. Universidad FASTA. Facultad de Ciencias Médicas; Argentina.Fil: Minnaard, Vivian. Universidad FASTA. Facultad de Ciencias Médicas. Departamento de Nutrición; Argentina
Brassica oleracea var. acephala (kale) improvement by biological activity of root endophytic fungi
12 páginas, 3 tablas, 6 figurasBrassica oleracea var. acephala (kale) is a cruciferous vegetable widely cultivated for its leaves and fower buds in Atlantic Europe and the Mediterranean area, being a food of great interest as a "superfood" today. Little has been studied about the diversity of endophytic fungi in the Brassica genus, and there are no studies regarding kale. In this study, we made a survey of the diversity of endophytic fungi present in the roots of six diferent Galician kale local populations. In addition, we investigated whether the presence of endophytes in the roots was benefcial to the plants in terms of growth, cold tolerance, or resistance to bacteria and insects. The fungal isolates obtained belonged to 33 diferent taxa. Among those, a Fusarium sp. and Pleosporales sp. A between Setophoma and Edenia (called as Setophoma/Edenia) were present in many plants of all fve local populations, being possible components of a core kale microbiome. For the frst time, several interactions between endophytic fungus and Brassica plants are described and is proved how diferent interactions are benefcial for the plant. Fusarium sp. and Pleosporales sp. B close to Pyrenophora (called as Pyrenophora) promoted plant growth and increased cold tolerance. On the other hand, isolates of Trichoderma sp., Pleosporales sp. C close to Phialocephala (called as Phialocephala), Fusarium sp., Curvularia sp., Setophoma/Edenia and Acrocalymma sp. were able to activate plant systemic resistance against the bacterial pathogen Xanthomonas campestris. We also observed that Fusarium sp., Curvularia sp. and Setophoma/Edenia confered resistance against Mamestra brassicae larvae.Tis research was financially supported by project RTI2018-096591-B-I00 34 (MCIU/AEI/FEDER, UE). JP has a contract from the Xunta de Galicia IN607A2016/13. Authors want to thank Dr. Rieta Gols (Laboratory of Entomology, Wageningen University, Wageningen, Te Netherlands) for providing us with Mamestra eggs and for the unvaluable inputs in this manuscript.Peer reviewe
Periodical UV-B radiation hormesis in biosynthesis of kale sprouts nutraceuticals
The objective of the present study was to evaluate the periodical UV-B radiation hormesis during kale seeds germination in their main content of secondary metabolite compounds (phenols; glucosinolates; total antioxidant capacity –TAC–) and their changes during a refrigerated shelf-life. The total UV-B doses received were 0, 5, 10, and 15 kJ m−2 (CTRL, UVB5, UVB10, and UVB15) in where the 25% was applied on the 3rd, 5th, 7th, and 10th sprouting day. UV radiation did not affect the morphological development of the sprouts. UVB10 and UVB15 treatments increased their phenolic content (>30%). Likewise, TAC was increased by UV-B lighting ~10% (DPPH) and ~20% (FRAP). The hydroxycinnamic acid content in UVB15-treated sprouts increased by 52%, while UVB5 reported an increase of 34% in the kaempferol-3,7-di-O-glucoside concentration, compared to CTRL. After 10 d at 4 °C of shelf-life, content of gallic acid hexoside I and gallic acid increased by 55 and 78% compared to UV-untreated kale sprouts, respectively. Glucoraphanin was the main glucosinolate found in kale sprouts and seeds, followed by 4-hydroxy-glucobrassicin, whose biosynthesis was enhanced by UVB10 (~24 and ~27%) and UVB15 (~36 and ~30%), respectively, compared to CTRL. In conclusion, periodical low UV-B illumination represents a useful tool to stimulate phytochemicals biosynthesis in kale sprouts as an important source of bioactive compounds with potential health benefits.Noelia Castillejo was funded by a predoctoral grant (FPU16/04763) from the Spanish Ministry of Education. Lorena Martínez-Zamora's contract (21322/PDGI/19) is part of the Regional Programme for research talent and its employability of the Seneca Foundation, co-financed by the European Social Fund (ESF) through the Youth Employment Initiative (YEI). The technical assistance of Francisca Andreo is highly appreciated
Branching fraction and CP asymmetry of the decays B+→K0Sπ+ and B+→K0SK+
An analysis of B+ → K0
Sπ+ and B+ → K0
S K+ decays is performed with the LHCb experiment. The pp
collision data used correspond to integrated luminosities of 1 fb−1 and 2 fb−1 collected at centre-ofmass
energies of
√
s = 7 TeV and
√
s = 8 TeV, respectively. The ratio of branching fractions and the
direct CP asymmetries are measured to be B(B+ → K0
S K+
)/B(B+ → K0
Sπ+
) = 0.064 ± 0.009 (stat.) ±
0.004 (syst.), ACP(B+ → K0
Sπ+
) = −0.022 ± 0.025 (stat.) ± 0.010 (syst.) and ACP(B+ → K0
S K+
) =
−0.21 ± 0.14 (stat.) ± 0.01 (syst.). The data sample taken at
√
s = 7 TeV is used to search for
B+
c
→ K0
S K+ decays and results in the upper limit ( fc · B(B+
c
→ K0
S K+
))/( fu · B(B+ → K0
Sπ+
)) <
5.8 × 10−2 at 90% confidence level, where fc and fu denote the hadronisation fractions of a ¯b
quark
into a B+
c or a B+ meson, respectively
Cloning and sequence analysis of the defective in anther dehiscence1 (DAD1) gene fragment of Chinese kale
To clone the defective in anther dehiscence1 (DAD1) gene fragment of Chinese kale, about 700 bp product was obtained by PCR amplification using Chinese kale genomic DNA as the template and a pair of specific primers designed according to the conserved sequence of DAD1 genes of Arabidopsis thaliana and Brassica rapa. The amplified product was ligated into the T vector and sequenced. The results show that the gene fragment was 678 bp long without introns. It shared 89% identity with the nucleotide sequence of the DAD1 gene of A. thaliana and the sequence identity was as high as 97 to 99% with those of other plants belonging to the same genus as Chinese kale. The amino acid sequence deduced from the nucleotide sequence had 91% identity with that of A. thaliana. It was shown that the cloned fragment was a part of Chinese kale DAD1 gene.Key words: Chinese kale, Brassica oleracea var. alboglabra, defective in anther dehiscence1 (DAD1), gene clone
Agronomic and Metabolomic Side-Effects of a Divergent Selection for Indol-3-Ylmethylglucosinolate Content in Kale (<i>Brassica oleracea</i> var. <i>acephala</i>)
Brassica oleracea var. acephala (kale) is a cruciferous vegetable widely cultivated for its leaves and flower buds in Europe and a food of global interest as a “superfood”. Brassica crops accumulate phytochemicals called glucosinolates (GSLs) which play an important role in plant defense against biotic stresses. Studies carried out to date suggest that GSLs may have a role in the adaptation of plants to different environments, but direct evidence is lacking. We grew two kale populations divergently selected for high and low indol-3-ylmethylGSL (IM) content (H-IM and L-IM, respectively) in different environments and analyzed agronomic parameters, GSL profiles and metabolomic profile. We found a significant increase in fresh and dry foliar weight in H-IM kale populations compared to L-IM in addition to a greater accumulation of total GSLs, indole GSLs and, specifically, IM and 1-methoxyindol-3-ylmethylGSL (1MeOIM). Metabolomic analysis revealed a significant different concentration of 44 metabolites in H-IM kale populations compared to L-IM. According to tentative peak identification from MS interpretation, 80% were phenolics, including flavonoids (kaempferol, quercetin and anthocyanin derivates, including acyl flavonoids), chlorogenic acids (esters of hydroxycinnamic acids and quinic acid), hydroxycinnamic acids (ferulic acid and p-coumaric acid) and coumarins. H-IM kale populations could be more tolerant to diverse environmental conditions, possibly due to GSLs and the associated metabolites with predicted antioxidant potential
Measurement of the ratio of branching fractions B(B0→K∗0γ )/B(B0s→φγ ) and the directCP asymmetry inB 0→K∗0γ
The ratio of branching fractions of the radiative B decays B0→K⁎0γ and B0s→ϕγ has been measured using an integrated luminosity of 1.0 fb−1 of pp collision data collected by the LHCb experiment at a centre-of-mass energy of s√=7TeV. The value obtained is
B(B0→K⁎0γ)B(B0s→ϕγ)=1.23±0.06(stat.)±0.04(syst.)±0.10(fs/fd),
where the first uncertainty is statistical, the second is the experimental systematic uncertainty and the third is associated with the ratio of fragmentation fractions fs/fd. Using the world average value for B(B0→K⁎0γ), the branching fraction B(B0s→ϕγ) is measured to be (3.5±0.4)×10−5.
The direct CP asymmetry in B0→K⁎0γ decays has also been measured with the same data and found to be
ACP(B0→K⁎0γ)=(0.8±1.7(stat.)±0.9(syst.))%.
Both measurements are the most precise to date and are in agreement with the previous experimental results and theoretical expectations
Nonstrangulating small colon obstruction caused by a submucosal haematoma
S. Stahel, C. B. Riley, M. Wichtel and P.-Y. Daous
Analysis of combining ability and heredity parameters of glucosinolates in Chinese kale
The study was carried out with six Chinese kale lines as materials. Complete diallel crossing was designed with 6 × 6 to calculate the combining ability and the main genetic parameters. The results are as follows: The GCA effect of P1, P2 and P5 was excellent. They were used as parants to get hybrids, the heterosis of anti-cancer glucosinolates of their hybrid was very high. By analyzing the SCA effects, 1 × 2 was an outstanding hybrid. The broad sense heritability of the main GSs were relatively higher, and the narrow sense heritability were lower, so the potential of heterosis was bigger.Keywords: Chinese kale, glucosinolate, combining ability, heritability, heterosi
Measurement of the branching fraction
The B
0
s
→ J/ψK
0
S
branching fraction is measured in a data sample corresponding to 0.41 fb−1
of integrated luminosity collected with the LHCb detector at the LHC. This channel is sensitive to
the penguin contributions affecting the sin 2β measurement from B
0
→ J/ψK
0
S
. The time-integrated
branching fraction is measured to be B(B
0
s
→ J/ψK
0
S
) = (1.83±0.28)×10−5
. This is the most precise
measurement to date
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