3,765 research outputs found
Nuclear magnetic resonance structure of d(GCATATGATAG)·d(CTATCATATGC): A consensus sequence for promoters recognized by s(K) RNA polymerase
The three-dimensional structure of d(GCATATGATAG)·d(CTATCATATGC), from the promoter region of a gene regulating sporulation in Bacillus subtilis mother cells, was determined utilizing two-dimensional nuclear Overhauser effect (2D NOE) and double-quantum-filtered COSY (2QF-COSY) spectra. To minimize the effect of methods used to obtain restraints and refine structure, several variables were studied. Interproton distance bounds were calculated very conservatively by running the complete relaxation matrix program MARDIGRAS hundreds of times using 2D NOE spectra for exchangeable and for nonexchangeable protons at different mixing times, assuming different overall correlation times and different starting structures. The 435 distance restraints were used with two different structural refinement methods: restrained molecular dynamics (rMD) and restrained Monte Carlo calculations (rMC). Refinement using different procedures and starting structures resulted in essentially the same structure (<0.8 Å rmsd), indicating that the structure is defined by experimental restraints and not the refinement method or variables used. R factors indicate the structures fit the experimental NOE data very well. Some helical parameters, notably large negative X displacement, are characteristic of A-DNA, but others are characteristic of B-DNA. As with TG·CA steps in other duplex DNA sequences studied in our laboratory, the two TG·CA steps have a positive roll, with T6-G7 exhibiting the largest, and consequently a bent helix axis. The converged structure represents a time-averaged structure. However, multiple conformations, especially in deoxyriboses, were evident from vicinal coupling constants obtained from quantitative simulations of 2QF-COSY cross-peaks and from persistent inconsistencies in experimental distances due to nonlinear conformational averaging
First observation of the decay Bs0→K*0K*0
The first observation of the decay B0s→K∗0K∗0 is reported using 35 pb−1 of data collected by LHCb in proton–proton collisions at a centre-of-mass energy of 7 TeV. A total of 49.8±7.5 B0s→(K+π−)(K−π+) events are observed within ±50 MeV/c2 of the B0s mass and 746 MeV/c2 < mKπ < 1046 MeV/c2, mostly coming from a resonant B0s→K∗0K∗0 signal. The branching fraction and the CP-averaged K∗0 longitudinal polarization fraction are measured to be B(B0s→K∗0K∗0)=(2.81±0.46(stat.)±0.45(syst.)±0.34(fs/ fd))×10−5 and fL =0.31±0.12(stat.)±0.04(syst.)
Nuclear Magnetic Resonance Structure of d(GCATATGATAG)·d(CTATCATATGC) : A Consensus Sequence for Promoters Recognized by σKRNA Polymerase
The three-dimensional structure of d(GCATATGATAG)·d(CTATCATATGC), from the promoter region of a gene regulating sporulation in Bacillus subtilis mother cells, was determined utilizing two-dimensional nuclear Overhauser effect (2D NOE) and double-quantum-filtered COSY (2QF-COSY) spectra. To minimize the effect of methods used to obtain restraints and refine structure, several variables were studied. Interproton distance bounds were calculated very conservatively by running the complete relaxation matrix program MARDIGRAS hundreds of times using 2D NOE spectra for exchangeable and for nonexchangeable protons at different mixing times, assuming different overall correlation times and different starting structures. The 435 distance restraints were used with two different structural refinement methods: restrained molecular dynamics (rMD) and restrained Monte Carlo calculations (rMC). Refinement using different procedures and starting structures resulted in essentially the same structure (<0.8 Å rmsd), indicating that the structure is defined by experimental restraints and not the refinement method or variables used. R factors indicate the structures fit the experimental NOE data very well. Some helical parameters, notably large negative X displacement, are characteristic of A-DNA, but others are characteristic of B-DNA. As with TG·CA steps in other duplex DNA sequences studied in our laboratory, the two TG·CA steps have a positive roll, with T6-G7 exhibiting the largest, and consequently a bent helix axis. The converged structure represents a time-averaged structure. However, multiple conformations, especially in deoxyriboses, were evident from vicinal coupling constants obtained from quantitative simulations of 2QF-COSY cross-peaks and from persistent inconsistencies in experimental distances due to nonlinear conformational averaging
Measurement of the ratio of branching fractions B(B0→K∗0γ )/B(B0s→φγ ) and the directCP asymmetry inB 0→K∗0γ
The ratio of branching fractions of the radiative B decays B0→K⁎0γ and B0s→ϕγ has been measured using an integrated luminosity of 1.0 fb−1 of pp collision data collected by the LHCb experiment at a centre-of-mass energy of s√=7TeV. The value obtained is
B(B0→K⁎0γ)B(B0s→ϕγ)=1.23±0.06(stat.)±0.04(syst.)±0.10(fs/fd),
where the first uncertainty is statistical, the second is the experimental systematic uncertainty and the third is associated with the ratio of fragmentation fractions fs/fd. Using the world average value for B(B0→K⁎0γ), the branching fraction B(B0s→ϕγ) is measured to be (3.5±0.4)×10−5.
The direct CP asymmetry in B0→K⁎0γ decays has also been measured with the same data and found to be
ACP(B0→K⁎0γ)=(0.8±1.7(stat.)±0.9(syst.))%.
Both measurements are the most precise to date and are in agreement with the previous experimental results and theoretical expectations
Effective lifetime measurements in the B-s(0) -> K+K-, B-0 -> K+pi(-) and B-s(0) -> pi K-+(-) decays
Measurements of the effective lifetimes in the View the MathML source, B0→K+π− and View the MathML source decays are presented using 1.0 fb−1 of pp collision data collected at a centre-of-mass energy of 7 TeV by the LHCb experiment. The analysis uses a data-driven approach to correct for the decay time acceptance.
This is the most precise determination to date of the effective lifetime in the View the MathML source decay and provides constraints on contributions from physics beyond the Standard Model to the View the MathML source mixing phase and the width difference ΔΓs
Managing Emotions in the Polish Parliament. The Marshal’s Role
Artykuł koncentruje się na analizie działań podejmowanych przez marszałka/marszałków Sejmu w celu opanowania emocji, które pojawiają się na sali sejmowej w trakcie obrad. Autor skupił się na interakcyjnych technikach (zarówno formalnych, jak i nieformalnych) wykorzystywanych przez marszałków w celu skutecznego przeprowadzenia obrad sejmowych. Celem artykułu jest analiza środków, jakie może zastosować marszałek, aby emocje nie zakłóciły procesu decyzyjnego ciała ustawodawczego.This paper analyzes actions taken by the Marshals of the Polish Parliament to control the emotions that appear in the parliamentary hall during the meeting and debates. The author has focused on interactional techniques (both formal and informal) used by the marshals to conduct the parliamentary debate in the most effective way. This article aims to analyze techniques, which are at Marshallʼs disposal in order to prevent Parliamentary debates from getting disturbed by emotions.Udostępnienie publikacji Wydawnictwa Uniwersytetu Łódzkiego finansowane w ramach projektu „Doskonałość naukowa kluczem do doskonałości kształcenia”. Projekt realizowany jest ze środków Europejskiego Funduszu Społecznego w ramach Programu Operacyjnego Wiedza Edukacja Rozwój; nr umowy: POWER.03.05.00-00-Z092/17-00.
Monografia została dofinansowana ze środków Wydziału Ekonomiczno-Socjologicznego UŁ (grant nr B1511200000856.02) oraz przez Fundację Uniwersytetu Łódzkiego
Branching fraction and CP asymmetry of the decays B+→K0Sπ+ and B+→K0SK+
An analysis of B+ → K0
Sπ+ and B+ → K0
S K+ decays is performed with the LHCb experiment. The pp
collision data used correspond to integrated luminosities of 1 fb−1 and 2 fb−1 collected at centre-ofmass
energies of
√
s = 7 TeV and
√
s = 8 TeV, respectively. The ratio of branching fractions and the
direct CP asymmetries are measured to be B(B+ → K0
S K+
)/B(B+ → K0
Sπ+
) = 0.064 ± 0.009 (stat.) ±
0.004 (syst.), ACP(B+ → K0
Sπ+
) = −0.022 ± 0.025 (stat.) ± 0.010 (syst.) and ACP(B+ → K0
S K+
) =
−0.21 ± 0.14 (stat.) ± 0.01 (syst.). The data sample taken at
√
s = 7 TeV is used to search for
B+
c
→ K0
S K+ decays and results in the upper limit ( fc · B(B+
c
→ K0
S K+
))/( fu · B(B+ → K0
Sπ+
)) <
5.8 × 10−2 at 90% confidence level, where fc and fu denote the hadronisation fractions of a ¯b
quark
into a B+
c or a B+ meson, respectively
Measurement of the effective B0s→K+K− lifetime
A measurement of the effective lifetime is presented using approximately 37 pb−1 of data collected by LHCb during 2010. This quantity can be used to put constraints on contributions from processes beyond the Standard Model in the meson system and is determined by two complementary approaches as
τKK=1.440±0.096 (stat)±0.008 (syst)±0.003 (model) ps
Determinants of The Manufacturing Competitiveness of The Visegrad Group Countries
Cel – Celem artykułu jest przedstawienie wyników przeprowadzonego badania do-tyczącego determinant, pomiaru i analizy zmian konkurencyjności przetwórstwa przemysłowego krajów Grupy Wyszehradzkiej na tle wybranych państw UE ze szczególnym uwzględnieniem wymiaru środowiskowego. Metoda badań – Do realizacji celu badania zastosowano metodę analizy materiału źródłowego oraz dostępnych danych statystycznych dla wybranych lat okresu 2004–2019. Następnie dokonano formalno-statystycznej istotności kryteriów i selekcji wskaźników, a wnioskowanie dedukcyjne przeprowadzono zgodnie z wynikami analizy taksonomicznej metodą porządkowania liniowego Hellwiga. Wyniki – Na podstawie zgromadzonego materiału i przeprowadzonej analizy ustalono ranking konkurencyjności produkcji przemysłowej na podstawie miary syntetycznej. W ramach Grupy Wyszehradzkiej liderem okazały się Czechy, najsłabiej natomiast wypadła Polska. Oryginalność / wartość / implikacje / rekomendacje – Podjęta w opracowaniu tematyka czynników warunkujących konkurencyjność produkcji przemysłowej w zmieniającym się otoczeniu makroekonomicznym jest ważna i aktualna. Artykuł jest próbą uporządkowania współczesnych determinant konkurencyjności produkcji przemysłowej. Przeprowadzone badania i sformułowane wnioski wypełniają istniejącą lukę w zakresie uwzględnienia wyzwań związanych z transformacją klimatyczno-energetyczną, stanowiąc tym samym przyczynek do dalszych rozważań.Purpose – The purpose of the article is to present the results of the study on determi-nants, measurement and analysis of changes in the competitiveness of manufacturing in the Visegrad Group countries against the selected EU countries, with particular emphasis on the environmental dimension. Research method – To achieve the goal of the paper, the method of analysing the source material and the available statistical data for the selected years of the period 2004–2019 was used.Then, the formal and statistical significance of the criteria and selection of indicators was performed, and deductive inference was carried out according to the results of taxonomic analysis using the Hellwig linear ordering method. Results – On the basis of the collected material and the conducted analysis, a ranking of the manufacturing competitiveness was established based on a synthetic measure.The Czech Republic turned out to be the leader within the Visegrad Group, while Poland achieved the worst results. Originality / value / implications / recommendations – The subject of the study, i.e. the factors determining the competitiveness of manufacturing in the changing macro economic environment, is important and up -to-date.The article is an attempt to organise the contemporary determinants of manufacturing competitiveness.The conducted research and formulated conclusions fill the existing gap as regards taking into account the challenges related to the climate and energy transformation, thus contributing to further considerations.Publikacja została dofinansowana ze środków subwencji przyznanej Uniwersytetowi Ekonomicznego w Krakowie – Projekt nr 075/EEG/2022/POT.Marta ULBRYCH: [email protected] LESIAK: [email protected] ULBRYCH - Kolegium Ekonomii, Finansów i Prawa, Uniwersytet Ekonomiczny w KrakowieJakub LESIAK - Kolegium Ekonomii, Finansów i Prawa, Uniwersytet Ekonomiczny w KrakowieBąk A., 2016, Porządkowanie liniowe obiektów metodą Hellwiga i TOPSIS – analiza porównawcza, „Prace Naukowe Uniwersytetu Ekonomicznego we Wrocławiu”, nr 426(26), s. 22–231, DOI: 10.15611/pn.2016.426.02.Bąk A., 2018, Analiza porównawcza wybranych metod porządkowania liniowego, „Prace Naukowe Uniwersytetu Ekonomicznego we Wrocławiu”, nr 508(21), s. 19–28, DOI: 10.15611/pn.2018.508.02.Bianchi P., Labory S., 2018, Industrial Policy for Manufacturing Revolution. 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