123,226 research outputs found
Four and a half LIM protein 1C (FHL1C)
No full textFour-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C
A comment on "Intergenerational equity: sup, inf, lim sup, and lim inf"
No full textWe reexamine the analysis of Chambers (Social Choice and Welfare, 2009), that produces a characterization of a family of social welfare functions in the context of intergenerational equity: namely, those that coincide with either the sup, inf, lim sup, or lim inf rule. Reinforcement, ordinal covariance, and monotonicity jointly identify such class of rules. We show that the addition of a suitable axiom to this three properties permits to characterize each particular rule. A discussion of the respective distinctive properties is provided.Social welfare function; Intergenerational equity; Lim sup ; Lim inf
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Epanerchodus gangwonus Mikhaljova & Lim 2002
No full textEpanerchodus gangwonus Mikhaljova & Lim, 2002 Epanerchodus gangwonus Mikhaljova & Lim, 2002: 19 –21, 20: figs 1–8. Remarks. Originally described from Gangwon-do, South Korea (Mikhaljova & Lim, 2001), this species has since never been rediscovered. Distribution. South Korea.Published as part of M, E L E N A V., Va, I K H A L J O & Lim, Kil-Young, 2006, The millipede genus Epanerchodus Attems, 1901 in the Korean Peninsula, with a description of a new species (Diplopoda, Polydesmida, Polydesmidae), pp. 45-53 in Zootaxa 1350 on page 48, DOI: 10.5281/zenodo.17451
Three-dimensional stacking as a line intensity mapping statistic
No full textLine intensity mapping (LIM) is a growing technique that measures the integrated spectral line emission from unresolved galaxies over a three-dimensional region of the Universe. Although LIM experiments ultimately aim to provide powerful cosmological constraints via auto-correlation, many LIM experiments are also designed to take advantage of overlapping galaxy surveys, thus enabling joint analyses of two datasets. We introduce a flexible simulation pipeline that can generate mock galaxy surveys and mock LIM data simultaneously for the same population of simulated galaxies. Using this pipeline, we explore a simple joint analysis technique: three-dimensional co-addition (stacking) of LIM data on the positions of galaxies from a traditional galaxy catalogue. We test how the output of this technique reacts to changes in experimental design of both the LIM experiment and the galaxy survey, its sensitivity to various astrophysical parameters, and its susceptibility to common systematic errors. We find that an ideal catalogue for a stacking analysis targets as many high-mass dark matter halos as possible. We also find that the signal in a LIM stacking analysis originates almost entirely from the large-scale clustering of halos around the catalogue objects rather than the catalogue objects themselves. While stacking is a sensitive and conceptually simple way to achieve a LIM detection, thus providing a valuable way to validate a LIM auto-correlation detection, it will likely require a full cross-correlation to achieve further characterisation of the galaxy tracers involved, as the cosmological and astrophysical parameters we explore here have degenerate effects on the stack.
The LIM-Homeodomain Protein Islet Dictates Motor Neuron Electrical Properties by Regulating K + Channel Expression
No full textNeuron electrical properties are critical to function and generally subtype specific, as are patterns of axonal and dendritic projections. Specification of motoneuron morphology and axon pathfinding has been studied extensively, implicating the combinatorial action of Lim-homeodomain transcription factors. However, the specification of electrical properties is not understood. Here, we address the key issues of whether the same transcription factors that specify morphology also determine subtype specific electrical properties. We show that Drosophila motoneuron subtypes express different K + currents and that these are regulated by the conserved Lim-homeodomain transcription factor Islet. Specifically, Islet is sufficient to repress a Shaker-mediated A-type K + current, most likely due to a direct transcriptional effect. A reduction in Shaker increases the frequency of action potential firing. Our results demonstrate the deterministic role of Islet on the excitability patterns characteristic of motoneuron subtypes. © 2012 Elsevier Inc
Platyrrhinus guianensis Velazco & Lim, 2014, new species
No full textPlatyrrhinus guianensis new species Guianan Broad-nosed Bat Figures 4–7 P [latyrrhinus]. helleri: Lim, 1993: 162 (part) Platyrrhinus helleri: Smith and Kerry, 1996: 932 (part) Platyrrhinus helleri: Lim and Engstrom, 2000: 121 P [latyrrhinus]. helleri: Lim and Engstrom, 2001 a: 632 (part) Platyrrhinus helleri: Lim and Engstrom, 2001 b: 664 (part) Platyrrhinus helleri: Engstrom and Lim, 2002: 364 (part) Platyrrhinus helleri: Lim and Norman, 2002: 54 P [latyrrhinus]. helleri: Lim et al., 2002: 1239 (part) Platyrrhinus helleri: Lim and Engstrom, 2005: 77 (part) Platyrrhinus helleri: Lim et al., 2005 a: 244 (part) Platyrrhinus helleri: Lim et al., 2005 b: 87 (part) Platyrrhinus helleri: Clare et al., 2007: 187 (part) Platyrrhinus helleri: Borisenko et al., 2008: 475 (part) Platyrrhinus helleri: Lim, 2009: 45 (part) P [latyrrhinus]. recifinus: Velazco, 2009: 259 (part) Platyrrhinus recifinus: Tavares and Velazco, 2010: 119 (part) Platyrrhinus helleri: Clare et al., 2011: 8 (part) Platyrrhinus helleri: Clare, 2011: 4 (part) Platyrrhinus helleri: Lim, 2012: 253 (part) Platyrrhinus helleri: Lim and Tavares, 2012: 115 (part) Holotype. Dried skin, skull and skeleton of an adult pregnant female, Royal Ontario Museum (ROM) number 113465, obtained 20 September 2001 by Burton K. Lim and Zacharias Norman (original field number F 50445). The skin, skull, and skeleton are in good condition. Frozen tissues are deposited at the Royal Ontario Museum (F 50445). Type locality. Pobawau Creek mouth, 100 m; Upper Takutu-Upper Essequibo; Guyana, 3 ° 16 ’ 3.1 ”N, 58 ° 46 ’ 42.7 ”W (Fig. 3). Paratypes. The skin, skull, and skeleton of an adult male (ROM 108487) caught on 8 October 1997 at 38 mi Camp, 35 km SW Kurupukari, 100 m, Iwokrama Forest, Potaro-Siparuni, Guyana, 4 ° 22 ’W, 58 ° 51 ’W; one skin and skull of an adult male (ROM 114070) caught on 16 April 2002 and one skin, skull, and skeleton of an adult pregnant female (ROM 113991) caught on 13 April 2002 at Brownsberg Nature Park headquarters, 500 m, Brokopondo, Suriname, 4 ° 57 ’N, 55 ° 11 ’W; and the skin and skull of an adult male (ROM 114195) caught on 21 April 2002 at Km 2.4 Wittie Kreek trail, 300 m, Brownsberg Nature Park, Brokopondo, Suriname, 4 ° 56 ’N, 55 ° 10 ’W. The holotype and 4 paratypes, along with 31 other specimens from the known distributional range, are listed in Appendix 1 (Fig. 3). Measurements of each specimen of the type series of P. guianensis are provided in Table 4. Distribution. Platyrrhinus guianensis is known from Guyana and Suriname (Fig. 3). Etymology. The species name is derived from the Latin description of its endemic distribution in the Guiana region of South America. Diagnosis. Platyrrhinus guianensis is distinguished from its congeners by a combination of external and craniodental characteristics. The ventral fur is dark gray; ventral fur unicolor; dorsal stripe wide and brilliant white; fringe of hair along margin of uropatagium long, conspicuously dense, and pale yellow. The skull of P. guianensis lacks a fossa on the squamosal root of the zygomatic arch. Dentally, two stylar cuspules are present on the posterior cristid of P 4; and one stylid cuspulid on the anterior cristid of p 4. Description. Platyrrhinus guianensis is a small Platyrrhinus (FA 37–41 mm) distinguished from its sister species P. recifinus by its smaller size and shorter skull (Table 5; Velazco & Gardner 2009, Table 2–4 and 7). However, measurements of P. guianensis overlap with P. angustirostris, P. brachycephalus, P. fusciventris, P. helleri, P. i n c a r u m, and P. matapalensis (Tables 4 –5). Dorsal fur mostly dark brown, but paler on the upper dorsum; dorsal fur is bicolored with darker tips; facial stripes wide and white; dorsal stripe brilliant white; ventral fur dark gray, individual hairs unicolored; pinnae have well-marked fold lines; tragus and anterior and posterior rims of pinnae bright yellow (Fig. 4); lateral borders of the proximal half of the noseleaf and borders of the horseshoe yellow; inferior border of the horseshoe completely free of upper lip; posterior margin of uropatagium has the shape of an inverted ‘U’; hair on upper surface of feet brown, long and dense (Fig. 5); fringe of hair along the trailing margin of uropatagium long, conspicuously dense, and pale yellow; metacarpal III longer than metacarpal V. Rostrum is slender; has a well developed anterior notch in the nasals; postorbital processes moderately developed; paraoccipital processes poorly developed; two infraorbital foramina present; posterior border of hard palate ‘V’-shaped (Fig. 6); fossa on the squamosal root of the zygomatic arch absent; and paraoccipital and paracondylar processes poorly developed. Upper inner incisors bilobed and convergent, not in contact, and tips extend below level of cingula of upper canines; upper outer incisors monolobate; two stylar cuspules on posterior cristid of P 4; hypoconal basin fossa of P 4 shallow; M 1 parastyle present; M 1 mesostyle absent; M 1 metacone divided in two cones; M 1 metacone labial cingulum present; stylar cuspule absent on lingual cingulum of M 1 metacone; sulcus on posterior cristid of paracone joined to cingulum of lingual face of metacone on M 1; M 1 metastyle present; M 1 protocone well developed; M 2 parastyle present; labial cingulum present on M 2 paracone; stylar cuspule on lingual face of M 2 paracone absent; M 2 metastyle present; stylar cuspule absent on lingual face of M 2 metacone; lingual cingulum of the M 2 metacone not extending to the paracone; developed M 2 hypoconal basin; M 3 minute; labial and lingual cingulids on p 4; one stylid cuspulid on anterior cristid of p 4; two stylid cuspulids on posterior cristid of p 4; m 1 paraconid poorly developed; labial and lingual cingulids present on m 1; stylid cuspulid present on anterior cristid of m 1 protoconid; m 1 metaconid well developed; m 2 hypoconid absent; stylid cuspulid between the metaconid and protoconid poorly developed on m 2; labial and lingual cingulids present on m 2. Comparisons. Platyrrhinus guianensis can be confused with P. angustirostris, P. brachycephalus, P. fusciventris, P. he l l e r i, P. i nc a r u m, and P. matapalensis because their external and cranial measurements overlap (Table 3–4). But it can be easily distinguished from P. brachycephalus and P. matapalensis by the presence of one accessory cuspulid on the anterolingual cristid of p 4 (Fig. 7) (cuspulid lacking in P. matapalensis and two accessory cuspulids present in P. brachycephalus; Velazco 2005, fig. 27). Therefore, the following comparisons focus on differentiating P. guianensis from P. angustirostris, P. fusciventris, P. helleri, and P. incarum. Externally, ventral fur is dark gray in P. guianensis and P. angustirostris (brownish gray in P. i n c ar u m; pale gray in P. h el l e r i; brown in P. fusciventris); ventral fur unicolored in P. guianensis, P. angustirostris, P. fusciventris, and P. h el l e r i (bicolored in P. i nc a r u m); dorsal stripe wide and brilliant white in P. guianensis and P. he l l e r i (conspicuous but narrow in P. angustirostris, P. fusciventris, and P. incarum); tragus and anterior and posterior rims of pinnae bright yellow in P. guianensis, P. fusciventris, and P. helleri (whitish in P. angustirostris and P. incarum); lateral borders of the proximal half of the noseleaf and borders of the horseshoe yellow in P. guianensis, P. fusciventris, and P. he l l e r i (whitish in P. angustirostris and P. i n c ar um); posterior margin of uropatagium with a shape of an inverted ‘U’ in P. guianensis, P. angustirostris, and P. i n c ar u m (‘V’ shaped in P. fusciventris and P. helleri); fringe of hair along margin of uropatagium long, conspicuously dense, and pale yellow in P. guianensis (long, dense, and pale brown in P. helleri; long, dense, and whitish in P. fusciventris and P. i nc a r u m; short, dense, and pale brown in P. angustirostris); hair on the upper surface of feet brown, long and dense in P. guianensis, P. angustirostris, and P. i n c a r um (short and intermediate in density in P. fusciventris and P. hell eri); metacarpal III longer than metacarpal V in P. guianensis, P. angustirostris, and P. i n c a r um (metacarpals III and V subequal in P. fusciventris and P. he l l e r i). Cranially, there is a ‘V’-shaped posterior border of the hard palate in P. guianensis, P. angustirostris, P. hel leri, and P. i n c ar u m (‘V’- or ‘U’-shaped in P. fusciventris); fossa on the squamosal root of the zygomatic arch absent in P. guianensis, P. helleri, and P. i ncarum (shallow in P. angustirostris and P. fusciventris). Dentally, there are two stylar cuspules on posterior cristid of P 4 in P. guianensis, P. fusciventris, P. helleri, and P. i nc a r u m (three in P. angustirostris); stylar cuspule on lingual face of M 2 paracone absent in P. guianensis, P. angustirostris, and P. fusciventris (one stylar cuspule in P. he l l e r i and P. incarum); M 3 minute in P. guianensis and P. i n ca r u m (larger in P. h el l e r i, P. angustirostris, and P. fusciventris); one stylid cuspulid on the anterior cristid of p 4 in P. guianensis, P. fusciventris, and P. helleri (one or two in P. i n ca r u m and P. angustirostris); tall m 2 protoconid in P. guianensis, P. angustirostris, P. fusciventris, P. i n c a r um (Fig. 7) (shorter in P. helleri); hypoconid lacking on m 2 in P. guianensis, P. angustirostris, P. fusciventris, and P. helleri (poorly developed in P. i n c a r um); poorly developed stylid cuspulid between the metaconid and protoconid on m 2 in P. guianensis, P. fusciventris, and P. helleri (well developed in P. i ncarum and P. angustirostris). Natural history. Platyrrhinus guianensis has been documented from an elevational range of 60 to 500 m and is found primarily in rainforest (n= 33), but 3 individuals were netted in savanna. Of the 36 specimens examined, 16 are males and 20 females. Testes size (length by width in mm) ranged from 3 by 2 to 5 by 4. From 12 January to 9 February 2006, 8 of 10 females were pregnant with crown-rump (CR) measurements ranging from 4 to 13 mm. A female was pregnant on 13 April 2002 and another on 27 July 2009 with CR of 13 mm and 4 mm, respectively. Three females were pregnant on 20 and 21 September 2001 with CR ranging from 18 to 21 mm. A non-pregnant female was collected on 24 October 1997. A female had an embryo with CR of 26 mm collected on 31 October 2005. Two non-pregnant lactating females were caught on 8 and 11 November 1999 and one non-pregnant female was caught on 19 November 1997.Published as part of Velazco, Paúl M. & Lim, Burton K., 2014, A new species of broad-nosed bat Platyrrhinus Saussure, 1860 (Chiroptera: Phyllostomidae) from the Guianan Shield, pp. 175-193 in Zootaxa 3796 (1) on pages 181-189, DOI: 10.11646/zootaxa.3796.1.9, http://zenodo.org/record/22516
A new species of swamp-dwelling skink (Tytthoscincus) from Singapore and Peninsular Malaysia
No full textGrismer, L. Lee, Wood, Perry L., Jr, Lim, Kelvin K. P., Liang, Lim J. (2017): A new species of swamp-dwelling skink (Tytthoscincus) from Singapore and Peninsular Malaysia. Raffles Bulletin of Zoology 65: 574-584, DOI: 10.5281/zenodo.535801
Depolarization and decreased surface expression of K+ channels contribute to NSAID-inhibition of intestinal restitution
No full textNon-steroidal anti-inflammatory drugs (NSAIDs) contribute to gastrointestinal ulcer formation by inhibiting epithelial cell migration and mucosal restitution; however, the drug-affected signaling pathways are poorly defined. We investigated whether NSAID inhibition of intestinal epithelial migration is associated with depletion of intracellular polyamines, depolarization of membrane potential (Em) and altered surface expression of K+ channels. Epithelial cell migration in response to the wounding of confluent IEC-6 and IEC-Cdx2 monolayers was reduced by indomethacin (100μM), phenylbutazone (100μM) and NS-398 (100μM) but not by SC-560 (1μM). NSAID-inhibition of intestinal cell migration was not associated with depletion of intracellular polyamines. Treatment of IEC-6 and IEC-Cdx2 cells with indomethacin, phenylbutazone and NS-398 induced significant depolarization of Em, whereas treatment with SC-560 had no effect on Em. The Em of IEC-Cdx2 cells was: −38.5±1.8mV under control conditions; −35.9±1.6mV after treatment with SC-560; −18.8±1.2mV after treatment with indomethacin; and −23.7±1.4mV after treatment with NS-398. Whereas SC-560 had no significant effects on the total cellular expression of Kv1.4 channel protein, indomethacin and NS-398 decreased not only the total cellular expression of Kv1.4, but also the cell surface expression of both Kv1.4 and Kv1.6 channel subunits in IEC-Cdx2. Both Kv1.4 and Kv1.6 channel proteins were immunoprecipitated by Kv1.4 antibody from IEC-Cdx2 lysates, indicating that these subunits co-assemble to form heteromeric Kv channels. These results suggest that NSAID inhibition of epithelial cell migration is independent of polyamine-depletion, and is associated with depolarization of Em and decreased surface expression of heteromeric Kv1 channels.ID: S0006295207001931; M3: Article; Accession Number: S0006295207001931; Author: L.C. Freeman (b); Author: D.F. Narvaez (a); Author: A. McCoy (a); Author: F.B. von Stein (c); Author: S. Young (b); Author: K. Silver (a); Author: S. Ganta (b); Author: D. Koch (b); Author: R. Hunter (b); Author: R.F. Gilmour (c); Author: J.D. Lillich (a, ⁎); Affiliation: Department of Clinical Sciences, Kansas State University, Manhattan, KS 66506, United States; Affiliation: Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, United States; Affiliation: Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, United States; Keyword: Non-steroidal anti-inflammatory drugs; Keyword: Intestinal epithelial cells; Keyword: Membrane potential; Keyword: Potassium channels; Number of Pages: 12; Language: English;Source type: Electronic(1)http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S0006295207001931&site=eds-live&scope=sit
First observation of the decay Bs0→K*0K*0
No full textThe first observation of the decay B0s→K∗0K∗0 is reported using 35 pb−1 of data collected by LHCb in proton–proton collisions at a centre-of-mass energy of 7 TeV. A total of 49.8±7.5 B0s→(K+π−)(K−π+) events are observed within ±50 MeV/c2 of the B0s mass and 746 MeV/c2 < mKπ < 1046 MeV/c2, mostly coming from a resonant B0s→K∗0K∗0 signal. The branching fraction and the CP-averaged K∗0 longitudinal polarization fraction are measured to be B(B0s→K∗0K∗0)=(2.81±0.46(stat.)±0.45(syst.)±0.34(fs/ fd))×10−5 and fL =0.31±0.12(stat.)±0.04(syst.)
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