15 research outputs found
STUDY SENYAWA KIMIA DALAM FASE EKSTRAK ETIL ASETAT SIMPLISIA Cinnamomum spp. Secara KCKT dan KG-SM
No full textCinnamomum burmanii (Nees n’ Th. Nees) Blume, Cinnamomum culilaban (L.) J.S. Presl dan Cryptocarpa massoy (Oken) Kosterm are a rare plant species that grow in Indonesia. Species with this same of genus (Cinnamomum) is a potential in the field of medicine, but the comparison of the composition and concentration of the chemical content has not been known so their utilization in medical applications is not optimal. Therefore, the research was conducted in order to analyze of chemical compounds in ethyl acetate extracts phase of three plants by chromatography, They were the HPLC (High Performance Liquid Chromatography), and GC-MS (Gas Chromatography Mass Spectrometry) analysis. The analysis was done of the ethyl acetate extract phase in order to identify of chemical compounds which largely composed of essential oils. Based on the results of HPLC analysis, with using by the reference standards of eugenol showed that in Cinnamomum burmanii and Cinnamomum culilaban (L.) J.S. Presl identified contain of eugenol essential oils, 0.18% and 3.11% respectively. Based on the result of GC-MS analysis, Cinnamomum burmanii was found two components of chemical compounds main with the largest og percentage, They were sinamaldehid (90.24%) and coumarin (53.46%) compounds. On the Cinnamomum culilaban (L.) J.S. Presl was found four components of chemical compounds main, They were metileugenol (40.68%), verbanone (14.58%), terpinol (8.74%) and spathulenol (8.12%) compounds. Later in the Cryptocarpa massoy (Oken) Kosterm was found three components of chemical compounds main, They were massoyalakton (73.64%), butanoat acid (12.25%) and pentadekanoat acid (9.76%) compounds
Seleksi Mikroba Endofit Curcuma Zedoria dalam Memproduksi Senyawa Kimia Antimikroba
No full textIt is well known, that temu putih (Curcuma zedoaria Berg.), a rhizome of the Zingiberaceae, is oftenly used as medicine. In this connection, the potential secondary metabolites as anticancer and antimicrobes are produced by endophytic microbes in the plant. In this research, five endophytic microbes from the Curcuma zedoaria’s rhizome were fermented to produce compounds as antimicrobes
Chemical Constituent and Antimalarial Activity based on Inhibition of Heme Polymerization from Water Extract of Yellow Root (Arcangelisia flava L. Merr)
Full text linkIn developing countries, malaria remains a disease that can spread easily and caused death. Malaria is an infectious disease caused by Plasmodium sp involving female anopheles masquitos during its transmission. Arcangelisia flava L. Merr has been investigated earlier that it can inhibited P. falciparum growth. Method of antimalarial activity based on inhibition of heme polymerization can confirm one of the mechanism of antimalarial drugs. The aim of this research to study further antimalarial activity and IC50 based on inhibition of heme polymerization and determine the chemical constituent from water extract of A. flava L. Merr. This research was conducted through several steps, namely 1) water extraction, 2) column chromatography (SiO2 ; (i) CH2Cl2-MeOH=10:1~1:1 (ii) n-hexane: EA=1:1; CH2Cl2-MeOH=10:1~1:1), 3) antimalarial assay, 4) identification of chemical constituent using FTIR and GC-MS. Results of this research are water extract of A. flava L. Merr has IC50 601 ppm and identification of chemical constituent using FTIR and GC-MS was assumed as stigmastan.In developing countries, malaria remains a disease that can spread easily and caused death. Malaria is an infectious disease caused by Plasmodium sp involving female anopheles masquitos during its transmission. Arcangelisia flava L. Merr has been investigated earlier that it can inhibited P. falciparum growth. Method of antimalarial activity based on inhibition of heme polymerization can confirm one of the mechanism of antimalarial drugs. The aim of this research to study further antimalarial activity and IC50 based on inhibition of heme polymerization and determine the chemical constituent from water extract of A. flava L. Merr. This research was conducted through several steps, namely 1) water extraction, 2) column chromatography (SiO2 ; (i) CH2Cl2-MeOH=10:1~1:1 (ii) n-hexane: EA=1:1; CH2Cl2-MeOH=10:1~1:1), 3) antimalarial assay, 4) identification of chemical constituent using FTIR and GC-MS. Results of this research are water extract of A. flava L. Merr has IC50 601 ppm and identification of chemical constituent using FTIR and GC-MS was assumed as stigmastan
Profil Asam Amino Kolagen Larut Asam Teripang Pasir (Holothuria Scabra)
Full text linkKolagen yang bersumber dari laut memiliki sifat yang unik dibandingkan kolagen dari mamalia, diantaranya yaitu biokompatibilitas yang baik, risiko penyakit menular yang rendah, tidak ada kendala agama dan etika, berat molekul rendah, serta mudah diserap oleh tubuh manusia. Dari berbagai organisme laut, teripang diidentifikasi sebagai sumber kolagen yang tinggi karena penyusun dinding tubuhnya adalah kolagen. Pada penelitian ini, dilakukan ekstraksi kolagen dari teripang pasir (Holothuria scabra) dan analisis asam amino serta morfologi kolagen hasil ekstraksi. Analisis asam amino dilakukan menggunakan ultra-performance liquid chromatography (UPLC) sedangkan morfologi kolagen diamati menggunakan scanning electron microscopy (SEM). Kolagen larut asam hasil ekstraksi mengandung asam amino dengan konsentrasi terbesar yaitu asam glutamat (3151,62 ppm), glisin (2975,02 ppm), dan asam aspartat (2384,97) ppm. Sedangkan kandungan asam amino dengan konsentrasi terendah yaitu histidin (698,86 ppm), lisin (966,44 ppm), dan isoleusin (1072,9 ppm). Berdasarkan hasil SEM, struktur triple helix dari polipeptida kolagen masih dapat teramati, sehingga dapat dikatakan bahwa proses ekstraksi tidak merusak struktur kolagen
Ekstraksi dan Karakterisasi Parsial Kolagen Larut Asam dari Teripang Pasir (Holothuria Scabra)
Full text linkKolagen merupakan komponen protein utama yang terdapat dalam teripang. Tujuan dari penelitian ini yaitu melakukan ekstraksi dan karakterisasi parsial kolagen larut asam dari teripang pasir (Holothuria scabra). Kolagen teripang diperoleh melalui dua tahap yaitu pemisahan protein nonkolagen menggunakan NaOH 0,1 M dan ekstraksi kolagen dengan menggunakan CH3COOH 0,1 M. Karakterisasi terhadap kolagen hasil ekstraksi dilakukan menggunakan viskometer dan fourier transform infrared spectroscopy (FTIR). Hasil penelitian menunjukkan bahwa kolagen hasil ekstraksi memiliki rendemen sebesar 3,2% (bobot kering) dengan viskositas sebesar 0,9806 mPa.s. Hasil FTIR menunjukkan adanya beberapa gugus fungsi, yaitu amida A, B, 1, II, dan III berturut-turut pada bilangan gelombang 3477 cm-1, 2933 cm‑1, 1659 cm-1, 1552 cm-1, dan 1342 cm-1
Aktivitas Antimalaria Berbasis Penghambatan β-Hematin dalam Ekstrak Air Daun Jung Rahab (Baeckea frutecens L)
Full text linkMalaria merupakan penyakit menular yang disebabkan oleh parasit Plasmodium sp. yang ditularkan melalui gigitan nyamuk betina Anopheles. Jung rahab (Bruckea frutescens L) diketahui memiliki aktivitas dalam menekan pertumbuhan Plasmodium falciparum. Penelitian ini bertujuan untuk mengetahui senyawa kimia yang memiliki aktivitas sebagai antimalaria berbasis penghambatan β-hematin dalam ekstrak air daun jung rahab. Tahapan penelitian yang dilakukan adalah ekstraksi daun jung rahab dengan air, kromatografi kolom, skrining hasil fraksinasi kolom, Kromatografi lapis tipis (KLT) preparatif dan identifikasi senyawa kimia berdasarkan interpretasi spektra FT-IR dan GCMS. Hasil penelitian menunjukkan bahwa senyawa yang memiliki aktivitas sebagai antimalaria berdasarkan interpretasi spektra FTIR dan GCMS adalah kariofilen
Figure 2 from: Syahputra G, Gustini N, Bustanussalam B, Hapsari Y, Sari M, Ardiansyah A, Bayu A, Putra MY (2021) Molecular docking of secondary metabolites from Indonesian marine and terrestrial organisms targeting SARS-CoV-2 ACE-2, M pro, and PL pro receptors. Pharmacia 68(3): 533-560. https://doi.org/10.3897/pharmacia.68.e68432
No full textFigure 2 The structure of the PLpro SARS-CoV-2 receptor (PDB ID: 5TL6) along with the distribution of residues on the Ramachandran plot. The PLpro receptor structure is composed of 10 α-helix structures and 19 β-sheet structures. The PLpro receptor structure is ready to use in molecular docking simulations with 96.55% of the residue in the protein-forming region
Figure 5 from: Syahputra G, Gustini N, Bustanussalam B, Hapsari Y, Sari M, Ardiansyah A, Bayu A, Putra MY (2021) Molecular docking of secondary metabolites from Indonesian marine and terrestrial organisms targeting SARS-CoV-2 ACE-2, M pro, and PL pro receptors. Pharmacia 68(3): 533-560. https://doi.org/10.3897/pharmacia.68.e68432
No full textFigure 5 Analysis of the conformation and interaction of hydrogen bonds between ligands and receptors with a distance < 5 Å. A. ACE-2-ptilidepsin; B. PLpro -ptilidepsin; C. Mpro-ptilidepsin
Molecular docking of secondary metabolites from Indonesian marine and terrestrial organisms targeting SARS-CoV-2 ACE-2, M pro, and PL pro receptors
No full textWith the uncontrolled spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), development and distribution of antiviral drugs and vaccines have gained tremendous importance. This study focused on two viral proteases namely main protease (Mpro) and papain-like protease (PLpro) and human angiotensin-converting enzyme (ACE-2) to identify which of these are essential for viral replication. We screened 102 secondary metabolites against SARS-CoV-2 isolated from 36 terrestrial plants and 36 marine organisms from Indonesian biodiversity. These organisms are typically presumed to have antiviral effects, and some of them have been used as an immunomodulatory activity in traditional medicine. For the molecular docking procedure to obtain Gibbs free energy value (∆G), toxicity, ADME and Lipinski, AutoDock Vina was used. In this study, five secondary metabolites, namely corilagin, dieckol, phlorofucofuroeckol A, proanthocyanidins, and isovitexin, were found to inhibit ACE-2, Mpro, and PLpro receptors in SARS-CoV-2, with a high affinity to the same sites of ptilidepsin, remdesivir, and chloroquine as the control molecules. This study was delimited to molecular docking without any validation by simulations concerned with molecular dynamics. The interactions with two viral proteases and human ACE-2 may play a key role in developing antiviral drugs for five active compounds. In future, we intend to investigate antiviral drugs and the mechanisms of action by in vitro study
Potensi Ekstrak Kapang Endofit Asal Rimpang Kunyit Sebagai Antimalaria dan Antioksidan
Full text linkMalaria infection is still a public health problem, especially in developing countries, including Indonesia. Malaria infection is usually accompanied by increased free radicals in the body of the patient. This situation will cause decreasing the immune system of the patient. Explorating of new drugs that have antimalarial and also antioxidant activity is very important. Therefore, this study aims to determine in vitro antimalarial and antioxidant activity of endophytic fungi extract origin turmeric from Sukabumi. This study begins with fermentation process of endophytic fungi on the broth medium and ethyl acetate was used to extract the filtrat to gain the test extracts. Heme polymerization inhibition and free radical DPPH scavenging method were used to antimalarial and antioxidant in vitro assay respectively. The results showed that 18 isolates of endophytic fungi have antimalarial activity and one isolate inactive and also all isolates have antioxidant activity. Smi.Cl.6F isolate was the most active isolate on the antimalarial and antioxidant assay with IC50 value of each assay were 1.93 mg/mL and 32.28 mg/L respectively. Therefore, Smi.Cl.6F isolates potentially be used as new antimalarial drug
