Journals of Badan Penelitian dan Pengembangan Kesehatan
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Pengaruh Variasi Gliseril Monostearat pada Krim Ekstrak Kulit Buah Naga Merah Super (Hylocereus costaricensis) dan Uji Antioksidannya
No full textA Glyceryl monostearate can be used as an emulsifier because it can affect the increase in antioxidant activity. The study aims to determine the effect of fluctuations in the concentration of glyceryl monostearate (GMS) on the physical properties of cream preparations containing 70% ethanol extract from the skin of super red dragon fruit and their antioxidant activity. The research methods carried out experimentally include the manufacture of extracts from super red dragon fruit peel, phytochemical screening using thin layer chromatography, formulations using various concentrations of glyceryl monostearate 8%, 10%, 12% then the physical quality of the cream and antioxidant activity test using the DPPH method. The results of one-way ANOVA for pH, dispersion, adhesion, and viscosity tests showed a p-value of> 0.05, indicating that there was no significant difference. The IC50 results obtained with equations 1, 2, and 3 are 3.69 mg/L, respectively. 3.98mg/L; and 4.78mg/L. Formula 3 is the best formula and estimates the antioxidant activity of Vitamin C, which is a positive control, at a value of 5.83 mg/L. The results of the irritation test showed that none of the formulations caused irritation. The preference test results showed that the respondents liked all the formulas. The conclusion of this study is that increased fluctuations in the concentration of glyceryl monostearate did not affect the physical properties of super red dragon fruit skin extract cream, but did affect its antioxidant activity
Penggunaan Deksametason pada Pasien COVID-19: Systematic Review dan Meta-analisis
Full text linkCOVID-19 is associated with disseminated lung damage in patients, Dexamethasone can reduce lung injury caused by inflammation and there reduce the progression to respiratory failure and prevent death. This systematic review aimed to determine the benefits and safety of Dexamethasone in COVID-19 treatment. The study was performed by a comprehensive literature search which were published in several databases i.e., PubMed, Science Direct, VHL Regional Portal, and ClinicalTrials.gov within the search time of 28 November 2020. Inclusion criteria were articles on the study on COVID-19 patients who received Dexamethasone, observational and experimental studies on the outcomes use evaluation of Dexamethasone. Exclusion criteria are the articles that do not provide control in controlled studies and do not show clear research results on the use of Dexamethasone. An initial search from four databases by entering keywords resulted in 1,046 articles. After screening articles duplication we obtained 835 studies. Finally, 6 articles were obtained after we screened for the article that it can be obtained its full text and 5 articles joined in articles included in the meta-analysis. The analysis showed that Dexamethasone in Covid-19 patients could reduce the incidence of death within 28 days with RR of 0.78 (95% CI 0.57–0.97 P=0.13) compared with Methylprednisolone, Dexamethasone was compared without corticosteroids with RR 0.89 (95% CI 0.82-0.97 P=0.01). Dexamethasone also reduced mechanical ventilator use during treatment with RR 0.95 (95% CI = 0.86-1.05 P = 0.28) compared without corticosteroids. The conclusion from these results: the use of Dexamethasone can reduce the number of deaths in COVID-19 patients, especially severe and critically ill category patients
Evaluasi Penggunaan Ramipril dan Candesartan pada Pasien Rawat Inap COVID-19 dengan Komorbid Hipertensi di RSUD Dr. Moewardi
Full text linkHypertension is the most common comorbid in patients infected by COVID-19. Drugs often given for the treatment of hypertension, namely ramipril, and candesartan, are thought to increase the development of COVID-19 because the angiotensin-converting enzyme inhibitors (ACEI) and angiotensin receptor blockers (ARBs) increase the expression of ACE-2, which is the binding site of SARS-CoV-2. This study aims to analyze the effect of using ramipril and candesartan on healing in hospitalized patients with COVID-19 with hypertension at RSUD dr. Moewardi in 2020. This study was conducted retrospectively. The results were presented descriptively and used medical record data of hospitalized patients with COVID-19 with comorbid hypertension at RSUD dr. Moewardi in 2020. Using ramipril and candesartan affects patient recovery in terms of length of stay (LOS), symptom relief, and RT-PCR test results. Analysis of the research data using SPSS with Spearman correlation test. The Spearman correlation test showed that there was a fairly strong relationship between the use of ramipril and candesartan in patients with COVID-19 with comorbid hypertension on LOS, symptom relief, and RT-PCR test results. The study results show that using ramipril and candesartan can improve the recovery of patients with COVID-19 with comorbid hypertension. ACEI and ARB drugs have not been proven to worsen the patient's condition so their use can be continued
Efektivitas Gel Ekstrak Etanol 70% Daun Anggrek Oncidium (Oncidium Aliceara alice) terhadap Penyembuhan Luka Kulit Dorsum Tikus Sprague Dawley
Full text linkWounds are tissue damage that is generally caused by physical contact. Wound healing is a complex mechanism involving biochemical and biocellular processes. Various therapies can be done to speed up the wound healing process, one of which is treatment using natural ingredients. One of the plants that have the potential to heal wounds is Oncidium Aliceara alice. The purpose of this study was to determine the phytochemical content of Oncidium Aliceara alice and its activity in wound healing when it is formulated in a gel form. This research is experimental research with extraction stages, phytochemical assay, gel formulations, quality test, and wound healing activity tests in animals. The gel formulation was made with various concentrations of Oncidium Aliceara alice extract 2.5%, 5% and 10%. Male Sprague Dawley rats were given an incision wound on the dorsum of the longitudinal axis along 3 cm. Wound care by administering topical Bioplacenton as a positive control, base gel as a negative control, and gel extract concentration variants according to each treatment group. Based on the research, the results of qualitative phytochemical tests showed that the 70% ethanol extract of Oncidium Aliceara alice contains flavonoids (catechins), tannins, steroids, and saponins. The best results were seen on the 7th day of observation for the 10% gel extract treatment group with more perfect signs of wound closure than the bioplacenton treatment
Efektivitas Filtrat Daun Cengkih (Syzygium aromaticum) sebagai Repellent terhadap Lalat Rumah (Musca domestica) dengan Metode Space Spraying
Full text linkThe house fly is a mechanical vector for spreading diseases. Controlling flies using chemical repellents continuously could results in resistance to flies. The other alternative is using clove leave filtrate as a natural repellent. The content of clove leaves that have function as a repellent are: eugenol, saponins, and flavonoids. The purpose of this study was to analyze the effectiveness of clove leaf filtrate as a natural repellent against house flies. The space spraying method was carried out with an air diffuser and exposed for 6 hours. This type of research uses a simple quasi-experiment with a post test only control group design. The study used clove leaf concentrations of 10%, 15%, 20%, aquadest, and was replicated 6 times. The number of house flies used are 600. The result showed that the average flies that refused bait on the control was 28%, the concentration of 10% was 61.3%, 15% was 84%, and 20% was 91.3%. The results of the One Way Anova test, stated the p-value of 0.000 (<0.05), which means that there is a difference in the average number of house flies that refuse bait.The conclusion of this study is that clove leaf filtrate is effective as a house fly repellent
Uji Resistensi Vektor Demam Berdarah Dengue (DBD) Terhadap Insektisida Permethrin di Kota Binjai Provinsi Sumatera Utara Tahun 2022
Full text linkThe control effort of Dengue Hemorrhagic Fever (DHF) which is transmitted by the Aedes sp. infected with the Dengue virus, of which can be done by fogging. The active ingredient for fogging generally uses the insecticide permethrin. This study aimed to identify the resistance status of DHF vectors to the insecticide permethrin in controlling DHF vectors. The method of collecting Aedes sp. larvae was carried out in 100 residents' houses in neighborhoods 3 and 4 of Damai Village, North Binjai District, Binjai City, North Sumatra Province by observing each water reservoir that has the potential to become a breeding ground for Aedes sp. Aedes sp. larvae colonized until the F1 generation, then tested for resistance to permethrin insecticide using the CDC Bottle method. Data analysis was carried out descriptively. The percentage of death of test mosquitoes was 25% (<80%). It was concluded that the Aedes sp. in Neighborhoods 3 and 4 of Damai Village, North Binjai District, Binjai City were resistant to the insecticide permethrin. It is recommended that the Binjai City Health Office/Government make a policy in controlling the DHF vector by replacing Permethrin insecticide with another class of insecticides
Pengaruh Pemberian Kombinasi Ekstrak Etanol Piper crocatum dan Ekstrak Akuades Elaeocarpus ganitrus pada Kadar Gula Tikus
Full text linkDiabetes mellitus is a disease characterized by hyperglycemia. Antidiabetic research using natural ingredients has been carried out a lot, one of which is by utilizing red betel leaves and granite leaves. Combining two plants must be done to increase the antihyperglycemic effect and reduce the toxic effects. This study aimed to obtain the most effective dose variations in combinations of plants that are safer and have better results so that they can be used as alternative treatments. The blood sugar levels were measured in vivo on white Wistar rats induced with streptozotocin using a glucometer. The results showed that a single extract of red betel leaf (100 mg/kg BW) and a single extract of granite leaf (100 mg/kg BW) had a percentage decrease in blood sugar levels of 27% and 10%, respectively. While the combination of extracts with a ratio of 100:100, 50:50, 100:50, and 50:100 mg/kg BW has a decrease in blood sugar levels of 50%, 42%, 33%, and 19% after 21 days of observation. The study also showed that the positive control of 0.036 mg glimepiride caused a 36% decrease in sugar levels. Statistical analysis proved that the effect of reducing blood sugar levels on days 0 to 21 differed for each treatment group with a p-value of 0.05. The study concluded that the combination of red betel leaf extract and granite leaf extract was more effective on blood sugar levels than the single extract of each plant in white male Wistar rats. The percentage reduction in blood sugar levels of the combination extract with a dose ratio of 50:50 mg/kg BW and 100:100 mg/kg resulted in better antidiabetic activity than the positive control glimepiride. The combination ratio of 100:100 mg/kg BW extract had the best antidiabetic activity compared to the other extract treatment groups
Analisis Spasial Pengaruh House Index dengan Kasus DBD di Kecamatan Bangilan Kabupaten Tuban
Full text linkDengue Hemorrhagic Fever (DHF) in Tuban Regency was an important health problem. Bangilan sub-district is a sub-district in Tuban Regency which has high dengue cases. The Bangilan Health Center has not used mapping or spatial analysis in its surveillance activities for dengue cases. Disease mapping is useful in assisting surveillance activities and making it easier for us to find out the spread of the house index in each village area. The purpose of this study is to analyze the spatial house index with dengue cases in Bangilan District, Tuban Regency. This type of research is descriptive analytic. The population in this study is a house around DHF patients with a radius of 100 m from the patient's house in Bangilan District, with a total sample of 774 houses. The research variables were cases of DHF and house index. DHF cases in 2020-2022 were obtained from secondary data from the Bangilan Health Center, while the house index was obtained from the larvae survey results. The data were analyzed to determine the effect of the house index and dengue cases using the GeoDa application. The results showed that the average house index in Bangilan District was 20.8% which according to WHO was high. The pattern of spread of DHF cases is random with an Average Nearest Neighbor value = 1 and the results of Moran's Index (I) = 0.061, meaning that there is a strong spatial autocorrelation effect on the house index with DHF cases
Peran Pengambil Kebijakan dan Masyarakat dalam Pencegahan Penularan Filariasis (Studi Kualitatif)
Full text linkFilariasis is still a health problem in East TanjungJabung and Belitung regencies even though mass treatment activities have been carried out for five rounds. Both districts are still endemic for filariasis because the Microfilaria rate (Mf rate) is still more than 1%. This study aims to identify the role of the community of various parties in controlling filariasisin Tanjung Jabung Timur and Belitung regencies. The research design is descriptive with a qualitative approach. Informants for the in-depth interviews were health workers at the district, sub-district and village levels as well as the village community. The number of health worker informants was 10 people, 16 community leaders, 16 cadres, and 9 sufferers, while the FGD consisted of 4 groups. Each group consists of 7 participants. Data was collected through in-depth interviews and group discussions and the data were analyzed qualitatively. Recruitment of informants for determining in-depth interviews using the snow ball method. The results showed that the roles of officers and policy makers in TanjungJabung Timur Regency were quite good, but in Belitung Regency they were less involved. Meanwhile, the role of community leaders in Indonesia is still not optimal in controlling filariasisespecially in disseminating information about filariasis. Therole of community leaders in East Tanjung Jabung Regency is more powerful than Belitung Regency. Filariasis control activities still need support from policy makers in allocating budgets and the role of community leaders to mobilize the community to be actively involved in controlling filariasis
Pengembangan dan Validasi Metode Bioanalisis Trimetoprim dalam Sampel Plasma dan Urin Manusia Simulasi Menggunakan KCKT-PDA
Full text linkTo monitor trimethoprim levels (TMP) in humans, a validated, simple, and cost-efficient analytical method is needed to be applied regularly. However, most of the methods used to establish TMP levels in urine and plasma use complex instrumentation. Therefore, in this study, a simpler High-Performance Liquid Chromatography-based (HPLC) method was developed. The separation was carried out using a GIST® C18 column (150 × 4.6 mm, 5 μm) at a temperature of 35°C which was fed by a mobile phase in the form of an acetic acid solution pH 2.5: acetonitrile (87:13, v / v) at a speed of 1.4 ml/min. Detection was performed with Photodiode Array Detector (PDA) at wavelengths of 254 nm and 243 nm to quantify TMP in urine and plasma samples respectively. The preparation of urine and plasma sequentially was carried out by the liquid-liquid extraction (ECC) method using ethyl acetate and the protein precipitation using acetonitrile. This method proved to be selective, linear (R=0.997), accurate with %error ≤ 10.29% at LLOQ level and above LLOQ value %error ≤ 10.45%, precision with %RSD ≤ 11.79% at LLOQ level and %RSD ≤ 10.82% above LLOQ. In addition, this method is quite sensitive for pharmacokinetic studies in the urine and monitoring of TMP levels in the blood with LLOQ 5 mg/L in both urine and plasma. The stability of trimethoprim in solution, urine, and plasma was conducted to ensure storage time. The developed method is proven to be valid and can be applied in pharmacokinetic studies and monitoring of trimethoprim drug levels in urine and plasma