3,410 research outputs found
Metabolomic and genomic investigation into the actinomycete genus <i>Planomonospora</i>
No full textBacteria belonging to the order of Actinomycetales are the source of many clinically important drugs, especially antibiotics. Due to the large back-catalogue of molecules isolated from these bacteria, it is difficult to find novel compounds by using traditional methods and sources. In this thesis, we use state-of-the-art metabolomics and genomics techniques to identify new molecules from the “rare actinomycete” genus Planomonospora. In chapter 2, we discuss methods for the differentiation of known and unknown molecules, called dereplication, in detail. Further, we describe the creation of a computationally predicted tandem mass fragmentation library from a proprietary metabolite database, which can speed up and automate the identification of known compounds. In chapter 3, we discuss our study into the “rare actinomycete” genus Planomonospora. We correlate phylogenetic diversity with metabolite diversity, showing that specific groups of strains produce specific molecules. Further, we sequence the genomes of selected strains to gain insight into their theoretical biosynthetic capacity. Eventually, we establish criteria to rationally select metabolites with a high probability of novelty, for consecutive isolation. In chapter 4, we describe the characterization of a family of novel metabolites, prioritized in the previous study. Investigation of the biosynthetic pathway suggests that the metabolite is a ribosomally synthesized and post-translationally modified peptide. Indeed, we prove that a minimal, two-gene biosynthetic gene cluster is responsible for the production of the compound. One of the two genes is, to our knowledge, the shortest peptide-encoding gene ever reported. This work can be the steppingstone for future investigations into the under-appreciated genus Planomonospora in particular and “rare actinomycetes” in general
Authority and Personality in M.M. Bakhtin\u27s Author and Hero in Aesthetic Activity
No full textM.M. Bakhtin\u27s fundamental claim in his seminal essay Author and Hero in Aesthetic Activity situates verbal action as the most essential constituent of human personality. A careful reading of this text reveals important truths about the relationship between free individual personhood and the nature of the speech utterance. Bakhtin connects the human experience of speech to the life and person of Jesus Christ emphasizing the incarnation and the Trinitarian view of God as essential principles for understanding the creative power of the word and consequent liabilities. Bakhtin develops these theological and philosophical coordinates around a discussion of the author-hero relationship in the novel asserting that the verbal utterance is creatively involved in building and sustaining the inner personhood of those it addresses. Bakhtin\u27s critical conclusion substantiates that from whom a word is received, and to whom the spoken word appeals has weighty influence on the type and character of human personality, and that personality\u27s relationship to authority
Metabolomic and genomic investigation into the actinomycete genus <i>Planomonospora</i>
No full textBacteria belonging to the order of Actinomycetales are the source of many clinically important drugs, especially antibiotics. Due to the large back-catalogue of molecules isolated from these bacteria, it is difficult to find novel compounds by using traditional methods and sources. In this thesis, we use state-of-the-art metabolomics and genomics techniques to identify new molecules from the “rare actinomycete” genus Planomonospora. In chapter 2, we discuss methods for the differentiation of known and unknown molecules, called dereplication, in detail. Further, we describe the creation of a computationally predicted tandem mass fragmentation library from a proprietary metabolite database, which can speed up and automate the identification of known compounds. In chapter 3, we discuss our study into the “rare actinomycete” genus Planomonospora. We correlate phylogenetic diversity with metabolite diversity, showing that specific groups of strains produce specific molecules. Further, we sequence the genomes of selected strains to gain insight into their theoretical biosynthetic capacity. Eventually, we establish criteria to rationally select metabolites with a high probability of novelty, for consecutive isolation. In chapter 4, we describe the characterization of a family of novel metabolites, prioritized in the previous study. Investigation of the biosynthetic pathway suggests that the metabolite is a ribosomally synthesized and post-translationally modified peptide. Indeed, we prove that a minimal, two-gene biosynthetic gene cluster is responsible for the production of the compound. One of the two genes is, to our knowledge, the shortest peptide-encoding gene ever reported. This work can be the steppingstone for future investigations into the under-appreciated genus Planomonospora in particular and “rare actinomycetes” in general
Documento Bancario, M.M. Mayence & Cie., Paris, 1908 Junio 14
No full textabstract: Handwritten letter from Rubén Darío to M.M. Mayence & Cie, informing about one meeting with the Spain's King and the location of Nicaraguan legation in Madrid.The original Rubén Darío Papers 1882-1945 (MSS-339) are located at ASU Libraries Archives & Special Collections. For more information about visiting the collection see http://hdl.handle.net/2286/L.A.0.The top margin has the hand written word "Particular" ("Private, Personal").In the left top margin apparently are written the words "Documento 267" ("Documento 267").Heading the letter there is a reference to a person called Santiago, whose last name hasn't been identified
M.M. Arkas: through the correlation between professional and patriotic
No full textУ статті розглянуто проблему співвідношення у творчому спадку М.М. Аркаса,
композитора, історика, громадського діяча, професійних і патріотичних засад. Проаналізовано
життєдіяльність М.М. Аркаса та виявлені основні чинники, що вплинули на формування його
світоглядних уявлень і врешті сприяли становленню у представника грецького роду українського
менталітету.В статье рассматривается проблема соотношения в творческом наследии Н.Н. Аркаса, композитора,
историка и общественного деятеля, профессионализма и патриотизма. Анализируется жизнедеятельность
Н.Н. Аркаса и выявляются основные компоненты, что способствовали формированию его духовного космоса
и становлению у представителя греческого рода украинского менталитета.The article examines the issues of rating the professional and patriotic constituents in the creative legacy of
M.M. Arkas, - the composer, historian and social activist. By researching into the life of M.M. Arkas, the author uncovers
the key factors and events that influenced the formation of his spiritual cosmos and thus eventually conduced
to moulding the Ukrainian mentality in a member of the Greek kinship
M.M. Arkas: through the correlation between professional and patriotic
No full textУ статті розглянуто проблему співвідношення у творчому спадку М.М. Аркаса,
композитора, історика, громадського діяча, професійних і патріотичних засад. Проаналізовано
життєдіяльність М.М. Аркаса та виявлені основні чинники, що вплинули на формування його
світоглядних уявлень і врешті сприяли становленню у представника грецького роду українського
менталітету.В статье рассматривается проблема соотношения в творческом наследии Н.Н. Аркаса, композитора,
историка и общественного деятеля, профессионализма и патриотизма. Анализируется жизнедеятельность
Н.Н. Аркаса и выявляются основные компоненты, что способствовали формированию его духовного космоса
и становлению у представителя греческого рода украинского менталитета.The article examines the issues of rating the professional and patriotic constituents in the creative legacy of
M.M. Arkas, - the composer, historian and social activist. By researching into the life of M.M. Arkas, the author uncovers
the key factors and events that influenced the formation of his spiritual cosmos and thus eventually conduced
to moulding the Ukrainian mentality in a member of the Greek kinship
M.M. Arkas: through the correlation between professional and patriotic
No full textУ статті розглянуто проблему співвідношення у творчому спадку М.М. Аркаса,
композитора, історика, громадського діяча, професійних і патріотичних засад. Проаналізовано
життєдіяльність М.М. Аркаса та виявлені основні чинники, що вплинули на формування його
світоглядних уявлень і врешті сприяли становленню у представника грецького роду українського
менталітету.В статье рассматривается проблема соотношения в творческом наследии Н.Н. Аркаса, композитора,
историка и общественного деятеля, профессионализма и патриотизма. Анализируется жизнедеятельность
Н.Н. Аркаса и выявляются основные компоненты, что способствовали формированию его духовного космоса
и становлению у представителя греческого рода украинского менталитета.The article examines the issues of rating the professional and patriotic constituents in the creative legacy of
M.M. Arkas, - the composer, historian and social activist. By researching into the life of M.M. Arkas, the author uncovers
the key factors and events that influenced the formation of his spiritual cosmos and thus eventually conduced
to moulding the Ukrainian mentality in a member of the Greek kinship
Plant introduction, conservation and enrichment of biodiversity in M.M. Gryshko National Botanical Garden of the NAS of Ukraine
No full textThe paper deals with the main achievements of scientists of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG) in scientific, conservation, cultural and educational activities. The results of researches on biodiversity, conservation, plants protection, introduction, acclimatization and selection, allelopathy, medical botany, landscape design, bioindication and hemosystematycs are given. It is noted that over 14 thousands species, forms and varieties of plants, are collected in NBG, and the most unique collections attributed to the objects of national heritage. Breeders have obtained more than 300 certificates or author ship on plant varieties. NBG carries out extensive international cooperation, several new exposition of plant collection appeared in Botanical Garden. In recent years the achievements of NBG scientists were awarded by two State Awards in the field of science and technology of Ukraine
Perception of M.M. Bakhtin in the West: some general remarks
No full textIn the present work is a translation of the short introductory work of Turin’s Professor Roberto Salizzoni dedicated to the analysis of art of M.M. Bakhtin and some reflections of the author of the translation, which led him to assess creativity Russian thinker, present in this book of the Italian scientist. First of all, the author of the translation does not agree with the disparaging assessment of creativity of Bakhtin how unoriginal and more abounding plagiarism. The author of the translation argues injustice exaggerated influence on the concept of Bakhtin from literary trends and ideas, including by the media those of the so-called “circle of Bakhtin”. The necessity of considering of creativity of Russian thinker as philosophical, creativity independent and original. Such a conclusion can fail and approval of Neokantian origin of “philosophical roots” of Bakhtin’s concept of philosophy of culture
Author Correction: the Influence of Nano Filter Elements on Pressure Drop and Pollutant Elimination Efficiency in Town Border Stations
No full textThe original version of this Article contained an error in the order of the author names, which was incorrectly given as Hamed Ebadiyan, Saeed Zeinali Heris, Seyed Borhan Mousavi, Shamin Hosseini Nami ; Mousa Mohammadpourfard. Consequently, in the Author Contributions section, “H.E. Investigation. S.Z.H. Supervision, Conceptualization, Methodology, Validation. S.B.M. Formal analysis, Writing original draft. S.H.N. Formal analysis, Writing original draft. M.M. Validation.” now reads: “S.Z.H. Supervision, Conceptualization, Methodology, Validation. H.E. Investigation. S.B.M. Formal analysis, Writing original draft. S.H.N. Formal analysis, Writing original draft. M.M. Validation.” The original Article has been corrected. © 2023, The Author(s)
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