345 research outputs found
Synthesis and Properties of 2′-OMe-RNAs Modified with Cross-Linkable 7‑Deazaguanosine Derivatives
No full textCross-linkable
7-deaza-6-vinylguanosine (ADVP) and 7-propynyl-7-deaza-6-vinylguanosine
(ADpVP) derivatives were synthesized and successfully incorporated
into 2′-OMe-RNA oligonucleotides by solid-phase oligonucleotide
synthesis. Analysis of their cross-link properties revealed that the
7-propynyl substituent on ADpVP induces a significant enhancement
of the cross-link kinetics of the proximal 6-vinyl group to the complementary
uracil base in the target RNA compared to that of ADVP. In addition,
the 2′-OMe-RNA oligonucleotide containing ADpVP exhibited a
higher antisense effect on luciferase production in the cell lysate
than that of ADVP. These results suggested that the 7-substituted
7-deaza-6-vinylguanosine derivatives can be used as potent cross-linkers
to target mRNA inside of cells
Pengalaman Pengguna Ome Tv Di Kalangan Remaja (Studi Fenomenologi Di SMPN 20 Kota Bengkulu)
No full text
This research was conducted to find out what the experience of Ome TV users is among teenagers, especially students at SMPN 20 Bengkulu City. This research uses descriptive qualitative research methods. Data collection was carried out through interviews, observation and documentation. The technique for taking research informants uses Purposive Sampling. The results of the research show that the experience of Ome TV users among teenagers, especially students at SMPN 20 Bengkulu City, occurs in two phases, namely: Because of Motives or past motives, namely at this stage the students use the Ome TV Application because they have a high sense of fun and curiosity. try several interesting features such as random videos to chats and In Order Motive or future motifs, namely at this stage the student uses the Ome TV application as a place to express themselves, develop English language skills and increase relations or acquaintances to invite them to play online games. . In this research, it turns out that there are many things that can improve their abilities, including developing English language skills, they are more creative and they also find friends who share their hobby in playing online games. Behind this, the author also found negative things from the impact of using the Ome TV application on students at SMPN 20 Bengkulu City, such as a lack of socializing with their classmates and finding random vulgar videos through the Ome TV application media
Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model
No full textFull author list omitted for brevity. For the full list of authors, see article.
This article is based on a previously available preprint in bioRxiv.Rigorous record-keeping and quality control are required to ensure the quality, reproducibility and value of imaging data. The 4DN Initiative and BINA here propose light Microscopy Metadata Specifications that extend the OME Data Model, scale with experimental intent and complexity, and make it possible for scientists to create comprehensive records of imaging experiments
In vitro optimization of 2 '-OMe-4 '-thioribonucleoside-modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle
No full textMicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression post-transcriptionally. Previous studies, which characterized miRNA function, revealed their involvement in fundamental biological processes. Importantly, miRNA expression is deregulated in many human diseases. Specific inhibition of miRNAs using chemically modified anti-miRNA oligonucleotides (AMOs) can be a potential therapeutic strategy for diseases in which a specific miRNA is overexpressed. 2'-O-Methyl (2'-OMe)-4'-thioRNA is a hybrid type of chemically modified oligonucleotide, exhibiting high binding affinity to complementary RNAs and high resistance to nuclease degradation. Here, we evaluate 2'-OMe-4'-thioribonucleosides for chemical modification on AMOs. Optimization of the modification pattern using a variety of chemically modified AMOs that are perfectly complementary to mature miR-21 revealed that the uniformly 2'-OMe-4'-thioribonucleoside modified AMO was most potent. Further investigation showed that phosphorothioate modification contributed to long-term miR-122 inhibition by the 2'-OMe-4'-thioribonucleoside-modified AMO. Moreover, systemically administrated AMOs to mouse using a liposomal delivery system, YSK05-MEND, showed delivery to the liver and efficient inhibition of miR-122 activity at a low dose in vivo
Patofisiologi Otitis Media Efusi (OME) Pada Pasien Dengan Refluks Laringofaringeal (LPR)
No full textOtitis media dengan efusi (OME) adalah suatu kondisi di mana terdapat efusi non-purulen pada telinga tengah tanpa disertai perforasi membran timpani. Selama beberapa dekade terakhir, refluks gastroesofageal, yang kemudian dapat berkembang menjadi refluks laringofaringeal, telah diusulkan sebagai salah satu faktor etiologi yang penting untuk OME. Sebuah studi prevalensi pada tahun 2019 menyatakan kejadian OME ditemukan 4,5 kali lebih banyak pada kelompok pasien dengan LPR dibandingkan kelompok tanpa riwayat LPR. Mekanisme patofisiologis ini sering dipertanyakan dalam penelitian terdahulu, karena terdapat berbagai hipotesis yang dapat menjelaskan pengaruh LPR terhadap patogenesis OME. Tujuan: Penulis ingin memberikan referensi baru yang lebih akurat mengenai patofisiologi terjadinya otitis media efusi pada kasus refluks laringofaringeal berdasarkan referensi-referensi yang telah dibuat sebelumnya. Metode: Pencarian literatur dilakukan dengan menggunakan database seperti PubMed, Cochrane Library, dan Science Direct dengan kata kunci “patofisiologi”, “otitis media efusi", “OME”, “refluks laringofaringeal” dan “LPR”. Kriteria inklusi dalam studi ini adalah semua studi yang membahas definisi, etiologi, epidemiologi dan mekanisme patofisiologi OME pada pasien dengan LPR. Literatur-literatur yang memenuhi kriteria inklusi dianalisis secara sistematis dan disajikan dalam bentuk artikel, tabel dan diagram yang sesuai untuk mempermudah pemahaman mengenai patofisiologi otitis media efusi pada kasus refluks laringofaringeal. Hasil: Mekanisme LPR dalam mengembangkan OME dapat dibagi menjadi beberapa kemungkinan sugestif: a) disfungsi tuba Eustachius karena LPR; b) stimulasi inflamasi di telinga tengah oleh H. pylori; dan c) aktivitas proteolitik pepsin di telinga tengah. Konten yang mengalami refluks dari gaster dapat mencapai tuba Eustachius dan menyebabkan obstruksi tuba secara langsung atau menyebabkan inflamasi, adhesi, dan kolaps pada saluran tersebut sehingga memfasilitasi mikroorganisme untuk mengembangkan OME. Di sisi lain, konten refluks yang mengandung bakteri H. pylori dan pepsin juga berperan dalam memicu respon inflamasi yang menyebabkan OME. Kesimpulan: Mekanisme yang paling mungkin menjelaskan patogenesis otitis media efusi (OME) pada kasus refluks laringofaringeal (LPR) terbagi dalam tiga proses patofisiologi utama sebagaimana telah dijelaskan sebelumnya. Pembahasan lebih lanjut mengenai upaya eradikasi H. pylori dan modifikasi gaya hidup yang bersifat preventif untuk LPR perlu dilakukan.Otitis media with effusion (OME) is a condition in which there is a non-purulent effusion in the middle ear without perforation of the tympanic membrane. Over the last few decades, gastroesophageal reflux, which can then develop into laryngopharyngeal reflux, has been proposed as one of the important etiologic factors for OME. A prevalence study in 2019 stated that the incidence of OME was found to be 4.5 times more in the group of patients with LPR than in the group without a history of LPR. This pathophysiological mechanism has often been questioned in previous studies because various hypotheses can explain the effect of LPR on the pathogenesis of OME. Objective: The author wants to provide a new, more accurate reference regarding the pathophysiology of otitis media with effusion in cases of laryngopharyngeal reflux based on previous references. Methods: A literature search was performed using databases such as PubMed, Cochrane Library, and Science Direct with the keywords "pathophysiology", "otitis media with effusion", "OME", "laryngopharyngeal reflux" and "LPR". The inclusion criteria in this study were all a study discussing the definition, etiology, epidemiology, and pathophysiological mechanisms of OME in patients with LPR. Literature that met the inclusion criteria was analyzed systematically and presented in appropriate articles, tables, and diagrams to facilitate an understanding of the pathophysiology of otitis media with effusion in cases of laryngopharyngeal reflux. Results: The mechanism of LPR in developing OME can be divided into several suggestive possibilities: a) Eustachian tube dysfunction due to LPR; b) stimulation of inflammation in the middle ear by H. pylori; and c) proteolytic activity of pepsin in the middle ear. gastric can reach the Eustachian tube and cause direct tubal obstruction a tau causes inflammation, adhesions, and collapse of the duct thereby facilitating microorganisms to develop OME. On the other hand, reflux content containing H. pylori bacteria and pepsin also plays a role in triggering the inflammatory response that causes OME. Conclusion: The mechanism that most likely explains the pathogenesis of otitis media with effusion (OME) in cases of laryngopharyngeal reflux (LPR) is divided into three main pathophysiological processes as previously described. Further discussion regarding efforts to eradicate H. pylori and lifestyle modifications that are preventive for LPR needs to be done
Patofisiologi Otitis Media Efusi (OME) Pada Pasien Dengan Refluks Laringofaringeal (LPR)
No full textOtitis media dengan efusi (OME) adalah suatu kondisi di mana terdapat efusi non-purulen pada telinga tengah tanpa disertai perforasi membran timpani. Selama beberapa dekade terakhir, refluks gastroesofageal, yang kemudian dapat berkembang menjadi refluks laringofaringeal, telah diusulkan sebagai salah satu faktor etiologi yang penting untuk OME. Sebuah studi prevalensi pada tahun 2019 menyatakan kejadian OME ditemukan 4,5 kali lebih banyak pada kelompok pasien dengan LPR dibandingkan kelompok tanpa riwayat LPR. Mekanisme patofisiologis ini sering dipertanyakan dalam penelitian terdahulu, karena terdapat berbagai hipotesis yang dapat menjelaskan pengaruh LPR terhadap patogenesis OME. Tujuan: Penulis ingin memberikan referensi baru yang lebih akurat mengenai patofisiologi terjadinya otitis media efusi pada kasus refluks laringofaringeal berdasarkan referensi-referensi yang telah dibuat sebelumnya. Metode: Pencarian literatur dilakukan dengan menggunakan database seperti PubMed, Cochrane Library, dan Science Direct dengan kata kunci “patofisiologi”, “otitis media efusi", “OME”, “refluks laringofaringeal” dan “LPR”. Kriteria inklusi dalam studi ini adalah semua studi yang membahas definisi, etiologi, epidemiologi dan mekanisme patofisiologi OME pada pasien dengan LPR. Literatur-literatur yang memenuhi kriteria inklusi dianalisis secara sistematis dan disajikan dalam bentuk artikel, tabel dan diagram yang sesuai untuk mempermudah pemahaman mengenai patofisiologi otitis media efusi pada kasus refluks laringofaringeal. Hasil: Mekanisme LPR dalam mengembangkan OME dapat dibagi menjadi beberapa kemungkinan sugestif: a) disfungsi tuba Eustachius karena LPR; b) stimulasi inflamasi di telinga tengah oleh H. pylori; dan c) aktivitas proteolitik pepsin di telinga tengah. Konten yang mengalami refluks dari gaster dapat mencapai tuba Eustachius dan menyebabkan obstruksi tuba secara langsung atau menyebabkan inflamasi, adhesi, dan kolaps pada saluran tersebut sehingga memfasilitasi mikroorganisme untuk mengembangkan OME. Di sisi lain, konten refluks yang mengandung bakteri H. pylori dan pepsin juga berperan dalam memicu respon inflamasi yang menyebabkan OME. Kesimpulan: Mekanisme yang paling mungkin menjelaskan patogenesis otitis media efusi (OME) pada kasus refluks laringofaringeal (LPR) terbagi dalam tiga proses patofisiologi utama sebagaimana telah dijelaskan sebelumnya. Pembahasan lebih lanjut mengenai upaya eradikasi H. pylori dan modifikasi gaya hidup yang bersifat preventif untuk LPR perlu dilakukan.Otitis media with effusion (OME) is a condition in which there is a non-purulent effusion in the middle ear without perforation of the tympanic membrane. Over the last few decades, gastroesophageal reflux, which can then develop into laryngopharyngeal reflux, has been proposed as one of the important etiologic factors for OME. A prevalence study in 2019 stated that the incidence of OME was found to be 4.5 times more in the group of patients with LPR than in the group without a history of LPR. This pathophysiological mechanism has often been questioned in previous studies because various hypotheses can explain the effect of LPR on the pathogenesis of OME. Objective: The author wants to provide a new, more accurate reference regarding the pathophysiology of otitis media with effusion in cases of laryngopharyngeal reflux based on previous references. Methods: A literature search was performed using databases such as PubMed, Cochrane Library, and Science Direct with the keywords "pathophysiology", "otitis media with effusion", "OME", "laryngopharyngeal reflux" and "LPR". The inclusion criteria in this study were all a study discussing the definition, etiology, epidemiology, and pathophysiological mechanisms of OME in patients with LPR. Literature that met the inclusion criteria was analyzed systematically and presented in appropriate articles, tables, and diagrams to facilitate an understanding of the pathophysiology of otitis media with effusion in cases of laryngopharyngeal reflux. Results: The mechanism of LPR in developing OME can be divided into several suggestive possibilities: a) Eustachian tube dysfunction due to LPR; b) stimulation of inflammation in the middle ear by H. pylori; and c) proteolytic activity of pepsin in the middle ear. gastric can reach the Eustachian tube and cause direct tubal obstruction a tau causes inflammation, adhesions, and collapse of the duct thereby facilitating microorganisms to develop OME. On the other hand, reflux content containing H. pylori bacteria and pepsin also plays a role in triggering the inflammatory response that causes OME. Conclusion: The mechanism that most likely explains the pathogenesis of otitis media with effusion (OME) in cases of laryngopharyngeal reflux (LPR) is divided into three main pathophysiological processes as previously described. Further discussion regarding efforts to eradicate H. pylori and lifestyle modifications that are preventive for LPR needs to be done
PHOTOISOMERIZATION DYNAMICS OF dMe-OMe-NAIP, A MODEL FOR THE RETINAL CHROMOPHORE
No full textAuthor Institution: Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706; Universit di Siena, Siena, I-53100, Italy; Universit di Siena, Siena, I-53100, Italy and Bowling Green State University, Bowling Green, OH 43403; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706The N-alkylated indanylidene pyrroline Schiff bases (NAIP) mimic the speed and efficiency of photoisomerization of retinal, whose photoisomerization is a key step in the molecular mechanism of vision. We present here a study of the ultrafast isomerization and subsequent relaxation of dMe-OMe-NAIP, a newly synthesized compound in the NAIP class with less steric congestion near the reactive double bond. We show that the excited-state dynamics of dMe-OMe-NAIP are slower than in previously studied NAIP compounds. This simpler compound also lacks the pronounced coherent vibrational motion observed in retinal and other NAIP compounds. We attribute these differences to pre-twisting about the double bond in the ground state of the previously studied compounds that is absent in dMe-OMe-NAIP. The speed of the isomerization and structure of the electronically excited potential energy surface in NAIP compounds make them attractive targets for studies of vibrationally mediated photochemistry. Previous studies from our group have shown that vibrational excitation before promotion to reactive electronic states can influence the course of reactions in isolated molecules. We seek to extend these experiments to molecules in more complicated environments, specifically the solution phase. To that end, we also present preliminary results of experiments probing the timescale of vibrational energy transfer in dMe-OMe-NAIP and other photoreactive Schiff bases
The Phenyltetraene Lysophospholipid Analog PTE-ET-18-OMe as a Fluorescent Anisotropy Probe of Liquid Ordered Membrane Domains (Lipid Rafts) and Ceramide-Rich Membrane Domains
No full textAuthor manuscript. Published in final edited form as: Biochim Biophys Acta. 2007 September; 1768(9): 2213–2221.The conjugated phenyltetraene PTE-ET-18-OMe (all-(E)-1-O-(15’-Phenylpentadeca-8’,10’,12’,14’-tetraenyl)-2-O-methyl-rac-glycero-3-phosphocholine), is a recently developed fluorescent lysophospholipid analog of edelfosine, (Quesada et al. (2004) J. Med. Chem. 47, 5333–5335). We investigated the use of this analog as a probe of membrane structure. PTE-ET-18-OMe was found to have several properties that are favorable for fluorescence anisotropy (polarization) experiments in membranes, including low fluorescence in water and moderately strong association with lipid bilayers. PTE-ET-18-OMe has absorbance and fluorescence properties similar to those of diphenylhexatriene (DPH) probes, with about as large a difference between its fluorescence anisotropy in liquid disordered (Ld) and ordered states (gel and Lo) as observed for DPH. Also like DPH, PTE-ET-18-OMe has a moderate affinity for both gel state ordered domains and Lo state ordered domains (rafts). However, unlike fluorescent sterols or DPH (Megha and London (2004) J. Biol. Chem. 279, 9997–10004), PTE-ET-18-OMe is not displaced from ordered domains by ceramide. Also unlike DPH, PTE-ET-18-OMe shows only slow exchange between the inner and outer leaflets of membrane bilayers, and can thus be used to examine anisotropy of an individual leaflet of a lipid bilayer. Since PTE-ET-18-OMe is a zwitterionic molecule, it should not be as influenced by electrostatic interactions as are other probes that do not cross the lipid bilayer but have a net charge. We conclude that PTE-ET-18-OMe has some unique properties that should make it a useful fluorescence probe of membrane structure.This work was supported by NIH grant GM 48596 to EL and a Spanish MEC grant BQU2003/04413 to AUA.Peer reviewe
Partial Hepatectomy with Middle Hepatic Vein Reconstruction Using a Left Inferior Vena Cava Graft
No full textDuplicated inferior vena cava (IVC) is a rare congenital anomaly. We describe the utility of a new graft from the left IVC in a patient with duplicated IVC for reconstructing the middle hepatic vein (MHV) after partial hepatectomy with MHV resection. A 67-year-old woman with hepatitis C was found to have a liver tumor. Magnetic resonance imaging confirmed that the tumor, which was attached to the MHV, was hepatocellular carcinoma. Central bisectionectomy (S4, S5, and S8 resection) could not be tolerated because of poor liver function and a low future liver remnant volume. Therefore, partial hepatectomy with MHV resection was performed. The left IVC was harvested as a venous graft and was substituted for the resected MHV. She recovered uneventfully and was discharged on postoperative day 12. To the best of our knowledge, this is the first report of using the left IVC as a venous graft. The left IVC is a good candidate graft for the MHV or for portal vein reconstruction because of its length, diameter, and easy harvesting (it did not require an extra incision) in a patient with duplicated IVC
In vitro optimization of 2′-OMe-4′-thioribonucleoside–modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle
No full textMicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression post-transcriptionally. Previous studies, which characterized miRNA function, revealed their involvement in fundamental biological processes. Importantly, miRNA expression is deregulated in many human diseases. Specific inhibition of miRNAs using chemically modified anti-miRNA oligonucleotides (AMOs) can be a potential therapeutic strategy for diseases in which a specific miRNA is overexpressed. 2′-O-Methyl (2′-OMe)-4′-thioRNA is a hybrid type of chemically modified oligonucleotide, exhibiting high binding affinity to complementary RNAs and high resistance to nuclease degradation. Here, we evaluate 2′-OMe-4′-thioribonucleosides for chemical modification on AMOs. Optimization of the modification pattern using a variety of chemically modified AMOs that are perfectly complementary to mature miR-21 revealed that the uniformly 2′-OMe-4′-thioribonucleoside–modified AMO was most potent. Further investigation showed that phosphorothioate modification contributed to long-term miR-122 inhibition by the 2′-OMe-4′-thioribonucleoside–modified AMO. Moreover, systemically administrated AMOs to mouse using a liposomal delivery system, YSK05-MEND, showed delivery to the liver and efficient inhibition of miR-122 activity at a low dose in vivo.journal articl
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