312,575 research outputs found
Molecular structure of highly excited resonant states in Mg-24 and the corresponding Be-8+O-16 and C-12+C-12 decays
No full textExotic Be-8 and C-12 decays from high-lying resonances in Mg-24 are analyzed in terms of a cluster model. The calculated quantities agree well with the corresponding experimental data. It is found that the calculated decay widths are very sensitive to the angular momentum carried by the outgoing cluster. It is shown that this property makes cluster decay a powerful tool to determine the spin as well as the molecular structures of the resonances.Physics, NuclearSCI(E)7ARTICLE5null8
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
Induction of c-Cbl contributes to anti-cancer effects of HDAC inhibitor in lung cancer
No full textHere we found loss of c-Cbl, an E3 ligase, expression in non-small cell lung cancer (NSCLC) compared with its adjacent normal tissue in patient specimens. HDAC inhibition by WJ or knockdown of HDAC 1, HDAC2, HDAC3 or HDAC6 all induced c-Cbl. Ectopic expression of c-Cbl induced decreased EGFR, inhibited growth in NSCLC cells. Knockdown of EGFR inhibited NSCLC growth. Mutation of EGFR at Y1045 decreased WJ-induced growth inhibition as well as in vivo anti-cancer effect and EGFR degradation mediated by WJ. Time-lapse confocal analysis showed co-localization of c-Cbl and EGFR after WJ treatment. Furthermore, WJ inhibited lung tumor growth through c-Cbl induction in orthotopic and tail vein injected models. C-Cbl up-regulation induced by HDACi is a potential strategy for NSCLC treatment
Technology Sourcing Evaluation of an Advanced Technology Industry: Taiwan’s Contactless Smart Card
No full textB -> eta K-c(eta ' K-c) decays in QCD factorization
No full textWe study the exclusive decays of the B meson into pseudoscalar charmonium states eta(c) and eta(c)' within the QCD factorization approach and find that the non-factorizable corrections to naive factorization are infrared safe at leading-twist order. The spectator interactions arising from the kaon twist-3 effects are formally power suppressed but chirally and logarithmically enhanced. An important improvement by including the O(alpha(s)) corrections is the cancellation of the renormalization scale mu dependence of the decay amplitude. However, the calculated decay rates are too small to accommodate the experimental data. On the other hand, we compare the theoretical calculations for B meson decays to J/psi, psi', eta(c) and eta'(c), and find that the predicted relative decay rates of these four states are approximately compatible with the experimental data.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000223097800007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Physics, Particles & FieldsSCI(E)17ARTICLE3365-3703
Anisotropy of fishtail effects in EuBa2Cu3Oy crystal prepared by melt-textured growth method
No full textThe behavior of critical current density in EuBa2Cu3Oy crystal prepared by melt-textured growth method were studied. The fishtail effects were observed for H parallel to C and H perpendicular to C. For H parallel to C the fishtail effects were explained by collective pinning theory, whereas for H perpendicular to C it has been attributed to matching effects.Physics, AppliedSCI(E)EI
History sketch 157th Engineer (C) Bn.
No full textThis is a historical description of the 157th Engineer (C) Battalion written from memory by author. Notes are not attached as described on last page of document
Dissect c-Maf-interacting proteins and regulation of IL-10 by c-Maf
No full text轉錄因子c-Maf是調控IL-4基因之特異性轉錄因子，並且在第二型輔助型T細胞(T helper 2 cells)的分化中扮演一個極為重要的角色。在被刺激之後，c-Maf的表現量會在Th2細胞以及巨噬細胞中大大提升，並引發大量的IL-4以及IL-10之表現，抑制IL-12的表達。因此，我們假設c-Maf之所以在不同的細胞中會有不同功能，可能是透過和不同的蛋白質有交互作用所致。用酵母菌雙雜交系統，我們實驗室已經篩選出SUMOylation的E2 conjugating enzyme Ubc9，E3 ligase PIAS1，以及蛋白酪氨酸磷酸脢PTPN22可能和c-Maf有交互作用。於是藉由螢光共振能量轉移的方法，再次確認了c-Maf的確跟這三個蛋白質有交互作用。另外一項假設是既然c-Maf可以和SUMOylation以及去磷酸脢等酵素有交互作用，暗示了c-Maf可以被SUMOylation以及磷酸化，而這些轉錄後修飾也許會對於c-Maf的轉譯能力造成影響。因此，藉由螢光酵素實驗，發現c-Maf的SUMOylation以及磷酸化突變種對於IL-10基因啟動子有較差的轉錄能力。C-Maf is an IL-4-specific transcription factor that plays a vital role in Th2 cells differentiation. Upon stimulation, c-Maf is highly upregulated in Th2 cells as well as macrophages, and induces significant IL-4 and IL-10 expression but downregulates IL-12 expression. Thus, we hypothesized that c-Maf may have different functions through interacting with cell-specific proteins in different types of cells. Using yeast two-hybrid system, our group demonstrated that c-Maf can interact with Ubc9, the SUMOylation-specific E2 conjugating enzyme, PIAS1, the SUMOylation E3 ligase, and protein tyrosine phosphatase non-receptor type22 (PTPN22). Therefore, by Fluorescence Resonance Energy Transfer (FRET) assay, the interaction between c-Maf, Ubc9, c-Maf, PIAS1, and c-Maf, PTPN22 are confirmed. Since c-Maf can interact with SUMOylation enzymes and de-phosphorylation enzyme, which suggests that c-Maf undergoes SUMOylation and phosphorylation, these post-translational modification may affect the transactivity of c-Maf. Thereafter, we found that both c-Maf SUMOylation and phosphorylation mutants have poorer transactivity on IL-10 promoter by luciferase assay.封面 i文口試委員審定書 iicknowledgement iii文摘要 ivbstract vable of Contents vihapter I Introduction ….…………………………………… 1. An overview of T helper cells ...…………………………… 1.1 The role of T helper cells in immune system …………… 1.2 Th1 cell differentiation …………..……………………… 2.3 Th2 cell differentiation …………………………………… 2I. c-Maf and its function……………………………………… 3.1 c-Maf ...…...…...…………………………………………… 3.2 The role of c-Maf in Th2 cell differentiation ……...……… 5.3 Regulation of c-Maf ……………………………………… 5II. SUMOylation and its function …………………………… 6.1 SUMOylation……………………………………………… 6.2 Ubc9 ...…...…...……………………………………………… 8.3 PIAS1 ...…...…...…………………………………………… 9V. PTPN22 and its function…………………………………… 10.1 PTPN22 ...…...…...………………………………………… 10.2 Biological functions of PTPN22 …...……………………… 10. Rationale & objects ………………………………………… 11hapter II Materials & Methods ………………………… 12art I. Experiment procedures ……………………………… 12.1 Constructions …………………………………………… 12.2 Cell culture and transfection ……………………………… 14.3 SDS-PAGE and Western Blot …………………………… 14.4 Confocal microscopy………………………………………... 14.5. Fluorescence Resonance Energy Transfer...……………… 15.6. Luciferase assay ...…………………………...…………… 17art II. Experimental Materials ……………………………… 18.1 Enzymes ……………………………….…………………… 18.2 Kits …………….…………………………………………… 18.3 Chemicals & Reagents ………….………………………… 18.4 Instruments & Software …….…………………………… 21.5 Media, Solutions & Buffers …………………….………… 22hapter III Results ……………………………….…………… 24. Confirmation of protein-protein interaction between c-Maf, Ubc9, PIAS1, and PTPN22 by confocal microscopy………………………….……….……….……….… 4I. Confirm protein-protein interaction between c-Maf, Ubc9, PIAS1, and PTPN22 by Fluorescence Resonance Energy Transfer (FRET) ……………………………….……………… 4II. Examination of whether c-Maf can undergoUMOylation by FRET………….…………………….………. 6V. Investigate IL-10 expression regulated by phosphorylated and SUMOylated c-Maf in macrophage by Luciferase Reporter Assay …….…….…………….…………….……… 8hapter IV Discussion …………………………………….… 29. c-Maf interacts with Ubc9 and PIAS1, which might retain it at the nuclear periphery. …….…….…….…….……….… 9I. c-Maf can undergo SUMO-1 SUMOylation, and SUMO-modified c-Maf may be located in PML bodies, which can thus preclude its access to active chromatin domains. …….…….…….…….…….…….…….…….….….… 0II. c-Maf interacts with SUMO4 at nuclear periphery. ………….…….….….…….….…….….…….….… 2V. c-Maf interacts with PTPN22 in the nucleus. …….…….…….….…….….…….….…….….…….…. 3. SUMOylation and phosphorylation mutants of c-Maf have different transactivity on human IL-10 promoter. …….…….…….….…….…….….….…….….……. 4ables ………………………………………………….…………… 36igures ………………………………………………….………… 40eference …………………………………………………………. 59ppendix …………………………………………………………. 6
Measurement of the ratio of prompt χ c to J / ψ production in pp collisions at √s = 7 TeV
Get PDFThe prompt production of charmonium χ c and J / ψ states is studied in proton-proton collisions at a centre-of-mass energy of √s = 7 TeV at the Large Hadron Collider. The χ c and J / ψ mesons are identified through their decays χ c → J / ψ γ and J / ψ → μ + μ - using 36 pb - 1 of data collected by the LHCb detector in 2010. The ratio of the prompt production cross-sections for χ c and J / ψ, σ (χ c → J / ψ γ) / σ (J / ψ), is determined as a function of the J / ψ transverse momentum in the range 2 < p T J / ψ < 15 GeV / c. The results are in excellent agreement with next-to-leading order non-relativistic expectations and show a significant discrepancy compared with the colour singlet model prediction at leading order, especially in the low p T J / ψ region
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