36 research outputs found
Relative Expression of CMyc MRNA in Human Glioma Cells and Its Relationship with the Degree of Malignancy
Full text linkRelative Expression of cMyc85Vol. 5, No. 2, Agustus 2017RESEARCH ARTICLERelative Expression of cMyc mRNA in Human Glioma Cells and its Relationship with the Degree of Malignancy Fidinny I. Hamid,1 Novi S. Hardiany,2* Febrial Hikmah,3 Syaiful Ichwan41Undergraduate Program FM Universitas Indonesia2Department of Biochemistry and Molecular Biology, FM Universitas Indonesia3Biomedical Sciences Program FM Universitas Indonesia4Department of Neurosurgery FM Universitas Indonesia -Dr Cipto Mangunkusumo General Hospital*Corresponding author: [email protected] 10 April 2016; Accepted 23 Agustus 2017DOI: 10.23886/ejki.5.7467AbstractPluripotency of cMyc genes may be one factor for the high glial cell differentiation in glioma thus it can become an alternative therapeutic target. The objective of the study is toanalyze the cMyc mRNA expression and its relationship with the degrees of glioma Malignancy. This is a cross-sectional study from 20 glioma samples with different degree of Malignancy from Dr. Cipto Mangunkusumo General Hospital, Jakarta during the period of October 2014 until February 2015. The mRNA obtained from glioma samples are converted to cDNA and then amplified. Relative quantification of cMyc mRNA expression is measured by calculating the cycle threshold values of Real Time RT PCR and normalized towards 18s rRNA to predict the relationship between the expression of cMyc and the degree of Malignancy. The cMyc expression is increased in accordance with the tumor grade. The cMyc expressions in high grade glioma are 17424.23 folds higher when calibrated to the normal cell, whereas the genes in lower grade tumors are expressed with the rate of 6167.35. Although the values obtained are not statistically significant, this research has strengthened molecular diagnosis, specifically pluripotency as the factor that gives a greater prognostic relevance than the histopathologic diagnosis. As a conclusion, there is a clinical tendency where the relative mRNA expression was higher in in glioma cMyc high degrees compared with low-grade glioma, however it is not statistically significant
Production of recombinant protein by gene cloning from mammalian cell culture
No full textCancer is defined as a group of neoplastic diseases broadly characterized by abnormalities of cellular differentiation, maturation and control of growth. Cancer significantly contributes to the bulk of deaths globally. It is the number two killer,after cardiovascular disease.The p53 tumor suppressor protein plays a central role in the cellular response to DNA damage by controlling genes involved in cell cycle arrest and apoptosis. The
mutation of p53 is one of the most common events found in human cancers. When p53 is lost of its function this may permit the cell to divide without control.
The purpose of the research is to produce recombinant protein from cancer cells harboring a specific mutation of the p53 gene. In the future, the recombinant protein can be use to prevent or to treat the cancer as a part of gene therapy
PERCUTANEOUS EPIDURAL NEUROPLASTY (PEN) USING COMBINATION OF HYALURONIDASE AND HYPERTONIC SALINE (NaCl 3%) IN TREATING FAILED BACK SURGERY SYNDROME
No full textBackground: Following surgical treatments for low back pain, lower extremity pain or neurologic symptoms would last or recur, this is defined as failed sack surgery syndrome (FBSS). FBSS usually occurs in 5-40% of these surgical patients. The most common cause is an epidural scar adhesion. Percutaneous epidural neuroplasty is the non-mechanical treatment for this condition. Previously, the use of hyaluronidase and hypertonic saline separately is commonly used for epidurolysis but the combination of hyaluronidase and hypertonic saline 3% has not been explored.Objective: To investigate the two-year outcomes of percutaneous epidural neuroplasty using a combination of hyaluronidase and hypertonic saline 3% in patients with FBSS.Methods: Twelve patients who experience low back pain, with or without radiculopathy, who have underwent lumbar spine surgery previously were assigned to the study. Parameters, such as the visual analogue scale scores for the back (VAS-B) and legs (VAS-L), and the Oswestry disability index (ODI), were recorded and compared between pretreatment, 1 week, 1 month, 3 months, 1 year and 2 years follow-up.Results: For all 12 patients, the postoperative VAS-B, VAS-L, and ODI were significantly different from the preoperative values in all follow-up periods: 1 month, 3 months, 1 year, and 2 years.Conclusion: Based off this study group, percutaneous epidural neuroplasty using a combination of hyaluronidase and hypertonic saline 3% has a favourable outcome in the 2 years follow-u
KINERJA PEGAWAI DALAM PELAYANAN PUBLIK DI KELURAHAN MARIYAI KABUPATEN SORONG
Full text linkKinerja Pegawai Dalam Pelayanan Publik Di Kelurahan Mariyai Kabupaten Sorong. Penelitian ini bertujuan untuk mengetahui Bagaimana kinerja pegawai dalam pelayanan publik di Kelurahan Mariyai Kabupaten Sorong, untuk mengetahui faktor penghambat yang mempengaruhi kinerja pegawai dalam pelayanan publik di Kelurahan Mariyai, untuk mengetahui apa saja upaya kinerja pegawai di Kelurahan Mariyai. Metode Penelitian yang digunakan adalah penelitian kualitatif dengan tipe penelitian yang bersifat deskriptif kualitatif dan menggunakan informan penelitian, dan juga menggunakan teknik pengumpulan data yang digunakan yaitu observasi, wawancara dan dokumentasi. Hasil Penelitian di lapangan membuktikan bahwa kinerja pegawai pada Kelurahan Mariyai sudah begitu baik dilhat dari pelayanan yang dilakukan oleh pegawai kepada masyarakat dalam proses pembuatan surat domisili tanpa menunda-nunda waktu
GOVERNING HAJJ: Politics of Islamic Pilgrimage Services in Indonesia Prior to Reformasi Era
Full text linkThis article highlights that the hajj (Islamic pilgrimage to Mecca) involves not only religious devotion, but also religious tourism and its associated business, necessary to deal with massive parties of pilgrims, embracing trans-national relations, central and local governments, flight and other travel agencies, pilgrimage guidance units, catering agencies and hotels to the pilgrims themselves in its scope. The aim of this article is to analyse the politics of hajj services, which was carried out mainly through the placing of this pilgrimage under government control, leading to the assumption of its monopoly by the government during the New Order period. Although it will focus on Soeharto period, there will be some discussions on this subject during the colonial and early post-colonial periods to trace the genealogy of government control of hajj pilgrimage (and ‘umrah, known also as ‘small hajj’), especially during the New Order. The author argues that the complexities of hajj (and ‘umrah) services were not so much caused by religious aspect but rather by political and economic motives.</p
Surgery of Left Temporal Region Arachnoid Cyst with Neuroendoscopy: A Case Report
Full text linkIntroduction: Today, the development of minimally invasive neurosurgery technique, has become a choice of treatment for many neurosurgical disease. Dr.Suyoto Hospital, Rehabilitation Center, Ministry of Defence of the Republic of Indonesia and Indonesian Airforce Hospital Dr. Esnawan Antariksa, Halim Perdanakusuma, Jakarta, Indonesia, has responsibility in public health services for military and civilian community. This paper has an objective to share experience in giving treatment with intracranial neuroendoscopy technique for patient with left temporal region arachnoid cyst.
Case Report: Case Report 1 : Girl, 17 years old, with headache. There was no neurological deficit, and from brain CT Scan, there was a cystic lesion at the left temporal region. The diagnosis was arachnoid cyst. She performed neuroendoscopic cystotomy and insertion of Omaya reservoir. After surgery, she had no headache, and there were no post-operative complications. Histopatology finding was arachnoid cyst. From follow up of brain CT Scan, there was improvement. We used intracranial neuroendoscopy device from B-Braun Aesculap, Germany, 2015. Case Report 2 : Boy, 8 years old, with seizure and headache. There was no neurological deficit, and from brain CT Scan, there was a cystic lesion at the left temporal region. The diagnosis was arachnoid cyst. He performed neuroendoscopic cystotomy and insertion of Omaya reservoir.
Dicussion: After surgery, he had no headache and also had no seizure, and there were no post-operative complications. Histopatology finding was arachnoid cyst. From follow up of brain CT Scan, there was improvement. We used intracranial neuroendoscopy device from B-Braun Aesculap, Germany, 2015.
Conclusion: Intracranial neuroendoscopy technique can be applied for the treatment of many special and selective neurosurgical diseases, including arachnoid cyst. In this patient, intracranial neuroendoscopy had good result. We still need more many of cases for determine the success rate of this intracranial neuroendoscopy technique statisticall
Relative Expression of cMyc mRNA in Human Glioma Cells and its Relationship with the Degree of Malignancy
No full textRelative Expression of cMyc85Vol. 5, No. 2, Agustus 2017RESEARCH ARTICLERelative Expression of cMyc mRNA in Human Glioma Cells and its Relationship with the Degree of Malignancy Fidinny I. Hamid,1 Novi S. Hardiany,2* Febrial Hikmah,3 Syaiful Ichwan41Undergraduate Program FM Universitas Indonesia2Department of Biochemistry and Molecular Biology, FM Universitas Indonesia3Biomedical Sciences Program FM Universitas Indonesia4Department of Neurosurgery FM Universitas Indonesia -Dr Cipto Mangunkusumo General Hospital*Corresponding author: [email protected] 10 April 2016; Accepted 23 Agustus 2017DOI: 10.23886/ejki.5.7467AbstractPluripotency of cMyc genes may be one factor for the high glial cell differentiation in glioma thus it can become an alternative therapeutic target. The objective of the study is toanalyze the cMyc mRNA expression and its relationship with the degrees of glioma malignancy. This is a cross-sectional study from 20 glioma samples with different degree of malignancy from Dr. Cipto Mangunkusumo General Hospital, Jakarta during the period of October 2014 until February 2015. The mRNA obtained from glioma samples are converted to cDNA and then amplified. Relative quantification of cMyc mRNA expression is measured by calculating the cycle threshold values of Real Time RT PCR and normalized towards 18s rRNA to predict the relationship between the expression of cMyc and the degree of malignancy. The cMyc expression is increased in accordance with the tumor grade. The cMyc expressions in high grade glioma are 17424.23 folds higher when calibrated to the normal cell, whereas the genes in lower grade tumors are expressed with the rate of 6167.35. Although the values obtained are not statistically significant, this research has strengthened molecular diagnosis, specifically pluripotency as the factor that gives a greater prognostic relevance than the histopathologic diagnosis. As a conclusion, there is a clinical tendency where the relative mRNA expression was higher in in glioma cMyc high degrees compared with low-grade glioma, however it is not statistically significant
Relasi Politik Islam Terkait Islam Aceh dan Islam Turki
Full text linkThis article seeks to explain Islamic thought related to the relationship between Acehnese Islam and Turkish Islam both through political and religious sectors that the author adopted as more dominant. The relationship between the two was harmonious even though it was said that Aceh and Turkey had such a long distance, so the argument about the harmonious relationship was so evocative of the treasure of thought to be followed up. This study aims to find out how the history of Thought relates to the Islamic Relations of Aceh and Turkey in Islamic politics. The method used in the study is a literature review (library research). So in this study the authors found that in the political field when the Kingdom of Aceh was in the Dutch colonial hegemony barrier, the kingdom of Turkey helped Aceh both from helping logistics related to military weaponry and even participated in sending troops to Aceh. Then, on the other side of the relationship with religion, the author found related to the jihad of the Acehnese people as a spirited and very tempestuous spirit naya in the Aceh war which is often told through the saga of the sabil war
DISKURSUS TENTANG PERLU TIDAKNYA POLITIK HUKUM PASAR MODAL SYARIAH DI INDONESIA
Full text linkThis study is motivated by the existence of the capital market into a reality and the latest phenomenon in the midst of the lives of Muslims in this modern century, even almost countries around the world have used the capital market as an important economic instrument to invest. In 2003, the Capital Market Supervisory Agency (Bapepam) launched the Islamic capital market. The presence of the Islamic capital market needs to be supported by stronger regulations in the form of a law. This article uses library data or library research with juridical-normative type. n this paper, the author states that the politics of law is a legal policy or official policy line regarding the law that will be enforced either in terms of making new laws or replacing old laws in the process of achieving state goals. The idea of supporting the existence of Islamic capital markets has at least four reasons behind it, namely: First, there is a belief that abundant wealth if not invested in the right place will be wasted. Second, fuqaha and Islamic economists have been able to create Islamic-based securities as an alternative to securities in circulation that are not in accordance with Islamic law. Third, it protects Muslim rulers and businessmen from speculators when investing or financing securities. Fourth, it provides a place for Islamic financial institutions and sciences related to trading techniques, while conducting sharia-compliant activities. Keywords: Political Law, Capital Market, Sharia
SINTESIS TENTANG INTERAKSI HUKUM ISLAM DAN HUKUM ADAT PADA MASA KOLONIAL BELANDA
Full text linkThis article is motivated by the Dutch legal political policy, which is known as the bamboo splitting policy which brings together face to face between customary law, Islamic law and Western law. The Dutch East Indies policy received a response from Muslims, because the Muslim community was actually in direct contact with practices as religious activities in the implementation of their teachings. This article uses a qualitative descriptive approach which is more specifically on historical aspects and uses bibliographic data sources and the type of data is secondary data. In this paper the author results that the interaction of Islamic law with customary law is described in a variety of forms. First, customary law can substantially be accepted by Islamic law as a whole, so that in the future it becomes Islamic law. Second, Islamic law has completely changed customary law, so that the validity of customary law does not apply to the next. Third, Islamic law allows customary law to apply without any effort to inhale it, this is sometimes found in the field of muamalah.Keywords: Dutch East Indies, Islamic Law, Customary Law
