6 research outputs found
Unexpected ventricular fibrillation triggered by pulsed field ablation for atrial fibrillation
No full textDigital Desire and Recorded Music: OiNK, Mnemotechnics and the Private BitTorrent Architecture
No full textThis thesis centres on the P2P internet protocol BitTorrent, music filesharing, and nascent forms of collective action developing through private BitTorrent communities. The focus is on one of these communities, a music filesharing website called ‘OiNK’. Founded in 2005, it was the first of its kind to garner membership in the hundreds of thousands, was emblematic of user-led movements to improve the quality, efficiency and availability of digital media online, and was very publically shut down in 2007.
Making critical use of Simondon’s notion of ‘individuation’, two interrelated techno-historical impulses are identified as central to the ‘in-formation’ of both BitTorrent and OiNK. Firstly, through research into the development of the global music industry’s ‘productive circuit’ of manufacturing, distribution, retail and radio, it is shown how consumers were gradually excluded from having a say in how, what and where they could consume. Secondly, a history of ‘OiNK-style’ filesharing is gleaned, not from P2P, but from research into small, decentralised ‘online’ communities that emerged throughout the 1980’s and 1990’s, where enthusiasts learned how to use software and hardware to manage the storage, reproduction, uploading and sharing of information.
This thesis shows how BitTorrent exposed these previously exclusive practices to masses of consumers who were dissatisfied with both retail/broadcasting and public P2P, through the new possibility of private BitTorrent communities. Through a case study of OiNK, encompassing in-depth interviews with ex-members, screenshots and technical analysis, this thesis shows how OiNK gathered a large and diverse online cache of ‘archival grade’ recorded music, bringing old and deleted music back into circulation and amassing a core of fanatically committed members. It accounts for a nascent form of online community, where large member-bases manage every aspect of the reproduction and circulation of digital artefacts, and at higher levels of quality/efficiency than legal alternatives
Correction: Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19
No full textInternational audienc
Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19
No full textBackgroundWe previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent type I interferon (IFN) immunity or to autoantibodies against type I IFN, account for 15-20% of cases of life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants of life-threatening COVID-19 remain to be identified in similar to 80% of cases.MethodsWe report here a genome-wide rare variant burden association analysis in 3269 unvaccinated patients with life-threatening COVID-19, and 1373 unvaccinated SARS-CoV-2-infected individuals without pneumonia. Among the 928 patients tested for autoantibodies against type I IFN, a quarter (234) were positive and were excluded.ResultsNo gene reached genome-wide significance. Under a recessive model, the most significant gene with at-risk variants was TLR7, with an OR of 27.68 (95%CI 1.5-528.7, P=1.1x10(-4)) for biochemically loss-of-function (bLOF) variants. We replicated the enrichment in rare predicted LOF (pLOF) variants at 13 influenza susceptibility loci involved in TLR3-dependent type I IFN immunity (OR=3.70[95%CI 1.3-8.2], P=2.1x10(-4)). This enrichment was further strengthened by (1) adding the recently reported TYK2 and TLR7 COVID-19 loci, particularly under a recessive model (OR=19.65[95%CI 2.1-2635.4], P=3.4x10(-3)), and (2) considering as pLOF branchpoint variants with potentially strong impacts on splicing among the 15 loci (OR=4.40[9%CI 2.3-8.4], P=7.7x10(-8)). Finally, the patients with pLOF/bLOF variants at these 15 loci were significantly younger (mean age [SD]=43.3 [20.3] years) than the other patients (56.0 [17.3] years; P=1.68x10(-5)).ConclusionsRare variants of TLR3- and TLR7-dependent type I IFN immunity genes can underlie life-threatening COVID-19, particularly with recessive inheritance, in patients under 60 years old
Enfermedad mixomatosa de la válvula mitral; manejo clínico y plan terapéutico del paciente canino
No full textLa Enfermedad mixomatosa de la válvula mitral es la patología cardiaca más importante en la medicina cardiovascular de pequeños animales, particularmente en los perros. Debido a que la MMVD es un ejemplo de una enfermedad crónica que progresa de leve a grave a lo largo de los años, las estrategias de tratamiento cambian con la etapa de la enfermedad. El manejo exitoso de la enfermedad cardíaca requiere terapia oportuna y multimodal, con el objetivo de mantener la calidad de vida para el paciente y el propietario.Myxomatous mitral valve disease is the most important cardiac pathology in cardiovascular medicine of small animals, particularly in dogs. Because MMVD is an example of a chronic disease that progresses from mild to severe over the years, treatment strategies change with the stage of the disease. The successful management of heart disease requires timely and multimodal therapy, with the aim of maintaining the quality of life for the patient and the owner.Resumen. --
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Realizar una búsqueda sistemática de literatura sobre los planes terapéuticos y el manejo clínico de la Enfermedad de la válvula mitral mixomatosa en perros. --
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Cronograma de [email protected]
Analysis of biological relationships in the hemoparasitechelonian model in Colombia
No full textilustraciones, fotografías, gráficas, mapas, tablasAlgunos géneros de parásitos del suborden Adeleorina infectan usualmente hospederos intermediarios como las tortugas. Sin embargo, el conocimiento que se tiene acerca de las especies de parásitos que infectan estos hospederos es escasa. En la literatura, para la identificación de parásitos de este suborden se usan herramientas morfológicas, morfométricas y técnicas moleculares; principalmente información del gen 18S rDNA. Sin embargo, el uso de este gen tiene varias deficiencias. Para incrementar el conocimiento de los parásitos que infectan quelonios, así como en las técnicas moleculares para investigación en diversidad, este estudio tuvo como objetivos: 1) identificar la diversidad genética de linajes de parásitos aislados mediante amplificación distintos genes, 2) Integrar líneas de evidencia tanto morfológica como molecular para la identificación de unidades linajes evolutivos desconocidos y 3) diseñar herramientas alternativas para el diagnóstico e identificación de estos parásitos mediante la implementación de nuevos marcadores moleculares que han sido poco explorados en organismos de este suborden.
Para alcanzar estos objetivos se obtuvieron y analizaron muestras sanguíneas de 457 individuos pertenecientes a 16 de las 29 especies de tortugas continentales presentes en Colombia. Todas las muestras fueron analizadas por métodos moleculares y 203 de estas muestras también fueron analizadas por microscopia. Como resultado de los analisis se pudo describir una nueva especie de Hepatozoon (Hepatozoon simidi sp. nov) en Rhinoclemmys melanosterna (Geoemyidide) basados en información acerca de la morfología de los estadios sanguíneos y secuencias de un fragmento del gen 18S. También se diseñaron nuevos sets de primers para los genes mitocondriales citocromo oxidasa I (coxI), citocromo oxidasa III (coxIII) y citocromo b (cytb) basados en la secuenciación genómica del parásito obtenido de una muestra tomada de Podocnemis vogli (Podocnmeididae). Se aislaron por lo menos 30 linajes diferentes de cada gen analizado, demostrando una alta diversidad genética de parásitos en estos hospederos. Sin embargo, los índices de diversidad evaluados demuestran que aún se desconoce la diversidad real de linajes parasitarios en estos hospederos. Adicionalmente, los resultados también evidenciaron varios procesos como heteroplasmía y/o especiación críptica, están afectando la identificación y caracterización de la diversidad de parásitos. Este estudio constituye el comienzo de una interesante e importante área de investigación. (Texto tomado de la fuente)Some genera of parasites of the suborder Adeleorina usually infect intermediate hosts such as turtles. However, little is known about the diversity of parasites that infect these hosts. In the literature, for the identification of parasites of this suborder, morphological and morphometric tools as well as molecular techniques are used; mainly information from the 18S rDNA gene. However, the use of this gene has several shortfalls. To increase the knowledge on parasites infecting chelonians and the knowledge on different molecular markers that can offer more inflrmation for diversity reseach, this study had the following goals: 1) to identify the genetic diversity of isolated parasite lineages using the amplification of different genes, 2) to integrate morphological and molecular evidence for the identification of unknown evolutionary lineages, and 3) to design alternative tools for the diagnosis and identification of these parasites through the implementation of new molecular markers that have been little explored in organisms of this suborder and that are infecting turtles in Colombia. To reach those goals, a total of 457 blood smples of individuals belonging to 16 of the 29 species of continental turtles in Colombia were obtained and analyzed. All samples were analyzed by molecular methods and 203 of these samples were also analyzed by microscopy. As results of the performed analyses, a new species of Hepatozoon (Hepatozoon simidi sp. nov) was described infecting Rhinoclemmys melanosterna (Geoemydidae) based on the integration of morphological information of the blood stages and the sequences of a fragment of the 18S gene. New sets of primers for the mitochondrial genes cytochrome oxidase I (coxI), cytochrome oxidase III (coxIII) and cytochrome b (cytb) were designed based on the genomic sequencing of a sample from a parasite that had infected a Podocnemis vogli (Podocnemididae) individual. At least 30 different lineages were isolated from each gene analyzed, evidencing a high genetic diversity of parasites in these hosts. However, diversity indexes evaluated showed that the real diversity of parasitic lineages in these hosts is still unknown. Aditionally, the results also evidenced several phenomena such as heteroplasmy and /or criptic speciation, which are affecting the identification and characterization of the diversity of parasites. This study represents the beggining of an interesting and important field of research.MaestríaMagister en Infecciones y salud en el TrópicoParasitología y Biología molecular en reptile
