39 research outputs found
Pemurnian Sistem Selulase dari Bacillus Subtilis dengan Metode Fraksinasi Ammonium Sulfat Fraksi 0-20%, 20-40%, 40-60%, 60-80%, dan 80-100%.
ABSTRAK Afrizal, H. 2012. Pemurnian Sistem Selulase dari Bacillus Subtilis dengan Metode Fraksinasi Ammonium Sulfat Fraksi 0-20%, 20-40%, 40-60%, 60-80%, dan 80-100%. Skripsi, Jurusan Kimia, FMIPA, Universitas Negeri Malang. Pembimbing: (I) Evi Susanti, S. Si., M. Si., (II) Muntholib, S. Pd., M. Si. Kata kunci : sistem selulase, Bacillus subtilis, pemurnian enzim, fraksinasi ammonium sulfat Penggunaan ekstrak kasar selulase pada dunia industri kurang diminati karena efektivitas dan stabilitasnya rendah. Hal ini karena ekstrak kasar umumnya mengandung protease yang dapat mendegradasi selulase. Oleh karena itu, perlu dilakukan pemurnian untuk meningkatkan aktivitas dan stabilitas enzim selulase. Tujuan dari penelitian ini adalah untuk mengetahui (1) aktivitas spesifik, tingkat kemurnian, dan yield sistem selulase meliputi aktivitas CMCase dan Avicelase, yang dihasilkan dari pemurnian sistem selulase dengan metode fraksinasi ammonium sulfat fraksi 0-20%, 20-40%, 40-60%, 60-80%, dan 80-100%; dan (2) stabilitas aktivitas CMCase dan Avicelase pada fraksi dengan aktivitas spesifik tertinggi selama penyimpanan pada suhu 4oC . Penelitian ini bersifat laboratoris dan terdiri dari beberapa tahap yaitu (1) pro-duksi ekstrak kasar sistem selulase dari Bacillus subtilis; (2) penentuan aktivitas spesifik ekstrak kasar sistem selulase; (3) pemurnian enzim dengan metode fraksinasi ammonium sulfat; (4) penentuan aktivitas spesifik, tingkat kemurnian, dan yield fraksi 0-20%, 20-40%, 40-60%, 60-80%, dan 80-100%; dan (5) penentuan stabilitas aktivitas sistem selulase pada fraksi dengan aktivitas spesifik tertinggi selama penyimpanan pada suhu 4oC. Aktivitas Avicelase dan CMCase diukur berdasarkan ujung pereduksi yang dihasilkan setelah reaksi dengan menggunakan metode Somogyi-Nelson. Kadar protein ditentukan dengan metode Lowry. Tingkat kemurnian ditentukan dari perbandingan aktivitas spesifik enzim setelah dimurnikan dengan ekstrak kasar. Yield enzim merupakan perbandingan dari aktivitas total enzim setelah dimurnikan dengan aktivitas total ekstrak kasar dikali 100%. Hasil penelitian menunjukkan bahwa fraksi 20-40% merupakan fraksi dengan aktivitas spesifik, tingkat kemurnian, dan yield tertinggi berturut-turut sebesar 3.065,64 U/mg, 4,28 kali ekstrak kasar, dan 13,72% untuk aktivitas CMCase dan 3.007,29 U/mg, 3,14 kali ekstrak kasar, dan 16,05% untuk aktivitas Avicelase. Stabilitas aktivitas CMCase maupun Avicelase pada fraksi 20-40% selama penyimpanan pada suhu 4oC mengalami penurunan mulai hari pertama hingga tidak memiliki aktivitas pada hari ke-9.
Gaya Bahasa Dalam Kepengarangan Puisi: Kajian Perbandingan Stilistik Penerima S.E.A Write Award Malaysia (Usman Awang) Dan Indonesia (Afrizal Malna): Language Styles In Poetry Authors: A Stylistic Comparison Study Of Recipients S.E.A Write Award Malaysia (Usman Awang) And Indonesia (Afrizal Malna)
Teknik penulisan dalam sesebuah karya sastera adalah cerminan dari legasi pemikiran dan keperibadian seseorang penulis. Pengungkapan melalui tema, persoalan dan langgam bahasa yang dihasilkan penulis meletakkan diri mereka dalam kategori yang tertentu. Puisi merupakan antara genre dianggap sukar untuk dimergertikan dari aspek penggayaan bahasa yang digunakan. Diksi yang minima tetapi sarat dengan perjuangan falsafah, menuntut pembaca untuk mentafsir dari pelbagai sudut persepsi. Tokoh penyair Malaysia seperti Usman Awang, A. Samad Ismail, dan Baha Zain antara penyair yang mempunyai identiti tersendiri dalam mewadahkan bahasa kreatif mereka. Begitu juga dengan negara Indonesia seperti Sapardi Djoko Damono, Chairil Anwar, dan Afrizal Malna turut mempunyai kredibiliti tersendiri dalam teknik penulisan puisi. Justeru itu, kajian ini dijalankan sebagai merungkai persoalan tentang proses penulisan sajak dari aspek teknik gaya bahasa yang disampaikan penyair. Pemilihan dua tokoh sastera nusantara iaitu Usman Awang dan Afrizal Malna yang juga penerima Anugerah S.E.A Write terawal dan mutakhir dalam skop kajian adalah bagi memperlihat identiti gaya bahasa yang diguna pakai mereka ketika proses pengkaryaan dijalankan. Kedua-dua tokoh ini begitu terkenal dengan aliran perjuangan kemanusiaan yang menjadikan penulisan sajak sebagai medan menyedarkan sikap kemanusiaan. Meskipun begitu, mereka mempunyai cara tersendiri dalam penyampaian karya. Kaedah kajian ini dijalankan secara kualitatif dengan menganalisis dokumen teks. Teori stilistik dijadikan sebagai sandaran untuk merungkaikan setiap teknik gaya bahasa, unsur kesamaran, bauran kiasan dan simbolisme bagi membuktikan falsafah gaya penyampaian pengkarya. Hasil kajian perbandingan ini mendapati bahawa kedua-dua tokoh penyair ini mempunyai keunikan tersendiri dalam menyempurnakan tanggungjawab sebagai pejuang kemanusiaan. Rentak implisit, satira dan simbolisme yang digarapkan tokoh penyair Malaysia dan Indonesia ini adalah cerminan terhadap nilai falsafah keintelektualan mereka berkarya, di samping memberi kesedaran kepada masyarakat terhadap keunggulan sastera dalam memperjuangkan hak kemanusiaan yang tertindas.
ABSTRACT
The writing technique in a literary work is a reflection of the legacy of a writer\u27s thoughts and personality. Expressions through themes, questions and language expressions produced by the author place themselves in a certain category. Poetry between genres is considered difficult to understand from the aspect of language styling used. Minimal but full of philosophical struggles requires the reader to interpret from various angles of perception. Malaysian poets such as Usman Awang, A. Samad Ismail, and Baha Zain are among the poets who have their own identity in conveying their creative language. Similarly, the Indonesian nation such as Sapardi Djoko Damono, Chairil Anwar, and Afrizal Malna also have their own credibility in poetry writing techniques. Therefore, this study was conducted to unravel the question of the process of writing poems from the aspect of language style techniques presented by poets. The selection of two nusantara literary figures, namely Usman Awang and Afrizal Malna who are also the recipients of the earliest and most recent S.E.A Write Award in the scope of the study is to show the identity of the language style used by them during the creative process. These two figures are so famous for the flow of humanitarian struggles that make poetry writing a platform to awaken the humanitarian attitude. Even so, they have their own way of delivering work. The method of this study was conducted qualitatively by analyzing the text documents. Stylistic theory is used as a basis to unravel every technique of language style, elements of ambiguity, fusion mixing and symbolism to prove the philosophy of the artist\u27s delivery style. The results of this comparative study found that the two poets have their own uniqueness in fulfilling their responsibilities as humanitarian fighters. The implicit rhythm, satire and symbolism employed by Malaysian and Indonesian poets are a reflection of the philosophical value of their intellectualism in their work, while giving awareness to the community on the superiority of literature in fighting for oppressed human rights
Analisis Nilai Perjuangan Sosial Tokoh Utama dalam Novel Hang Perkasa Karya Afrizal Cik
This research aims to analyze the intrinsic elements and values of social struggle
contained in the novel Hang Perkasa by Afrizal Cik. The main topic of discussion in
this research is how the structural study of the novel Hang Perkasa includes themes,
characters and characterization, plot, setting and point of view as well as the value of
struggle contained in the novel. The approach used in analyzing this study is a structural
study and literary sociology by looking at the sociological essence of the work. This
research uses a descriptive qualitative method that focuses on novel texts as primary
data sources and other research reports as secondary sources. The text symptoms
reviewed are related to what the main character does for the common good in family
and social life towards wise values and are based on Malay philosophy. The results of
this research describe the structure of the novel Hang Perkasa as follows: the main
character is played by Hang Perkasa eyebrows Panglima Hitam. There are five
protagonists and one antagonist. The plot used by the author in the novel Hang Perkasa
is a progressive plot with the peak of the story's conflict occurring when the main
character is slandered by the antagonist and then imprisoned. Setting: Pulau Manggung
Village, Kuala Malaka, Bandar Malaka, Tanjung Jati, Tanjung Pulau Merbau or
Kampung Centai. Time setting: end of the month of Safar, tenth day, and one night.
Social setting: place of study, palace circles, villagers or Kuala Panggung village. The
point of view contained in the novel Hang Perkasa is an omniscient third person
perspective which is represented by the author through a character named Hang
Perkasa, the mighty Black Panglima Ali eyebrows. In fighting for social values in
social life from when we were young to having a family. The values of this struggle
are summarized in willingness to sacrifice, unity, respect, patience and a spirit of never
giving up, as well as good cooperation.90 HalamanSkripsi Sarjan
Strategi Pemenuhan Pemeriksa di Lembaga Internasional pada Badan Pemeriksa Keuangan
This research aims to analyze the factors that are considered in the selection of auditors in carrying out audit assignments in international institutions and formulate strategies for the fulfillment of employees for audits in international institutions at The Audit Board of The Republic of Indonesia. The research was carried out at the Audit Board of the Republic of Indonesia at the Human Resources Bureau. This research is intended to formulate more effective and efficient policies in human resource management, especially the management of auditors in The Audit Board of The Republic of Indonesia.
The author uses several theories related to public administration, human resource management, employee placement, and management strategies obtained through the opinions of experts and previous research. This study uses a qualitative descriptive method. The informants in this study are the parties involved in the recruitment and placement process of employees at the Financial Audit Agency consisting of HR managers and parties involved in audits at international institutions carried out by the The Audit Board of The Republic of Indonesia. The data sources consist of primary data and those collected through semi-structured interviews and observations. In addition, the author also needs secondary data obtained through analysis of documents and applicable rules, especially related to recruitment placement and examiner placement. Data analysis uses reduction analysis, data display and conclusions or conclusions
Paradigma Baru Birokrasi Pemerinthan dalam Mewujudkan Tata Kelola Pemerintahan yang Baik
Substitution of the new order regime reform era to bring home big enough task for holders of development policy in this country . Government bureaucracy into one part left by the new order of special concern by the government , especially pemerintaha area. The inability of the government to manage the bureaucracy caused by many things one of which is the lack of quality of personnel in providing service to the community . Apparatus still positioning itself not as a public servant but someone who must be served . Therefore, in this paper the author tries to analyze the problems with the approach of a paradigm shift regarding the bureaucratic apparatus of government as part of efforts to resolve the issue of government bureaucracy in order to realize good governance
Paradigma Baru Birokrasi Pemerinthan dalam Mewujudkan Tata Kelola Pemerintahan yang Baik
Substitution of the new order regime reform era to bring home big enough task for holders of development policy in this country . Government bureaucracy into one part left by the new order of special concern by the government , especially pemerintaha area. The inability of the government to manage the bureaucracy caused by many things one of which is the lack of quality of personnel in providing service to the community . Apparatus still positioning itself not as a public servant but someone who must be served . Therefore, in this paper the author tries to analyze the problems with the approach of a paradigm shift regarding the bureaucratic apparatus of government as part of efforts to resolve the issue of government bureaucracy in order to realize good governance
The impact of mathematical models of teaching materials on square and rectangle concepts to improve students’ mathematical connection ability and mathematical disposition in middle school
Upaya Indonesia dalam Penguatan Usaha Mikro Kecil Menengah untuk Mengahadapi ASEAN Economic Community 2015 (Studi Kasus: Industri Kecil Menengah Kerajinan Kayu Bali Tahun 2010-2014)
This research aims to provide more knowledge to students and public about the effort in conducting the Indonesian Government to strengthen the micro, small, and medium enterprises to confront the ASEAN Economic Community by 2015, particularly on small and medium industry of Balis wood crafts in Bali.The research method that used in this study is a qualitative method, which begins with collecting, arranging and enterprising data. This research is an explanatory that describes a problem, symptoms, policies, and actions. Based on the phenomenon that is used as the problems to be studied by the author. While the author uses theory of role and foreign policy, and globalization theory to answer the basic problems that the authors including in this writing. Level analysis that the author used in this research is nation-state.The 2020 acceleration of ASEAN Economic Community to be coming 2015 is one of the seriousness from the fellow countries of ASEAN to realize the economic cooperation in Southeast Asia. Indonesia will be confronted with the opportunity and defiance at once when the ASEAN free trade enforced. To abuse the opportunity, Indonesia makes efforts in Micro, Small, and Medium Enterprises reinforcement with increase competitiveness Micro, Small, and Medium Enterprises in Indonesia. Micro, Small, and Medium Enterprises is one of important sector in Indonesia that have to empowered, because it gave significant contribution in Indonesias economic matters. Indonesia has prepare many various of program and wisdom to increase the Indonesias Micro, Small, and Medium Enterprises and Small Medium Industry competitiveness to confront the ASEAN Economic Community by 2015.Key Word: ASEAN Economic Community 2015, Micro, Small, and Medium Enterprises, Small Medium Industry
EVALUASI PELAKSANAAN KEWAJIBAN KEPALA DESA SEBAGAI FASILITATOR DALAM MEMBERDAYAKAN MASYARAKAT DESA DI DESA LUBUK BENDAHARA TIMUR KECAMATAN ROKAN IV KOTO KABUPATEN ROKAN HULU
Government Regulation No. 72 Year 2005 on the village of Article 15 Paragraph 1 of the obligations of the village chief said that one of the aforementioned article on the letter n said that the Village Head has a duty to empower people and institutions in the village. The aim of the study is to examine the results of implementation of Obligations Village Head In Empowering Village community and to determine the factors inhibiting the Implementation of Obligations Village Head In Empowering villagers in the village of Lubuk Bendahara Timur , District Rokan IV Koto, Rokan Hulu, the author uses the theory of evaluation according to Ndraha with indicator of community empowerment, empowerment of civil society as a partner, the welfare of society. The type of research is quantitative descriptive as well as the study population is the village chief Lubuk Bendahara Timur , the Village , Institutional village, and heads of family are determined by census technique and purposive sampling, kind of collection of data used are primary data and secondary data, techniques in data collection using questionnaires, interviews and observations, then the data were analyzed descriptively using quantitative descriptive method based on the analysis of meaning Evaluation of obligation village chief in empowering Village Community in the village of Lubuk Bendahara Timur District of Rokan IV Koto Rokan Hulu assessed Enough to Succeed, It is expected that the Government the village of Lubuk Bendahara Timur to further improve community development activities village so that people-power and welfare level increased
Dampak Kebijakan Pelayanan Perizinan Terpadu Dan Penanaman Modal Terhadap Daya Tarik Investasi Asing Di Provinsi Riau Tahun 2013-2016
With the enactment of regional autonomy by the Indonesian government, the Riau provincial government established an institution that serves to minimize the process, the permit is always requested by the owners of capital / investors. The agency is the Integrated Licensing Service Agency and Capital Investment. This study aims to determine the policy of the institution, to know foreign investment in Riau province, to know the impact of the policy of the institution to foreign investment in Riau province. This research uses the perspectives of liberalism, this theory is pioneered by david ricardo & adam amith. The concept of this theory is based on the idea of market sovereignty in the economy. In this study the author uses the policy theory of Richard Snyder as a reference. The results of this study are positively impacting the economy in Riau province through incentive policies in the field of foreign investment including tax facilities and licensing facilities
