33 research outputs found
Valutazione della conta differenziale delle cellule del latte in relazione allo stato sanitario della mammella
No full textIn dairy herd management, somatic cell counting is largely used as an indicator of mastitis. Differential cell counts been indicated as a more accurate method for mastitis identification. Little is known, though, about the expected profile in healthy milk samples. This cross-sectional study explored the inflammatory profiles in milk from 2 dairy herds known to be affected by mammary gland infections from different Staphylococcus aureus strains, and 1 herd known to be free of contagious milk pathogens. Milk sampling was performed on 50 animals. Somatic cell counts and bacteriological examination were performed as described on NMC guidelines. Relative percents of lymphocytes (L), macrophages (M), polymorphonuclear cells (PMN); and their ratios were assessed through milk cytology. Results confirmed that herd A was free from contagious pathogens, herd B was positive for a coagulase negative strain of S. aureus, and herd C was positive for S. aureus. In all herds, healthy quarters as well as subclinically mastitic quarters were found. When cytology results were grouped by herd, the inflammatory profiles resulted markedly different. Our results suggest that milk immune response can vary from herd to herd. We do not know, though, if the differences are given to different genetic background, or from different stimulus from the different pathogen strains. Further studies must be done to determinate if a sole factor could be implicated, or if this differences are given to a combination of those factors
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Status and Future of Microwave Cavity Axion Searches
No full textWe review the status of an ongoing large-scale search for axions which may constitute the dark matter of our Milky Way halo. The experiment is based on the microwave cavity technique proposed by Sikivie, and marks a ''second-generation'' to the original experiments performed by the Rochester-Brookhaven-Fermilab collaboration, and the University of Florida group. Sensitivity to galactic asions has been achieved, at least for one important model coupling. A remarkable breakthrough in making near-quantum Limited dc SQUID amplifiers in the several hundred megahertz range has provided the enabling technology for a major upgrade of this effort. By improving the noise temperature by more than an order of magnitude, a much more sensitive search may be carried out, greatly improving the prospects for discovering the asion
超高合金よりタングステン及びタンタルの回収
No full textIn the process of the manufacture of Ultra Hard．Steel with the Powder Metallurgy Method，it is unavoidable to produuce the reluctant and abundant scraps，from which the expensive metals are not easily recovered． The author recovered tungsten and tantalum as oxides at the average rate of 97％ （tungsten oxide）and 9O％ （tantalum oxide）from the scrap pieces which consisted of tungesten carbide（8％），titanium carbide（6％），tantalum carbide（4％）and cobalt（l0％). The most important chemical treatments are to be experienced．in the next processes，the pieces are heated 800℃ for l.5−2 hours in the air，then they become powders of oxides not to be attainable with physical methods. Potassium hydroxide，five times as much，is added to the weighed sample，then fused−at 800℃ for about one time，and allowed to cool in the air. Next tme，potassium carbonate, five times as much，is added to the cooled one， and then fused atll00℃ thorougly．On this o㏄asion，the iron crucible is less corroded．For the chemical separation of tungsten and tantalum，the greatest nervous attention is necessary so as not to fail to catch either of them completely
Metafora w dyskursie politycznym
No full textMetaphor is not infrequently madę use of in political discourse as a suggestive means of expressing a meaning and communicating assessments, that is, as an instrument of persu- asion rendering it possible to shape the recipients’ views, opinions and behaviours from the standpoint of interests of a specified political party or social group. This role is best served by metaphors with a simple syntactic structure, highly conventionalised and em- ploying stereotypical content in their vehicle. In her description of metaphor’s me- aning-creating mechanism, the author emphasises the particular status of metaphoric pre- dicate that is suggested by the utterance’s sender and independently produced by the rece- iver using the metaphor vehicle’s connotation. The valuing so expressed is not subject to direct negation and so is quite powerful in terms of persuasiveness. Use of metaphors car- rying different valuing connotations whilst originating from a single notional field, as well as inventive witty transformation of the metaphor carrier practised in order to divert one’s attention from the evaluating content transferred by the same, can serve to neutrali- se an assessment being suggested by a metaphor. Ludic operations, often carried out on metaphors, enhance by themselves the persuasive power of utterances madę by politi- cians
Metafora w dyskursie politycznym
No full textMetaphor is not infrequently madę use of in political discourse as a suggestive means of expressing a meaning and communicating assessments, that is, as an instrument of persu- asion rendering it possible to shape the recipients’ views, opinions and behaviours from the standpoint of interests of a specified political party or social group. This role is best served by metaphors with a simple syntactic structure, highly conventionalised and em- ploying stereotypical content in their vehicle. In her description of metaphor’s me- aning-creating mechanism, the author emphasises the particular status of metaphoric pre- dicate that is suggested by the utterance’s sender and independently produced by the rece- iver using the metaphor vehicle’s connotation. The valuing so expressed is not subject to direct negation and so is quite powerful in terms of persuasiveness. Use of metaphors car- rying different valuing connotations whilst originating from a single notional field, as well as inventive witty transformation of the metaphor carrier practised in order to divert one’s attention from the evaluating content transferred by the same, can serve to neutrali- se an assessment being suggested by a metaphor. Ludic operations, often carried out on metaphors, enhance by themselves the persuasive power of utterances madę by politi- cians
Erancangan Board Game “Trash Pack” Sebagai Media Pembelajaran Pada Anak 7 Hingga 12 Tahun Tentang Pengelolaan Sampah
No full textMeningk|atny|a juml|ah penduduk, tingk|at konsumsi m|asy|ar|ak|at, d|an
kem|aju|an teknologi |ak|an meningk|atk|an juml|ah timbul|an s|amp|ah. Menurut
d|at|a Sistem Inform|asi Pengelol|a|an S|amp|ah N|asion|al (SIPSN) Kementeri|an
Lingkung|an Hidup d|an Kehut|an|an (KLHK), Indonesi|a mengh|asilk|an 35,83
jut|a ton timbul|an s|amp|ah sep|anj|ang 2022. Volume timbul|an s|amp|ah
tersebut n|aik 21,7% dib|anding 2021, sek|aligus menj|adi level tertinggi d|al|am
emp|at t|ahun ter|akhir seperti terlih|at p|ad|a gr|afik. Sedangan pada tahun 2023
terdapat 17,4 juta ton timbulan sampah tahunan yang dihasilkan. Dari data
tersebut, sebanyak 11,6 juta ton atau 66,47% sampah telah terkelola. Yang
artinya Volume timbulan sampah turun sebanyak 51,41% dibanding 2022.
Keberlanjutan lingkungan menghadapi tantangan serius akibat
peningkatan jumlah sampah yang dapat terjadi kapan pun disetiap tahunnya.
Penting untuk memulai edukasi mengenai pengenalan sampah dan
pengelolahannya sejak masih anak- anak agar dapat memahami dampak
lingkungan dari tindakan mereka.
Kurangnya media pembelajaran yang spesifik dan menarik untuk anak�anak di umur 6-12 tahun tentang sampah saat ini membuat kurangnya
pengetahuan anak tentang sampah disekitar mereka. Oleh karena itu,
perancangan board game dapat dianggap sebagai solusi yang kreatif.
Board game dirancang menggunakan metode design thinking yang
memuat lima tahapan yaitu emphatize, define, prototype, dan test.
Pengumpulan data dilakukan dengan cara observasi, wawancara, studi
literatur, dan studi komparasi. Hasil dari perancangan board game diuji dengan
bertahap hingga menghasilkan board game yang tepat guna untuk anak-anak
dengan usia 7-12 tahun. Board game “Trash Pack” dapat menjadi solusi untuk
media pembelajaran anak 7-12 tahun tentang pengelolaan sampah,
diharapkan untuk peneliti selanjutnya dapat mengembangkan board game
versi digital yang dapat dimainkan kapan saja dan di mana saja
PENGEMBANGAN VIDEO BLOG UNTUK MATA KULIAH PRAKTIKUM KONSELING PROGRAM STUDI BIMBINGAN KONSELING FAKULTAS ILMU PENDIDIKAN
No full textPENGEMBANGAN VIDEO BLOG UNTUK MATA KULIAH PRAKTIKUM KONSELING PROGRAM STUDI BIMBINGAN KONSELING FAKULTAS ILMU PENDIDIKAN
Oleh:
Satriyo Anung Pribadi
05104244079
ABSTRAK
Penelitian in i b ertu ju an u ntuk mengh as ilkan su atu produk me dia p e mb elajar an keteramp ilan konseling yang dik emas dalam bentuk video blog yang berhubungan deng an internet bag i mah asisw a pr aktik u m ko nsel ing Prodi Bim bingan Konseling Jurusan Psikologi Pendidikan dan Bimbingan Faku lt as Ilmu Pendidikan Universitas Negeri Yogya kar ta.
Peng emb an gan ini d idasarkan pad a hasil analisis kebutuh an mah asiswa y ang
disimpulkan bahwa mahasiswa mengharapkan sebuah media pembelajaran baru dalam prak tiku m konseli n g. S uby ek pen elitian ini te rb ag i atas b eb er ap a suby ek kelo mp ok, y aitu seoran g ah li ma teri, dan s eo r ang ahli tek nolo gi infor masi, sert a suby ek uji coba y ait u mah asiw a prodi bim b ing an d an konseli n g y ang te lah me n e mp uh perkuliah an praktiku m kon seling y ang b erju ml ah 3 0 or ang . P enen tuan suby ek mah asiswa te rsebut dilakuk an d eng an teknik purposive sampling. Tekn ik an alis is d ata d ilakuk an deng an teknik d e skriptif. Data berupa hasil p enilaian me ng en ai k ual itas prod uk, masukan, tanggap an dan sar an un tuk p erb aik an p r odu k. D at a kualit atif has il p en elit ian di an alis is deng an tekn ik analisis is i.
Hasil p en elitian p eng e mbang an ad alah s ebu ah si mu lasi v ideo keter a mp ilan konseling y ang me nggu nak an me d ia b lo g y ang tela h disele s aik an d en g an melakuk an uji lap ang an op er asion a l. Dari hasil uji lap ang an opera sion al 69,76 % mah asiswa me ny atak an baik, sed an gkan 30,24 % me ny atakan kurang baik. D apat disim p ulk a n bahw a mu ltime d ia vi d eo blog dala m prakti k u m konsel ing din y atak an lay ak dengan perti mb a ng an hasil an ali sis k uan ti tat i f serta tah ap an y ang tel a h dilalui s esuai.
K ata kunci: pengembangan video blog, praktikum konseling
CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells
No full textThe chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells.This project was funded by Instituto de Salud Carlos III, grant numbers PI20CIII/00020, DTS18CIII/00005, Asociación Pablo Ugarte, grant numbers TRPV205/18; Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, grant numbers TVP333-19, TVP-1324/15; ASION, grant number TVP141/17. Enrique Fernández-Tabanera is supported by Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, Saint T. Cervera is supported by Asociación Pablo Ugarte and Raquel M. Melero is supported by a CIBERER contract.S
The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
No full textEwing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a specific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knockdown inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell lines.This research was funded by the Instituto de Salud Carlos III, grant numbers PI20CIII/00020, DTS18CIII/00005, PI16CIII/00026; Asociacion Pablo Ugarte, grant numbers TRPV205/18, TPI-M 1149/13; Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, grant numbers TVP333-19, TVP-1324/15; ASION, grant number TVP141/17. R.M.M-FdM is supported for a grant of the Spanish Center for Biomedical Network Research on Rare Diseases (CIBERER). The laboratory of T.G.P.G. is supported by the Barbara and Wilfried Mohr Foundation.S
EWS-FLI1 confers exquisite sensitivity to NAMPT inhibition in Ewing sarcoma cells
No full textEwing sarcoma (EwS) is the second most common bone cancer in children and adolescents with a high metastatic potential. EwS development is driven by a specific chromosomal translocation resulting in the generation of a chimeric EWS-ETS transcription factor, most frequently EWS-FLI1.Nicotinamide adenine dinucleotide (NAD) is a key metabolite of energy metabolism involved in cellular redox reactions, DNA repair, and in the maintenance of genomic stability. This study describes targeting nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme of NAD synthesis, by FK866 in EwS cells. Here we report that blocking NAMPT leads to exhaustive NAD depletion in EwS cells, followed by a metabolic collapse and cell death. Using conditional EWS-FLI1 knockdown by doxycycline-inducible shRNA revealed that EWS-FLI1 depletion significantly reduces the sensitivity of EwS cells to NAMPT inhibition. Consistent with this finding, a comparison of 7 EwS cell lines of different genotypes with 5 Non-EwS cell lines and mesenchymal stem cells revealed significantly higher FK866 sensitivity of EWS-ETS positive EwS cells, with IC50 values mostly below 1nM.Taken together, our data reveal evidence of an important role of the NAMPT-mediated NAD salvage pathway in the energy homeostasis of EwS cells and suggest NAMPT inhibition as a potential new treatment approach for Ewing sarcoma.Austrian Science Fund (FWF), grant I1225-B19; European Union's FP7 project ASSET (grant agreement No. 259348); Research Manitoba and CancerCare Manitoba Foundation. Heart and Stroke Foundation of Canada (G-14-0005708). E.M.M. is the recipient of a Research Manitoba Graduate Studentship. G.M.H. is the Canada Research Chair in Molecular Cardiolipin Metabolism. J.A. is supported by Asociación Pablo Ugarte and Miguelañez S.A, ASION-La Hucha de Tomás, Fundación La Sonrisa de Alex and Instituto de Salud Carlos III (PI12/00816 and Spanish Cancer Network RTICC RD12/0036/0027).S
