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Comparison of black-box, glass-box and open-box software for aiding conceptual understanding
No full textThree mathematical software types: black-box (no steps shown), glass-box (steps shown) and open-box (interactive steps) were used by 32 students to solve conceptual and procedural tasks on the computer via remote observation. Comparison of the three software types suggests that there is no difference in the scores that students receive for conceptual understanding tasks. Students using the black-box are more likely to explore answers than students using the glass and open-box software
Evaluasi Kinerja Simpang Tak Bersinyal Jl. Rungkut Kidul – Jl. Zamhuri – Jl. Rungkut Tengah – Jl. Rungkut Industri Kidul Surabaya
No full textBerkembangnya perekonomian berbanding lurus dengan meningkatnya volume kendaraan lalu lintas di daerah Surabaya, sehingga pemerintah kota Surabaya merencanakan perbaikan manajemen lalu lintas. Seperti di daerah Rungkut Surabaya saat ini sedang diadakan proyek pelebaran jalan dengan pemasangan box culvert di Jl. Zamhuri untuk mengurangi kemacetan. Dengan selesainya proyek tersebut, kemungkinan akan menyebabkan bertambahnya volume lalu lintas pada persimpangan Jl. Rungkut Kidul – Jl. Zamhuri – Jl. Rungkut Tengah – Jl. Rungkut Industri Kidul Surabaya. Oleh karena itu, perlu dilakukan analisa dan evaluasi kinerja pada simpang serta perencanaan perbaikan simpang agar kinerja persimpangan dapat dioptimalkan sesuai rencana.
Analisa kinerja persimpangan Jl. Rungkut Kidul – Jl. Zamhuri – Jl. Rungkut Tengah – Jl. Rungkut Industri Kidul Surabaya untuk saat ini (tahun 2017) sampai dengan 5 tahun mendatang (2018 - 2022) di awali dari pengumpulan data–data jumlah penduduk, tata guna lahan, dan pertumbuhan jumlah kendaraan yang didapat dari BPS Kota Surabaya dan PU Cipta Karya Kota Surabaya sebagai data sekunder. Kemudian melakukan survey volume lalu lintas di persimpangan Jl. Rungkut Kidul – Jl. Zamhuri – Jl. Rungkut Tengah – Jl. Rungkut Industri Kidul Surabaya untuk mendapatkan data primer. Setelah semua data tersebut terkumpul, dilanjutkan dengan mengolah data secara manual berdasarkan MKJI 1997 dan menggunakan program KAJI.
Berdasarkan hasil analisa dan evaluasi kinerja simpang kondisi eksisting berupa simpang tak bersinyal pada Puncak Pagi, Puncak Siang, dan Puncak Sore didapatkan DS > 0,75 dengan Peluang antrian (QP) berkisar |29 % - 179 %| > 100 %. Dilakukan perencanaan perbaikan kinerja simpang alternative 1 dengan merencanakan pengaturan simpang bersinyal 3 fase pelebaran jalan, serta mengurangi hambatan samping menjadi kategori sedang. Pada Puncak Pagi, Puncak Siang, dan Puncak Sore tahun 2017 - 2022 diperoleh rata-rata DS < 0,75 dengan Tundaan Simpang Rata-Rata (DI) berkisar |26,58 – 39,07| det/smp dan didapatkan rata-rata LOS D. Kemudian dilakukan perencanaan perbaikan kinerja simpang alternative 2 sebagai alternative perbaikan terpilih yaitu dengan pelebaran jalan, perubahan fase, serta penyesuaian waktu sinyal. Pada Puncak Pagi, Puncak Siang, dan Puncak Sore tahun 2017 - 2022 diperoleh rata-rata DS < 0,75 dengan Tundaan Simpang Rata-Rata (DI) berkisar |22,28 – 35,79| det/smp dan didapatkan rata-rata LOS C.
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The development of economy are equal to the increase of volume traffic in Surabaya, so Surabaya’s government are planning the improvement and development of traffic management. For example, Rungkut Surabaya which currently held road widening project with box culvert’s installation at Zamhuri Street to reduce it’s traffic jam. With the completion of the project, it’s likely to cause an increase in traffic volume at the intersection of Rungkut Kidul Street – Zamhuri Street – Rungkut Tengah Street – Rungkut Industri Kidul Street Surabaya. Therefore, it’s necessary to analyze and evaluate the performance at the intersection and planning the improvement of the intersection so it can be optimized according to plan.
Performance analysis the intersection of Rungkut Kidul Street – Zamhuri Street – Rungkut Tengah Street – Rungkut Industri Kidul Street Surabaya starting in this year (2017) up to the next 5 years (2018 – 2022). This analysis start with data collection of population data, land use, and growth of vehicles obtained from BPS and PU Cipta Karya Surabaya as secondary data. Then survey data of vehicle volume in intersection area as primary data. After all data collected, then manually analysis data based on MKJI 1997 and using KAJI program.
Based on the analysis and performance evaluation of existing unsignaled intersection at peak hour in the morning, afternoon, and evening can be inferred DS > 0,75 and QP about |29 % - 179 %| > 100%. Then planning alternative repairs, the first alternative is planning signaled intersection with a 3-phase, widening the approach, and reduce the side friction to median category. At peak hour in the morning, afternoon, and evening in 2017 – 2022 obtained average of DS < 0,75 with range of Delay Intersection (DI) about |26,58 – 39,07| sec/pcu and average LOS D. The second alternative as the selected alternative is widening the approach, changes the phase, and adjust the cycle time. At peak hour in the morning, afternoon, and evening in 2017 – 2022 obtained average of DS < 0,75 with range of Delay Intersection (DI) about |22,28 – 35,79| sec/pcu and average LOS C
Perencanaan Simpang Bersinyal Di Jl. Raya Berbek – Jl. Wadung Asri – Jl. Gedong Masjid, Kab. Sidoarjo
No full textKabupaten Sidoarjo memiliki jumlah penduduk yang cukup
padat dengan permasalahan transportasi yang kompleks. Salah satunya adalah kemacetan pada ruas jalan dan ketidak teraturan pada simpang tak bersinyal yang terletak di Jl. Raya Berbek – Jl. Wadung Asri – Jl. Gedong Masjid, Kabupaten Sidoarjo. Simpang tersebut dekat dengan area komersil pada sekitar bahu jalan, pasar yang berlokasi di dekat U-turn, dan pengaturan simpang tersebut menggunakan tenaga manusia.
Permasalahan, bagaimana kinerja lalu lintas pada lokasi studi tahun 2018 dan 2023.
Metode penelitian yang digunakan dalam pengambilan data
adalah pengamatan dan pencatatan hasil pengamatan. Analisis dalam penelitian sebegai bentuk pengolahan serta penyelesaian data mengacu pada Manual Kapasitas Jalan Indonesia (MKJI) tahun 1997 untuk mengetahui tingkat pelayanan ruas jalan dan simpang tak bersinyal, serta mengevaluasinya menjadi simpang
bersinyal.
Hasil analisa kinerja ruas jalan menunjukkan DS = 0,57
dengan LOS C dan simpang tak bersinyal dengan LOS F dan
tundaan simpang 30,64 det/smp. Perbaikan dengan bantuan
program KAJI didapat LOS C dan tundaan simpang 16,56 det/smp. Hasil perbaikan tersebut didapat dari pengaturan lampu lalu-lintas dengan waktu hijau fase 1 dan fase 2 masing-masing 33 detik dan 20 detik, serta dengan pengaturan lebar lajur lalu-lintas untuk masing-masing pendekat. Selain itu diperlukan juga pelebaran dengan box culvert pada Jl. Gedong Masjid yang mulanya 4,21 meter menjadi 7,20 meter dapat membantu meningkatkan kinerja
simpang tersebut.
Kata Kunci: simpang tak bersinyal, simpang bersinyal, ruas jalan, Pelebaran dengan box culvert
=========================================================Sidoarjo has population which is quite dense with complex
transportation issues. One of them is congestion on the road and irregularity on the unsignals intersection that located on Raya Berbek ST. – Wadung Asri ST. – Gedong Masjid ST., Sidoarjo. That intersection close to the comercial area, market that located near the U-turn, and that arrange by human power.
Concerns, how is the performance of the traffic on it from 2018 to 2023.
Research methods that used in data taking is the observation and recording of the observation result. Analysis in the research as a form of processing and the completion of the data refers to the Indonesia's Road Capacity Manual (MKJI) in 1997 to find out the level of service roads and unsignals intersection, as well as
evaluate became signals intersection.
The analysis results of performance of road shows DS =
0.57 with LOS C and unsignals intersection with LOS F and
intersections delay 30.64 sec/pcu. Improvements using program KAJI obtained LOS C intersections delay 16.56 sec/pcu. The results of these improvements gained from traffic lights setting with the green time phase 1 and phase 2 each 33 seconds and 20 seconds, as well as with arrange the width of the traffic lanes for each approach. In addition it is also required widening with box
culvert on Gedong Masjid ST. from 4.21 meters to 7.20 meters, it will improve the performance of the intersection.
Keyword: unsignals intersection, signal intersection, roads, widening with box culver
Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans
No full textBackground: The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.Results: Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.Conclusion: In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein
STIL binding to Polo-box 3 of PLK4 regulates centriole duplication
No full textPolo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication
Analisa Kinerja Marka Yellow Box Junction Pada Persimpangan Jl. Imam Bonjol – Jl. Jenderal Sudirman – Jl. Mongonsidi (Medan) (Studi Kasus)
No full textYellow box junction adalah kotak kuning bujur sangkar yang memiliki garis
diagonal yang saling berhubungan di lengan jalan, yang bertujuan untuk
memecahkan kondisi lalu lintas yang sering macet di sejumlah titik khususnya di
perkotaan dan persimpangan. Yellow box sudah banyak di pakai di Indonesia,
pemakaian yellow box di kota Medan dapat dilihat pada persimpangan jalan Imam
Bonjol, Jalan Jenderal Sudirman dan Jalan Mongonsidi. Dan untuk mengetahui
kinerja lalu lintas pada persimpangan bersinyal Jalan Imam Bonjol, Jalan Jenderal
Sudirman dan Jalan Mongonsidi sesuai dengan Manual Kapasitas Jalan Indonesia
(MKJI) Tahun 1997. Perilaku lalu lintas persimpangan kondisi eksisting Jalan
Imam Bonjol (pendekat Utara) adalah, volume (Q) 741 smp/jam, kapasitas 824
smp/jam, DS (Derajat Kejenuhan) 0.899, panjang antrian 200.0 m, jumlah
kendaraan terhenti 702 smp/jam, dan tundaan rata-rata 98.5 det/smp, simpang
Jalan Jenderal Sudirman (pendekat utara) adalah, volume (Q) 392 smp/jam,
kapasitas 393 smp/jam, DS (Derajat Kejenuhan) 0.997, panjang antrian 333.3 m,
jumlah kendaraan terhenti 487 smp/jam, dan tundaan rata-rata 178,7 det/smp, dan
simpang Jalan Mongonsidi (pendekat Selatan) adalah, volume (Q) 489 smp/jam,
kapasitas 430 smp/jam, DS (Derajat Kejenuhan) 1,137, panjang antrian 419.0 m,
jumlah kendaraan terhenti 1504 smp/jam, dan tundaan rata-rata 321.8 det/smp.
Pelanggaran yellow box junction simpang Imam Bonjol sebesar 5,64%, simpang
Jenderal Sudirman sebesar 8,82% dan simpang mongonsidi sebesar 7,73%.
Lengan jalan memiliki tingkat pelayanan C. Berdasarkan hasil survei yang telah
diamati secara langsung dapat dinyatakan bahwa angka pelanggaran dari para
pengendara kendaraan bermotor terhadap penempatan marka YBJ adalah cukup
rendah. Dapat disimpulkan bahwa pemakaian yellow box di kota Medan sudah
tepat. Akan tetapi pelanggaran yang terjadi di yellow box masih banyak terjadi
terutama oleh para pengguna sepeda motor
Perencanaan Simpang Bersinyal Di Jl. Raya Berbek – Jl. Wadung Asri – Jl. Gedong Masjid, Kab. Sidoarjo
No full textKabupaten Sidoarjo memiliki jumlah penduduk yang cukup padat dengan permasalahan transportasi yang kompleks. Salah satunya adalah kemacetan pada ruas jalan dan ketidak teraturan pada simpang tak bersinyal yang terletak di Jl. Raya Berbek – Jl. Wadung Asri – Jl. Gedong Masjid, Kabupaten Sidoarjo. Simpang tersebut dekat dengan area komersil pada sekitar bahu jalan, pasar yang berlokasi di dekat U-turn, dan pengaturan simpang tersebut menggunakan tenaga manusia. Permasalahan, bagaimana kinerja lalu lintas pada lokasi studi tahun 2018 dan 2023.
Metode penelitian yang digunakan dalam pengambilan data adalah pengamatan dan pencatatan hasil pengamatan. Analisis dalam penelitian sebegai bentuk pengolahan serta penyelesaian data mengacu pada Manual Kapasitas Jalan Indonesia (MKJI) tahun 1997 untuk mengetahui tingkat pelayanan ruas jalan dan simpang tak bersinyal, serta mengevaluasinya menjadi simpang bersinyal.
Hasil analisa kinerja ruas jalan eksisting menunjukkan DS = 0,57 dengan LOS C dan simpang tak bersinyal dengan LOS F dan tundaan simpang 30,64 det/smp. Perbaikan menjadi simpang bersinyal dengan bantuan program KAJI didapat LOS E dengan mean number of stops 0,75 , panjang antrian sepanjang 157 meter untuk pendekat selatan, dan tundaan simpang 43,55 det/smp. Hasil perbaikan tersebut didapat dari pengaturan lampu lalu-lintas dengan cycle time 120 detik serta dengan pengaturan lebar lajur lalu-lintas untuk masing-masing pendekat. Selain itu diperlukan juga pelebaran dengan box culvert pada Jl. Gedong Masjid yang mulanya 4,21 meter menjadi 7,20 meter sepanjang 43 meter dapat membantu meningkatkan kinerja simpang tersebut.
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Sidoarjo has population which is quite dense with complex transportation issues. One of them is congestion on the road and irregularity on the unsignals intersection that located on Raya Berbek ST. – Wadung Asri ST. – Gedong Masjid ST., Sidoarjo. That intersection close to the comercial area, market that located near the U-turn, and that arrange by human power.
Concerns, how is the performance of the traffic on it from 2018 to 2023.
Research methods that used in data taking is the observation and recording of the observation result. Analysis in the research as a form of processing and the completion of the data refers to the Indonesia's Road Capacity Manual (MKJI) in 1997 to find out the level of service roads and unsignals intersection, as well as evaluate became signals intersection..
The analysis results of performance of the existing road shows DS = 0.57 with LOS C and unsignals intersection with LOS F and intersections delay 30.64 sec/pcu. Improvements using program KAJI obtained LOS E with a mean number of stops of 0.75, a queue length of 157 meters for the southern approach, and a intersection delay of 43.55 seconds / pcs The results of these improvements gained from The results of these repairs are obtained from setting up traffic lights with a 120 second cycle time, as well as with arrange the width of the traffic lanes for each approach. In addition it is also required widening with box culvert on Gedong Masjid ST. from 4.21 meters to 7.20 meters along 43 meters , it will improve the performance of the intersection
DDX10 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 10)
No full textReview on DDX10 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 10), with data on DNA, on the protein encoded, and where the gene is implicated
STUDI ALTERNATIF PERENCANAAN STRUKTUR ATAS FLYOVER MANAHAN DENGAN KONSTRUKSI BOX GIRDER PRATEGANG METODE SPAN-BY-SPAN
No full textPerencanaan jembatan layang Manahan menggunakan box girder prategang dengan system external tendon untuk mengefisiensikan kombinasi antara volume beton yang relative kecil dengan gaya prategang tendon sehingga didapat lendutan yang relative kecil pada bentang. Penggunaan box girder prategang lebih menguntungkan dibandingkan balok I – girder pada perlintasan sebidang jalur rel kereta api Solo – Yogyakarta setelah simpang Jl. Adi Sucipto - Jl. MT Hariyono ke arah Jl. Dr. Moewardi, Kota Surakarta dikarenakan metode pelaksanaan balok I – girder lebih menyebabkan macet.
Analisa statika menggunakan statis tertentu dan metode pelaksanaan secara segmental (metode span-by-span) untuk mendapatkan section properties dari penampang box girder. Peraturan yang penulis gunakan dalam perhitungan adalah SNI 1725-2016, SNI 2847-2013. Materi pembahasan meliputi perencanaan tata letak tendon, kehilangan gaya prategang, kontrol tegangan dan lendutan, penulangan box girder-geser, dan end block. Kabel prategang yang digunakan adalah tendon VSL type GC Grade 270, low relaxation strand dengan tipe 6-37 unit.
Hasil yang diperoleh dari perencanaan box girder prategang ialah, digunakan 20 segmental yang terbagi menjadi 3 jenis segmen yaitu segmen tumpuan, segmen deviator, dan segmen strandar. Menggunakan 12 buah tendon VSL tipe GC dengan 35 strand tiap tendon. Adapun lendutan yang terjadi sebesar 5,194 cm (kebawah)dan tidak terjadi tegangan tarik
Functional analysis and binding affinity of tomato ethylene response factors provide insight on the molecular bases of plant differential responses to ethylene
No full textBackground : The phytohormone ethylene is involved in a wide range of developmental processes and in mediating plant responses to biotic and abiotic stresses. Ethylene signalling acts via a linear transduction pathway leading to the activation of Ethylene Response Factor genes (ERF)which represent one of the largest gene families of plant transcription factors. How an apparently simple signalling pathway can account for the complex and widely diverse plant responses to ethylene remains yet an unanswered question. Building on the recent release of the complete tomato genome sequence, the present study aims at gaining better insight on distinctive features among ERF proteins. Results : A set of 28 cDNA clones encoding ERFs in the tomato (Solanum lycopersicon) were isolated and shown to fall into nine distinct subclasses characterised by specific conserved motifs most of which with unknown function. In addition of being able to regulate the transcriptional activity of GCC-box containing promoters, tomato ERFs are also shown to be active on promoters lacking this canonical ethylene-responsive-element. Moreover, the data reveal that ERF affinity to the GCC-box depends on the nucleotide environment surrounding this cis-acting element. Site-directed mutagenesis revealed that the nature of the flanking nucleotides can either enhance or reduce the binding affinity, thus conferring the binding specificity of various ERFs to target promoters. Based on their expression pattern, ERF genes can be clustered in two main clades given their preferential expression in reproductive or vegetative tissues. The regulation of several tomato ERF genes by both ethylene and auxin, suggests their potential contribution to the convergence mechanism between the signalling pathways of the two hormones. Conclusions : The data reveal that regions flanking the core GCC-box sequence are part of the discrimination mechanism by which ERFs selectively bind to their target promoters. ERF tissue-specific expression combined to their responsiveness to both ethylene and auxin bring some insight on the complexity and fine regulation mechanisms involving these transcriptional mediators. All together the data support the hypothesis that ERFs are the main component enabling ethylene to regulate a wide range of physiological processes in a highly specific and coordinated manner
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