8 research outputs found
Preparation of 3[(-carboxyphenyl) methylene] -hydrazinecarboxamide for immunoassay of nitrofuran antibiotics
No full textCurrently, analysis of Semicarbazide (SEM) is being carried out using LC-MS/MS instrumentation. It is a need to developed rapid immuno based test kit. We described the synthesis of modified SEM hapten has been carried out through chemical reaction. Polyclonal antibody (pAb) was produced to detect Semicarbazide (SEM), metabolite as a marker residue of nitofurazone in animal food production. A carboxyphenyl derivative of SEM was synthesized following derivatisation of 4-carboxylbenzaldehyde (4-CBA). The modified SEM hapten was characterized using thin layer chromatography (TLC) with Rf value of 0.53 in crystalline form. The fourier transform infra-red (FTIR) spectrum exhibits the peaks at Vmax 3584cm-1 for C=O (carboxylic group). The melting point for the synthesized hapten is 220-223oC. After purification of hapten, the newly developed hapten was then conjugated to keyhole limpet hemocyanin (KLH) as immunogen using 1-ethtyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) method for conjugation purpose. This immunogen (SEM-KLH) were used to immunize two New Zealand white rabbit to produce polyclonal antibody against newly developed hapten. Using direct enzyme linked immunoassay (ELISA) method, titer determination of the developed antibody of 0.01mg/ml was obtained. For conjugation efficiency electrophoresis gel SDS-PAGE were carried out using 10-7% of resolving gel. This is to identify protein band according to commercial protein marker. No band occur for both of this resolving gel concentration but band for KLH occur at 7% resolving gel but no band for conjugated hapten-protein. Another confirmation of the developed antibody efficiency test is done using surface plasmon resonance which a well is known as biosensor instrument. According to the result, limit of detection for this antibody is up to 0.1 x 10-7 mg/ml compared to ELISA metho
Aktualisasi Revolusi Mental dalam Pengelolaan Dana Desa (Analisis Tafsir Ekonomi Q.S.Yusuf [12]: 55)
No full textDana Desa are one of the most effective policies in village development with the completion of various kinds of infrastructure in the village. On the other hand, this policy caused side effects in the form of criminal acts of corruption against these funds. Through the interpretation of Q.S. Yusuf verse 55, the author offers the actualization of the spirit of mental revolution in the management of village funds through measures to strengthen integrity, increase professionalism, innovation, responsibility, and broaden horizons. Through these steps, the village fund policy can be implemented completely and right on target
Validation of developed antibody using surface plasm on resonance for determination of nitrofuran residues in poultry
No full textComparative Study of Pesticides Analysis Using Enzyme Inhibition Sensor and Gas Chromatography Methods
No full textAbstractPesticides are widely used in paddy field to control pests, diseases, weeds and other rice pathogens in minimizing a serious loss in rice production. The presence of pesticide residues and metabolites in rice, water, soil, currently represents a major environmental pollutant issues. It sometime will cause insect pest outbreaks in paddy field. An electrochemical enzyme inhibition sensor using screen-printed carbon working electrode with onboard carbon counter and silver–silver chloride pseudo-reference electrode for insecticides detection is described in this paper (Figure 1). The detection is based on the inhibition of insecticides used in paddy field towards acetyl-cholinesterase enzyme (AChE) with the presence of Acetylthiocholine Iodide (ATCh) substrate on the sensor surface. The mixtures of AChE enzyme (0.02 UmL-1), electron mediator, TCNQ (1mM) and polypyrrole matrix (75 mM) were electro- polymerized on the sensor surface with a constant potential of 1.0V for 20 minutes. The sensor was soaked for 5 minutes with insecticides standard or sample containing insecticides before the electrochemical measurement was taken by adding Acetylthiocholine Iodide substrate in KCl (0.08M, pH 7.5) which acts as the enzyme mediator /substrate system. The current measurement was conducted using chronoamperometry at 100mV vs. on board screen-printed Ag-AgCl pseudo- reference electrode. Comparative analysis of spiked water samples with 0.1ppm pesticides and real samples (paddy) also were conducted using enzyme inhibition sensor and gas chromatography methods. From the data analysis, it showed very comparable results with R2 = 0.96 in the correlation plot for paddy samples samples. This makes the developed sensor a potential tool for the rapid, simple and sensitive detection of insecticides residues in agriculture industry
IDENTIFIKASI SENYAWA FITOKIMIA DAN EVALUASI PENGARUH PEMANASAN TERHADAP DAYA ANTIOKSIDAN BUMBU-BUMBU RENDANG
No full textIDENTIFIKASI SENYAWA FITOKIMIA
DAN EVALUASI PENGARUH PEMANASAN TERHADAP DAYA ANTIOKSIDAN BUMBU-BUMBU RENDANG
Fauzan Azima, Tutty Anggraini, Nining Sriwahyuni
Fakultas Teknologi Pertanian, Jurusan Pascasarjana TIP
Corrresponding author; email: [email protected]
ABSTRAK
Penelitian ini bertujuan untuk mengetahui senyawa fitokimia bumbu-bumbu yang digunakan dalam pembuatan rendang, mengetahui kadar bahan aktif penyusun bumbu-bumbu rendang, mengetahui daya antioksidan masing-masing bumbu rendang, dan produk rendang (gulai, kalio, rendang basah, dan rendang kering). Penelitian menggunakan Rancangan Acak Lengkap (RAL) terdiri dari 4 perlakukan dan 3 kali ulangan. Data pengamatan dianalisis dengan uji F, dan uji lanjut Duncan’s New Multiple Range Test (DNMRT) pada taraf nyata 5%. Perlakuan pada penelitian ini adalah lama pemanasan masing-masing A = pemanasan selama 30 menit sampai terbentuk gulai, B = pemanasan lanjutan selama 30 menit sampai terbentuk kalio C = pemanasan lanjutan selama 30 menit sampai terbentuk rendang basah, D = pemanasan lanjutan selama 30 menit sampai terbentuk rendang kering. Hasil penelitian menunjukkan bumbu rendang mengandung senyawa fitokimia ( fenolilk, alkaloid, flavonoid, terpenoid, dan saponin). Jumlah komponen utama yang didapatkan pada bawang merah, cabe merah, cengkeh, daun jeruk, daun kunyit, daun salam, jahe, jintan, kayu manis, kemiri, ketumbar, lengkuas, pala, dan sereh berturut-turut yaitu inulin 2695.5 ppm, kapsaisin 49.5 ppm, eugenol 57.09 %, trans-cinnamyl acetate 13.32%, kurkumin 2.057 ppm, eugenol 3.43%, zingiberine 2.84%, safranal 2152.2 ppm, sinamaldehid 4.52%, linolenic acid 49.91 %, linalool 7.03 %, sineol 1.07 %, miristin 0.02 ppm, ascorbic acid 31.82 %. Nilai aktifitas antioksidan ekstrak rempah-rempah berkisar antara 15.29 – 63.37%. Bumbu rendang dengan penambahan santan berkisar 1.31 – 26.03%, cengkeh memiliki aktifitas antioksidan tertinggi pada ekstrak rempah dengan nilai aktivitas antioksidan 63.37% dan diikuti cabe 58.16%, bawang putih, 50.12%, kayu manis 43.73%, sereh 36.33%, jintan 35.69%, daun jeruk purut 35.60%, lengkuas 22.77%, ketumbar 22.69%, pala 21.64%, daun salam 20.73%, jahe 16.92%, kemiri 16.74%, bawang merah 16.09%, daun kunyit 15.29%. Perkiraan masa simpan rendang berdasarkan hasil pengamatan dengan rancimat 743; kalio 0.24 bulan, rendang basah 2.7 bulan, dan rendang kering 6.8 bulan.
Kata kunci : antioksidan, rempah-rempah, pemanasan, senyawa aktif fitokimi
Successful Treatment of Refractory Vitiligo with a Combination of Khellin and 308-nm Excimer Lamp: An Open-Label, 1-Year Prospective Study
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Operation of a free-electron laser from the extreme ultraviolet to the water window
No full textWe report results on the performance of a free-electron laser operating at a wavelength of 13.7 nm where unprecedented peak and average powers for a coherent extreme-ultraviolet radiation source have been measured. In the saturation regime, the peak energy approached 170 J for individual pulses, and the average energy per pulse reached 70 J. The pulse duration was in the region of 10 fs, and peak powers of 10 GW were achieved. At a pulse repetition frequency of 700 pulses per second, the average extreme-ultraviolet power reached 20 mW. The output beam also contained a significant contribution from odd harmonics of approximately 0.6% and 0.03% for the 3rd (4.6 nm) and the 5th (2.75 nm) harmonics, respectively. At 2.75 nm the 5th harmonic of the radiation reaches deep into the water window, a wavelength range that is crucially important for the investigation of biological samples
A decade of amphibian studies (Animalia, Amphibia) at Sekayu lowland forest, Hulu Terengganu, Peninsular Malaysia
No full textAmphibians of Sekayu lowland forest have been studied more than a decade, with discoveries of new records of species showing no sign of abating between the years 2003 to 2020, indicating the remarkably rich diversity of anurans in this forest. Despite ceaseless anthropogenic activities in this area, this study successfully recorded 52 species of amphibians from 32 genera in the lowland forest of Sekayu. The species composition consisted of a single species from the family Ichthyophiidae and 51 species of anurans of 31 genera and six families. The number of species recorded has steadily increased especially during more recent surveys from 2015 to 2020. This study augments the total number of amphibian species recorded from Hulu Terengganu by ten additional species, increasing the total to 70 species for the district
