1,928,404 research outputs found
Phospholipases A1
No full textResearch in the author's laboratory is supported in part by a Wellcome Trust Senior Research Fellowship (067441), and Wellcome Trust project grants (086658 and 093228) and a Wellcome Trust Prize Studentship (G.S.R).Phospholipase A1 (PLA1) is an enzyme that hydrolyzes phospholipids and produces 2-acyl-lysophospholipids and fatty acids. This lipolytic activity is conserved in a wide range of organisms but is carried out by a diverse set of PLA1 enzymes. Where their function is known, PLA1s have been shown to act as digestive enzymes, possess central roles in membrane maintenance and remodeling, or regulate important cellular mechanisms by the production of various lysophospholipid mediators, such as lysophosphatidylserine and lysophosphatidic acid, which in turn have multiple biological functions.Peer reviewe
A1 Keto BHB Weight Loss Reviews
No full textA1 Keto BHB In the event that you need to shed pounds consistently, the best way to deal with help as such is to join out weight decrease supplement in A1 Keto BHB your ordinary eating routine, so in the event that you're ready to exploit it, endeavor Keto Benefit. This is a trademark weight decrease game plan that helps you with remaining on track with your food and exercise so you may show up at your objections, work on your A1 Keto BHB energy, and achieve your weight decline goals
Interação entre os sistemas adenosinérgico e endocanabinóide, através dos receptores A1 e CB1, no controle da memória espacial
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em FarmacologiaOs sistemas endocanabinóide e adenosinérgico, através dos receptores A1 e CB1, compartilham uma série de características. Ou seja, ambos os receptores ativam proteínas Gi/o para mediar suas principais ações, inibem a liberação de diferentes neurotransmissores e são expressos em grandes concentrações no córtex, hipocampo e cerebelo. Além disso, agonistas dos dois receptores prejudicam processos relacionados à memória espacial enquanto que antagonistas induzem melhora cognitiva. Apesar das semelhanças entre os dois sistemas e da importância da memória espacial na fisiopatologia de diferentes doenças, poucos estudos avaliaram a possível interação entre ambos os sistemas no controle da memória espacial. Desta forma o objetivo deste trabalho foi avaliar a interação entre os sistemas adenosinérgico e endocanabinóide, através dos receptores A1 e CB1, na modulação da memória espacial. Para isso validou-se o modelo da localização de objetos em camundongos, o qual pareceu sensível a drogas amnésicas ou pró-mnemônicas. Além disso, demonstrou-se que a região CA1 do hipocampo participa da aquisição da memória de localização de objetos em camundongos. Posteriormente, utilizou-se o modelo recém validado e o labirinto aquático para avaliar o efeito pró-mnemônico da co-administração de antagonistas dos receptores A1 e CB1. Esta interação melhora o aprendizado de camundongos em doses até 20 vezes menores do que aquelas utilizadas para produzir o mesmo efeito quando administradas isoladamente. Na sequência demonstrou-se que as ações da co-administração são mediadas, pelo menos em parte, pela liberação de glutamato e a interação deste com os receptores NMDA no hipocampo e no córtex pré-frontal. No último grupo de experimentos, demonstrou-se que os receptores A1 e CB1 do hipocampo interagem para modular a amnésia induzida por agonistas de ambos. Ou seja, o efeito amnésico do agonista A1 é bloqueado pelo antagonista CB1, enquanto que o prejuízo de aprendizado induzido pelo agonista CB1 é atenuado pelo agonista A1. Sugerindo que tanto o tônus adenosinérgico quanto o endocanabinóide interagem para a modulação da amnésia induzida por agonistas dos receptores CB1 e A1, respectivamente. Por fim, conclui-se que os sistemas adenosinérgico e endocanabinóide, através de seus receptores A1 e CB1, interagem para o controle de processos associados à memória espacial de camundongos.The adenosinergic and endocannabinoid systems share different characteristics through the A1 and CB1 receptors. For example, both are densely expressed in areas such as the cerebellum, prefrontal cortex, and hippocampus. The binding of agonists to them activates Gi/o proteins and similar mechanisms of signal transduction. Functionally, A1 and CB1 receptor activation inhibits the release of different neurotransmitters. In animal models of spatial learning, A1 and CB1 receptor agonists and antagonists have been reported to impair and to facilitate learning and memory, respectively. Despite these evidences, there are no studies evaluating the interaction between both systems in spatial memory processes. Initially, we have done a pharmacological validation of the object-location task in mice. This task is sensitive to amnesic and pro-mnemonic drugs, and the expression of memory seems to be dependent on the CA1 hippocampus. In a second set of results, we have shown that coadministration of subeffective doses of A1 and CB1 antagonists improves acquisition of spatial learning evaluated either in the water maze or in the object-location task. This effect was dependent on glutamate release into the hippocampus and prefrontal cortex. The blockade of NMDA receptors in the CA1 hippocampus and prefrontal cortex counteracts the effects of the coadministration evaluated in the object-location task. This suggests that the simultaneous blockade of the adenosinergic and endocannabinoid tonuses might enhance memory, and that the mechanism of this effect is dependent on glutamate release into the hippocampus and prefrontal cortex. The last group of experiments evaluated the interaction between A1 and CB1 receptors in the amnesia induced by the agonists of both. The result suggests that the amnesia induced by an A1 agonist is blocked by a CB1 receptor antagonist. On the other hand, the learning deficits induced by the CB1 receptor agonist are attenuated by the A1 receptor activation. This suggests that the adenosinergic and endocannabinoid systems might modulate amnesia induced either by A1 or CB1 agonists. Finally, these results also suggest that the adenosinergic and endocannabinoid systems interact to modulate behavioral processes associated with spatial memory
A1-indeuced mutation fingerprints of ERVs in Galliformes.
No full textA. Distribution of A1 genes in Galliformes. The normal expression and pseudogeneization of the A1 gene in the species are represented by solid circles and open circles, respectively. B. The density of genetic distance D between 5'-LTR and 3'-LTR of ERVs in six Galliformes. The solid blue line represents the average genetic distance between homologous introns between Gallus gallus and Penelope pileata, and the dashed lines at both ends represent the 95% confidence interval. C. A1-induced mutational signatures of ERVs before A1 pseudogenization in all 6 species of Galliformes. </p
Long-term results of suture annuloplasty for degenerative mitral valve disease: a propensity-matched analysis
No full textAIMS:
Ring annuloplasty is the gold standard of surgical repair in degenerative mitral valve disease. However, prosthetic annuloplasty has some drawbacks and potential hazards. Suture annuloplasty theoretically is able to preserve annular leaflet dynamics and left ventricular performance, but experience is limited. The aim of the study was to review the early and long-term outcome of the posterior double-suture annuloplasty (DSA) technique for degenerative mitral valve repair.
METHODS:
From January 2002 to December 2008, 400 patients underwent primary mitral valve repair for degenerative disease either with posterior DSA [n = 147 (37%)] or with flexible posterior annuloplasty band [n = 253 (63%)]. Differences in patient characteristics were addressed by propensity-score matching (132 pairs). A composite end-point of mitral valve failure (MVF) was calculated as the incidence of mitral valve regurgitation greater than 2+ or need for mitral valve replacement at follow-up.
RESULTS:
After propensity-score matching, the distribution of preoperative variables among matched pairs was, on average, equal. Isolated annuloplasty and leaflet repair techniques were similarly performed in both groups (P = 0.20). In-hospital mortality was comparable between the two study groups (P = 0.48). Predischarge echocardiography showed excellent results regarding valve hemodynamics (P = 0.71). At a mean follow-up of 11 ± 3 years, all-cause mortality (P = 0.12), need for mitral valve replacement (P = 0.49), and cardiac re-hospitalization rate (P = 0.57) resulted comparable between the two groups. Ten-year survival (75 vs. 71%, P = 0.51) and freedom from MVF (92 vs. 84%, P = 0.39) were similar between posterior annuloplasty band and DSA groups.
CONCLUSION:
Suture annuloplasty demonstrated comparable results with posterior flexible band repair and could be a viable option for mitral valve surgery in selected patients, such as in the minimally invasive approach, in endocarditis, and in developing countries.AIMS:
Ring annuloplasty is the gold standard of surgical repair in degenerative mitral valve disease. However, prosthetic annuloplasty has some drawbacks and potential hazards. Suture annuloplasty theoretically is able to preserve annular leaflet dynamics and left ventricular performance, but experience is limited. The aim of the study was to review the early and long-term outcome of the posterior double-suture annuloplasty (DSA) technique for degenerative mitral valve repair.
METHODS:
From January 2002 to December 2008, 400 patients underwent primary mitral valve repair for degenerative disease either with posterior DSA [n = 147 (37%)] or with flexible posterior annuloplasty band [n = 253 (63%)]. Differences in patient characteristics were addressed by propensity-score matching (132 pairs). A composite end-point of mitral valve failure (MVF) was calculated as the incidence of mitral valve regurgitation greater than 2+ or need for mitral valve replacement at follow-up.
RESULTS:
After propensity-score matching, the distribution of preoperative variables among matched pairs was, on average, equal. Isolated annuloplasty and leaflet repair techniques were similarly performed in both groups (P = 0.20). In-hospital mortality was comparable between the two study groups (P = 0.48). Predischarge echocardiography showed excellent results regarding valve hemodynamics (P = 0.71). At a mean follow-up of 11 ± 3 years, all-cause mortality (P = 0.12), need for mitral valve replacement (P = 0.49), and cardiac re-hospitalization rate (P = 0.57) resulted comparable between the two groups. Ten-year survival (75 vs. 71%, P = 0.51) and freedom from MVF (92 vs. 84%, P = 0.39) were similar between posterior annuloplasty band and DSA groups.
CONCLUSION:
Suture annuloplasty demonstrated comparable results with posterior flexible band repair and could be a viable option for mitral valve surgery in selected patients, such as in the minimally invasive approach, in endocarditis, and in developing countrie
Endogenous annexin A1 counter-regulates bleomycin-induced lung fibrosis
No full textAbstract Background The balancing functions of pro/anti-inflammatory mediators of the complex innate responses have been investigated in a variety of experimental inflammatory settings. Annexin-A1 (AnxA1) is one mediator of endogenous anti-inflammation, affording regulation of leukocyte trafficking and activation in many contexts, yet its role in lung pathologies has been scarcely investigated, despite being highly expressed in lung cells. Here we have applied the bleomycin lung fibrosis model to AnxA1 null mice over a 21-day time-course, to monitor potential impact of this mediator on the control of the inflammatory and fibrotic phases. Results Analyses in wild-type mice revealed strict spatial and temporal regulation of the Anxa1 gene, e.g. up-regulation in epithelial cells and infiltrated granulocytes at day 7, followed by augmented protein levels in alveolar macrophages by day 21. Absence of AnxA1 caused increases in: i) the degree of inflammation at day 7; and ii) indexes of fibrosis (assessed by deposition of hydroxyproline in the lung) at day 7 and 21. These alterations in AnxA1 null mice were paralleled by augmented TGF-β1, IFN-γ and TNF-α generation compared to wild-type mice. Finally, treatment of wild type animals with an AnxA1 peptido-mimetic, given prophylactically (from day 0 to 21) or therapeutically (from day 14 onward), ameliorated both signs of inflammation and fibrosis. Conclusion Collectively these data reveal a pathophysiological relevance for endogenous AnxA1 in lung inflammation and, more importantly, fibrosis, and may open new insights for the pharmacological treatment of lung fibrosis.</p
Potilasopas : akuuttipsykiatrian osasto A1
No full textPsykiatriseen osastohoitoon liittyy edelleen paljon erilaisia stigmoja ja pelkoja, mitkä saattavat heikentää tai jopa estää myönteisiä hoitotuloksia. Pelkojen vähentäminen sekä turvallisuudentunteen ja tiedon lisääminen heti osastohoidon alussa parantaa potilaiden luottamusta ja sitoutumista hoitoonsa.
Opinnäytetyön tehtävänä oli luoda potilasopas jaettavaksi Tyksin akuuttipsykiatrian osaston A1 uusille potilaille. Opinnäytetyön tavoitteena on selkiyttää osaston toimintatapoja potilaille ja helpottaa potilaiden osastolla toimimista. Potilasopas luo myös yhteneväiset toimintatavat ja säännöt, mitkä auttavat henkilökuntaa, potilaita sekä heidän läheisiään.
Potilasopas sisältää perustiedot osaston toiminnasta, henkilökunnasta sekä potilaan oikeuksista ja velvollisuuksista. Opas on potilaslähtöinen: selkeä ja informatiivinen sisältäen olennaiset tiedot osaston toiminnasta ja se on sävyltään helposti lähestyttävä ja ystävällinen. Opas toimii hyvänä tukena suullisen ohjeistuksen rinnalla. Potilasopas jaetaan fyysisenä oppaana, johon potilaan on helppo palata myöhemmin uudelleen. Oppaasta on myös helposti päivitettävä digitaalinen versio.
Potilasoppaan avulla lisätään tietoa ja hoidon läpinäkyvyyttä, mikä vähentää potilaiden pelkoa ja ennakkoluuloja. Opas myös helpottaa läheisten integrointia potilaan hoitoon. Jatkossa potilasopasta voidaan hyödyntää opiskelijoiden ja henkilökunnan perehdytyksessä
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The schematic diagram of Anx-A1-enhanced HSV-1 attachment to cells.
No full textAnx-A1 binds HSV-1 gE, and Anx-A1 on virions enhances HSV-1 attachment in a versatile manner. Virion Anx-A1 either interacts with Anx-A1 on the cell surface or with FPR2. Depletion of extracellular Anx-A1 or blocking FPR2 via WRW4 inhibits HSV-1 attachment to cells. Created with BioRender.com.</p
- …
