41 research outputs found
Perinatale Betreuung an der Grenze der Lebensfähigkeit zwischen 22 und 26 vollendeten Neugeborenen
No full textDie Betreuung und Reanimation des Neugeborenen
Get PDFEntstehung und Anwendung dieser Empfehlungen Eine Arbeitsgruppe der Schweizerischen Gesellschaft für Neonatologie (SGN) hat im Jahr 2000 Empfehlungen zur Betreuung und Reanimation von Neugeborenen für die Schweiz ausgearbeitet. Nach einer ersten Überarbeitung 2007 werden diese nun aufgrund neuerer Daten und Evidenzen1) sowie Revisionen internationaler Empfehlungen2)– 7) erneut revidiert. Diese Leitlinien sollen als Empfehlungen verstanden werden, die im individuellen Fall angepasst werden können und sollen
Protein-RNA interactions: a structural analysis
No full textA detailed computational analysis of 32 protein-RNA complexes is presented. A number of physical and chemical properties of the intermolecular interfaces are calculated and compared with those observed in protein-double-stranded DNA and protein-single-stranded DNA complexes. The interface properties of the protein-RNA complexes reveal the diverse nature of the binding sites. van der Waals contacts played a more prevalent role than hydrogen bond contacts, and preferential binding to guanine and uracil was observed. The positively charged residue, arginine, and the single aromatic residues, phenylalanine and tyrosine, all played key roles in the RNA binding sites. A comparison between protein-RNA and protein-DNA complexes showed that whilst base and backbone contacts (both hydrogen bonding and van der Waals) were observed with equal frequency in the protein-RNA complexes, backbone contacts were more dominant in the protein-DNA complexes. Although similar modes of secondary structure interactions have been observed in RNA and DNA binding proteins, the current analysis emphasises the differences that exist between the two types of nucleic acid binding protein at the atomic contact level
Prediction of protein-protein interaction types using association rule based classification
No full textThis article has been made available through the Brunel Open Access Publishing Fund - Copyright @ 2009 Park et alBackground: Protein-protein interactions (PPI) can be classified according to their characteristics into, for example obligate or transient interactions. The identification and characterization of these PPI types may help in the functional annotation of new protein complexes and in the prediction of protein interaction partners by knowledge driven approaches. Results: This work addresses pattern discovery of the interaction sites for four different interaction types to characterize and uses them for the prediction of PPI types employing Association Rule Based Classification (ARBC) which includes association rule generation and posterior classification. We incorporated domain information from protein complexes in SCOP proteins and identified 354 domain-interaction sites. 14 interface properties were calculated from amino acid and secondary structure composition and then used to generate a set of association rules characterizing these domain-interaction sites employing the APRIORI algorithm. Our results regarding the classification of PPI types based on a set of discovered association rules shows that the discriminative ability of association rules can significantly impact on the prediction power of classification models. We also showed that the accuracy of the classification can be improved through the use of structural domain information and also the use of secondary structure content. Conclusion: The advantage of our approach is that we can extract biologically significant information from the interpretation of the discovered association rules in terms of understandability and interpretability of rules. A web application based on our method can be found at http://bioinfo.ssu.ac.kr/~shpark/picasso/SHP was supported by the Korea Research Foundation Grant funded by the Korean Government(KRF-2005-214-E00050). JAR has been
supported by the Programme Alβan, the European Union Programme of High level Scholarships for Latin America, scholarship E04D034854CL. SK was supported by Soongsil University Research Fund
Envolvimento dos canais de potássio medular no efeito antiedematogênico da morfina administrada por via intratecal
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Farmacologia, Florianópolis, 2011A medula espinhal tem sido considerada um potencial sítio de modulação da inflamação e não apenas uma simples linha de transmissão da periferia para o cérebro. O reflexo da raiz dorsal (RRD) caracterizado pela atividade antidrômica nas fibras aferentes primárias pode interferir na inflamação periférica. Neste contexto, estudos anteriores demonstraram que a administração de morfina intratecal resulta em efeito antiedematogênico com envolvimento da via L-arginina/ Óxido Nítrico (NO)/ GMPc. O presente estudo estende esses resultados a fim de avaliar a hipótese do envolvimento dos canais de potássio em tal efeito. As drogas foram injetadas na intratecal de ratos Wistar machos em volume de 20 L, 30 min antes do estímulo edematogênico da carragenina (CG -150 g/50 l, subcutâneo) na pata posterior direita. A avaliação do edema foi medido como aumento de volume da pata (ml) e pelo extravasamento plasmático pelo corante azul de Evans. A migração de neutrófilos foi avaliada indiretamente pelo ensaio da mieloperoxidase (MPO). A quantificação do infiltrado inflamatório e congestão vascular ocorreu através da análise histológica. A administração de morfina (37 nmol) inibiu o edema inflamatório, o extravasamento do corante azul de Evans e a congestão vascular. Entretanto, não teve efeito na atividade da MPO e no infiltrado inflamatório. A coadministração da morfina (37 nmol) com 4-aminopiridina (10 nmol), Glibenclamida (5 nmol) e Dequalinium (10 pmol) reverteu o efeito inicial da morfina. Estes resultados suportam a idéia de que a morfina pode atuar em receptores opióides medular produzindo efeito antiedematogênico, mediado pela abertura de canais de potássio, via NO/GMPc
Maternal methadone use in pregnancy : factors associated with the development of neonatal abstinence syndrome and implications for healthcare resources
No full textThe objectives of this study were to investigate factors associated with the development of neonatal abstinence syndrome (NAS) and to assess the implications for healthcare resources of infants born to drug-misusing women. Design. Retrospective cohort study from 1 January 2004 to 31 December 2006. Setting. Inner-city maternity hospital providing dedicated multidisciplinary care to drug-misusing women. Four hundred and fifty singleton pregnancies of drugmisusing women prescribed substitute methadone in pregnancy. Development of NAS and duration of infant hospital stay. 45.5% of infants developed NAS requiring pharmacological treatment. The odds ratio of the infant developing NAS was independently related to prescribed maternal methadone dose rather than associated polydrug misuse. Breastfeeding was associated with reduced odds of requiring treatment for NAS (OR 0.55, 95% CI 0.34-0.88). Preterm birth did not influence the odds of the infant receiving treatment for NAS. 48.4% infants were admitted to the neonatal unit (NNU) 40% of these primarily for treatment of NAS. The median total hospital stay for all infants was 10 days (interquartile range 7-17 days). Infants born to methadone-prescribed drug-misusing mothers represented 2.9% of hospital births, but used 18.2% of NNU cot days. Higher maternal methadone dose is associated with a higher incidence of NAS. Pregnant drug-misusing women should be encouraged and supported to breastfeed. Their infants are extremely vulnerable and draw heavily on healthcare resources
Efeito da quercetina no extravasamento plasmático induzido pela substância P na dura-máter, cerebelo, bulbo olfatório e córtex de ratos
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas.Investigação dos efeitos da quercetina (30 mg/Kg v.o.) no extravasamento plasmático induzido pela substância P (10 nmol/Kg i.v.) em diversos tecidos do sistema nervoso central (cerebelo, bulbo olfatório e córtex) e dura-máter de ratos além dos efeitos da inibição da endopeptidase neutra pelo fosforamidon (2,5 µg/Kg i.v.) e da enzima conversora da angiotensina pelo captopril (10 nmol/Kg i.v.) nos mesmos tecidos. A substância P aumentou o extravasamento plasmático de modo dose relacionada na dura-máter, não alterando os outros tecidos. A inibição farmacológica seletiva da endopeptidase neutra e da enzima conversora da angiotensina com fosforamidon e captopril respectivamente aumentaram o extravasamento plasmático induzido pela substância P na dura-máter de ratos. O pré-tratamento isolado com quercetina (30mg/Kg, v.o) aumentou o extravasamento plasmático em relação ao controle em todos os tecidos quando administrada isoladamente e potencializou o extravasamento plasmático induzido pela substância P na dura-máter, tecido onde há inervação peptidérgica o que coincide com a modulação da endopeptidase neutra e da enzima conversora da angiotensina
Caracterização do receptor e dos mecanismos relacionados a resposta inflamatoria induzida pela des-Arg9-bradicinina no modelo murino de pleurisia
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciencias BiologicasCaracterização do receptor B1, e dos mecanismos relacionados à resposta inflamatória induzida pela des-Arg9-bradicinina na cavidade pleural de camundongos. Os resultados mostram que a des-Arg9-bradicinina causa resposta inflamatória caracterizada por um pico de 1h para o extravasamento e um pico de 4 h para a migração de neutrófilos para a cavidade pleural dos animais. Nesta resposta inflamatória estão envolvidos vários mediadores inflamatórios, entre eles, as taquicininas, o CGRP, o óxido nítrico, e produtos das ciclooxigenases. O tratamento prévio dos animais com LPS não aumentou a expressão dos receptores B1 na cavidade pleural de camundongos, sugerindo que estes receptores estão presentes na sua forma constitutiva
NQO2 is a reactive oxygen species generating off-target for acetaminophen
Get PDFThe analgesic and antipyretic compound acetaminophen (paracetamol) is one of the most used drugs worldwide. Acetaminophen overdose is also the most common cause for acute liver toxicity. Here we show that acetaminophen and many structurally related compounds bind quinone reductase 2 (NQO2) in vitro and in live cells, establishing NQO2 as a novel off-target. NQO2 modulates the levels of acetaminophen derived reactive oxygen species, more specifically superoxide anions, in cultured cells. In humans, NQO2 is highly expressed in liver and kidney, the main sites of acetaminophen toxicity. We suggest that NQO2 mediated superoxide production may function as a novel mechanism augmenting acetaminophen toxicity.</p
Oral Conditions In Very Low And Extremely Low Birth Weight Children
No full textPurpose: The purpose of this study was to evaluate the oral conditions in very low birth weight (VLBW) and extremely low birth weight (ELBW) children. Methods: Fifty-two VLBW and ELBW 2- to 4-year-old children (V/ELBW group) were evaluated and compared with 52 normal birth weight children (NBW group). The oral examination was performed in accordance with World Health Organization criteria. The parents/guardians answered a structural questionnaire about the children's feeding and oral habits. The prenatal, natal, and neonatal information was obtained from the hospital files. Results: The enamel defects, such as demarcated opacity and hypoplasia, had a significant association with prematurity and VLBW and ELBW. The breast-feeding and bruxism were more often in the NBW group, whereas nocturnal bottle-feeding, pacifier use, and high frequency of oral hygiene were associated with prematurity and lower birth-weight. V/ELBW group children were at a 12.5 and 6.6 times greater risk of presenting demarcated opacity and hypoplasia, respectively, than NBW children. The risk of presenting nocturnal bottle-feeding, good oral hygiene, and enamel defects was significantly higher for the V/ELBW group. 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