4,007 research outputs found
Insights into the Musa genome: Syntenic relationships to rice and between Musa species
Get PDFAbstract Background Musa species (Zingiberaceae, Zingiberales) including bananas and plantains are collectively the fourth most important crop in developing countries. Knowledge concerning Musa genome structure and the origin of distinct cultivars has greatly increased over the last few years. Until now, however, no large-scale analyses of Musa genomic sequence have been conducted. This study compares genomic sequence in two Musa species with orthologous regions in the rice genome. Results We produced 1.4 Mb of Musa sequence from 13 BAC clones, annotated and analyzed them along with 4 previously sequenced BACs. The 443 predicted genes revealed that Zingiberales genes share GC content and distribution characteristics with eudicot and Poaceae genomes. Comparison with rice revealed microsynteny regions that have persisted since the divergence of the Commelinid orders Poales and Zingiberales at least 117 Mya. The previously hypothesized large-scale duplication event in the common ancestor of major cereal lineages within the Poaceae was verified. The divergence time distributions for Musa-Zingiber (Zingiberaceae, Zingiberales) orthologs and paralogs provide strong evidence for a large-scale duplication event in the Musa lineage after its divergence from the Zingiberaceae approximately 61 Mya. Comparisons of genomic regions from M. acuminata and M. balbisiana revealed highly conserved genome structure, and indicated that these genomes diverged circa 4.6 Mya. Conclusion These results point to the utility of comparative analyses between distantly-related monocot species such as rice and Musa for improving our understanding of monocot genome evolution. Sequencing the genome of M. acuminata would provide a strong foundation for comparative genomics in the monocots. In addition a genome sequence would aid genomic and genetic analyses of cultivated Musa polyploid genotypes in research aimed at localizing and cloning genes controlling important agronomic traits for breeding purposes.</p
Analysis of non-TIR NBS-LRR resistance gene analogs in <it>Musa acuminata </it>Colla: Isolation, RFLP marker development, and physical mapping
Get PDFAbstract Background Many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. Fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (R-genes). The largest known family of plant R-genes encode proteins with nucleotide-binding site (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for isolation of candidate genes in banana which may be involved in plant defence. Results A computational strategy was developed for unbiased conserved motif discovery in NBS and LRR domains in R-genes and homologues in monocotyledonous plant species. Degenerate PCR primers targeting conserved motifs were tested on the wild cultivar Musa acuminata subsp. burmannicoides, var. Calcutta 4, which is resistant to a number of fungal pathogens and nematodes. One hundred and seventy four resistance gene analogs (RGAs) were amplified and assembled into 52 contiguous sequences. Motifs present were typical of the non-TIR NBS-LRR RGA subfamily. A phylogenetic analysis of deduced amino-acid sequences for 33 RGAs with contiguous open reading frames (ORFs), together with RGAs from Arabidopsis thaliana and Oryza sativa, grouped most Musa RGAs within monocotyledon-specific clades. RFLP-RGA markers were developed, with 12 displaying distinct polymorphisms in parentals and F1 progeny of a diploid M. acuminata mapping population. Eighty eight BAC clones were identified in M. acuminata Calcutta 4, M. acuminata Grande Naine, and M. balbisiana Pisang Klutuk Wulung BAC libraries when hybridized to two RGA probes. Multiple copy RGAs were common within BAC clones, potentially representing variation reservoirs for evolution of new R-gene specificities. Conclusion This is the first large scale analysis of NBS-LRR RGAs in M. acuminata Calcutta 4. Contig sequences were deposited in GenBank and assigned numbers ER935972 – ER936023. RGA sequences and isolated BACs are a valuable resource for R-gene discovery, and in future applications will provide insight into the organization and evolution of NBS-LRR R-genes in the Musa A and B genome. The developed RFLP-RGA markers are applicable for genetic map development and marker assisted selection for defined traits such as pest and disease resistance.</p
POSLOVNI PLAN „PČELARSTVO MUSA“
No full textU razvijenim zemljama u širokoj su upotrebi pčelinji proizvodi, kao i zemljama u
razvoju. Pčelarstvo je vrsta djelatnosti kojoj je potrebno mnogo pažnje i vremena za posvetiti
se. Iz ljubavi prema prirodi, i pčelama ne može roditi ništa loše, stoga je pčelarstvo pravi poziv
za ljubitelje prirode i njihovih oprašivača, tj. pčela. Poslovno planiranje je utvrđivanje ciljeva
poduzeća i njegovih rezultata pomoću kojih se mogu ostvariti zadani ciljevi. Poslovni plan je
dokument koji pobliže pojašnjava ulaganja u poduzetnički pothvat s ocjenom predviđenih
učinaka i mogućih rješenja za različite situacije što ih donosi buduće vrijeme. Poslovni plan
prikazuje sliku trenutnog i budućeg poslovanja na temelju koje poduzetnik/investitor donosi
odluku o svom budućem djelovanju. Predmet ovog poslovnog plana je ulaganje u otvorenje
poslovnog prostora novostvorenog obiteljskog poljoprivrednog poduzeća od strane obitelji
Musa, u Đakovu. Konkurencija na tržištu je velika, te je zbog toga potrebno ulaganje kako bi
se smanjio trošak proizvodnje, a povećalo zadovoljstvo i privlačenje novih kupaca. Investicija
bi iznosila 470.000,00 kn i nju bi se financiralo 20 % iz bankarskog kredita i 80 % vlastitim
sredstvima dodijeljenim bespovratnim sredstvima EU putem Programa ruralnog razvoja RH.
Ovim poslovnim planom pokazati će se da je investicija opravdana jer će se proces proizvodnje
poboljšati i olakšati, kao i odnos i poslovanje s krajnjim kupcima/potrošačima
Identification and expression of the Pseudomonas syringae pv. aptata hrpZPsa gene which encodes an harpin elicitor
No full textA sequence homologous to an internal fragment 0.75 kb BstXI of the Pseudomonas syringae pv. syringae hrpZ gene was identified in Pseudomonas syringae pv. aptata NCPPB 2664, the causal agent of bacterial blight in sugar beet, lettuce and other plants, and in E. coli DH10B (pCCP1069) containing the P. syringae pv. aptata hrp gene cluster. PCR with oligonucleotides, based on the hrpZPss gene and used as primers with the total genomic DNA of P. syringae pv. aptata, amplified a 1 kb fragment that hybridized with the probe in highly stringent conditions. The amplicon was cloned into the pGEM® plasmid vector, amplified in E. coli DH5α and sequenced. The sequence showed 95%, 83% and 61% identity with those of hrpZPss, hrpZPsg and hrpZPst genes encoding the harpins of the P. syringae pv. syringae, glycinea and tomato, respectively. The amplicon was cloned into the pMAL® expression system. The expressed protein, fused with maltose-binding protein, was cleaved with a specific protease factor Xa, and purified using affinity chromatography. On the basis of the amino acid sequence and its ability to induce HR in tobacco leaves, it was identified as a P. syringae pv. aptata harpin
Taxa de multiplicação de mudas micropropagadas de bananeira cv. Grande Naine e cv. Prata Catarina influenciada pela fase de estabelecimento de cultura
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2015.A bananeira é a segunda fruteira mais cultivada do mundo, sendo o Brasil o quinto no ranking, com 6,8% da produção mundial de banana. No ano de 2014, a estimativa da área de produção brasileira foi de 490,1 mil hectares, produção de 7,18 milhões de toneladas de frutas (EPAGRI/CEPA, 2014). Nas últimas décadas, a produção se expandiu na maioria dos países produtores; passou de 35 milhões para 102 milhões de toneladas entre as safras 1978 e de 2012, parte deste aumento de produção se deve ao avanço tecnológico, principalmente devido disponibilidade de material genético diversificado e de mudas com valor agregado, principalmente qualidade fitossanitária obtida através de técnicas biotecnológicas. Apesar das biofábricas terem protocolos de micropropagação bem sucedidos, os mesmos não são suficientes para atenderem a demanda com qualidade e quantidade de mudas. Nos últimos anos os sistemas de propagação, em larga escala, não atingiu o número de mudas desejadas, mesmo com a utilização de biorreatores de imersão temporária, o qual tem sido associado ao aumento dos riscos de surgimento de variantes somaclonais. O presente trabalho teve por objetivo mostrar o comportamento das culturas in vitro de Musa acuminata (AAA) cv. Grande Naine e de Musa acuminata x Musa balbisiana (AAB) cv. Prata Catarina, submetidas a alterações no protocolo de micropropagação, comumente utilizado em biofábricas, visando obter maior taxa de multiplicação. Utilizando-se de material vegetal proveniente da coleção da EPAGRI/EEI, o trabalho foi dividido em duas etapas: fase de estabelecimento de cultura e fase de multiplicação. Na fase de estabelecimento, utilizou-se como meio base a formulação MS, acrescida de 1 mg.L-1 de BAP e 1 mg.L-1 de ANA, tendo como variáveis nos tratamentos propostos: aumento do tempo de cultivo in vitro; alterações de concentração de sais e adição de diferentes fitormônios. Para a fase de multiplicação utilizou-se as culturas provenientes da fase de estabelecimento, em cinco subcultivos de 30 dias, utilizando a formulação do meio MS e tendo como variáveis: alteração no estado físico (sólido/liquido), adição de BAP e adição de BAP/ANA. O meio MS, acrescido de 2,5 mg.L-1 de BAP promoveu a maior taxa de multiplicação, em ambas as cultivares estudadas. As melhores taxas de multiplicação quando consideradas fases de estabelecimento e multiplicação, para cultivar Grande Naine, foram obtidas em meio MS, acrescido de 1 mg.L-1 de BAP e 1 mg.L-1 de ANA por 90 dias em estabelecimento de cultura, utilizando na sequencia meio MS com 2,5 mg.L-1 de BAP para multiplicação em 5 subcultivos de 30dias consecutivos, alcançando taxas de multiplicação de 356 brotos por explante inicial, superior em 5 vezes ao obtido no tratamento similar ao praticado em biofábricas. Quanto a cultivar Prata Catarina o desempenho obtido não foi superior ao alcançado em biofábricas e os tratamentos não apresentaram diferenças estatísticas significativas. Foi utilizado meio MS com 50% dos sais da formulação para prover crescimento e enraizamento. Na fase de aclimatização, o método utilizado foi eficiente gerando número total de mudas para as cultivares Grande Naine e Prata Catarina de 8.995 e 3.911, respectivamente. O estudo indicou que alterações na fase de estabelecimento de cultura resultam em aumento da taxa de multiplicação para o cv. Grande Naine, enquanto que para o cv. Prata Catarina não apresenta efeito significativo.Abstract : The Banana is the second most cultivated fruit tree in the world, and Brazil is the fifth in the ranking, with 6.8% of the world production. In the year 2014, the estimate of Brazilian production area was 490,100 hectares, producing 7.18 million tons of fruit (EPAGRI/CEPA, 2014). In the last decades, production has expanded in most producing countries, from 35 million tons in 1978 to 102 million tons in 2012, part of this increase in production is due to technological advances related to the availability of more genetically diverse germplasm and of seedlings with higher sanitary quality obtained through biotechnological techniques. Despite successful micropropagation protocols adopted by biofactories , they are not sufficient to meet the demand with quality and quantity of seedlings. In recent years propagation systems on a large scale has not reached the desired number of plants, even with the use of temporary immersion bioreactor, which has been associated with increased risks of onset of somaclonal variants. This study aimed to show the behavior of in vitro cultures of Musa acuminata (AAA) cv. Grande Naine and of Musa acuminata x Musa balbisiana (AAB) cv. Prata Catarina when changes in the micropropagation protocol were made compared to the traditional protocols, for an increase in the micropropagarion rate. The work was divided in two phases, namely culture establishment and culture multiplication. The explants used came from a field collection established at Epagri/Itajaí Research Station. In the phase of establishment of culture, MS base medium plus 1 mg.L-1 BAP and 1 mg.L-1 NAA was used, and there was an increase in the in vitro culture time, as well as changes in the salt concentration and in the phytohormones used. For the multiplication phase, materials generated from five subcultures of 30 days each were used, and changes in the physical state (solid/liquid) and in the concentration of cytokinin (BAP) and cytokinin/auxin (NAA). The treatment that consisted of MS medium plus 2.5 mg.L-1 BAP promotedthe highest multiplication rate in both cultivars. For the cultivar Grande Naine, the highest multiplication rates for both the establishment and the multiplication phases were obtained when MS medium plus 1 mg.L-1 de BAP and 1 mg.L-1 de NAA for 90 establishing days in culture was used, followed by multiplication in MS medium with 2.5 mg.L-1 BAP in five subcultures of 30 days each; the multiplication rates obtained were 356 shoots per initial explant, five times higher than that obtained in a similar treatment adopted by biofactories. However, for the cultivar Prata Catarina, the performance of the treatments was not statistically different from the performance of the traditional protocols adopted by biofactories. Half-strength MS medium was used to promote growth and root development. The method adopted in the acclimatization stage was efficient, generating 8,995 and 3,911 plants for the cultivars Grande Naine and Prata Catarina, respectively. The study indicated that change in culture establishment phase result in increased multiplication rate for the cv. Grand Naine, while for cv. Prata Catarina has no significant effect
An Overview on the Healing Potentials of Musa sapientm (Banana) in the Treatment of Peptic Ulcer Disease
Get PDFGastric ulcer is a localized area of erosion in the stomach lining, resulting in abdominal pain, possible bleeding, and other gastrointestinal symptoms. The most common cause of gastric ulcer is Non-steroidal anti-inflammatory drugs (NSAIDs) and a stomach infection associated with the Helicobacter pylori (H. pylori) bacteria. The management of peptic ulcer disease and its complications remain a surgical challenge. Therefore, the evolution of newly discovered antiulcer drugs from medicinal plants is an attractive area because several chemicals with anti-ulcer effects have been found in these plants, and they have shown a promising potential in the treatment of the disease. Musa sapientum (Family: Musaceae), known as banana, is a familiar tropical fruit, a treelike perennial herb that grows 5-9 m in height, with a tuberous rhizome, hard, long pseudo-stem. The inflorescence is big with a reddish-brown bract, and it is eaten as vegetables, and the ripe fruits are sweet. Some studies reported that pectin and phosphatidylcholine in green banana strengthen the mucous-phospholipid layer that protects the gastric mucosa. Other studies highlight that leucocyanidin, a natural flavonoid from the unripe banana (Musa sapientum) pulp, protects the gastric mucosa from erosions. The present study aims at reviewing the relevant studies on bioactive compounds in Musa sapientum with their healing potentials in gastric ulcer diseases
Kompozitni materijali u građevinarstvu
No full textRazvoj u raznim granama tehnike, a tako i građevinarstva. Građevinarstvo je primijenjena znanost, najstarija i ujedno najznačajnija grana tehnike koja se bavi tehničko tehnološkim aspektima izgradnje objekata kao i proizvodnjom građevnih materijala, poluproizvoda i proizvoda. Građevinarstvo u Hrvatskoj ima višestoljetnu tradiciju koju nam predočavaju brojni dvorci, crkve, javne zgrade i mnogi drugi objekti koji su vješto izgrađeni od strane njihovih graditelja. Cilj razvitka je težiti razvoju što novijim, boljim i jeftinijim idejama koje se nakon toga pretvaraju u proizvode. Niti materijali koji se koriste u izgradnji nisu izuzetak tome. Od raznih materijala koji su se koristili u prošlosti kao što su drvo, glina, raznih metala do današnjih mješavina više materijala koju nazivamo kompozitima. Kompozitni materijali nam omogućuju bolje karakteristike u svrhu izgradnje što kvalitetnijeg objekta, a da
budu u optimalnim financijskim granicama. Pojavom i razvitkom kompozita smanjuje se masa raznih elemenata gradnje, produljuje vijek trajanja kao i njihova svojstva, čini elemente otpornije na vremenske uvijete, omogućava korištenje zelenije tehnologije što je iznimno bitno i za očuvanje okoliša. Kompozitni materijali imaju svoju budućnost zbog toga što su korišteni u brojnim drugim područjima tehnike te njihov razvitak neosporan za razvitak i stvaranje kvalitetnijih proizvoda
Musical elements in As m's Divan
No full textMüzik ve iir, insan n duygu ve dü üncelerini ifade etme vas talar ndan olmalar nedeniyle zaman zaman yollar kesi en iki sanat dal d r. Müzik, klâsik Türk iirinde âhengi sa layan unsurlardan biri olarak da her zaman var olmu tur. Bunun yan nda müstakil bir ilim dal olarak ise birçok iire konu olmu tur. Yine klâsik Türk mûsikîsine ait terimler, bazen ortam tasvîrinde, bazen memduh övgüsünde, bazen de â n içinde bulundu u psikolojik durumu ifade etmede yard mc birer unsur olarak dîvân airleri taraf ndan s kl kla kullan lm t r. Bu çal mada da klâsik Türk mûsikîsinin, on yedinci yüzy l airlerinden olan Âs m (öl. 1710) n dîvân üzerindeki yans malar incelenmeye çal lm t r. airin mûsikî unsurlar n , eserinin hangi bölümlerinde ve hangi gerekçelerle kulland n n ortaya ç kar lmas amaçlanm t r.Because of being means of expression of humanbeings emotions and thoughts, music and poetry are occasionally two intersecting paths of art. Music has always been in the classical Turkish poetry as one of the elements providing harmony. Besides, as an independent branch of science, it has been a subject to many poems. Terms belonging to the classical Turkish music have often been used as one of the auxiliary elements by divan poets; sometimes in description of environment, sometimes in the compliment of the praised, sometimes in expressing the psychological situation of lover, too. In this study, it has been endeavored to examine the reflections of the classical Turkish music upon As ms (d. 1710) Divan, who is one of the poets from the seventeenth century. It has been aimed at revealing in which sections and by which realities musical elements of the poet are used
Man up: stories of Parvez and Muna
No full textA collection of linked comic short stories about a young Muslim couple, tracing their relationship from their traditional courtship to the early parenthood.M.F.A.by Musa Syee
Bernard Pannagl's musa panagaea
No full textBernard Pannag1 (1666-1734, from 1684 member of Societas Jesu) was a gymnaSIUm teacher, predicator and 1ibrarian in the C1ementinum college in Prague. On1y five books of him are known, all of them in latin. One of these is Musa panagaea (edited probab1y in J anuary 1729), a collection of his schoo1 dramatic works. We bring an edition of the first part of the Musa panagaea, name1y the p1ays Joannes Nepomucenus, Divus Joannes Baptista and Romulus, with an introduction to the latin schoo1 drama in the 17. and 18. century and with informations about the author. There are no standard rules for the editions of the latin texts from 17. and 18. century, just some recommendations. The interpunction of that age in Bohemia hasn't been described and explained in detail s yet, and iťs different from the interpunction, which is used in the czech language (and in the enghsh as well) today; in our edition we use the czech modem interpunction so as the text can be easily read by modem readers. In the transcription of the words we respect the specific aspects of the modem latin (as we know especially from the works of Bohuslav Balbín) and we don't rewrite them in the c1assical latin way. Using of accents in the Musa panagaea is regular and we describe some rules, which we respect in the transcription of the text, but these..
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