74 research outputs found
2006 Author Recognition Bibliography
No full texthttps://scholarworks.gvsu.edu/authorrecognition/1008/thumbnail.jp
Armament and Society in the Mirror of the Avar Archaelogy The Transdanubia-Phenomenon Revisited
No full textOne of the most significant problems of the A var archaeology is the question of
Germanic (mainly Gepidic) continuity in Transdanubia. In my paper I would like to
make some comments on the so-called Transdanubia-phenomenon of the Early A var
Carpathian Basin based on the analysis of weapon-combinations found in six
cemeteries of Eastern Transdanubia. I intend to answer the following questions: I.
How far the weapon-combinations of the East-Transdanubian cemeteries of the early
Avar Period (568-650) are identical or similar to the general picture of Avar armament
drawn by contemporary cemeteries? 2. Are the weapon-combinations or armament of
these cemeteries similar to that of the earlier Gepidic and Langobardic sites from the
early 6th centuries or to the contemporary Germanic (Alemannic, Frank or Bavarian)
cemeteries of the present-day Germany?
As a result, the early A var cemeteries of Transdanubia are characterized by the
relatively high number of close-combat weapons compared to other sites of the Avar
Khaganate. However, comparing to Merovingian sites the burials containing only
close-combat weapons are very low and in most of the cases the weapon-combinations
characteristic to this culture is missing
Catalytic mechanism of alpha-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling.
No full textEnzymatic synthesis and hydrolysis of nucleoside phosphate compounds play a key role in various biological pathways, like signal transduction, DNA synthesis and metabolism. Although these processes have been studied extensively, numerous key issues regarding the chemical pathway and atomic movements remain open for many enzymatic reactions. Here, using the Mason-Pfizer monkey retrovirus dUTPase, we study the dUTPase-catalyzed hydrolysis of dUTP, an incorrect DNA building block, to elaborate the mechanistic details at high resolution. Combining mass spectrometry analysis of the dUTPase-catalyzed reaction carried out in and quantum mechanics/molecular mechanics (QM/MM) simulation, we show that the nucleophilic attack occurs at the alpha-phosphate site. Phosphorus-31 NMR spectroscopy (31P-NMR) analysis confirms the site of attack and shows the capability of dUTPase to cleave the dUTP analogue alpha,beta-imido-dUTP, containing the imido linkage usually regarded to be non-hydrolyzable. We present numerous X-ray crystal structures of distinct dUTPase and nucleoside phosphate complexes, which report on the progress of the chemical reaction along the reaction coordinate. The presently used combination of diverse structural methods reveals details of the nucleophilic attack and identifies a novel enzyme-product complex structure
D. Carlos: the fictional double reflected in the historical truth
No full textInserida no projeto Autor por Autor, que consiste na análise de textos teatrais cujo tema e/ou motivo trata(m) da História Portuguesa, esta dissertação dedicou-se à análise e interpretação das peças Pátria, de Guerra Junqueiro, e D. Carlos: drama em verso, de Teixeira Pascoaes, que trazem uma personagem ficcional criada a partir de D. Carlos, rei de Portugal entre os anos de 1889 e 1908. No diálogo travado entre a Ficção e a História, buscou-se a visão que Guerra Junqueiro e Teixeira Pascoaes tiveram do papel desempenhado por D. Carlos, rei de Portugal, durante a agonia da monarquia portuguesa, às vésperas do nascimento e fim da Primeira República. A pesquisa em torno da personagem histórico-real procura responder quem foi D. Carlos na representação de historiadores, com o objetivo de, depois de analisadas as peças, traçar paralelos entre as personagens ficcionais criadas à luz do D. Carlos histórico-real. Por meio desta comparação é possível perceber a escolha das características elencadas por cada escritor para compor as personagens ficcionais em decorrência da mensagem alegórica que cada texto ficcional possui, examinando em que medida a História surge como uma construção que muito deve à verossimilhança e interpretação subjetiva da Ficção. As personagens ficcionais, que representam o rei em cada uma das peças, parecem antagônicas entre si. A explicação para tal disparidade está intrinsecamente ligada ao drama histórico definido por Almeida Garrett durante o Romantismo Português, motivo de essa definição ter sido utilizada como ponto de partida para a metodologia escolhida para a análise das peças, posto que o exame dos textos literários encontrou uma releitura didática do presente à luz de alegorias criadas com a História de Portugal e por meio da tradição das escolas literárias nas quais se inserem, Decadentismo-Simbolismo (Pátria) e Saudosismo (D. Carlos: drama em verso). Para que tal caráter didático-elucidativo fosse revelado, fizeram-se necessárias as análises dos períodos históricos de elaboração e publicação de Pátria (1896) e D. Carlos: drama em verso (1918-1925). A análise da personagem foi fundamentalmente baseada nos estudos de Renata Pallottini em seu livro Dramaturgia: a construção da personagem, de onde foram elencados três pontos a serem estudados para compreender a construção das personagens que representam D. Carlos nas peças analisadas. Os três enfoques revelaram-se intrinsecamente interligados e determinantes para a escolha dos atributos da personagem histórico-real, utilizados para compor as personagens histórico-ficcionais que representam o rei em Pátria e D. Carlos. Os levantamentos históricos, as análises das peças e das personagens conduziram a dissertação à conclusão de que, em ambos os casos, D. Carlos era a personagem histórica mais emblemática para a construção da mensagem de cada peça, por ser ele o representante da monarquia nos períodos históricos que compuseram a unidade de ação, tempo presente, das peças aqui analisadas.Inserted into the Project Author by Author, which is the analysis of theatrical texts whose theme and/or reason tells about Portuguese history, this work is dedicated to the analysis and interpretation of the books Pátria, from Guerra Junqueiro, and D. Carlos: drama em verso, from Teixeira de Pascoaes, who brings both a fictional character created from D. Carlos, King of Portugal, between the years 1889 and 1908. By the dialogue between fiction and history, we sought the view that Guerra Junqueiro and Teixeira de Pascoaes had for the role played by D. Carlos, King of Portugal, at the Portuguese monarchy decadence period, on the eve of the First Republic. Looking through the historical research carried out around the real historical character, we answer who was D. Carlos in the mental representation of historians, and then by analyzing each play, in order to draw parallels between the fictional characters created and the real historical D. Carlos, we justify the choice of the characteristics listed by both writers through the allegorical message of each fictional plays, examining the extent of the history construction that owes much to the likelihood and subjective interpretation of fiction. The explanation for the disparity between the allegorical messages and fictional characters of each piece, appears intrinsically linked to the historical drama defined by Almeida Garrett during Portuguese Romanticism, link that have been the starting point for the chosen methodology to our books analysis, becouse the examination of each literary texts founds a didactic retelling of the allegories created with the Portugal history and through the tradition of literary schools to which each play belong, Decadentism-Symbolism (Pátria) and Saudosismo (D . Carlos: drama em verso). To reveal this didactic and instructive character, it was necessary to analyzes the historical periods of preparation and publication of Pátria (1896) and D. Carlos: drama em verso (1918-1925). The characters analysis was primarily based on Renatas Pallottini studies in her book Dramaturgia: a construção da personagem, from which were listed three points to be studied to understand the construction of the characters representing D. Carlos on the analyzed parts. The three approaches have proven to be intrinsically linked and decisive for the choice of the real historical character atributes, used to compose the historical-fictionals characters that represent the King in Pátria and D. Carlos. Historical surveys, and the character analysis led us to the conclusion that, in both cases, D. Carlos was the most emblematic historical character to the construction of each piece messages, because he was the simbol of the monarchy at the historical periods that built up the unity of action and present time, by the documents analyzed here
Age-related Differences in Implicit Sequence Learning and Consolidation across the Human Life Span: Implications for the Functioning of the Fronto-Striatal Circuitry
No full textImplicit sequence learning occurs when information is acquired from an environment of complex stimuli without conscious access either to what was learned or to the fact that learning occurred. In everyday life, this learning mechanism is crucial for adapting to the environment and for predicting events unconsciously. Despite the growing interest in implicit learning in the past decades, there has been relatively little research on life-long development of implicit sequence learning and on offline processing of implicitly learned information (i.e., consolidation). Here, we present three studies investigating these issues. In Experiment I, we investigated implicit sequence learning from 4 to 85 years of age and found a marked decrease in learning performance - measured by raw reaction time (RT) – around age of 12. This decrement can be explained by a competition between two fundamentally different forms of learning (model-free and model-based) suggesting that after adolescence frontal lobe-mediated model-based learning has larger effect on the expression of implicit sequence knowledge, while before adolescence basal ganglia-dependent model-free learning is more influencing. As a growing body of data has shown that frontal lobe-mediated processes are disrupted in hypnosis, we tested this assumption in Experiment II by comparing implicit sequence learning in hypnosis and in waking alert state. We found that hypnosis boosted sequence learning in young adults. In addition, this boosting effect was mediated by frontal lobe related executive functions. Finally, we investigated consolidation of implicit sequence knowledge in young and elderly adults after 12-, 24-hour or a 1-week delay period in order to determine age-related differences not only in online learning, but also in offline processing of the learned material (Experiment III). We found that consolidation is not a single process, rather there are multiple mechanisms (e.g., sequence-specific, general skill learning) which are differentially affected by aging and the course of time. Our results contribute not only to the better understanding of learning on a behavioral level, but also to understanding the age-related changes in brain plasticity in healthy participants across the human life span. In addition, these findings can help better understand neurodevelopmental (e.g., autism, dyslexia), neurodegenerative (e.g., Parkinson’s Disease, Huntington’s Disease) and age-related disorders where related brain structures are affected. Finally, our findings can lead to the development of more effective diagnostic tools, training methods and rehabilitation programs
SPORTS, EXERCISE ONLINE? THE OPINION OF SPORT MAJORS ON THE NON-ATTENDANCE TUITION
No full textAim of the study: The aim of our research is to map out the new aspect of life, in one section of higher education, created by the epidemiological situation, to get to know the methodics used by the teachers of the two sport science institutes (ESI, STI) in our faculty, and get to know our student\u27s opinion on the topic. Methods: We worked with our own questionnaire, which includes the following groups of questions: demographic data, questions about the students\u27 availability of tools, students\u27 opinions about the requirements, communication with the instructor, the completion of the courses, changes in their own learning habits and their physical and practical preparation capabilities. The members of sample was studied at the Eötvös Loránd University PPK in Szombathely and Budapest by students majoring in sport field. Results: No difficulties were reported by our students, 90% had adequate tools. Their workload was much higher than in previous periods, which was reflected in a strong increase in time spent with studying (p <0.01). The lessons, tasks and dissertations carried out in the online learning space were a bigger challenge for the lecturers, based on the opinion of our students, the education did not seem more exciting or creative (2.8 on a 6-point scale). Opinions about the educational platforms used were evenly distributed, they did not find it difficult to navigate between the different surfaces (3.66 on a scale of 6.).Conclusion: The virtue of problem solving could be practiced in recent times. It is advisable to incorporate the experience of the recent period into the solutions of the important tasks aheadAim of the study: The aim of our research is to map out the new aspect of life, in one section of higher education, created by the epidemiological situation, to get to know the methodics used by the teachers of the two sport science institutes (ESI, STI) in our faculty, and get to know our student\u27s opinion on the topic. Methods: We worked with our own questionnaire, which includes the following groups of questions: demographic data, questions about the students\u27 availability of tools, students\u27 opinions about the requirements, communication with the instructor, the completion of the courses, changes in their own learning habits and their physical and practical preparation capabilities. The members of sample was studied at the Eötvös Loránd University PPK in Szombathely and Budapest by students majoring in sport field. Results: No difficulties were reported by our students, 90% had adequate tools. Their workload was much higher than in previous periods, which was reflected in a strong increase in time spent with studying (p <0.01). The lessons, tasks and dissertations carried out in the online learning space were a bigger challenge for the lecturers, based on the opinion of our students, the education did not seem more exciting or creative (2.8 on a 6-point scale). Opinions about the educational platforms used were evenly distributed, they did not find it difficult to navigate between the different surfaces (3.66 on a scale of 6.).Conclusion: The virtue of problem solving could be practiced in recent times. It is advisable to incorporate the experience of the recent period into the solutions of the important tasks ahea
Biophysical and Cell Biological Studies Characterizing the Vertebrate Iron Exporter Ferroportin
No full textMammalian iron homeostasis is maintained by an intricate network of diverse proteins that constantly survey systemic iron levels and carefully regulate the uptake of iron from the diet. Control of this uptake is critically important because once iron is absorbed, mammals have no regulated mechanism for its removal. The portal through which iron enters the body is ferroportin, a multipass membrane protein expressed on the basolateral membrane of epithelial cells in the duodenum. The iron export function of ferroportin is primarily regulated by the serum peptide hormone hepcidin, which is secreted from the liver when systemic iron levels are high. Hepcidin acts as a negative regulator of iron uptake by binding to ferroportin at the cell surface and inducing its internalization and degradation. Genetic defects in ferroportin, hepcidin, or the proteins involved with sensing systemic iron levels lead to iron overload diseases known as hereditary hemochromatosis. Using the tools of biophysics and cell biology, we sought to study ferroportin and its interaction with hepcidin in order to better understand this critical bottleneck in iron uptake and how genetic defects within ferroportin might lead to disease. We developed the first protocols for the overexpression, detergent-solubilization, and purification of recombinant ferroportin. We determined that detergent-solubilized ferroportin is a monomer capable of binding hepcidin in vitro. We characterized the expression and subcellular localization of ferroportin in mammalian tissue culture and determined that both the amino- and carboxy-termini of ferroportin are cytosolic. We developed cell-based assays for the hepcidin-induced internalization of ferroportin and used these to characterize the route of internalization from the plasma membrane through early endosomes to degradative lysosomal compartments. Using live-cell imaging techniques, we showed that this internalization depended on intact microtubules. We expanded this cell-biological study to include sixteen disease-related ferroportin mutants and reported that each mutant was expressed on the plasma membrane like wild-type ferroportin, but that only a subset of the mutants were capable of being internalized by hepcidin. These studies form a foundation for future biophysical and cell-biological studies of ferroportin function
Risk of tuberculosis after initiation of antiretroviral therapy among persons with HIV in Europe
No full textObjectives: Tuberculosis (TB) risk after initiation of antiretroviral treatment (ART) is not well described in a European setting, with an average TB incidence of 25/105 in the background population. Methods: We included all adult persons with HIV starting ART in the RESPOND cohort between 2012 and 2020. TB incidence rates (IR) were assessed for consecutive time intervals post-ART initiation. Risk factors for TB within 6 months from ART initiation were evaluated using Poisson regression models. Results: Among 8441 persons with HIV, who started ART, 66 developed TB during 34,239 person-years of follow-up (PYFU), corresponding to 1.87/10 0 0 PYFU (95% confidence interval [CI]: 1.47-2.37). TB IR was highest in the first 3 months after ART initiation (14.41/10 0 0 PY (95%CI 10.08-20.61]) and declined at 3-6, 6-12, and > 12 months post-ART initiation (5.89 [95%CI 3.35-10.37], 2.54 [95%CI 1.36-4.73] and 0.51 [95%CI 0.30-0.86]), respectively. Independent risk factors for TB within the first 6 months after ART initiation included follow-up in Northern or Eastern Europe region, African origin, baseline CD4 count < 200 cells/mm(3), HIV RNA > 100,000 copies/mL, injecting drug use , heterosexual transmission. Conclusions: TB IR was highest in the first 3 months post-ART initiation and was associated with baseline risk factors, highlighting the importance of thorough TB risk assessment at ART initiation. (c) 2024 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases
Jezične igre u nastavi gramatike hrvatskoga jezika
No full textSvrha učenja hrvatskoga jezika trebala bi se odnositi na razvoj izražajnih komunikacijskih sposobnosti. Temelj jezične nastave normativna je gramatika. Polaskom u školu kod djeteta nije razvijeno apstraktno i logičko mišljenje potrebno za razumijevanje lingvističkih suodnosa i zakonitosti. Nastava temeljena na učenju i poučavanju gramatike sastojala se od niza pravila koje je dijete učilo napamet bez mogućnosti njihove primjene u konkretnim govornim i pisanim situacijama. Učenike se osposobilo za jezičnu reprodukciju, a ne za uspješnu funkcionalnu komunikaciju. Komunikacijsko – funkcionalnim pristupom nastavi hrvatskoga jezika naglasak se premješta s lingvističke na komunikacijsku osposobljenost koja učeniku omogućuje svrhovitu primjenu jezičnoga znanja u svakodnevnom sporazumijevanju. Osnovni oblik nastavne djelatnosti zasnovane na komunikacijsko – funkcionalnom pristupu učenju je didaktička igra. Svaka komunikacijska situacija, kao i igra, osmišljene su u skladu s osnovnim didaktičkim načelima primjerenosti, zanimljivosti, životnosti, postupnosti i sustavnosti. Udžbenik postaje posrednik u prenošenju znanja i izvor novih znanja. Zakonom o udžbenicima za osnovnu i srednju školu utvrđen je Pravilnik u kojemu je određeno da nastavni predmeti u osnovnoj školi imaju udžbenik i pripadajuće dopunsko nastavno sredstvo. Udžbenici i radne bilježnice trebali bi imati različite zadatke za djecu različitih sposobnosti. U literaturi postoje različite podjele vrsta pitanja i zadataka. Za potrebe diplomskoga rada posebno je izdvojena podjela autorice Jadranke Nemeth – Jajić (2007). Analizirano je devet udžbeničkih kompleta s naglaskom na sadržaje jezika i jezičnoga izražavanja s ciljem ispitivanja zastupljenosti vrsta pitanja i zadataka preoblikovanih igrom. Istraživanje je provedeno prebrojavanjem broja pitanja i zadataka.The purpose of learning Croatian language should refes to development of communication skills. The foundation of linguistic education is grammar. When child is begining his formal education he does not have developed his abstract and logic oppinion that is necessary for understanding linguistic rules. Language teaching based on learning grammar consisted of rules that child should learn by heart without applying it in speaking and writting. The students were qualified for linguistic reproduction but not for successful communication. By using functional communication approach in language teaching emphasis is on using linguistic knowledge in everyday communication. The main form of functional communication approach is a didactic game. Every didactic game is conforming basic didactic rules. The text-book becomes agent in passing the knowledge. There is a Regulation that say that each subject in primary school should have text-book and additional teaching material. The text-books and workbooks should have different tasks for children with different abilities. There are different kinds of questions and tasks. In this master`s thesis I have specialy researched division of one author; Jadranka Nemeth-Jajić (2007). I have analyzed nine text-books and workbooks by counting questions and tasks
Evaluation of the expression of matrix metalloproteinase 9 and neutrophil gelatinase associated lipocalin in serum, urine and tumoral tissues of female dogs suffering from mammary gland tumors
No full textIn human medicine, it has been shown recently that the level of expression of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum, urine, and breast tissue were significantly increased in patients with breast cancer and correlated to several prognostic factors. In the first part, the author presents the MMP-9 and NGAL as a synthesis of current knowledge on their ability to serve as biomarkers of breast cancer in women. In the second, the author presents the production of canine MMP9 and NGAL recombinant proteins, the production of polyclonal antibodies, and their use in various techniques (ELISA, western blot, immunohistochemistry) to assess the level of expression of these proteins in the blood, urine and breast tissue of dogs suffering from mammary gland tumors and demonstrate a positive correlation between these proteins and the presence of a tumor disease of the mammary gland
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