7,924 research outputs found
Sobre as práticas e reflexões publicitárias de Fernando Pessoa
No full textNeste texto, a autora analisa alguns trabalhos publicitários de Fernando Pessoa, bem
como algumas das suas reflexões sobre esta actividade. É dada especial atenção ao slogan
“Primeiro estranha-se. Depois entranha-se”, analisado sobre a perspectiva das funções
e propriedades deste constituinte da mensagem publicitária. Por fim, recorda-se
um recente slogan que recupera o clássico de Fernando Pessoa.
On this article, the author analyses some advertising texts created by Fernando Pessoa and
some of his thoughts about this activity. The main attention goes to the famous slogan “Primeiro
estranha-se. Depois entranha-se”, analysed through the principles of the functions
and proprieties of this element of advertisements. Finally, the author presents a recent slogan, constructed through the slogan of the Portuguese writer
Implementación de una unidad de medida inercial mediante giroscopio y acelerómetro
No full text[ES]El objeto final de este trabajo de fin de grado es el estudio, diseño y
montaje de una unidad de medida inercial, o IMU. Los sensores utilizados en el
proyecto son un giroscopio, mide velocidades angulares, y un acelerómetro,
mide aceleraciones. Estos sensores están implementados en nuestra unidad de
medida inercial. Veremos cómo programar la unidad de medida inercial y
cómo se comunicará con el PIC18F4520.Tecles Morera, F. (2014). Implementación de una unidad de medida inercial mediante giroscopio y acelerómetro. https://riunet.upv.es/handle/10251/177020Archivo delegad
Análisis de colinesterasa en sangre entera de diferentes especies animales: estudios de validación analítica y clínica / Fernando Tecles Vicente ; directores Cándido Panizo, José J. Cerón Madrigal.
No full textTesis-Universidad de Murcia.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M.-2246.Consulte la tesis en: BCA. GENERAL. Fac. Veterinaria. Sala de estudio. Tesis-V 149
Fragments of the self: self-awareness, identity, and heteronymy in the works of Fernando Pessoa
No full textopenLa presente tesi esplora il tema dell'autocoscienza nell'opera di Fernando Pessoa, con particolare attenzione alla relazione tra identità ed eteronimia. Pessoa, attraverso la creazione dei suoi eteronimi, affronta questioni fondamentali legate alla frammentazione del sé e alla complessità dell'identità umana. L'analisi si concentra sulla natura poliedrica dell'autore, indagando come ogni eteronimo rappresenti un aspetto distinto della sua interiorità nel tentativo di comprendere e superare i limiti dell'individualità.This thesis explores the theme of self-awareness in the works of Fernando Pessoa, with a particular focus on the relashionship between identity and heteronymy. Through the creation of his heteronymys, Pessoa addresses fundamental questions related to the fragmentation of the self and the complexity of human identity. The analysis centers on the multifaceted nature of the author, investigating how each heteronym represents a distinct aspect of his inner world and an attempt to understand and transcend the limits of individuality
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Fernando Moral Iglesias writes to Dr. Hector P. Garcia about Bill Neeley, author of the book "Hispanic Patriots of the Texas Revolution." (Correspondence)
No full textFernando Moral Iglesias writes to Dr. Hector P. Garcia about Bill Neeley, author of the book "Hispanic Patriots of the Texas Revolution." Two newspaper articles about Bill Neeley are included
Estudios sobre alfa-amilasa en saliva como marcador de estrés en la especie humana y otras especies animales= Studies on salivary alpha-amylase as a maker of stress in human and different animal species
No full textOBJETIVOS
1. Evaluar el uso de la alfa amilasa salivar como marcador de estrés usando técnicas tradicionales (ensayos enzimáticos) en humana, usando como modelo experimental a estudiantes de veterinaria que tenían que realizar una presentación oral de un trabajo en lengua extranjera.
2. Evaluar el uso de la alfa amilasa salivar como marcador de estrés usando técnicas tradicionales (ensayos enzimáticos) en cerdos.
3. Implementar nuevos enfoques para la medición de la alfa-amilasa en muestras de saliva de varias especies (incluidos los seres humanos) mediante la cuantificación directa de la cantidad de la alfa-amilasa salival en lugar de mediante la medición de su actividad
4. Detectar e identificar nuevos biomarcadores de estrés en la saliva mediante técnicas de proteómica. Una técnica avanzada que permite la separación e identificación de múltiples proteínas.
METODOLOGÍA
Esta tesis doctoral es un compendio de los siguientes artículos publicados:
1) Assessment of Stress Associated with an Oral Public Speech in Veterinary Students by Salivary Biomarkers. Tecles F, Fuentes-Rubio M, Tvarijonaviciute A, Martínez-Subiela S, Fatjó J, Cerón JJ. (2014) J Vet Med Educ. Jan 21:1-7. [Epub ahead of print].
2) Validation of an automated method for salivary alpha-amylase measurements in pigs (Sus scrofa domesticus) and its application as a stress biomarker. Fuentes M, Tecles F, Gutiérrez A, Otal J, Martínez-Subiela S, Cerón JJ. (2011) J Vet Diagn Invest. Mar;23(2):282-7.
3) Porcine salivary analysis by two-dimensional gel electrophoresis in three acute stress models: a pilot study. Fuentes-Rubio M, Cerón J, de Torre C, Escribano D, Gutiérrez AM, Tecles F. (2014) Can J Vet Res. Apr;78(2):127-32.
En el primer artículo, se midió el cortisol salival y la actividad alfa-amilasa para evaluar el estrés asociado con una presentación oral pública de los estudiantes de Patología Clínica Veterinaria. Se recogieron muestras de saliva antes y directamente después de la presentación oral de 5 minutos y a los 20 y 35 minutos después del comienzo de la misma. El cortisol alcanzó su punto máximo 20 minutos después del comienzo de la presentación, mientras que la alfa-amilasa salival alcanzó su máximo justo al final del discurso. No existió relación entre estos cambios y el nivel de estrés que se indica por el estudiante en un cuestionario, ni con el sexo del estudiante, o la calidad de la presentación.
El objetivo del segundo estudio fue validar un método espectrofotométrico automatizado para la medición de alfa-amilasa salival en cerdos y evaluar su posible aplicación como biomarcador de estrés no invasivo . La validación analítica incluyó la precisión intra- e inter- ensayo, linealidad bajo dilución, y límite de detección. Además, para estudiar el posible uso de alfa- amilasa salival como marcador de estrés, 12 cerdos en crecimiento de 3-4 meses de edad fueron sometidos a estrés por inmovilización durante al menos 1 min, obteniéndose muestras de saliva en diferentes puntos de tiempo. Los resultados de la validación analítica indicaron que el método era preciso y capaz de medir la alfa-amilasa de una manera lineal. Los resultados obtenidos en la prueba de esfuerzo mostraron un aumento significativo en la actividad salival alfa amilasa.
El propósito del artículo 3 fue estudiar los cambios en el proteoma salival de cerdos sanos en situaciones de estrés para identificar cualquier posible nuevo biomarcador salival de estrés. Tres grupos de animales fueron sometidos a 3 modelos de estrés: restricción seguido de muestreo simulado de sangre de la vena cava; transporte breve por carretera; restricción de movimiento en una jaula digestibilidad. La saliva se obtuvo de cada animal antes, 15 y 30 min después de la inducción de estrés. Las muestras de los animales que mostraron el mayor incremento en la concentración de cortisol salivar se agruparon y se realizó una electroforesis en geles bidimensionales. Se tiñeron con Coomassie Brilliant Blue R-250 para la detección de spots y seleccionarlos para su identificación mediante espectrometría de masas. Los análisis estadísticos demostraron que 2 proteínas tenían diferencias significativas en la expresión antes y después de la inducción de estrés y fueron identificadas como fragmentos de albúmina y proteína de unión odorizante.
CONCLUSIONES
1. La actividad enzimática de la alfa amilasa salivar puede ser usada como biomarcador de estrés en estudiantes de Veterinaria tras someter a los individuos a una presentación pública oral.
2. La actividad enzimática de la alfa amilasa salivar puede ser usada como biomarcador de estrés en cerdos tras someter a los individuos a un protocolo de restricción de movimientos.
3. La concentración de alfa amilasa en saliva de humanos, cerdos, caballos y ovejas puede ser cuantificada mediante un ensayo de inmunofluorometría a tiempo resuelto. Este ensayo tiene una variación inter-individual baja y es más sensible que los ensayos enzimáticos para detectar estrés.
4. El proteoma salivar de los cerdos ha revelado diferencias entre animales en condiciones basales y bajo condiciones de estrés. Esto se reveló como una herramienta útil para buscar posibles nuevos biomarcadores de estrés in estos animales tales como la proteína de unión odorizante.
OBJECTIVES
1. To evaluate the use of salivary alpha-amylase as a marker of stress using traditional techniques (enzymatic assays) in human using a model in which veterinary students had to make an oral presentation in a foreing language.
2. To evaluate the use of salivary alpha-amylase as a marker of stress using traditional techniques (enzymatic assays) in pigs.
3. To implement new approaches for measuring alpha-amylase in saliva samples of several species (including human beings) by direct quantification of the amount of the salivary alpha-amylase instead of by measuring its activity (Article 3, Article 4, Article 5, Article 6).
4. To detect and identify new biomarkers of stress in saliva by proteomics. An advanced technique that allows separation and identification of multiple proteins.
METHODOLOGY
This PhD thesis is a compilation of the following published papers:
1) Assessment of Stress Associated with an Oral Public Speech in Veterinary Students by Salivary Biomarkers. Tecles F, Fuentes-Rubio M, Tvarijonaviciute A, Martínez-Subiela S, Fatjó J, Cerón JJ. (2014) J Vet Med Educ. Jan 21:1-7. [Epub ahead of print].
2) Validation of an automated method for salivary alpha-amylase measurements in pigs (Sus scrofa domesticus) and its application as a stress biomarker. Fuentes M, Tecles F, Gutiérrez A, Otal J, Martínez-Subiela S, Cerón JJ. (2011) J Vet Diagn Invest. Mar;23(2):282-7.
3) Porcine salivary analysis by two-dimensional gel electrophoresis in three acute stress models: a pilot study. Fuentes-Rubio M, Cerón J, de Torre C, Escribano D, Gutiérrez AM, Tecles F. (2014) Can J Vet Res. Apr;78(2):127-32.
In the first article, salivary cortisol and alpha-amylase activity were measured to assess stress associated with a public oral presentation in Veterinary Clinical Pathology students. Stimulated saliva samples were collected before and directly after a 5-minute oral presentation and at 20 and 35 minutes after the beginning of the presentation. Cortisol peaked 20 minutes after the beginning of the presentation, whereas salivary alpha-amylase (sAA) peaked at 5 minutes, just at the end of the speech. These changes were not related to the level of stress that was indicated by the student in a questionnaire, the student’s sex, or the quality of the presentation.
The aim of the second study was to validate an automated spectrophotometric method for salivary alpha-amylase measurement in pigs and evaluate its possible application as a noninvasive stress biomarker. The analytical validation included intra- and interassay precision, linearity under dilution, and limit of detection. In addition, to study the possible use of salivary alpha-amylase as a possible stress marker, 12 crossbred growing pigs of 3-4 months of age were subjected to restraint stress by a nasal snare for at least 1 min, and saliva samples were obtained at different time points. The results of analytical validation indicated that the method was precise and able to measure alpha-amylase in a linear manner. The results obtained in the stress test showed a significant increase in salivary alpha-amylase activity.
The purpose of the research of article 3 was to study changes in the salivary proteome of healthy pigs in stressful situations to identify any potential new salivary biomarker of stress. Three groups of animals were subjected to 3 stress models: snaring restraint followed by simulated sampling of vena cava blood; brief transport by road; and restriction of movement in a digestibility cage. Saliva was obtained from each animal before and 15 and 30 min after the induction of stress. The samples from the animals that showed the greatest increase in salivary cortisol concentration were pooled and run on 2-dimensional gels. Coomassie Brilliant Blue R-250 was used for spot detection and mass spectrometry for spot identification. Statistical analyses showed that 2 proteins had significant differences in expression before and after the induction of stress. These proteins were identified as odorant-binding protein and fragments of albumin.
CONCLUSIONS
1. The enzymatic activity of salivary alpha-amylase can be used as biomarker of stress in Veterinary students after subjecting the individuals to a public oral presentation.
2. The enzymatic activity of salivary alpha-amylase can be used as biomarker of stress in pigs after subjecting the individuals to restraint of movement protocol.
3. Alpha-amylase concentration in saliva of humans, pigs, horses and sheep can be quantified by TR-IFMA This assay has a lower inter-individual variation and is more sensitive than enzymatic assays to detect stress.
4. Salivary proteome of pigs has revealed differences between animals in basal status and under stress conditions. It was shown as a useful tool to search possible new salivary biomarkers of stress in these animals such as odorant-binding protein
Fernando Henrique Cardoso e o pensamento político brasileiro
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Sociologia Política, Florianópolis, 2015.A construção do legado de Fernando Henrique Cardoso tem transcorrido a partir do signo de rupturas e dicotomias estruturantes, características que se articulam em torno da ideia da transição da atividade intelectual para a atividade política, num processo que reforça traços marcantes da imaginação política brasileira. Partindo da crítica dessa visão, este trabalho investiga o que o processo de formação do legado do autor como levado a cabo até aqui tem a dizer sobre os modos pelos quais se produz e se reinventa o ?pensamento político brasileiro?, ou, em outras palavras, o que sua trajetória intelectual individual representa em relação à trajetória coletiva de pensamento designada por esta expressão. Duas teses interpretativas principais são desenvolvidas ao longo deste trabalho: a primeira, de que o fio de continuidade e de unidade entre os diversos momentos da trajetória intelectual de Cardoso, negado pelas abordagens que até o presente vão dando conta da sistematização de seu legado, pode ser reconstruído e recuperado se a ênfase da análise recair na especificidade política de seus escritos, reconstrução e recuperação que, obviamente, não se furtam a considerar possíveis inconsistências, incoerências e mudanças nas crenças ao longo de sua trajetória; e a segunda, de que tal pensamento político, sem desconsiderar outras fontes genericamente rotuladas como ?universais?, estrutura-se a partir de um diálogo seletivo, sistemático e contínuo com algumas das principais matrizes de interpretação do político no país, diálogo este que, de um lado, torna possível evidenciar a existência de uma cosmovisão a atuar como ?fio condutor? para o conjunto de seu pensamento (conferindo a ele um sentido de coerência) e, de outro, entender o impacto ressignificador desse pensamento sobre tais matrizes. O trabalho está organizado em três partes: na primeira, produz-se uma reflexão teórico-metodológica do campo do pensamento político brasileiro; na segunda, discute-se a especificidade da reflexão política do autor a partir de dois momentos distintos (o do estabelecimento e o da consolidação das suas crenças políticas); e na terceira, num exercício de tentar aproximar as duas primeiras, discute-se a especificidade da inscrição do pensamento político do autor no campo do pensamento político brasileiro a partir de duas variáveis: as filiações e as recepções.Abstract : The construction of Fernando Henrique Cardoso?s legacy has been developed based on the mark of structuring ruptures and dichotomies, characteristics that are both articulated around the idea of the transition from the intellectual to the political activity, in a process that reinforces strong features of the Brazilian political imagination. Anchored in a criticism of this view, this study investigates what the process of formation of the author?s legacy developed until the present moment reveal about the forms through which the ?Brazilian political thought? is produced and reinvented, or, in other words, what his personal intellectual trajectory represents in relation to the collective thought trajectory designated by this expression. Two main interpretative theses are tested throughout this work: the first one is that the continuity and the unity between the several moments of Cardoso?s intellectual trajectory (denied by the approaches that, until the present moment, have been systematizing his legacy) can be reconstructed and recuperated if the emphasis of the analysis lies in the political specificity of his writings - assuming that both the reconstructing and the recuperation, obviously, do not fail to consider possible inconsistencies, incoherences and changes in the beliefs of the author throughout his trajectory; and second , that such political thought , without disregarding other sources generally labeled as ?universal? , is structured from a selective, systematic and continuous dialogue with some of the main forms of interpretation of the policy in the country, this dialogue , on the one hand , makes it possible to show the existence of a worldview to act as ?common thread? for all of his thought (giving him a sense of coherence), and on the other, to understand the impact of this ressignificador thinking about such matrices. The work is organized in three parts: in the first, the field of the Brazilian political thought is theoretical and methodologically questioned; in the second part, the specificity of the author?s political reflection is discussed based on two different moments (the establishment and the consolidation of his political beliefs); and, in the third part, in an attempt of approximating the two previous parts, the specificity of the insertion of the author?s political thought in the Brazilian political thought field is discussed, which is based on two variables: the filiations and the receptions
Relaciones de Fernando Ortiz con los antropólogos españoles
No full textCon permiso de la revista para este artículo[EN] An approach to Fernando Ostiz's creative work-concerning not only the outcome of his scientific work, but also the way in which he developed it according to the social and cultural context he lived in-is proposed. Consequently, the contrast established by the author between the Cuban savant's image and the Spanish anthropologist Julio Caro Baroja is quite interesting. Other subjects regarding the links between Ortiz and his contemporary Spanish intellectuals are likewise considered.[ES] Aproximación a la labor creativa de Fernando Ortiz que atiende no sólo a los resultados de su obra científica, sino también a la forma en que desarrolló ésta en función del contexto sociocultural en que le tocó vivir. En ese sentido, resulta de interés la comparación que establece la autora entre la figura del sabio cubano y el antropólogo español Julio Caro Baroja, así como otros elementos en torno a los vínculos entre Ortiz y los intelectuales españoles de la época.Peer reviewe
Fernando Palazzi
No full textThe headword explains the biography and the contribution of the author Fernando Palazzi to the children's literatur
La senda iluminada (1949), de Fernando Vizcaíno Casas
No full textFernando Vizcaíno Casas is an author with multiple facets, although he was only recognised by his successfull leading articles during the Spanish Transition. Drama was one of less known biographical attitudes. It as never claimed by himself, despite of being a proud author. Anyway, he won prizes, controversial premieres and the satisfaction that La senda iluminada (1949) allowed him to participate in TEU.Fernando Vizcaíno Casas es un autor con múltiples facetas, aunque solo es recordado por sus éxitos editoriales durante la Transición. El teatro fue una de sus constantes biográficas menos conocidas. Nunca la reivindicó un autor tan satisfecho consigo mismo, pero tuvo premios, estrenos polémicos y la satisfacción de que La senda iluminada (1949) le permitiera participar en el TEU
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