287 research outputs found
What are the lessons that can be learned from Turkey's transnational operational police cooperation experiences?
No full textAlthough transnational organized crime and terrorism are not new, they are becoming increasingly intersected. Their combined activities threaten today‘s globalized world, making police collaboration across national borders more crucial than ever. As a consequence, bilateral and multilateral collaborations among national law enforcement agencies are increasing, and multinational police organizations are expanding their operations in order to cope more effectively with transnational crimes and terror. This dissertation explores the parameters of operational police cooperation across borders, by examining Turkey‘s policing relationships with other states as well as with international police cooperation organizations. Understanding how trans-border police cooperation works in practice, including what enhances or hinders such cooperation at the grassroots level, is the main objective of this study. The research methodology involved interviews with police professionals who had working experiences in these areas.Ph.D.Includes bibliographical referencesIncludes vitaby Huseyin Or
Does industry sponsorship bias research findings?
No full textA new study reveals that the findings obtained from industry sponsored studies for widely prescribed cholesterol drugs are similar in magnitude as those in non-industry sources. Huseyin Naci, LSE Health research fellow and co-author of the study, explains further
Vice-Chancellor's Community Engagement Award
No full textTeam award
Winner: SHINE: An innovative space project for university and high school students
Recipients: Huseyin Sumer, Eddie Brelsford, Elliot Henkel, Alan Duffy, Virginia Kilborn, Rebecca Allen
A POETIC NASIHAT-NAME WRITTEN FOR MURAD IV: MIFTAH-I CENNET OF QADIRI HUSEYIN
No full textMany nasihat-names have been written with the aim of improving the individual and society in Turkish Islamic Literature field. These works can be written for the general community, they are also written for a spesific part of the society such as women, children, brethren, state administrators. The work named Miftah-i Cennet of Qadiri Huseyin was written for Sultan IV. Murad, one of the 17th century Ottoman sultans. The aim of the work is to guide the sultan who ascended the throne at the age of just a child. Sultan is the collocutor of the advices given to lead him to the happiness of the world and the hereafter. In this study, Miftah-i Cennet that was written for Sultan IV. Murad and has the feature of a poetic nasihat-name will be introduced. In the first part of the study, which consists of two parts, the life and works of Qadiri Huseyin, the author of the work, which does not have any information about his life in the biographical sources, will be mentioned. In addition, the opinions that the person known as Muhyiddin-i Qadiri may be the same person as Qadiri Huseyin will be presented. In the second part, the copies of Miftah-i Cennet which is the subject of our study will be introduced and general information about the content of the work will be given
Political, social and historical issues in the novels of Huseyin Nihal Atsız
No full text13.09.2021 tarihine kadar kullanımı yazar tarafından kısıtlanmıştır.YÖK Tez No: 559045Hüseyin Nihal Atsız yaşantısı, fikirleri ve eserleriyle yaşadığı döneme damga vurmuş bir Türk aydınıdır. Fikirleriyle Türkçü nesillerin doğması ve yetişmesinde önemli etkisi olan Hüseyin Nihal Atsız'ın tarih alanında yapmış olduğu çalışmalar, bugün birçok araştırmacı tarafından kaynak olarak kullanılır. Edebî sahada vermiş olduğu eserlerle özgünlüğünü ortaya koyan Atsız, Türk edebiyatının önemli simalarındandır. Çalışmamızın ilk bölümünde Hüseyin Nihal Atsız'ın hayatına değinildi. İkinci bölümde yazarın Bozkurtların Ölümü, Bozkurtlar Diriliyor, Deli Kurt, Ruh Adam, Dalkavuklar Gecesi ve Z Vitamini romanlarındaki olaylara kısaca yer verildi. Üçüncü, dördüncü ve beşinci bölümlerde ise yazarın romanlarında tespit ettiğimiz siyasî, sosyal ve tarihî meseleler ayrı başlıklar altında incelendi.Huseyin Nihal Atsiz is a Turkish intellectual who made a mark in the period he lived, with his life, ideas and works. Huseyin, who has an important influence on the birth and development of Turkish generations with his ideas, is used by many researchers as a source for his studies in the field of history. Atsiz is a prominent figure of Turkish literature. In the first part of our study, the life of Huseyin Nihal Atsiz was mentioned. In the second chapter, the author's story of "Bozkurtların Ölümü, Bozkurtlar Diriliyor, Deli Kurt, Ruh Adam, Dalkavuklar Gecesi and Z Vitamini" novels was given. In the third, fourth and fifth chapters, the social, political and historical issues identified in the novels of the author were analyzed under different headings
Transmembrane delivery of specific recombinant proteins for reprogramming of somatic cells and disease therapy
No full textThe anionic plasma membrane is generally refractory to passive extracellular-to-cytoplasmic transit of proteins. A number of highly regulated endocytotic processes specify which extracellular proteins gain access to the cytosol, and which are excluded. Whilst critical for normal biological function in the natural setting, this property of the cellular plasma membrane represents major impedance to delivery of therapeutic proteins to cells, particularly delivery of large and/or charged proteins. The need to circumvent this membrane impermeability for research, or treatment of disease, led to development of various polycationic peptides (collectively termed cell penetrating/transduction peptides; CPP’s) that are capable of transmembrane transfer without disruption to the lipid bilayer (reviewed Sawant & Torchilin, 2010, and references therein). These were commonly devised from viral surface proteins or viral-host protein-protein interactions shown to be important to infection. The vast majority of CPP’s described to date (eg. Tat peptide, Penetratin; reviewed Deshayes et al., 2005) are non-selective in nature; ie. transmembrane transduction is achieved in most/all cell types. Interaction of CPP’s with lipid raft components/cell surface glycoproteins (often ubiquitously expressed across cell types) mediates cytoplasmic translocation via macropinocytosis, clathrin-mediated endocytosis, and/or caveolae/ lipid raft-mediated endocytosis, often in a concentration-specific manner (Duchardt et al., 2007, and references therein). Since host cell glycoproteins are ubiquitously expressed, many CPPs are non-selective in nature; ie. translocation occurs in most/all cell types (e.g. Tat peptide, Penetratin peptide; reviewed Deshayes et al., 2005). In a landmark study, Takahashi et al., (2006) forced expression of four key transcription factors (Oct4, Sox2, Klf4 and cMyc) in somatic cells to reprogram them to pluripotent, colony-forming phenotype that resemble embryonic stem cells (ES cells) by various criteria. Although this presents an opportunity to derive patient-specific stem cells for human disease therapy, elucidation of the molecular events that characterize the adoption of the pluripotent phenotype is required before their clinical applicability could be realized. I utilized a non-selective CPP to deliver key recombinant proteins to somatic cells in vitro, aimed at deciphering the temporal and molecular events that characterize early somatic cell reprogramming. Specifically, I investigated the concentration and temporal requirements of cMyc in repression of lineage associated genes (,eg. Thy1 in fibroblasts), a requisite biological event that precedes adoption of the pluripotent phenotype (Heffernan et al., 2011, submitted; Chapter 3). I describe construction of recombinant protein expression vectors incorporating (i) an arginine-rich basic domain (49-RKKRRQRRR-57) of HIV trans-activating transcriptional activator (Tat) protein (for transduction across cellular membranes), and (ii) mouse cMyc protein (denoted pTATmcMyc). Purification of semi-soluble/particulate pTAT-mcMyc recombinant protein preceded experiments highlighting contributions of cMyc and other reprogramming factors in repressing Thy1 in fibroblasts, suggesting a ‘cMyc-mediated’ and ‘default (cMyc absent)’ mechanism of Thy1 repression (Chapter 3). In chapters 4 & 5, I propose a theoretical framework for the treatment of multiple sclerosis(MS), a disease characterized by neural demyelination in the central nervous system (CNS). Conceptually, in vivo administration of fusion protein incorporating nonselective CPPs (as outlined Chapter 3) may treat disease that manifests across numerous/all cell types. However the full therapeutic potential of CPP’s will be realized when cell selective CPP’s are devised for cell-specific delivery of therapeutic proteins in vivo.Chapter 4 outlines preliminary development of a glial cell-specific CPP (gCPP), modeled on arenaviral infection of glia, for targeted delivery of therapeutic peptides. A screen of putative gCPPs in vitro highlighted one gCPP (termed ‘TD2.2) that effectively translocated to human glial cells (immature and matured oligodendrocytes, and astrocytes), yet appeared largely incapable of translocating to a non-glial (human) cell line. This tentatively demonstrated glial cell-selectively of the TD2.2 peptide sequence. Time course, sectional confocal microscopy provided further visual evidence for transduction of TD2.2 to human oligodendrocytes in vitro (Chapter 4). Myelin Associated Glycoprotein (MAG) is an oligodendrocyte-derived, periaxonal protein that regulates neural-glial cell signaling, structural/spatial integrity of myelin and Nodes of Ranvier and maintains glialaxonal cell interactions (Yang et al., 1996; Dashiell et al., 2002; Nguyen et al., 2009). The proteolytic cleavage of the periaxonal (extracellular) component of MAG by matrix metalloproteases (MMPs) results in loss of physical and molecular interactions of neural and glial cells, thus contributing to the progressive demyelination and axonal loss characteristic of MS (Sato et al., 1984; Moller et al., 1987; Tang et al., 1997; Stebbins et al., 1997; Milward et al., 2008). The mobile, digested product of MAG is also thought to represent a circulatory auto-antigen, further exacerbating disease. Chapter 5 of this thesis outlines theoretical design and construction of a mutated MAG protein (MAGMUT) capable of evading MMP mediated proteolysis. Following construction of protein expression vectors for expression (in E.coli) of histidinetagged, wildtype MAG (MAGWT) and MAGMUT recombinant protein, technical difficulties were encountered with induction and/or protein purification. In addition, MMP7 digestion experiments with oligodendrocytes expressing retrovirally delivered transgenes comprising EGFP-MAGWT and EGFPMAGMUT fusion protein were also somewhat inconclusive. Thus, validation of the ability of the MAGMUT sequence to evade MMP7-mediated proteolysis in vitro could not be conclusively drawn. In addition to a final discussion of experimental results and a proposal of future research directions, Chapter 6 addresses philosophical concerns if iPS technology and alternative strategies for deriving therapeutic cells (ie. transdifferentiation). Strategies to maximize persistence of recombinant protein in circulation, and functionality of recombinant protein in the cytosol, that could be adopted for in vivo validation of recombinant proteins (Chapters 4 & 5) are also discussed. To conclude, the myriad of possibilities in recombinant protein design, and relative ease of purification for screening of putative peptides, highlight the therapeutic potential of this technology for treatment of human disease. However, the full potential of this technology for human disease therapy will only be realized with concurrent development of strategies for targeted cellular delivery
Assessing the stability of the new marine DGPS station of Hong Kong and its connection to the WGS84 datum
No full textAuthor name used in this publication: Huseyin Baki IzVersion of RecordPublishedPublisher permissio
A novel method for somatic cell nuclear transfer to mouse embryonic stem cells
No full text© Mary Ann LiebertNuclear reprogramming by somatic cell nuclear transfer (SCNT) provides a practical approach for generating autologous pluripotent cells from adult somatic cells. It has been shown that murine somatic cells can also be reprogrammed to a pluripotent-like state by fusion with embryonic stem (ES) cells. Typically, the first step in SCNT involves enucleation of the recipient cell. However, recent evidence suggests that enucleated diploid ES cells may lack reprogramming capabilities. Here we have developed methods whereby larger tetraploid ES cells are first generated by fusion of two mouse ES cell lines transfected with plasmids carrying different antibiotic-resistance cassettes, followed by double antibiotic selection. Tetraploid ES cells grown on tissue culture disks or wells can be efficiently enucleated (up to 99%) using a combination of cytochalasin B treatment and centrifugation, with cytoplasts generated from these cells larger than those obtained from normal diploid ES cells. Also, we show that the enucleation rate is dependent on centrifugation time and cell ploidy. Further, we demonstrate that normal diploid ES cells can be fused to tetraploid ES cells to form heterokaryons, and that selective differential centrifugation conditions can be applied where the tetraploid nucleus is removed while the diploid donor nucleus is retained. This technology opens new avenues for generating autologous, diploid pluripotent cells, and provides a dynamic model for studying nuclear reprogramming in ES cells.Danièle Pralong, Krzysztof Mrozik, Filomena Occhiodoro, Nishanthi Wijesundara, Huseyin Sumer, Antonius L. Van Boxtel, Alan Trounson and Paul J. Verm
Grayanotoxin-III Detection and Antioxidant Activity of Mad Honey
No full textSahin, Huseyin/0000-0002-6018-1494WOS: 000359160500006Mad honey is a complex mixture of numerous chemicals produced by honeybees from Rhododendron flowers. Consumption of mad honey leads to diarrhea, perspiration, dizziness, changes in consciousness, syncope, diplopia, as well as blurred vision, hypotension, and bradycardia due to the presence of grayanotoxins (GTXs). Therefore, it is important to detect the level grayanotoxins in mad honey. Besides its toxicity, mad honey also has antioxidant activity. This study was designed to determine the level of grayanotoxin-III toxin and antioxidant activity of ten different mad honey samples collected from the Black Sea region of Turkey. Liquid chromatography-tandem mass spectrometry was used for the quantitation of grayanotoxin-III. Antioxidant activity was evaluated using total phenolic contents, total ferric reducing antioxidant power, scavenging of 2,2-diphenyl-1-picrylhydrazyl and 3-(2-pyridyl)-5, 6-diphenyl-1,2,4-triazine-4',4''-disulfonic acid radicals. Quantities of grayanotoxin-III levels ranged from 68.754 to 0.701 mu g grayanotoxin-III/g honey. Mad honey MH7 from Artvin/Hopa had the highest grayanotoxin-III level. Although there were varying levels of grayanotoxin-III, mad honey samples were outstanding in terms of antioxidant activity. MH3 had the highest antioxidant potential. Although toxicity effect comprises, a metered dose of mad honey might also be explored as a potential source in clinical trials due to high bioactivity levels.TAGEMGida Tarim Ve Hayvancilik Bakanligi [AR-GE/15]; Tubitak BidebTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)The study was supported by TAGEM AR-GE/15, and author Huseyin Sahin was supported by a grant from the research fund of Tubitak Bideb for his Ph.D. research
Chromatin modification of reprogrammed cells
No full text$AUD 272,812.50Early Career FellowshipsPeter Doherty Biomedical EC
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