27 research outputs found

    Perancangan dan Pembuatan Alat Pendeteksi Air pada Tangki Bahan Bakar Minyak Menggunakan Sensor Konduktivitas Berbasis Android

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    Has designed a detector Water in fuel oil tanks using a conductivity sensor based on Android. This device consists of a conductivity sensor for detecting water through conductivity properties of water in the fuel tank, Microcontroller ATMega 8 as a controller, an LCD to display the volume of water that was detected in the tank, the pump used to drain the water in fuel oil tanks, if the detected water content higher than the levels of fuel controller will get input, the pump will be activated to drain the water. Each volume is depleted in the tank can be seen on the LCD and any data from the sensors is sent to Android Via Bluetooth HC-05 is used as a remote monitoring system that results on android monitoring panel in Android.63 HalamanSkripsi Sarjan

    Identifikasi Spesies Ikan Ekonomis Penting yang Didaratkan di PPI Kedonganan dengan DNA Barcoding

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    Identifikasi spesies sangat esensial bagi inventarisasi keanekaragaman spesies ikan ekonomis penting yang terdapat di perairan Indonesia. DNA barcoding merupakan salah satu metode molekuler yang digunakan untuk identifikasi spesies kriptik. Penelitian ini bertujuan untuk  mengidentifikasi spesies ikan ekonomis penting yang didaratkan di PPI Kedonganan menggunakan metode DNA barcoding dengan marka control region mitokondria (mtDNA). Sebanyak 6 (enam) sampel dikoleksi dengan metode purposive random sampling. Hasil identifikasi dengan DNA barcoding menunjukkan sampel yang dikoleksi sebagai spesies Trichiurus lepturus nanhaiensis; Lutjanus quinguelineatus; Scomberus japonicus; Cheilopogon doederleinii; Parupeneus barberinus; dan Leiognathus equula. Jarak genetik intraspesifik menunjukkan kisaran nilai  0.003 – 0.346. Hasil identifikasi DNA barcoding menunjukkan hasil yang berbeda dengan hasil identifikasi morfologi awal. Hal ini dapat disebabkan oleh terbatasnya data sekuen spesies di dalam basis data genetik. Oleh karena itu, penelitian ini diharapkan dapat menjadi tambahan informasi genetik pada keanekaragaman spesies ikan yang terdapat di wilayah Indonesia, yang tercatat pada basis data genetik secara umum

    Resurrection of Indian Ocean humbug damselfish, Dascyllus abudafur (Forsskål) from synonymy with its Pacific Ocean sibling, Dascyllus aruanus (L.)

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    International audiencePrevious phylogeographic studies of the humbug damselfish, a widespread Indo-West Pacific coral-reef fish, have revealed a split of two main mitochondrial lineages distributed on either side of the Indo-Pacific barrier. This has been interpreted as the result of vicariance. It has been hypothesized that reproductive barriersmight currently limit gene flow between humbug damselfish populations from the Indian Ocean and the Pacific Ocean. In this study, we review the published phylogeographic information to update the distribution of the two main mitochondrial lineages of humbug damselfish. The Indian lineage was distributed from the Red Sea to the eastern extremity of the Sunda Shelf while the Pacific lineage, which diverged from the former by 0.6% net nucleotide divergence and diagnostic substitutions at three nucleotide sites at the cytochrome-b locus, was distributed east and north of the Sunda Shelf. The two forms, which are also genetically distinct at nuclear loci, were also characterized by distinct pigmentation patterns. We argue that the two forms represent geminate species. Epithet aruanus Linnaeus is maintained for the Pacific-Ocean humbug damselfish while epithet abudafur (Forsskål) is here resurrected for the Indian-Ocean humbug damselfish. Future studies should focus on the population genetic structure of the transition zone between Dascyllus abudafur and D. aruanus

    Redefining Dispersal Boundaries of Siganus fuscescens In The Coral Triangle Area

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    The increasing demand of fish in the Coral Triangle Area has led to overexploitation of some species of fishes. One of the commercial fishes, which is also known to be the source of food and income for local communities, is the Mottled Spinefoot (Siganus fuscescens). Population studies on this species are important in order to manage sustainable stock populations. Genetic variation of the mitochondrial DNA was analyzed to examine the population structure of Siganus fuscescens in Indonesia, as part of the Coral Triangle Area. In total, 789 basepairs of control region mtDNA sequences were determined from 133 specimens collected from six localities, including Seribu Islands (n=27), Karimunjawa (n=19), Komodo (n=39), Selayar (n=20), Lembeh (n=19) and Luwuk (n=9). From the data, 27 variable sites and 24 haplotypes were detected, with most of the haplotypes unique to each location. Haplotype data show that one haplotype was shared among all populations, three haplotypes were shared between two populations (Komodo & Selayar; Lembeh & Seribu; Komodo & Karimunjawa), and 20 were unique to a single population. Haplotype diversity (h=0.444) and nucleotide diversity (π=0.00165) were low. The diversity result, i.e. the ΦST value (0.0658, P < 0.0001) revealed genetic structure in S. fuscescens populations in Indonesia. A non-dispersal strategy led to restricted gene flow and genetic structuring in S. fuscescens. However, both the neutrality test and the mismatch distribution indicated that S. fuscescens might have been in populations at demographic equilibrium, with restriction to the population expansion. Although indicating unexpected minor population structure pattern, the overall result still suggest the management of this species population as a single unit across Indonesia

    Variasi Geografik Kelimpahan Zooplankton di Perairan Terganggu, Kepulauan Seribu, Indonesia

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    Zooplankton play a significant role in ecosystem as secondary producer. Climate change will potentially affect the abundance and global composition of zooplankton. This research aimed to study the geographical variation of zooplankton abundance relative to anthropogenic pressure. The research was conducted in April 2014 in area with presumably distinct anthropogenic level by its relative distance to the mainland of Jakarta. Samples were collected at Pramuka Island, Karang Beras Island, Kotok Island, and Sepa Island where subsequently lying to the nearest until furthest distance from mainland. Zooplankton samples were collected by towing the plankton net for 10 minutes for each site for three replicates. Abundance was obtained from Pramuka Island was 266698,214 ind/L, Karang Beras Island was 597363,1 ind/L, Kotok Island was 526447,8 ind/L and Sepa Island was 438225,3 ind/L. Overall, the closer to mainland, the richer nutrient and the more abundant zooplankton was conceived in the waters. Surprisingly, in Pramuka Island, the nearest island to mainland among other sites, had the lowest abundance of zooplankton although nutrient level was the richest among others.</jats:p

    Pengaruh Model Pembelajaran Talking Stick Terhadap Keaktifan Belajar Pendidikan Agama Kristen Siswa Kelas Vlll SMP Negeri 3 Lintongnihuta Tahun Pembelajaran 2023/2024.

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    The research aims to determine the positive and significant influence of the talking stick learning model on the active learning of PAK class VIII students at SMP Negeri 3 Lintongnihuta for the 2023/2024 academic year. The research hypothesis is that there is a positive and significant influence between the talking stick learning model on the active learning of PAK students in class VIII of SMP Negeri 3 Lintongnihuta for the 2023/2024 academic year. This research is a regression research using Descriptive Statistics techniques. The population of all class VIII students at SMP Negeri 3 Lintongnihuta for the 2023/2024 academic year who are Protestant Christians is 177 people. Random sampling (random sampling) is 30% of the total population, namely 55 people. The research instrument is a closed questionnaire. The results of the analysis requirements obtained the value rxy=0.554&gt;rtabel=0.226 and tcount=4.845&gt;ttable=2.000 showing that there is a relationship between the talking stick learning model and the learning activeness of PAK class VIII students at SMP Negeri 3 Lintongnihuta for the 2023/2024 academic year and obtained the regression equation. Hypothesis testing obtained Fcount=23.502&gt;Ftable=1.39 so H0 is rejected and Ha is accepted. The research concluded that there was a positive and significant influence of the talking stick learning model on the learning activeness of PAK class VIII students at SMP Negeri 3 Lintongnihuta for the 2023/2024 academic year, amounting to 30.69%

    The importance of standardization for biodiversity comparisons: A case study using autonomous reef monitoring structures (ARMS) and metabarcoding to measure cryptic diversity on Mo'orea coral reefs, French Polynesia.

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    The advancement of metabarcoding techniques, declining costs of high-throughput sequencing and development of systematic sampling devices, such as autonomous reef monitoring structures (ARMS), have provided the means to gather a vast amount of diversity data from cryptic marine communities. However, such increased capability could also lead to analytical challenges if the methods used to examine these communities across local and global scales are not standardized. Here we compare and assess the underlying biases of four ARMS field processing methods, preservation media, and current bioinformatic pipelines in evaluating diversity from cytochrome c oxidase I metabarcoding data. Illustrating the ability of ARMS-based metabarcoding to capture a wide spectrum of biodiversity, 3,372 OTUs and twenty-eight phyla, including 17 of 33 marine metazoan phyla, were detected from 3 ARMS (2.607 m2 area) collected on coral reefs in Mo'orea, French Polynesia. Significant differences were found between processing and preservation methods, demonstrating the need to standardize methods for biodiversity comparisons. We recommend the use of a standardized protocol (NOAA method) combined with DMSO preservation of tissues for sessile macroorganisms because it gave a more accurate representation of the underlying communities, is cost effective and removes chemical restrictions associated with sample transportation. We found that sequences identified at ≥ 97% similarity increased more than 7-fold (5.1% to 38.6%) using a geographically local barcode inventory, highlighting the importance of local species inventories. Phylogenetic approaches that assign higher taxonomic ranks accrued phylum identification errors (9.7%) due to sparse taxonomic coverage of the understudied cryptic coral reef community in public databases. However, a ≥ 85% sequence identity cut-off provided more accurate results (0.7% errors) and enabled phylum level identifications of 86.3% of the sequence reads. With over 1600 ARMS deployed, standardizing methods and improving databases are imperative to provide unprecedented global baseline assessments of understudied cryptic marine species in a rapidly changing world
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