5,616 research outputs found

    Group living homes for older people with dementia: Concept and effects

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    Eefsting, J.A. [Promotor]Pot, A.M. [Promotor]Depla, M.F.I.A. [Copromotor]Lange, J. de [Copromotor

    Cementbetonnen plaatbekledingen op oevers en dijken, bundeling van artikelen uit de vakpers 1990-1991

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    CUR; Proefproject: Open colloidaal beton a/s dijkbekleding; PT civiele techniek, april 1990. Burger, A.M., Eversdijk, P.J. en Hendriksma, A.M.; Open cementbeton toegepast a/s bekleding voor dijken; Zeewering Breskens, proefproject voor colloidaal beton; Land + Water, mei 1990. Burger, A.M., Eversdijk, P.J. en Hendriksma, A.M.; Colloidaal beton weerstaat zware storm en hoge golven; De praktijk van open cementbeton a/s Plaatbekleding; Land + Water, juni 1990. CUR; Cementbetonnen plaatbekledingen op dijken; Proefprojecten CUR; Civiele Techniek, No4, 1990. Vrieze, C.G. de; Betonnen dijken, groen a/s gras; Proeven met colloidaal beton voor begroeide rivierdijken; Land + Water No.6, juni 1991. Eversdijk, P.J. en Fase, A.G.; Breuksteen met colloidaal beton pakt rivierdijken goed in; Proefproject Opijnen in Julianakanaal; Land + Water No. 7/8, augustus 1991. Rijke, W.G. de en Burger, A.M.; Cementbetonnen plaatbekledingen op dijken en oevers; Praktische ontwerpmethode (1); Civiele Techniek, jaargang 46, No.3, 1991. Rijke, W.G. de en Burger, A.M.; Cementbetonnen plaatbekledingen op dijken en oevers theoretisch waterdicht; Praktische ontwerpmethode (2); Civiele Techniek, jaargang 46, No.4, 1991. CUR; oemonstratieproject open colloidaal beton Noordoostpolder; Civiele Techniek, No.3, 1991

    IoWoman, March/April 2004, Vol.34, no.2

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    Newsletter for the Iowa Commission on the Status of Wome

    IoWoman, March/April 2004, Vol. 34, no. 2

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    Newsletter for the Iowa Commission on the Status of Wome

    Gauge Fields and Strings

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    Based on his own work, the author synthesizes the most promising approaches and ideals in field theory today. He presents such subjects as statistical mechanics, quantum field theory and their interrelation, continuous global symmetry, non-Abelian gauge fields, instantons and the quantam theory of loops, and quantum strings and random surfaces. This book is aimed at postgraduate students studying field theory and statistical mechanics, and for research workers in continuous global theory

    The Sphinx Between the First and Second Pyramids. From a Photograph Taken by the Author about 6 a.m., After Sleeping at the Foot of the Sphinx.

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    Caption: "The Sphinx Between the First and Second Pyramids.From a photograph taken by the Author about 6 A.M., after sleeping at the foot of the Sphinx."The Great Sphinx with two pyramids behind. Black-and-white photograph

    Common Book Author Visit Highlights Spring Programming

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    Common Book author William Kamkwamba will visit campus on April 3 at 11 a.m. in Richardson Ballroom. All events are free and have cultural event status

    Author of \u27The Soloist\u27 to Speak

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    Steve Lopez, author of The Soloist, will speak at 9:30 a.m., Thursday, November 5, in Polsky Theatre as part of the JCCC scholar in residence program

    Filogeografia e dinâmica da diversidade genética de Acca sellowiana (O. Berg) Burret (Myrtaceae)

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    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2014.Acca sellowiana (O. Berg) Burret, conhecida comumente como goiabeira-serrana ou feijoa, é uma Myrtaceae que ocorre na região neotropical da América do Sul. A espécie possui potencial como frutífera, ainda pouco explorado no Brasil, mas bem difundido no exterior. O objetivo deste trabalho foi caracterizar a diversidade genética em nível de sequências de DNA de cloroplasto (cpDNA) e marcadores microssatélites nucleares (nSSRs) e, com base nesses dados, determinar relações filogeográficas, quantificar diversidade genética, avaliar estrutura genética espacial intrapopulacional, fluxo gênico e o sistema se cruzamento, afim de compreender o cenário evolutivo da espécie e contribuir para sua conservação e melhoramento. Para filogeografia foram sequenciados os espaçadores intergênicos plastidiais trnH-psbA e trnS-trnG em 32 populações, gerando 10 haplótipos. Oito haplótipos estão localizados ao norte da distribuição natural da espécie, em latitudes inferiores a 29o32?S e altitude superior a 500 m, sendo este o centro de maior diversidade da espécie. Foram encontrados indícios de expansão recente na distribuição meridional da espécie, mas isto deve ser comprovado com estudos adicionais, em razao do baixo polimorfismo das sequências analisadas. Visando elucidar esta possível expansão são indicadas análises com marcadores nSSRs. Foram encontrados dois padrões de distribuição da diversidade: i) na região com latitude inferior a 29o32?S a espécie é encontrada em altitude superior a 500 m em associação com a Mata de Araucária, a diversidade é maior e não apresenta expansão recente; ii) a partir de latitudes maiores que 29o65?S, a espécie ocorre abaixo de 400 m, a diversidade é menor e apresenta indícios de expansão recente. Para futuros estudos mais aprofundados em filogeografia foram desenvolvidos 10 novos locos nSSRs. Os novos marcadores apresentaram polimorfismo suficiente para análises genéticas, além de alguns deles serem transferíveis para outras espécies de Myrtaceae. Amostras de plantas de feijoa introduzidas e cultivadas fora da região de ocorrência natural foram caracterizadas através de nSSRs e mostraram uma diversidade restrita, evidenciando um gargalo genético decorrente da dispersão global da espécie a partir de poucos genótipos. Com base nessas análises foi possível verificar a similaridade dos acessos introduzidos com populações que ocorrem na região sul da distribuição natural da espécie. Oito nSSRs apresentaram equilíbrio genotípico e padrão de herança mendeliana, além de ligação genética não significativa, indicando o potencial deste conjunto de locos para a realização de análises como fluxo gênico e sistema de cruzamento. Esses mesmos marcadores foram utilizados para as análises intrapopulacionais, sendo confirmado que a espécie é preferecialmente alógama (tm = 0,895); porém, a área estudada indicou cruzamento entre parentes, que juntamente com o restrito fluxo gênico, levou à distribuição não aleatória dos genótipos e o agrupamento de indivíduos aparentados. Esses fatores contribuíram para a uma significativa estrutura genética espacial (até 54 m). Os resultados demonstram que os níveis atuais de fluxo gênico não são suficientes para evitar efeitos de deriva genética na população analisada. Em conclusão, este estudo gerou conhecimento sobre a história evolutiva de A. sellowiana, assim como sobre sua área de ocorrência, seu possível centro de origem e diversidade, e sua dinâmica da diversidade genética, nos níveis inter- e intra-populacional, trazendo novas informações com grande potencial de contribuir para novos estudos, programas de melhoramento e conservação da espécie.Abstract : Acca sellowiana (O. Berg) Burret, known as feijoa or pineapple-guava, is a Myrtaceae species from the neotropical region of South America. The species has potential for fruit production, which is underexploited in Brazil, but widespread in other countries. The objective of this study was to characterize the genetic diversity at the chloroplast DNA (cpDNA) sequence and nuclear microsatellite marker (nSSR) levels and, based on these data, to determine phylogeographic relations, estimate the genetic diversity, and evaluate intra-population spatial genetic structure, gene flow and mating system, in order to comprehend the species' evolutionary process and contribute to its conservation and breeding. For the phylogeography purposes the plastidial intergenic spacers trnH-psbA and trnS-trnG from plants of 32 populations were sequenced, generating 10 haplotypes. Eight haplotypes were found in the northern area of the species' natural distribution, at latitudes lower than 29o32?S and altitudes higher than 500 m, characterizing the region with the highest genetic diversity. Signatures of recent expansion in the southern portion of the species' distribution were detected, but this should be confirmed with aditional studies, due to the low polymorphism of the analyzed sequences. To address this possible expansion, further analyses with SSR markers are suggested. Two distinct patterns of diversity distribution were found: i) in lower latitudes (<29o32?S) the species is found at altitudes above 500 m, in association with the Araucaria Forest, the genetic diversity is higher and the populations do not show recent expansion; ii) in latitudes higher than 29o65?S, the species occurs at altitudes below 400 m, diversity is lower and there are signatures of recent expansion. Ten new nSSR loci were developed, and these new markers showed sufficient polymorphism for genetic analyses, with some of them being transferable to other Myrtaceae species. Samples of feijoa introduced and cultivated outside the region of natural occurrence were characterized through nSSR markers. The obtained data showed restrict genetic diversity, which indicates the existence of a genetic bottleneck in the global dispersion of the species, which probably started with few genotypes. In addition, it was possible to verify similarity between the introduced material and populations located at the southern portion of the species' natural ocurrence. To characterize intra-population spatial genetic diversity, gene flow and mating system, eight nSSRs were used. These nSSRs presented genetic equilibrium and mendelian inheritance, coupled to negligible genetic linkage, evidencing the potential of this set of loci forthe proposed studies. The intra-population analyses confirmed that the species has outcrossing mating system (tm = 0.895). However, crossings between relatives were found in the studied population, and that coupled to restrict gene flow led to a non-random distribution of genotypes and the clustering of related plants. These results reflect a significant spatial genetic structure (up to 54 m). In addition, the results indicate that current observed levels of gene flow are not sufficient to prevent genetic drift effects in the studied population. In conclusion, this study provided knowledge about the evolutionary history of A. sellowiana, including information about it's area of occurrence, its possible center of origin and diversity, the natural populations in which it occurs, and the dynamics of its genetic diversity, at the inter- and intra-population level, ultimately bringing new information with great potential to contribute to the species' study, breeding and conservation programs

    Allelic Diversity In Populations Of Solanum Lycocarpum A. St.-hil (solanaceae) In A Protected Area And A Disturbed Environment [diversidade Alélica Em Populações De Solanum Lycocarpum A. St.-hil (solanaceae) Em Unidade De Conservação E Em Ambiente Sob Influência Antrópica]

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    This study aimed to compare the genetic diversity of populations of Solanum lycocarpum A.St.-Hil between natural and human disturbed environments, with the assumption that protected areas have greater genetic diversity than disturbed areas. For this study, two populations were sampled in Goiás State, Brazil. One was located in a conservation unit, Serra de Caldas Novas State Park, in the Caldas Novas municipality. The other was located in a pasture area in the municipality of Morrinhos. The two populations are 41 km apart. We sampled 60 individuals from each population, which were genotyped with five microsatellite loci (SSR). The highest number of alleles was recorded in the population of the conservation unit, where we found 11 exclusive and five rare alleles. In the disturbed area, we recorded only three exclusive alleles and one rare allele. Although we did not observe significant inbreeding in these populations, genetic divergence between them was high (G ST (Hedrick)=0.147 =0.147) for a species with long distance seed dispersal. The results corroborate the hypothesis that the population in the less disturbed area harbors greater allelic diversity. They also confirm the effectiveness of using protected areas to preserve the genetic diversity of the species.254937940Buso, G.S.C., Brondani, R.V., Amaral, Z.P.S., Reis, A.M.M., Ferreira, M.E., (2000) Desenvolvimento De Primers SSR Para Análise Genética Depimentas E Pimentões (Capsicum Spp.) Utlizando Biblioteca Genômica Enriquecida, p. 15. , Brasília, Embrapa/Cenargen. (Boletim de pesquisaCouternay, Y., Conservation of the Maned Wolf: Fruitful relations in a changing environment (1994) Canid News, 2, pp. 41-43Crow, J.F., Aoki, K., Group selection for polygenic behavioral trait: Estimating the degree of population subdivision. Proceedings of the National Academy of Sciences of United States of América (1984) PNAS, 81 (19), pp. 607-677Degen, B., (2006) Genetic Data Analysis and Numerical Tests, , GDA_NT 2006, Grosshansdorf, Institute for Forest GeneticsFerreira, M.E., Grattapaglia, D., (1988) Introdução Ao Uso De Marcadores RAPD E RFLP Em Análise Genética, , 3 ed. Brasília, Embrapa - CenargenGoodman, S.J., RstCalc: A collection of computer programs for cal- culating estimates to genetic differentiation from microsatellite data and determining their significance (1997) Molecular Ecology, 6 (9), pp. 881-885Hedrick, P.W., A standardized genetic differentiation measure (2005) Evolu- Tion, 59 (8), pp. 1633-1638Lewis, P.O., Zaykin, D., (2000) Genetic Data Analysis: Computer Program For the Analysis of Allelic Data, , http://lewis.eeb.uconn.edu/lewishome/software.html, Version 1.0 (d15). Free program dis- tributed by the author over the internet from the GDA., Acesso em 23/01/2007Martins, K., Chaves, L.J., Buso, G.S.C., Kageyama, P.Y., Mating system and fine-scale spatial genetic structure of Solanum lycocarpum St. Hill. (Solanaceae) in the Brazilian Cerrado (2006) Conservation Genetics, 7 (6), pp. 957-969Martins, K., Chaves, L.J., Vencovski, R., Kageyama, P.Y., Genetic structure based on nuclear and chloroplast microsatellite loci of Sola- num lycocarpum A. St.-Hil (Solanaceae) in Central Brazil (2011) Genetics and Molecular Research, 10 (2), pp. 665-677Moura, T.M., Sebbenn, A.M., Chaves, L.J., Coelho, A.S.G., Oliveira, G.C.X., Kageyama, P.Y., Diversidade e estrutura genética espacial em populações fragmentadas de Solanum spp. do Cerrado, estimados por meio de locos microssatélites (2009) Scientia Forestalis, 37 (82), pp. 143-150Oliveira, F.A.T., Oliveira, L.C.A., Biologia floral de uma população de S. lycocarpum St. Hill. (Solanaceae) em Lavras MG (1988) Revista Brasileira De Botânica, 11 (1-2), pp. 23-32Vidal, M.C., Stacciarini-Seraphin, E., Camara, H.H.L.L., Crescimento de plântulas de Solanum lycocarpum St. Hil. (lobeira) em casa de vegetação (1999) Acta Botanica Brasilica, 13 (3), pp. 271-275Weir, B.S., Cockerham, C.C., Estimating F-statistics of the analysis of populations structure (1984) Evolution, 38 (6), pp. 1358-137
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