1,960 research outputs found
A novel load balanced energy conservation approach in WSN using biogeography based optimization
No full textClustering sensor nodes is an effective technique to reduce energy consumption of the sensor nodes and maximize the lifetime of Wireless sensor networks. Balancing load of the cluster head is an important factor in long run operation of WSNs. In this paper we propose a novel load balancing approach using biogeography based optimization (LB-BBO). LB-BBO uses two separate fitness functions to perform load balancing of equal and unequal load respectively. The proposed method is simulated using matlab and compared with existing methods. The proposed method shows better performance than all the previous works implemented for energy conservation in WSN
The Tale of a Choreographer, Her student, River and an endangered Heritage: Indu Mitha’s Qaseeda-i-Ilm of Jamal/ “An Ode to Wisdom and Beauty”
No full textChoreographing in Pakistan since the 50’s, the country’s senior most 90+ years young classical dance maestro Indu Mitha has made trailblazing contributions within the Kalakshtera Bharata Natyam using North Indian music, interesting and contemporary content, while also producing more tableau forms of dance.
In one of her recent solo pieces in the later style, titled “Qaseeda-i-Ilm of Jamal” or “An Ode to Wisdom and Beauty” Indu engages with symbolisms of a Hindu goddess of knowledge and Aesthetics_Saraswati and pays tribute to a forgotten dried up river of the same name. Indu Mitha allows the author, for whom and on whose body the dance is made, to bring in the forgotten river in her engagement with people’s histories of the land of present-day Pakistan and eventually facilitates her accessing of and embodying a pluralistic space of inter faith harmony which was occluded
Isolement de Salmonella nairobi d'un zébu Indu-Brasil en République du Zaïre
No full textUne entérobactérie ayant les caractéristiques d'une salmonelle est isolée en culture pure des prélèvements provenant d'un taurillon de race Indu-Brasil, mort quinze jours après le déclenchement d'une entérite aiguë. L'identification révèle qu'il s'agit de Salmonella nairobi. La seule salmonelle isolée jusque là dans cette région était S. dublin. Il s'agit du premier isolement de S. nairobi au Zaïre et du second isolement en Afrique à partir de l'espèce bovine</jats:p
Detrital zircon geochronology of quartzites from the southern Madurai Block, India: implications for Gondwana reconstruction
No full textAbstract not availableShan-Shan Li, M. Santosh, G. Indu, E. Shaji, T. Tsunoga
Knowledge representation issues in control knowledge learning
No full textSeventeenth International Conference on Machine Learning. Stanford, CA, USA, 29 June-2 July, 2000Knowledge representation is a key issue for any machine learning task. There have already been many comparative studies about knowledge representation with respect to machine learning in classication tasks. However, apart from some work done on reinforcement learning techniques in relation to state representation, very few studies have concentrated on the eect of knowledge representation for machine learning applied to problem solving, and more specically, to planning. In this paper, we present an experimental comparative study of the eect of changing the input representation of planning domain knowledge on control knowledge learning. We show results in two classical domains using three dierent machine learning systems, that have previously shown their eectiveness on learning planning control knowledge: a pure ebl mechanism, a combination of ebl and induction (hamlet), and a Genetic Programming based system (evock).Publicad
Isolement de Salmonella nairobi d\u27un zébu Indu-Brasil en République du Zaïre
No full textSee PDF file of the article.Vaya al archivo pdf del artículo.Une entérobactérie ayant les caractéristiques d\u27une salmonelle est isolée en culture pure des prélèvements provenant d\u27un taurillon de race Indu-Brasil, mort quinze jours après le déclenchement d\u27une entérite aiguë. L\u27identification révèle qu\u27il s\u27agit de Salmonella nairobi. La seule salmonelle isolée jusque là dans cette région était S. dublin. Il s\u27agit du premier isolement de S. nairobi au Zaïre et du second isolement en Afrique à partir de l\u27espèce bovin
Conformational Analysis And Design Of Disulfides In Antiparallel β-Sheets And Helices
No full textDisulfides are the primary covalent interactions within a protein molecule that connect residues which are sequentially distant. Naturally occurring disulfides enhance the stability of the protein by destabilization of the unfolded state. Previous attempts to introduce disulfide bridges as a means to enhance protein stability have met with mixed results. Tools have been developed to predict potential sites for disulfide introduction. However, it must be noted that engineering disulfides is not a trivial task. The effect of the engineered disulfide on protein stability is difficult to predict. There have been few systematic studies carried out to study disulfides in the context of secondary structures. The work in this thesis is aimed at studying disulfides in two kinds of secondary structures- antiparallel β-sheets and helices. In particular, the focus in this thesis is on cross-strand disulfides in antiparallel β-sheets and intrahelical disulfides. The analysis of naturally occurring disulfides in these structural elements coupled with protein engineering studies in model proteins were used to understand the effects of introducing disulfides in helices and antiparallel β-sheets.
Synopsis
This thesis also includes studies carried out on molten globules of four periplasmic binding proteins of E.coli- Maltose binding protein (MBP), Leucine, isoleucine, valine binding protein (LIVBP), Leucine binding protein (LBP) and Ribose binding protein (RBP). Work carried out in the lab previously had shown that these molten globules can bind the ligands that the proteins do in their corresponding native states. The analysis of the thermodynamic data obtained for these molten globules by differential scanning calorimetry (DSC) studies and isothermal titration calorimetry (ITC) to characterize stability and ligand binding respectively are described in this thesis. To further study the structural features of molten globules by fluorescence resonance energy transfer (FRET), double cysteine mutants of MBP were constructed and characterized. The rationale behind the construction of these mutants and their characterization is reported.
Chapter 1 gives an introduction to disulfides in proteins. Previous attempts at cataloguing and characterizing naturally occurring disulfides are described. An overview of studies carried out to determine the effects of removal of naturally occurring disulfides in proteins and the effect of engineered disulfides in different proteins is given. The various tools developed to predict potential disulfide sites are described. Chapter 1 also briefly discusses various aspects of molten globules and FRET.
Chapters 2 and 3 involve studies with cross-strand disulfides occurring in antiparallel β-sheets. A detailed analysis on various stereochemical aspects of naturally occurring cross-strand disulfides is described in Chapter 2. The reasons for these disulfides to almost exclusively occur at non-hydrogen-bonded registered pairs have been explored with conformational analysis, modeling studies and energy calculations. In Chapter 3, the effect of engineering cross-strand disulfides in four model proteins- LBP, LIVBP, MBP and Top7 are described. The ease of formation of the introduced disulfides and their effects on protein stability are described. The proteins with engineered cross-strand disulfides at exposed positions were also examined for redox activity. Our studies have shown that in antiparallel strands, engineered disulfides at exposed NHB registered pairs provide a robust means of increasing protein stability.
In Chapters 4 and 5, studies about intrahelical disulfides are described. In Chapter 4, the various conformational aspects of intrahelical disulfides occurring naturally are studied. Analysis of structures of proteins in conjunction with modeling studies show that all naturally occurring intrahelical residues bridge cysteines occurring between the N-Cap and 3rd residue of helices. To further explore conformational requirements for intrahelical disulfides, Cys pairs were introduced at N-terminal and interior of helices in a E.coli thioredoxin mutant lacking its active site disulfide. The ease of formation of the engineered disulfides, and their effects on protein stability were studied. The redox activity of the engineered disulfides was also examined. The studies demonstrated that intrahelical disulfides can only occur at the N-terminus of an α-helix and that the N-terminal CYS residue must adopt a non-helical backbone conformation. Although none of the engineered intrahelical disulfides increased the stability of the protein, they conferred mild redox activity. In Chapter 5, the ability of an engineered CXXC motif to bind Zn(II) is also explored. The effect of Zn(II) on the stability of the reduced and oxidized states of an engineered protein with a N-terminal intrahelical CXXC was ascertained. I have also shown that iminodiacetate (IDA) and nitrilotriacetate (NTA) resins charged with zinc can bind the protein CGPC 95-98 in reduced state. These Synopsis preliminary experiments on metal binding show that this property of CXXC motif could be exploited to develop a protein purification method.
In Chapter 6, thermodynamic characterization of molten globules of four periplasmic binding proteins (LBP, LIVBP, MBP and RBP) is described. Studies had been previously carried out in the lab to characterize the stability and ligand binding of these molten globules. All four molten globules were found to bind their corresponding ligands without conversion to the native state. In Chapter 6, the estimation of ΔCp of unfolding and ligand binding from the DSC and ITC data is described.
The binding of molten globules to their ligands and the ability to undego cooperative thermal unfolding indicated the presence of native protein-like tertiary contacts. To study the molten globule structure, we decided to construct double cysteine mutants of MBP for FRET studies. We decided to employ a strategy for differential labeling of the two cysteines with two different fluorophores based on the conformational differences between MBP in the ligand bound and free forms. Seven double cysteine mutants of MBP were made. The rationale behind the construction of these mutants and their preliminary characterization is described in the appendix to Chapter 6. The optimization of the differential labeling procedure of the MBP double mutants needs to be fine-tuned before further studies through FRET.
The work described in this thesis has resulted in the following publications:
1.Prajapati RS, Indu S, Varadarajan R. Identification and thermodynamic characterization of molten globule states of periplasmic binding proteins. Biochemistry. 2007 (46):10339-52.
1 Indu S, Kumar ST, Thakurela S, Gupta M, Bhaskara RM, Ramakrishnan C, Varadarajan R. Disulfide conformation and design at helix N-termini. Proteins.2010 (78):1228-42.
2 Indu S, Kochat V, Thakurela S, Ramakrishnan C, Varadarajan R. Conformational analysis and design of cross-strand disulfides in antiparallel β-sheets. (Manuscript submitted
Desafios na implementação do modelo MPS-BR: a percepção de um gestor da área de qualidade
No full textEste Trabalho de Conclusão de Curso foi julgado aprovado para a obtenção do Título de “Bacharel em Tecnologias da Informação e Comunicaçao”, e aprovado em sua forma final pelo Curso de Graduação em Tecnologias da Informação e Comunicação.O software é um produto muito importante na era da informação e comunicação. A oferta e o sucesso deste produto no mercado está diretamente relacionado à sua qualidade. Neste sentido, empresas buscam por meio da certificação uma forma de demonstrar sua qualidade. A avaliação do processo de desenvolvimento certifica que o software está de acordo com um conjunto de critérios básicos essenciais. No Brasil e no mundo a maior parte das empresas do setor de software são de pequeno e médio porte. No país existe o Programa MPS-B R (Melhoria de Processos do Software Brasileiro) com modelos de referência voltado para a realidade das industrias de software nacionais. Neste contexto, o objetivo deste trabalho é identificar os principais desafios enfrentados pelas empresas de pequeno e médio porte que buscam a certificação MPS-BR. Um estudo de pesquisa qualitativa e de caráter descritivo foi realizado por meio de uma entrevista para verificar a percepção de um gestor da área de qualidade. Os dados obtidos foram analisados a partir de outros estudos sobre o tema. Entre os principais resultados destacam-se: melhorias no produto final obtidas por meio da certificação, a resistência das equipes de trabalho ao processo de normatização, a relação custo-benefício da certificação para pequenas e médias empresas
Transmission and reciprocal transmission topological indices and co-indices of Indu-Bala products of graphs
No full textThe transmission of a vertex u in a connected graph G is denoted by s(?) and defined by [Formula presented]; that is, the sum of the distances between u and all other vertices of a graph G. The reciprocal transmission of a vertex u in a connected graph G is denoted by rs(u) and defined by [Formula presented]; that is, the sum of the reciprocal of distances between u and all other vertices of a graph G. In this paper we obtain explicit formulae for various transmission and reciprocal transmission based topological indices and co-indices of Indu-Bala product of graphs
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