123 research outputs found
Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo
No full textCitation: Girinathan, B. P., Braun, S., Sirigireddy, A. R., Lopez, J. E., & Govind, R. (2016). Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. Plos One, 11(7), 18. doi:10.1371/journal.pone.0160107Clostridium difficile is the principal cause of antibiotic-associated diarrhea. Major metabolic requirements for colonization and expansion of C. difficile after microbiota disturbance have not been fully determined. In this study, we show that glutamate utilization is important for C. difficile to establish itself in the animal gut. When the gluD gene, which codes for glutamate dehydrogenase (GDH), was disrupted, the mutant C. difficile was unable to colonize and cause disease in a hamster model. Further, from the complementation experiment it appears that extracellular GDH may be playing a role in promoting C. difficile colonization and disease progression. Quantification of free amino acids in the hamster gut during C. difficile infection showed that glutamate is among preferred amino acids utilized by C. difficile during its expansion. This study provides evidence of the importance of glutamate metabolism for C. difficile pathogenesis
Bacteriophage mediated toxin gene regulation in Clostridium difficle
No full textEmbargo status: Restricted to TTU community only. To view, login with your eRaider (top right). Others may request the author grant access exception by clicking on the PDF link to the left
Comparison of several author indices for gauging academic productivity
No full textBackground
Many author indices exist to gauge academic productivity. Several of these indices are calculated based upon an author's scholarly publication record, but the measurement methodology to calculate each index varies considerably, and the precise function being used, as well as the end result, is often complex and difficult to assess.
Method
Two straightforward methods to weigh author productivity from the publication and citation record were evaluated as possible means for providing a clearer assessment of scholarly activity. The author characteristic index (termed c-index) assigns author rank for each publication based upon author position. The characteristic prime (c') -index normalizes author rank from author position, so that the total weight per publication is unity. The top 10 scholars with keyword 'celiac disease' in the Google Scholar database were then assessed using these metrics. Rankings according to total number of publications, h-index, and c- and c'-indices were compared, then tabulated along with total papers included for assessment, and mean values per paper for author position, number of authors, citations, and year of publication.
Results
The order of the top ten authors with keyword 'celiac disease' varied substantially depending upon whether the h-index, c-index, or c'-index was used as a gauge. The characteristic indices assign credit to authors according to their position in an author list. The affiliated metrics provided a more complete picture of scholarly activity.
Conclusions
Academic achievement by scholars, based upon quantitative publication characteristics, has recently become of interest for evaluating job candidates, for determining work performance, and for bestowing awards and honors. The characteristic indices as described herein are readily calculated and interpreted, and may improve the assessment of scholarly activity
Scientometric Insights into Research Contributions of Govind Ballabh Pant University of Agriculture and Technology
No full textThis study evaluates the research productivity and impact of Govind Ballabh Pant University of Agriculture and Technology using Scopus data (2001–2021). A total of 4,897 publications receiving 53,059 citations were analyzed with scientometric indicators including AGR, RGR, DT, collaboration measures, authorship, citations, and keywords. Results show a gradual growth in research output, with peak productivity during 2017–2021 and Kumar, A. as the most prolific author (165 publications)
Colloidal engines for innovative tests of information thermodynamics
No full textRecent theoretical developments in information thermodynamics elucidated the link between the acquired information and the entropy production through measurement and feedback control by generalizing the fluctuation theorems and the second law of thermodynamics. We summarize here our recent experimental studies based on the colloidal system that have been conducted to test the theoretical findings of information thermodynamics. In particular, we present the design principles of error-free and noisy information engines consisting of a colloidal particle in an optical trap that is capable of performing nearly error-free measurement and ultrafast feedback control. Our perspectives on future experimental studies are also presented. ?? 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group
Secretion of Clostridium difficile toxins A and B requires the holin-like protein TcdE.
No full textThe pathogenesis of Clostridium difficile, the major cause of antibiotic-associated diarrhea, is mainly associated with the production and activities of two major toxins. In many bacteria, toxins are released into the extracellular environment via the general secretion pathways. C. difficile toxins A and B have no export signature and their secretion is not explainable by cell lysis, suggesting that they might be secreted by an unusual mechanism. The TcdE protein encoded within the C. difficile pathogenicity locus (PaLoc) has predicted structural features similar to those of bacteriophage holin proteins. During many types of phage infection, host lysis is driven by an endolysin that crosses the cytoplasmic membrane through a pore formed by holin oligomerization. We demonstrated that TcdE has a holin-like activity by functionally complementing a λ phage deprived of its holin. Similar to λ holin, TcdE expressed in Escherichia coli and C. difficile formed oligomers in the cytoplamic membrane. A C. difficile tcdE mutant strain grew at the same rate as the wild-type strain, but accumulated a dramatically reduced amount of toxin proteins in the medium. However, the complemented tcdE mutant released the toxins efficiently. There was no difference in the abundance of tcdA and tcdB transcripts or of several cytoplasmic proteins in the mutant and the wild-type strains. In addition, TcdE did not overtly affect membrane integrity of C. difficile in the presence of TcdA/TcdB. Thus, TcdE acts as a holin-like protein to facilitate the release of C. difficile toxins to the extracellular environment, but, unlike the phage holins, does not cause the non-specific release of cytosolic contents. TcdE appears to be the first example of a bacterial protein that releases toxins into the environment by a phage-like system
Review on Vaidyaka Paribhasha Pradipa- A Comprehensive Treatise of Indian Pharmaceuticals by Govind Sen
No full textVaidyaka Paribhasha Pradipa was written by Govind Sen, son of Krishna Vallabh Sen. Vidyotini Hindi Tika was written by Indradev Tripathi. The book Vaidyaka Paribhasha Pradipa consists of almost all references collected from various Samhitas regarding the fundamental principles and different Ayurvedic herbal pharmaceutical preparations and descriptions on Panchakarma. The whole content of the book is divided into 4 Khandas. Prathama khanda deals with Mana paribhasha, Dravya samgrahana vidhi, and shelf life of different Kalpana etc. Dwitiya khanda deals with Panchavidha kashaya kalpana and its Upakalpana and dose. Tritiya khanda deals with Sneha kalpana, Sandhana kalpana and Paribhasha of different Gana. Chaturtha khanda deals with Panchakarma procedures and Sneha murchana. The present book review mainly focuses to highlight the framework of Vaidyaka Paribhasha Pradipa, provides information about the author, details of 4 Khandas, a special contribution to the field of pharmaceutical science by the author. Thus, Vaidyaka Paribhasha Pradipa, the compilation book on Bhaishajya Kalpana is a very essential and mandatory book for those who aspire to gain basic, clear, and thorough knowledge in Ayurvedic pharmaceuticals. It is one of the indispensable reference books. The present book is designed to help the young practitioners who prepare medicines and graduates and post-graduate scholars get a clear idea of medicine preparation
Study of Numerical Solutions for the Deformations of A Bourdon Tube
No full textTitle: Study of Numerical Solutions for the Deformations of A Bourdon Tube, Author: Govind Prashad, Location: ThodeThe objective of this study is to compute numerically the deformations on an elliptical cross-sectional bourdon tube by solving the partial differential equations as presented
by Lee [Reference 12]. The partial differential equations and boundary conditions are reduced to a set of simultaneous linear equations by approximating partial derivatives to the corresponding difference quotients sing finite difference techniques. The next step involves the solution of this set of linear equations. The direct method of inverting the matrix was not possible to the memory limitations of the computer. Therefore, a block iteration technique was used, but it was found that convergence was not possible. The next method evolved was that of double iteration. for this method a convergence test was applied which indicated that convergence was possible, but the rate of convergence was very low. It is not practical to use this method, unless the convergence rate is improved. At the present no method is available to improve the convergence rate effectively. Therefore the study concludes with suggestions that either the convergence rate should be improved by evoking new methods or an entirely new formulation of the problem should be made.ThesisMaster of Engineering (ME
UniSQ Open Access Week 2025
No full textThis event will delve into the experiences of returning and new authors. The session will explore the the authors' journey through the book creation process, and the benefits, including the extension of their academic and professional reach.
Returning authors, Drs Anna Chruscik and Louisa Windus initially adapted an Open Stax textbook to create 'Fundamentals of Anatomy and Physiology'. They have embarked on a new project; a six volume set entitled 'Biosciences for the Health Professionals', the first of which has already been published.
New author Dr Chris Kossen was inspired to create his book 'Microlearning - Engaging Learning Experiences Made Easy' based on research and practice leading to a national teaching award. Since publication, Dr Kossen has attracted international collaboration, and research dissemination opportunities.
Dr Govind Krishnamoorthy has just finished a third volume dealing with Trauma Informed Practice, titled ‘Recovery and Resilience at School’ written with Dr Kay Ayre (University of Western Australia) and Dr Dayna Schimke University of Southern Queensland). This text provides interest as it converted an existing podcast series into a textbook, and provides diverse, authentic, and international practitioner perspectives.
The authors will share practical insights on open publishing and provide reflections on how this has enhanced their academic and research profiles
FACS analysis of <i>C. difficile</i> cells for membrane permeability through propidium iodide (PI) and SYTO staining.
No full text<p><b>A.</b> The viability standard samples containing the heat killed and actively growing <i>C. difficile</i> cells at 1/100, 50/50 and 100/1 ratio, respectively. <b>B.</b> The <i>tcdE</i> mutant and the parent JIR8094 cells collected from the overnight (16 h) cultures, were subjected to FACS analysis following propidium iodide (PI) and SYTO staining.</p
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