1,720,982 research outputs found
Analisis Molekuler Latar Belakang Genetik Galur-Galur Harapan Padi Ciherang Aromatik (BC5F6)
No full textPadi aromatik lokal Pandan Wangi dan Mentik Wangi telah dikembangkan
sebagai tetua donor dalam pembentukkan varietas unggul padi aromatik dengan
padi Ciherang sebagai tetua pemulih nonaromatik yang memiliki karakterisasi
unggul. Penelitian ini bertujuan menganalisis persentase genome recovery tetua
pemulih dari dua set populasi backcross (BC5F6) melalui seleksi background
genetik dengan bantuan marka molekuler. DNA padi tetua dan galur BC5F6
berhasil diisolasi dan diamplifikasi dengan primer polimorfik terpilih hasil survei
primer. Persentase pemulihan genom alel Ciherang pada galur BC5F6 populasi
Ciherang-Pandan Wangi mencapai 98.95% pada semua kedelapan individu,
kecuali individu nomor 4, 7, dan 8 yang masing-masing bernilai 86.92%, 96.99%,
dan 97.91%. Persentase pemulihan genom tiap sembilan individu galur BC5F6
populasi Ciherang-Mentik Wangi seragam sebesar 80%. Visualisasi data juga
dilakukan menggunakan perangkat lunak Graphical Genotypes (GGT) versi 2.0
Genetic Diversity of Japonica Rice (Oryza sativa L.) Based on Markers Corresponding to Starch Synthesizing Genes
Full text linkGenes related to starch synthesis and the metabolism contribute to a variety of physicochemical properties that determine the eating/cooking qualities of rice. Our previous study suggested that a set of molecular markers was able to estimate the eating quality of japonica rice. The present study reports the genetic diversity of 22 japonica rice varieties based on markers corresponding to starch synthesizing genes. The mean of the polymorphic information content (PIC: 0.135) value and the diversity index (0.171) indicated a low genetic diversity in these varieties. The phylogenetic tree clearly demonstrated three main clusters: 1) cluster I contained seven varieties with similar physicochemical properties; 2) cluster II only showed a Japanese variety, Koshihikari, and 3) cluster III included the most Korean varieties (14 varieties). This phylogenetic analysis did not completely represent the physicochemical properties differentiation of the japonica varieties, although it did reveal an initial clue to the close relationship between Korean rice and the Japanese and Chinese varieties. Notably, these markers were also able to identify a premium japonica rice. The molecular markers and information concerning the genetic relationship would be useful in improving the japonica rice along with its starch quality of in breeding program
Comparison of detergent and CTAB method for isolation of DNA from Salak ( Salacca zalacca (Gaert.) Voss. ‘Pondoh’)
Full text linkThis study conducted in Laboratory of Molecular Biology, Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian (BB- Biogen) Bogor. The aims of this study are to determine and comparing the quantity, quality and the efficiency of DNA isolation result using detergent method and CTAB method. The parameters observed in this study are the value of DNA concentration, purity, and visualization result using gel electrophoresis. The samples are the leaves of Salak ‘Pondoh’ (Salacca zalacca (Gaert.) Voss.). Detergent method is a method which was developed by Faculty of Biology UGM, it has simple method and relatively affordable cost. Meanwhile, CTAB method is one of the commonly used methods of DNA isolation protocol with relatively expensive cost. Detergent method used detergent in the cell wall separation and protein removal in the sample. The CTAB method used Cetyltrimethyl ammonium bromide (CTAB) for cell membrane separation in the sample. The research methods included DNA isolation with detergent and CTAB methods, PCR analysis and electrophoresis. Data analysis was done quantitatively using spectrophotometric method and qualitative used electrophoresis method. The result of the study showed that DNA isolation using CTAB method showed higher purity compared with detergent method with the purity values ranging from 1,3- 1,4 . Meanwhile, the concentration of DNA in the detergent method was higher than that of CTAB with the highest concentration of 1730 µg/ml. There is no difference between the quality of genomic DNA isolated by CTAB and detergent methods
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Keragaman Genetik Isolat Cendawan Pyricularia oryzae Menggunakan Primer Pot-2 (Rep-PCR)
No full text<p>Rice blast (Pyricularia<br />oryzae) is one of the most important diseases of rice. It can<br />be very destructive in the field, when the environmental<br />conditions are favourable. Information on genetic diversity of<br />this pathogen could assist plant breeders in determining<br />strategy for a successful control of the disease. This study<br />was conducted to analyze genetic diversity in P. oryzae<br />isolates by a pair of Pot-2 primers using the rep-PCR<br />technique. These primers were designed from a transposon<br />element of the entire blast fungus genomic DNA. DNA<br />samples were extracted from 212 isolates of P. oryzae<br />collected from two endemic areas of the disease in<br />Indonesia, i.e., Tamanbogo, Lampung, and Sukabumi, West<br />Java, as well as from some non-endemic areas in North<br />Sumatra and West Sumatra). Results of the study indicated<br />that the 212 isolates could clustered into 21 haplotypes. The<br />most dominant haplotypes as indicated by their highest<br />frequency of haplotypes were haplotype Pot 2-019 (54.46%)<br />followed by haplotype Pot 2-021 (14.73%) and haplotipe Pot<br />2-016 (6.25%). Regardless of origins of the P. oryzae isolates,<br />we found 6 haplotypes from Tamanbogo (out of 117<br />samples), 13 haplotypes from Sukabumi (out of 77 samples),<br />and 11 haplotypes from North Sumatra and West Sumatra<br />(out of 18 isolates). It seems that genetic diversity of the P.<br />oryzae isolates was not affected by the total number of<br />samples/isolates, but rather by place of the origin and rice<br />genotypes from which the isolates were collected.</p></jats:p
THE POTENTIAL USE OF SSR MARKERS TO SUPPORT THE MORPHOLOGICAL IDENTIFICATION OF INDONESIAN MUNGBEAN VARIETIES
Full text link<p>Mungbean varieties were mainly characterized based on morphological traits. Molecular genetic approach is expected to help the breeder in identification of mungbean varieties in more detail and to protect intellectual property right. This study aimed to identify Indonesian mungbean varieties based on DNA fingerprint profile using a marker set to support morphological characters. A total of 22 Indonesian mungbean accessions were characterized based on 21 morphological traits and 55 simple sequence repeats (SSRs) primers. Of the total 22 mungbean varieties used in the present study, 16 varieties were improved varieties and remaining six varieties were local varieties originated from Java, Nusa Tenggara and Sulawesi collected in GeneBank of ICABIOGRAD. The results showed that the 21 morphological characters were not sufficient to differentiate 22 mungbean varieties, while SSR analysis revealed that eight multi-alleles markers and high polymorphic information content (PIC) values have been successfully selected for varietal identification. The selected markers enabled to differentiate each mungbean variety according to their genetic marker with the lowest distance of 0.125, demonstrating the robustness of the selected marker set as a tool to identify a specific DNA fingerprint profile as a varietal identity (ID). The genetic identity of a variety was shown by digital barcoding which represented a series of alleles produced by corresponding markers. The DNA fingerprint profile of each variety would be beneficial as reference identities of a mungbean variety. <strong></strong></p></jats:p
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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