300 research outputs found
Art. 190. Baratto amministrativo - Partenariato pubblico privato
No full textIl baratto amministrativo ha trovato ingresso nella parte IV, titolo I del codice intitolato Partenariato pubblico privato. Si configura come uno speciale istituto di partenariato orizzontale o sociale. Il baratto amministrativo è considerato un nuovo sintagma che si è imposto nella pubblicistica ed evoca uno scambio avente ad oggetto, da una parte lo svolgimento di interventi di pubblica utilità a carico di taluni soggetti privati, dall’altra parte lo sconto tributario a favore di quegli stessi soggetti ad opera dell’ente pubblico competente
Alle origini del baratto: l'Odin in Sardegna 1974-1975
No full textAbstract – IT La complessa vicenda del “baratto”, ideata e attuata da Eugenio Barba e l’Odin Teatret a partire dal 1973-74, è una delle più affascinanti imprese appartenenti alla storia del Nuovo Teatro del Novecento. Il saggio ricostruisce il più oggettivamente possibile la permanenza dell’Odin Teatret in Sardegna, tra il 1974 e il 1975, durante la quale le tante comunità sarde risposero spontaneamente alla proposta dell’Odin, "barattando" il proprio patrimonio di canti, balli e pratiche tradizionali. Nato nella città di Cagliari, l’autore ha potuto spesso cogliere nelle testimonianze di intellettuali e artisti isolani l’eco di quella avventura; una traccia viva, quando non proprio un modello mitico, per più di una generazione di operatori culturali tuttora attivi in Sardegna. Da qui il desiderio di ricucire il filo della memoria, nell’idea di restituire quanto più possibile anche una sottile zona del rimosso, osservando con attenzione le date di questa vicenda e alcune testimonianze ad esse correlate. In particolare, secondo l’autore, la così tanto celebrata fase avvenuta a Carpignano Salentino da maggio a ottobre del 1974, a cui si attribuisce convenzionalmente, da un punto di vista storico, la nascita del baratto, in realtà ha teso a oscurare un precedente assai rilevante, legato all’incursione in terra sarda di Barba e compagni, tra Campidano e Barbagia, avvenuta nel mese di gennaio dello stesso anno. Abstract – EN The complex history of the 'baratto', which was conceived and performed by Eugenio Barba and the Odin Teatret from 1973-74, is one of the most challenging and fascinating projects in the history of XX century theatre. The purpose of the present essay is to retrace, as objectively as possible, Odin's permanence in Sardinia between 1974 and 1975, a period during which many local communities gave a positive feedback to Barba's project by 'bartering' traditional songs, dance and rituals. Born in Cagliari (Sardinia's main city) the author has caught the echo of that experience through the voices of intellectuals and local artists; an experience that has become a definitive benchmark, a legendary event for more than a generation of cultural operators still active in the island. Stitching up the thread of memory, the author restores, as far as possible, a bygone history through a meticulous reconstruction of dates and statements. Particularly, the essay affirms that the celebrated period of Carpignano Salentino (May-October 1974), to which the birth of the 'baratto' is generally attributed, has overshadowed this very significant first Sardinian journey in Campidano and Barbagia at the very beginning of 1974
Le ragioni per dire no al "baratto amministrativo"
No full textL'articolo motiva le ragioni per le quali il ricorso al baratto amministrativo non deve essere confuso con le esperienze di amministrazione condivisa, dove le amministrazioni costruiscono alleanze con i cittadini per la risoluzione di problemi di interessi generali. Inoltre, l'autore mette in rilievo tutta una serie di problemi che mettono in discussione anche la legittimità di tali scelte.The article explains the rationales preventing the confusion between the experiences of "administrative barter" policies and the so called "sharing administration", where local authorities and citizens pool together in order to resolve problems of general public interest. In addiction, the author points out a range of problems coming from the "administrative barter" policies in terms of legitimac
A psicologia psicanalítica do ego e a psicanálise freudiana: diferenças teóricas fundamentais
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-Graduação em Psicologia.Desenvolvemos neste trabalho de dissertação as teses fundamentais da escola norte-americana de psicanálise: a "Psicologia Psicanalítica do Ego", pautando-as com as teses fundamentais da psicanálise freudiana. São estabelecidas as diferenças teóricas que a "Psicologia Psicanalítica do Ego" guarda em a psicanálise freudiana, estabelecendo-se os pontos específicos em torno dos quais estas diferenças ocorrem
Identification of an iron-sulfur cluster that modulates the enzymatic activity in NarE, a Neisseria meningitidis ADP-ribosyltransferase.
No full textIn prokaryotes, mono-ADP-ribose transfer enzymes represent a family of exotoxins that display activity in a variety of bacterial pathogens responsible for causing disease in plants and animals, including those affecting mankind, such as diphtheria, cholera, and whooping cough. We report here that NarE, a putative ADP-ribosylating toxin previously identified from Neisseria meningitidis, which shares structural homologies with Escherichia coli heat labile enterotoxin and toxin from Vibrio cholerae, possesses an iron-sulfur center. The recombinant protein was expressed in E. coli, and when purified at high concentration, NarE is a distinctive golden brown in color. Evidence from UV-visible spectrophotometry and EPR spectroscopy revealed characteristics consistent of an iron-binding protein. The presence of iron was determined by colorimetric method and by an atomic absorption spectrophotometer. To identify the amino acids involved in binding iron, a combination of site-directed mutagenesis and UV-visible and enzymatic assays were performed. All four cysteine residues were individually replaced by serine. Substitution of Cys(67) and Cys(128) into serine caused a drastic reduction in the E(420)/E(280) ratio, suggesting that these two residues are essential for the formation of a stable coordination. This modification led to a consistent loss in ADP-ribosyltransferase activity, while decrease in NAD-glycohydrolase activity was less dramatic in these mutants, indicating that the correct assembly of the iron-binding site is essential for transferase but not hydrolase activity. This is the first observation suggesting that a member of the ADP-ribosyltransferase family contains an Fe-S cluster implicated in catalysis. This observation may unravel novel functions exerted by this class of enzyme
Combined approach of EPR and PCA analysis as a tool for clustering soils and leaves of olive groves and vineyards of different geographical origin
No full textThe Electron Paramagnetic Resonance (EPR) technique can be used to study agri-food matrices. This versatile technique provides specific insights into paramagnetic centres without requiring sample treating. In this paper, the EPR spectroscopy has been employed to investigate soils and leaves from vineyards and olive groves. A total of 33 soils and 31 leaves samples from olive groves, along with 53 soils and 19 leaves samples from vineyards, were collected from nearby areas in the south of Tuscany region (Italy). The EPR spectra of soils and leaves were analyzed both quantitatively and qualitatively on the basis of paramagnetic metal ions like manganese, iron and free radicals. A first classification was performed quantifying the different amounts of manganese signal in the samples. Nevertheless, the combined use of the EPR analysis with multivariate techniques like Principal Component Analysis (PCA), provides us a novel and fast tool for clustering samples on the basis of their geographical origin
Antioxidant activity of galloyl quinic derivatives isolated from P-lentiscus leaves
No full textThe antioxidant properties of galloyl quinic derivatives isolated from Pistacia lentiscus L. leaves have been investigated by means of Electron Paramagnetic Resonance spectroscopy (EPR) and UV-Vis spectrophotometry. Antioxidant properties have been also estimated using the biologically relevant LDL test. The scavenger activities of gallic acid, 5-O-galloyl, 3,5-O-digalloyl, 3,4,5-O-trigalloyl quinic acid derivatives, have been estimated against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide (O-2(-)) radical, and hydroxyl (OH) radical. On the whole, the scavenger activity raised as the number of galloyl groups on the quinic acid skeleton increased. The half-inhibition concentrations (IC50) of di- and tri-galloyl derivatives did not exceed 30 muM for all the tested free radicals. All the tested metabolites strongly reduced the oxidation of low-density lipoproteins (LDL), following a trend similar to that observed for the scavenger ability against OH radical
Association With Outcome of the Regurgitant-Volume Adjusted Right Ventricular Ejection Fraction in Secondary Tricuspid Regurgitation
No full textBackground: In patients with secondary tricuspid regurgitation (STR), right ventricular ejection fraction (RVEF) may not accurately reflect the actual RV systolic performance since a considerable amount of the RV stroke volume (SV) is regurgitated back into the right atrium. To overcome this limitation, we explored the association with the outcome of the effective RVEF (eRVEF), which accounts for the tricuspid regurgitant volume (RegVol). Methods: Five hundred thirteen patients with STR (mean age 75 ± 13 years, 39% atrial STR, 58% severe) underwent complete two-, three-dimensional, and Doppler echocardiography. The eRVEF was computed as RV forward SV/RV end-diastolic volume, where forward SV was obtained by subtracting the tricuspid RegVol from the total RVSV. The end point was a composite of all-cause death and heart failure hospitalization. Results: After a mean follow-up of 18 ± 15 months, 195 patients (38%) reached the composite end point. At time-dependent receiver operating characteristic analysis, eRVEF (area under the curve [AUC] = 0.72; 95% CI, 0.68-0.77) showed a stronger association with outcome than RVEF (AUC = 0.65; 95% CI, 0.59-0.70; P = .006), tricuspid annular plane systolic excursion (AUC = 0.64; 95% CI, 0.59-0.69; P = .01), RV free-wall longitudinal strain (AUC = 0.63; 95% CI, 0.58-0.68; P = .003), and RV fractional area change (AUC = 0.55; 95% CI, 0.50-0.60; P < .001). The eRVEF cutoff associated with an excess event rate was 20% on spline curve modeling. Patients with eRVEF <20% demonstrated a higher rate of events at 2 years (65% ± 6%) than those having an eRVEF ≥20% (22% ± 7%, log-rank <0.0001). An eRVEF <20% was associated with a 3-fold increased risk of experiencing the composite end point (hazard ratio = 3.54 [2.61-4.79], P < .001). On different models of multivariable analysis, eRVEF as a continuous variable remained independently associated with the combined end point (hazard ratio = 0.96; 95% CI, 0.94-0.98; P < .001). Conclusions: In patients with STR, eRVEF was more closely associated with all-cause mortality and heart failure hospitalizations than RVEF and other conventional echocardiographic indices of RV function
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