834 research outputs found
Adaptation, reliability and validity of health-related quality of life questionnaires: disabkids chronic and specific diabetes disease in children and adolescents with diabetes mellitus type 1
No full textAlonso Rubio, P., Bahíllo Curieses, M.P., Prieto Matos, P., Bertholt Zuber, M.L., Martín Alonso, M.M., Murillo Vallés, M., Chueca Guindulain, M.J., Berrade Zubiri, S., Huidobro Fernández, B., Prado Carro, A.M., Rodríguez Estévez, A., Rica Echevarría, I., Grau Bolado, G., Díez López, I., Fernández Ramos, M.C., Rodríguez Dehli, C., Riaño-Galán, I
Functional traits in lichen ecology: A review of challenge and opportunity
No full textCommunity ecology has experienced a major transition, from a focus on patterns in taxonomic composition, to revealing the processes underlying community assembly through the analysis of species functional traits. The power of the functional trait approach is its generality, predictive capacity such as with respect to environmental change, and, through linkage of response and effect traits, the synthesis of community assembly with ecosystem function and services. Lichens are a potentially rich source of information about how traits govern community structure and function, thereby creating opportunity to better integrate lichens into ‘mainstream’ ecological studies, while lichen ecology and conservation can also benefit from using the trait approach as an investigative tool. This paper brings together a range of author perspectives to review the use of traits in lichenology, particularly with respect to European ecosystems from the Mediterranean to the Arctic-Alpine. It emphasizes the types of traits that lichenologists have used in their studies, both response and effect, the bundling of traits towards the evolution of life-history strategies, and the critical importance of scale (both spatial and temporal) in functional trait ecology
Excreción urinaria de calcio en niños sanos de Salamanca: parámetros urinarios, valores de referencia y sintomatología
No full text[ES]Introducción: La calciuria en niños está influenciada por determinados factores, tanto intrínsecos como extrínsecos. Lo más apropiado sería disponer de valores de referencia para cada población. La valoración de la calciuria mediante el índice calcio/ creatinina (Ca/Cr) en niños está marcada por una falta de estandarización.
Objetivo: Estudiar la calciuria en la población infantil salmantina mediante los índices Ca/Cr y Calcio/osmolaridad (Ca/Osm) en orina de micción aislada y establecer valores de referencia, diferentes factores influyentes y significación clínica.
Material y métodos: Estudio transversal. Se obtienen muestras de micción aislada de 496 niños/as (6-12 años) de dos colegios de la provincia de Salamanca. Se registran factores extrínsecos e intrínsecos influyentes en la calciuria, y condiciones previas a la recogida de la muestra (2 encuestas tipo test). Se analizan índices Ca/Cr, Ca/Osm, Na/K y otros parámetros urinarios.
Resultados: Participaron un total de 496 niños (9.35 ±1.71 años; 50.4% niñas). No existen diferencias en la calciuria, analizada con el índice Ca/Cr y Ca/Osm en relación al sexo, edad, AFde litiasis, ingesta habitual de lácteos, verdura y fruta, clínica miccional, abdominalgia recurrente, hematuria, fracturas, ni el lugar que ocupaba la orina recogida (p>0.05). La mediana de Ca/Cr fue 0.13 mg/mg y Ca/Osm 0.011 mg/l/mOsm/kg; existiendo correlación positiva entre Ca/Cr, Ca/Osm (r0.914 y r2 0.786; p<0.001) y Na/K. El índice Ca/Cr de 0.21 mg/mg corresponde al p75 de nuestra muestra. El punto de corte a partir del cual aparece significación clínica, en forma de AP de ITU, es para el índice Ca/Cr ¿0.34 mg/mg (p95) y para el Ca/osm ¿ 0.027 mg/l/mOsm/kg (p97).
Conclusiones: El Ca/Cr no varía en función de la micción analizada, ni de la ingesta reciente ni habitual de lácteos. El Ca/Osm tiene una fuerte correlación con Ca/Cr, pero no aporta ventajas significativas sobre el índice Ca/Cr para valorar la calciuria al estar más influenciado por la ingesta previa y ser más caro. Se propone como valor de referencia para el diagnóstico de hipercalciuria en nuestra población un Ca/Cr 0.34 (p95), ya que es el valor a partir del cual aparece significación clínica; estimando una prevalencia de hipercalciuria de 4.8%
Paguristes candelae Matos-Pita & Ramil, 2015, n. sp.
No full textPaguristes candelae n. sp. (Fig. 7–12) Material examined. Holotype: Stn. MU 140 (17 ° 39 ' 25 "N, 16 ° 38 ' 11 "W to 17 ° 42 ' 28 "N, 16 ° 38 '00"W), 376–377 m, 4 November 2008 (MNCN 20.04 / 9822), male, sl 9.54 mm, entire, part of the anterior right branchiostegite is missing, inhabiting a shell of Euthriostoma saharicum (Locard, 1897) attached with two specimens of an unidentified actinia. Paratypes: Stn. MUBV 18 (18 ° 28 ' 27 " N, 16 ° 42 ' 43 "W to 18 ° 28 ' 14 "N, 16 ° 42 ' 40 "W), 559–574 m, 11 December 2009 (MNCN 20.04 / 9823), 1 female, sl 5.10 mm, entire, lacking right pereiopod 4 dactylus and half propodus, without a shell; Stn MUDR 12 (19 ° 52 ' 38 "N, 17 ° 22 ' 23 "W), 485 m, 26 November 2010 (UVIGOBA 3 –02500), 1 female, sl 3.91 mm, entire, right P 3 and left P 2 detached, inhabiting a shell of Nassarius wolffi (Knudsen, 1956) (specimen added posteriorly, collected in the same area with a rock dredge). Description. Shield slightly longer than broad (1.04 times); anterolateral margins weakly sloping, one spine on anterolateral angle; anterior margin between rostrum and lateral projections concave; posterior margin truncated; dorsal surface rugose with low, irregular elevations on gastric region on either side of the midline and scattered tufts of long simple setae laterally (Fig. 8 A). Rostrum short, broadly triangular, with a terminal thin spine weakly curved ventrally, falling short in relation to the lateral projections; dorsal surface with a median elevation; lateral margins smooth and furnished with long plumose setae. Lateral projections subtriangular, with small and weakly developed marginal blunt spines on the left, not developed on the right (Fig. 8 B). Branchiostegites strongly calcified (Figs. 7 A, B), anterior margin smooth and furnished with long plumose setae on the left (right is missing); covering 13 pairs of biserial phyllobranchiae; pleurobranchiae above pereiopods 2–4. Ocular peduncles moderately slender (5.85 times longer than the width of cornea), about 0.54 times as long as shield, cylindrical, weakly inflated basally, cornea not dilated; both dorsal surfaces with dorsolateral tufts of long simple setae proximally and a longitudinal dorsomesial row of long simple setae (Fig. 8 B). Ocular acicles subtriangular, ending in a simple spine on the left and with an additional small spine behind the main one (Fig. 8 B); mesial and lateral margins unarmed; mesial margin furnished with short plumose setae; separated by 0.3 basal width of one acicle. Antennular peduncles, when fully extended, overreach distal margins of corneas by 0.8 length of ultimate segment. Ultimate segment with a dorsal longitudinal row of scattered long, simple setae; penultimate segment without setae. Basal segment with spine on dorsolateral margin of statocyst lobe, laterodistal margin with spinule, ventromesial distal angle produced, ending in acute spine. Antennal peduncles, when fully extended, overreach cornea by 1 / 3 the length of the fifth segment; fifth segment unarmed; fourth segment with a small spine at dorsodistal margin; third segment with ventromesial distal angle strongly produced, ending in a strong spine; second segment with dorsolateral distal margin produced, ending in three spines almost concealed by a tuft of long simple setae, lateral margin with a small spine on the left antenna and smooth on the right one, dorsomesial angle with two distal spines, mesial row of short plumose setae; first segment with a very small laterodistal marginal spine on the left antenna, unarmed on the right one. Antennal acicles moderately long, slightly falling short of the distal margin of the ultimate peduncular segment, with numerous long simple setae; ending in a strong bifid spine; inflated basally; mesial margin with eight (left) or seven (right) strong spines, lateral margin armed distally with two (right) or three (left) strong spines (Fig. 8 B). Antennal flagella three times the length of the shield, slightly exceeding the tips of chelipeds, composed of about 50 articles, each with simple setae on their distal margin; setae up to six articles long. Third maxilliped ischium with well-developed crista dentata composed of moderately sized corneous teeth, without accessory tooth, ventrodistal margin and dorsolateral corner with one spine; merus with three moderate and separated ventral spines, one spine on dorsodistal margin; carpus with one spine on dorsodistal margin; dactylus shorter than propodus (Fig. 10 D). Chelipeds subequal and with similar armature, left slightly large and longer than right (Fig. 7 C). Dactylus 1.5 times longer than palm, cutting edge with a row of small calcareous teeth in proximal 0.7 and a row of strong corneous teeth in distal 0.3, ending in a strong corneous claw, overlapped by a fixed finger; dorsomesial margin with an irregular row of moderately small, conical, corneous-tipped spines, decreasing in size distally, dorsal surface with scattered tufts of long stiff setae and a couple of spinulose tubercles proximally; mesial face protuberant ventrally (Fig. 10 B) with scattered tufts of short setae and corneous, or only corneous-tipped, spinules; ventral surface unarmed, with tufts of stiff setae. Palm slightly shorter than carpus (Fig. 10 A); dorsomesial margin with three strong, corneous-tipped spines, dorsal surface convex, without delineation of dorsolateral margin, with several irregular rows of moderately strong, corneous-tipped spines, decreasing in size on the fixed finger, and with a tuft of stiff setae; mesial surface with a couple of low protuberances distally, with tufts of long setae, mesiodistal margin unarmed; ventral surface well inflated, with row of spinulose tubercles with tufts of long setae along midline, extending onto the fixed finger, and few protuberances or tubercles laterally and mesially. Fixed finger not noticeably deflexed (Fig. 10 B); dorsal surface with irregular rows of moderately strong corneous-tipped spines, decreasing in size distally; lateral margin not clearly delineated; cutting edge with row of small calcareous teeth in proximal 0.7 and row of strong corneous teeth in distal 0.3, ending in a strong corneous claw; a narrow hiatus when the claw is closed. Carpus about 0.6 times as long as merus (Fig. 10 B); dorsomesial margin with an irregular row of four or five strong, conical, corneous-tipped spines, increasing in size distally; dorsal surface with single or double row of moderately strong, corneous-tipped spines at both sides of the midline and scattered tufts of long single setae; dorsodistal margin produced, with moderately small corneus-tipped spine laterally; dorsolateral margin not clearly delineated; mesial surface with tufts of setae along dorsal and distal margins; lateral surface with some scattered and moderately small tubercles, with tufts of setae; laterodistal margin with few small spines dorsally; ventral face unarmed. Merus moderately deep; dorsal surface with a row of small spinulose tubercles with tufts of long simple setae; dorsodistal margin weakly spinulose, with a spinulose transverse ridge running subdistally from lateral to mesial faces; mesial surface smooth; ventromesial margin with a row of moderately strong, corneoustipped spines and sparse simple setae; lateral surface with scattered small tubercles and few short setae (Fig. 10 B); ventral face unarmed and with a row of plumose setae distally following the ventrolateral margin; ventrolateral margin with small spine near distal corner. Ischium with a row of small spinulose tubercles, increasing in size distally, furnished with plumose setae on ventromesial margin, ventrolateral distal angle with a couple of small spines. Coxa unarmed. Second pereiopods overreaching chelipeds by about the total length of the dactylus (Figs. 7 A, 9 A). Dactylus about 0.6 times longer than propodus; weakly curved in lateral view, nearly straight or slightly twisted in dorsal view; ending in a strong, curved, corneous claw; each dorsal surface with a row of small to moderately small corneous-tipped spines, becoming smaller distally, partially obscured by numerous tufts of long setae; each mesial face with two rows of tufts of setae dorsally and ventrally (Fig. 10 C); lateral faces with two rows of sparse tufts of setae subdorsally and subventrally; each ventral margin with a row of about 30 small corneous spines anteriorly directed, increasing in size distally. Propodus distinctly longer than carpus; dorsal surfaces each with a more or less irregular row of strong, corneous-tipped spines mesially, less numerous on left pereiopod, partially obscured by tufts of long setae; dorsodistal margins with a spine; mesial surfaces with two entire rows of tufts of long setae dorsally and ventrally and one short row of scattered tufts of long setae on the proximal midline; lateral surfaces unarmed, each one with a row of tufts of long setae near the dorsal and ventral margins and also on the midline; ventral surfaces unarmed, each with an irregular row of tufts of long setae. Carpus dorsal surface with a row of strong, corneous-tipped spines mesially and tufts of long setae (Fig. 10 C), dorsodistal margin with a strong corneus-tipped spine; mesial surfaces smooth; lateral faces convex, each one with shallow longitudinal sulcus irregularly lined with scattered tufts of long simple setae, median scattered tufts of long simple setae and one row of lateroventral tufts of long simple setae; smooth ventral surfaces with a couple of tufts of short simple setae; ventrodistal margins with some long simple setae and a few short plumose setae. Merus strongly compressed laterally; each dorsal surface with a row of tufts of long simple setae; mesial and lateral surfaces smooth; each ventral surface with a mesial row of small spines or spinules and numerous long simple setae with some short plumose setae, ventrolateral angle furnished with two short plumose setae. Ischium dorsal surfaces with a row of spinules and some short plumose setae, dorsodistal margins with a short spine; ventral margins with a distal row of long plumose setae. Coxa unarmed other than with small spines on ventrolateral and ventromesial distal angles. Third pereiopods mostly similar to second in setation (Figs. 7 A, 9 A). Dactylus with a proximal single row of small corneous-tipped spines and small corneous spines distally on dorsomesial margin; each mesial surface with a single or a double row of small corneous spines ventral to midline (Fig. 10 C); each ventral surface with a row of about 30 small corneous spines anteriorly directed, flanked by two rows of tufts of long setae. Propodus unarmed. Carpus with a dorsal row of 2 or 3 spines proximally and a subdistal dorsal spine; mesial faces with one mediodistal tuft of setae (Fig. 10 C). Merus unarmed apart from a few spinules proximally on dorsal surfaces. Ischium with some barely noticeable spinules on dorsal surface and a very small subdistal spinous low tubercle. Coxa unarmed on dorsal and ventral margins. Fourth pereiopods setose on dorsal and ventral margins. Dactylus weakly curved, ending in a strong corneous claw, without preungal process (Fig. 10 E); dorsal surface unarmed; ventral surface with a lateral row extending to 0.7–0.8 length of ventral margin and composed of 6 or 7 corneous teeth. Propodal rasp formed by 5–6 rows of ovate scales (Fig. 10 E). Carpus and merus unarmed. Fifth pereiopods chelate, setose. Dactylus covered with ovate scales (Fig. 10 F). Propodus with a welldeveloped rasp (Fig. 10 F). Each coxa with a gonopore. Pleopods. First and second pleopods paired and modified (Figs. 11 A, 11 B). First pleopod robust, inferior lamella with the distal rounded margin furnished with 2–3 rows of hook-like spines (Fig. 11 C); external lobe with a curved tip and separated from the internal lobe by a wide rounded notch (Fig. 11 C); broad internal lobe with long setae distally (Figs. 11 A, 11 C). Second pleopod uniramous (Fig. 11 D). Third to fifth pleopods unpaired, exopods very well developed, endopods rudimentary. Abdominal tergites. Second and third abdominal tergites moderately calcified on their left (Fig. 7 B), with long plumose setae on the left margins; fourth abdominal tergite widely separated from the third tergite, with moderately long plumose setae. Uropods strongly asymmetrical (Fig. 9 D); each protopod with a few small corneous-tipped spines on its posteroventral margin. Telson with posterior lobes somewhat asymmetrical, left lobe larger than the right; broadly rounded; separated by a wide median cleft; terminal margins with short broad spines, 7 (left) and 6 (right) (Fig. 11 E); deep transverse indentations (as in Fig. 9 D); anterior lobes unarmed on their lateral margins. Females. Differences other than those attributed to sex: relative length of the antennal penduncle that falls short of distal cornea; absence of subterminal spine in the ocular acicles (Fig. 9 B); spines on ventrolateral and ventromesial distal angles on pereiopod 2, coxae almost inconspicuous; pereiopods 2 and 3 with the dactylus ventral margin furnished with fewer anteriorly directed corneus spines (about 20); pereiopod 4 with only five corneus teeth in the dactylus ventrolateral row; and telson with nine thinner marginal teeth on each lobe (Fig. 9 D). Females show paired gonopores (Fig. 12 A); first pleopods paired, uniramous; second to fifth pleopods unpaired, second to fourth with both rami well developed, exopods much longer than endopods and fifth pleopod shorter, with the exopod well developed and a vestigial endopod. Brood pouch moderately large, subtriangular, with smooth margins furnished with long plumose setae (Fig. 12 B). Etymology. Devoted to Candela, the daughter of the first author. Remarks. To date 18 species have been assigned to Paguristes in West Africa, seven of which were transferred to Areopaguristes (Rahayu 2005, present work) and one to Pseudopaguristes (present work). The type material of the other ten species must be checked in order to clarify their taxonomic status, as suggested by McLaughlin (2002) and Rahayu (2005). There are no indications of the gill number in the other ten Paguristes species in the literature, but P. candelae n. sp can be clearly differentiated by other features, including the multidentate ocular acicles (more than 3 spines) of Paguristes fagei Forest, 1952, Paguristes insularis Forest, 1966, Paguristes microphthalmus Forest, 1952, Paguristes oxyacanthus Forest, 1952 and Paguristes skoogi Odhner, 1923; the relative length of the rostrum, the antennal acicle and the antennal peduncle, as well as the morphology of chelipeds, and pereiopods 2 and 3 of the species Paguristes agulhasensis Forest, 1954 a, Paguristes barnardi Forest, 1954 a, Paguristes gamianus (H. Milne-Edwards, 1836), Paguristes macrotrichus Forest, 1954 a and Paguristes rubropictus A. Milne-Edwards & Bouvier, 1892. Moreover, the presence of two gonopods in females was only described for P. gamianus and P. rubropictus, although this detail is unknown for P. agulhasensis (only one male was ever reported) and for P. barnardi, P. macrotrichus and P. skoogi because this feature was never specified; in the other species, females have only one gonopore on left third pereiopod. Paguristes eremita (Linnaeus, 1767), Paguristes streaensis Pastore, 1984 and Paguristes syrtensis de Saint Laurent, 1971, also reported from E Atlantic and the Mediterranean Sea, have a rostrum that exceeds the lateral projections and granular chelipeds, whereas in Paguristes candelae n. sp. the rostrum falls short of the lateral projections and the chelipeds have strong corneus-tipped spines. We also checked the descriptions of the 31 species currently referred to as Paguristes sensu lato from the western Atlantic Ocean (A. Milne-Edwards 1880; A. Milne-Edwards & Bouvier 1893; Benedict 1901; Schmitt 1933; Forest 1954 b; Holthuis 1959; Provenzano 1965; McLaughlin & Provenzano Jr. 1974, 1975; Campos & Sánchez 1995; Sandberg, 1996; Manjón-Cabeza et al. 2002); none of them concurred with the above-mentioned combination of features observed in our specimen, related to the rostrum, antennal acicles, cheliped morphology and telson armature.Published as part of De Matos-Pita, Susana S. & Ramil, Fran, 2015, Hermit crabs (Decapoda: Crustacea) from deep Mauritanian waters (NW Africa) with the description of a new species, pp. 151-190 in Zootaxa 3926 (2) on pages 164-170, DOI: 10.11646/zootaxa.3926.2.1, http://zenodo.org/record/24219
Genetic variants in the exome as responsible for rare diseases in pediatrics
No full textTrabajo de fin de grado. Grado en Medicina. Curso académico 2023-2024[ES]Introducción. La secuenciación masiva de nueva generación, o NGS, es un conjunto de técnicas moleculares que permiten identificar rápidamente el orden de los nucleótidos, siendo útil para detectar variantes genéticas. La secuenciación del exoma completo (WES) es una técnica de NGS que ha cobrado gran importancia en los últimos años, especialmente en enfermedades muy heterogéneas, como las enfermedades raras, siendo útil en enfermedades como trastornos del neurodesarrollo, talla baja o anomalías congénitas. Aunque tiene ventajas en rapidez y coste, su complejo análisis bioinformático y la limitación en la detección de ciertas alteraciones son desafíos importantes.
Objetivos. Estudiar la utilidad de la secuenciación del exoma completo en el diagnóstico de las enfermedades raras en pediatría.
Material y métodos. Se diseñó un estudio observacional retrospectivo realizado en pacientes pediátricos con sospecha de enfermedades raras genéticas derivados a la Unidad de Referencia de Diagnóstico Avanzado de Enfermedades Raras de Castilla y León (DiERCyL) a los que se les realizó una prueba genética de WES en los años 2019 y 2020. Resultados. Se analizan 438 pacientes con estudio de WES, con una edad de 10,76±4,97 años, siendo el 39,5% mujeres, donde el grupo de edad mayoritario es el de 10-15 años. Se reciben pacientes de todas las provincias siendo las que más derivan Burgos (31,6%) Salamanca (21,1%) y León (11,9%). Las especialidades que precisan con más frecuencia la realización de WES son neurología (47,7%) y endocrinología (23,8%). La prevalencia de características clínicas de los pacientes varía, siendo el retraso del lenguaje la más común (36,6%). El rendimiento diagnóstico de la WES en este estudio es del 52,1% sin demostrarse diferencias ni por provincias ni por especialidad. Por el contrario, se demuestran diferencias en la positividad de la prueba en relación con la alteración del fenotipo (p<0,001), microcefalia (p=0,001) y otros trastornos neurológicos. Las variantes puntuales más frecuentes son las missense (54,1%), teniendo el 78,6% de todas las variantes una interpretación patogénica o probablemente patogénica. De los 124 pacientes a los que se realiza estudio de progenitores, 54 son variantes de novo. Solo se describen 2 variantes del número de copias. Los genes NF1, PTPN11 y SCN1A son los que más frecuentemente se alteran. Conclusiones. DiERCyL recibe pacientes de todas las provincias y especialidades de pediatría, con diferentes tasas diagnósticas, pero en conjunto, con un rendimiento diagnóstico superior al encontrado en la literatura. Las variantes más frecuentes son las variantes puntuales de tipo missense, de significado patogénico y probablemente patogénico, siendo infrecuente la detección de variantes del número de copias. Se ha encontrado que la presencia de determinadas características clínicas aumenta el riesgo de resultados concluyentes.[EN]Introduction. Next-generation sequencing (NGS) is a set of molecular techniques that allow for the rapid identification of nucleotide order, proving useful in detecting genetic variants. Whole exome sequencing (WES) is an NGS technique that has gained significant importance in recent years, especially in highly heterogeneous diseases such as rare diseases, proving helpful in conditions such as neurodevelopmental disorders, short stature, or congenital anomalies. Despite its advantages in speed and cost, its complex bioinformatic analysis and limitation in detecting certain alterations pose significant challenges. Objectives. To study the usefulness of whole exome sequencing in the diagnosis of rare diseases in pediatrics. Materials and methods. An observational retrospective study was designed, conducted on pediatric patients with suspected genetic rare diseases referred to the Reference Unit for Advanced Diagnosis of Rare Diseases of Castilla and León (DiERCyL) who underwent WES genetic testing in the years 2019 and 2020. Results. A total of 438 patients with WES studies were analyzed, with a mean age of 10.76±4.97 years, of which 39.5% were females, with the majority in the age group of 10-15 years. Patients were received from all provinces, with Burgos (31.6%), Salamanca (21.1%), and León (11.9%) being the provinces with the highest referral rates. The specialties requiring WES testing most frequently were neurology (47.7%) and endocrinology (23.8%). The prevalence of clinical characteristics varied, with language delay being the most common (36.6%). The diagnostic yield of WES in this study was 52.1%, with no differences demonstrated by province or specialty. However, differences were observed in test positivity related to phenotype alteration (p<0.001), microcephaly (p=0.001), and other neurological disorders. The most frequent single nucleotide variants were missense mutations (54.1%), with 78.6% of all variants having a pathogenic or likely pathogenic interpretation. Out of 124 patients who underwent parental studies, 54 had de novo variants. Only 2 copy number variants were described. The genes NF1, PTPN11, and SCN1A were the most frequently altered. Conclusions. DiERCyL receives patients from all provinces and pediatric specialties, with different diagnostic rates, but overall, with a diagnostic yield superior to that found in the literature. The most frequent variants are missense mutations, with pathogenic or likely pathogenic significance, and the detection of copy number variants is infrequent. It has been found that the presence of certain clinical characteristics increases the risk of conclusive results
Rational identification of a colorectal cancer targeting peptide through phage display
No full textColorectal cancer is frequently diagnosed at an advanced stage due to the absence of early clinical indicators. Hence, the identification of new targeting molecules is crucial for an early detection and development of targeted therapies. This study aimed to identify and characterize novel peptides specific for the colorectal cancer cell line RKO using a phage-displayed peptide library. After four rounds of selection plus a negative step with normal colorectal cells, CCD-841-CoN, there was an obvious phage enrichment that specifically bound to RKO cells. Cell-based enzyme-linked immunosorbent assay (ELISA) was performed to assess the most specific peptides leading to the selection of the peptide sequence CPKSNNGVC. Through fluorescence microscopy and cytometry, the synthetic peptide RKOpep was shown to specifically bind to RKO cells, as well as to other human colorectal cancer cells including Caco-2, HCT 116 and HCT-15, but not to the normal non-cancer cells. Moreover, it was shown that RKOpep specifically targeted human colorectal cancer cell tissues. A bioinformatics analysis suggested that the RKOpep targets the monocarboxylate transporter 1, which has been implicated in colorectal cancer progression and prognosis, proven through gene knockdown approaches and shown by immunocytochemistry co-localization studies. The peptide herein identified can be a potential candidate for targeted therapies for colorectal cancer.Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and the Project FCOMP-01–0124-FEDER-021053 (PTDC/SAU-BMA/121028/2010). Débora Ferreira is recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15) funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Franklin L. Nobrega, Sara Granja and Ligia R Rodrigues acknowledge FCT for the grants SFRH/BD/86462/2012, SFRH/BPD/117858/2016 and SFRH/BSAB/142991/2018, respectively. Catarina Barbosa-Matos also acknowledge her research grant UMINHO/BI/395/2018info:eu-repo/semantics/publishedVersio
Trabajos arqueológicos en Ajacuba, Hgo.: Primera aproximación
No full textTesis para optar por el grado de licenciatura en Arqueología y maestría en Ciencias Antropológicas</p
Paul et virginie: paratextos e textos em traduções brasileiras nos séculos XX e XXI
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-Graduação em Estudos da Tradução, Florianópolis, 2014.A presente tese tem como objetivo principal uma proposta de retradução comentada do romance Paut et Virginie do escritor francês Bernardin de Saint-Pierre. Como objetivo secundário, propõe-se uma análise das traduções brasileiras deste romance de 1906 à 2008 no sistema literário brasileiro sob o aspecto da "Visualização das Traduções" bem como um estudo dos paratextos traduzidos e não traduzidos. Tanto para a análise tradutória quanto para a retradução, são estudados trechos representativos a partir da teoria de Antoine Berman (1995), Gerard Genette (2010), Lawrence Venuti (1995). A análise dos excertos de narrativa poética baseia-se principalmente na teoria de Jean-Yves Tadié (1994).Abstract : The present work aims at proposing a commented retranslation on the novel Paul et Virginie, by the French author Bernardin de Saint-Pierre. As a secondary objective, we propose an analysis of the Brazilian translations of this novel from 1906 to 2008 into the Brazilian literary system under the perspective of "View of Translations" and a study of the translated and not translated paratexts. For both the translational analysis and the retranslation we used representative excerpts from the theoretical principles of Antoine Berman (1995), Gerard Genette (2010), and Lawrence Venuti (1995). The analysis of the excerpts of the poetic narrative is based mainly on Jean-Yves Tadié's theory
Mappings Between Banach Spaces That Preserve Convergence Of Series
No full textWe know that a numerical series is absolutely convergent, if, and only if, it is unconditionally convergent. Dvoretzky and Rogers proved in 1950 that in any infinite dimensional Banach space there are unconditionally convergent series not absolutely convergent. This result was the origin of the development of the study of the linear mappings between Banach spaces sending unconditionally summable sequences into absolutely summable sequences (the Theory of absolutely Summing Mappings). The Nonlinear Theory started with Pietsch in 1983, when he presented a few results for scalar multilinear mappings and homogeneous polynomials defined on Banach spaces. In 1989 this author started the study of absolutely summing holomorphic mappings between Banach spaces. In a minicourse, thaught in 1997 at a Seminário Brasileiro de Análise, this author presented several results dated of 1996 and 1997 on nonlinear absolutely summing mappings (not necessarily holomorphic mappings) between Banach spaces. This course included an interesting characterization of regularly summing mappings f between Banach spaces, that is those mappings such that (f(a + xj) - f(a))∞ j=1 is absolutely summable whenever (xj)∞ j=1 is absolutely summable. This result, applied in a convenient setting, implied a nice characterization result for nonlinear absolutely summing mappings, bearing striking similarities to the correspond result for linear absolutely summing mappings. These and other results, as well as historical references, can be found in M. C. Matos, Math. Nachr. 258, 71-89 (2003). In this work we introduce and develop the concept of uniformly regularly summing mappings between Banach spaces, allowing an interesting characterization of the uniformly absolutely summing mappings between Banach spaces, thus extending previous results on nolinear absolutely summing mappings. We also state a relation between uniform regularity and the Lipschitz property for mappings between Banach spaces.2527796Aron, R., Globvenik, J., Analytic functions on c0 (1989) Revista Math., 2, pp. 27-33Grothendieck, G., Resumé de la théorie métrique des produits tensorielstopologiques (1956) Bol. Soc. Mat. São Paulo, 8, pp. 1-79Kwapien, S., On a theorem of L. Schwartz and its applications to absolutely summing operators (1970) Studia Math., 38, pp. 193-201Lidenstrauss, J., Pelczynski, A., Absolutely summing operators in Lp-spaces and applications (1968) Studia Math., 29, pp. 275-326Matos, M.C., Nonlinear absolutely summing mappings (2003) Math. Nachr., 258, pp. 71-89Nachbin, L., Topologies on spaces of holomorphic mappings (1969) Ergebn. Math. Grenzgeb., p. 47. , Springer-VerlagSchwartz, L., Probabilités cylindriques et applications radonifiantes (1969) C .R. Acad. Paris, 268, pp. 646-64
Templo Mayor: Distrito Federal
No full textEs el principal templo perteneciente al recinto ceremonial de Tenochtitlan, descubierto entre 1978 y 1982, donde había hasta 78 edificios. El Templo Mayor se caracteriza por haber tenido cuatro cuerpos superpuestos sobre una plataforma general; dos escalinatas orientadas al poniente daban acceso a la parte alta, que constaba de dos adoratorios: uno dedicado al dios de la guerra, Huitzilopochtli y el otro, al dios del agua, Tláloc. La presencia de estos dioses obedece a las necesidades fundamentales del pueblo azteca o mexica: la guerra como medio de expansión y control económico de otras regiones, a las que se les imponía un tributo; el agua como elemento natural fundamental, puesto que se trataba de un grupo agricultor que había desarrollado técnicas importantes. Es evidente que la presencia de ambos dioses estaba en relación directa con la sobrevivencia del pueblo.</p
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