1,319 research outputs found
Analysis of phenolic compounds and radical scavenging activity of Echinacea spp
No full textThe aim of this study was to set up and validate an RP-LC method with DAD-detection to quantify caffeic acid derivatives in various Echinacea spp. Samples were extracted with 80% methanol. The analyses were carried out on a Lichrospher RP-18 column (125 mm x 4 mm i.d., 5 microm), with a mobile phase gradient, which increases the acetonitrile level in a phosphoric acid solution (0.1%). The flow rate was 1.5 ml/min. Detection was set at 330 nm. This method allowed the identification and quantification of caftaric acid, chlorogenic acid, caffeic acid, cynarin, echinacoside and cichoric acid in Echinacea roots and derivatives. The total phenolic content was 10.49 mg/g for E. angustifolia, 17.83 mg/g for E. pallida and 23.23 mg/g for E. purpurea. Among Echinacea commercial herbal medicines, a certain variability in the concentrations of phenolic compounds was observed. The radical scavenging activity of Echinacea methanolic extracts was evaluated in vitro with a spectrophotometric method based on the reduction of an alcoholic 2,2-diphenyl-1-picrylhydrazyl (DPPH*) radical solution at 517 nm in the presence of a hydrogen donating antioxidant. As for pure compounds, echinacoside had the highest capacity to quench DPPH* radicals (EC50 = 6.6 microM), while caftaric acid had the lowest (EC50 = 20.5 microM). The average EC50 values for E. purpurea, E. pallida and E. angustifolia were 134, 167 and 231 microg/ml, respectively. The radical scavenging activity of Echinacea root extracts reflected their phenolic composition. The results indicate that Echinacea roots and derivatives are a good source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects
Inleiding in de waterbouwkunde f1
No full text1. inleiding 2. kanalen 3. rivieren 4. schutsluizen en dergelijke 5. overige kunstwerken 6. oceanen en zeeën 7. totaaloverzichtHydraulic EngineeringCivil Engineering and Geoscience
The coupled chemomechanics of the F1-ATPase molecular motor
No full textAbstractThe enzyme F1-ATPase is a rotary nanomotor in which the central γ subunit rotates inside the cavity made of α3β3 subunits. The experiments showed that the rotation proceeds in steps of 120° and each 120° step consists of 80° and 40° substeps. Here the Author proposes a stochastic wave mechanics of the F1-ATPase motor and combines it with the structure-based kinetics of the F1-ATPase to form a chemomechanic coupled model. The model can reproduce quantitatively and explain the experimental observations about the F1 motor. Using the model, several rate-limited situations about γ subunit rotation are proposed, the effects of the friction and the load on the substeps are investigated and the chemomechanic coupled time during ATP hydrolysis cycle is determined
Excess heat capacity of the (Li1?xCax)F1+x liquid solution determined by differential scanning calorimetry and drop calorimetry
No full textThe work presents the measured heat capacity of the (Li1?xCax)F1+x liquid solution. Four samples with different compositions have been prepared and measured using a Differential Scanning Calorimeter. Since this technique was newly adopted for measuring encapsulated fluoride samples, some modifications were introduced in the standard configuration of the instrument and they are described in this work as well. For comparison one of the analysed composition (xCaF2xCaF2 = 0.5) was also measured using drop calorimetry, which has been previously used for similar studies. The reliability of the results obtained was confirmed by the good agreement between the two techniques. Moreover, the excess heat capacity of the (Li1?xCax)F1+x liquid solution was derived and a strong deviation from the ideal behaviour was observed.RST/Radiation, Science and TechnologyApplied Science
Tackling Different Velocity Borne Challenges of the Elastodynamic Marchenko Method
No full textThe elastodynamic Marchenko method removes overburden interactions obscuring the target information. This method either relies on separability of the so-called focusing and Green's functions or requires an accurate initial estimate of the focusing and Green's function overlap. Hitherto, F1- and G-+ have been assumed separable, whereas F1+ and (G-)* share an unavoidable overlap, which has been considered understood but hard to predict without knowing the model. However, velocity differences between P- and S-waves cause so far unexplored fundamental challenges for elastodynamic Marchenko autofocusing. These challenges are analysed for horizontally-layered media. First, the F1-/G-+ separability assumption can be violated depending on the medium, the redatuming depth and the angle of incidence. Second, the initial estimate of the said unavoidable overlap can be even more complicated than originally thought, including some of the internal multiples. We propose a strategy where we trade-off this sophisticated initial estimate with a trivial one at the cost of a more restrictive F1-/G-+ separability assumption, or at the cost of introducing an overlap between F1- and G-+ instead. The proposed method finds the desired solutions convolved by an unknown matrix which we can hope to remove by exploiting energy conservation and minimum-phase properties of the focusing functions.Accepted author manuscriptApplied Geophysics and PetrophysicsQN/Theoretical PhysicsImPhys/Acoustical Wavefield Imagin
DIPLOMASI PUBLIK INDONESIA MELALUI PENYELENGGARAAN F1 POWERBOAT DANAU TOBA
No full textThis thesis is entitled Indonesian Public Diplomacy Through the Implementation of F1 Powerboat Lake Toba. This research analyzes how Indonesia's efforts in using public diplomacy in organizing F1 Powerboat on Lake Toba. With the presence of the F1 Powerboat event on Lake Toba, it is hoped that it can become a momentum for increasing the tourism economy in Lake Toba after the co-19 pandemic. This event is also expected to have a multiplier impact on the economy of the Lake Toba community, one of whose regional income is contributed through tourism economic activities. In this research, using a descriptive method with a qualitative analysis approach is considered capable of explaining Indonesia's public diplomacy efforts through organizing F1 Powerboat on Lake Toba. The concept used is public diplomacy by Mark Leonard, with the operationalization of the concept of 3 dimensions of public diplomacy, namely news management, strategic communications, and building relationships. With the concept of public diplomacy, the author analyzes how Indonesia's public diplomacy efforts through the implementation of F1 Powerboat Lake Toba
Gene expression profiling of Pseudomonas putida F1 after exposure to aromatic hydrocarbon in soil by using proteome analysis
No full textPseudomonas putida F1 can metabolize toluene, ethylbenzene, and benzene for growth. Previously, we identified proteins involved in the utilization of these compounds by P. putida F1 through culture in liquid media. However, it was unclear whether laboratory analysis of bacterial activity and catabolism accurately reflected the soil environment. We identified proteins involved in the degradation of toluene, ethylbenzene, and benzene growth in soil using two-dimensional gel electrophoresis (2-DE) or standard SDS-PAGE combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS). According to 2-DE/LC–MS/MS analysis, 12 of 22 key enzymes involved in the degradation of toluene, ethylbenzene, and benzene were detected. In standard SDS-PAGE/LC–MS/MS analysis of soil with ethylbenzene, approximately 1,260 cellular proteins were identified in P. putida F1. All key enzymes and transporter and sensor proteins involved in ethylbenzene degradation were up-regulated similar to that noted in liquid cultures. In P. putida F1, aromatic hydrocarbon response in soil is the same as that observed in liquid media
An Arcadian Toast (Harmod. FGrH 319 F1)
No full textInternational audienceThis paper deals with the transmitted text εδειπνιαις and εδειπνειας in a fragment of Harmodius of Lepreum (FGrH 319 F1 = Athenaeus 149b and 479c). Modern editors believe both εδειπνιαις and εδειπνειας to be a corruption of the genitive εδειπνας, which is accordingly interpreted as a toast meaning ‘a good appetite’. The author claims that this view must be rejected on several grounds. Other conjectures (ε δειπνεας, ε δεπνειας) are further analyzed and also discarded. Finally, it is shown that the only satisfactory reading is ε δειπνας = Att. ε δειπνσαις, ‘may you have a nice dinner’
The IN Vitro Cytotoxic effect of Toxoplasma GondII-Activated macrophages on F1 and F10 B16 melanoma cells, 1979
No full textNon-specific cytotoxicity, mediated by Toxoplasma gondii activated macrophages for the F1 and F10 B16 melanoma cells in vitro, was examined in this study. In vitro cytotoxicity was assayed by measuring the release of tritiated thymidine from pre-labeled tumor cells during twenty four-hour and seventy-two hour assay periods. Pre-labeled cells were incubated with peritoneal macrophages obtained from either uninfected C57BL mice used as controls or mice latently infected with the C56 strain of Toxoplasma gondii. There were significant differences observed in the total percent of tritiated thymidine released spontaneously by the F1 and F10 B16 melanoma cells compared to F1 and F10 melanoma cells incubated with macrophages obtained from either latently infected mice or uninfected mice during both assay periods used. There was no significant difference in the percent tritiated thymidine released by the F1 and F10 melanoma cells incubated with normal macrophages, compared to melanoma cells incubated with macrophages obtained from latently infected mice. When lysozyme activity was measured as an index of macrophage activation, the extracts of macrophages derived from mice with latent infection showed evidence of greater lysozyme activity than the extracts of macrophages obtained from uninfected mice. There is evidence in this study to show that although infection with Toxoplasma gondii did confer increased activation on macrophages, this degree of activation was not sufficient enough to demonstrate enhanced cytotoxic effect of the macrophages on the F1 and. F10 Bl6 melanoma tumor cells, under the conditions of the assays used in this study
Credo of a lucky textbook author <<Journal of Economic Perspectives>>, vol.11 no.2
No full textp.153-160コピーオリジナルの所在: 一橋大学経済研究所資料室都留重人氏寄贈1-F1-3-06
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