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PEMBENTUKAN VARIETAS SIRSAK UNGGUL MELALUI PEMULIAAN POLIPLOIDI: INDUKSI POLIPLOIDI TANAMAN SIRSAK DENGAN KOLKISIN
No full textPenelitian ini bertujuan membentuk sirsak unggul melalui serangkaian penelitian pemuliaan poliploidi secara bertahap; tahap pertama bertujuan mempelajari pengaruh perlakukan kolkisin terhadap fenotipe dan jumlah kromosom bibit sirsak sekaligus membentuk tanaman sirsak poliploid. Penelitian dilakukan melalui percobaan berulangan untuk menguji pengaruh perlakukan kolkisin (0,10%, 0,15%, dan kontrol/tanpa kolkisin) terhadap fenotipe bibit dan jumlah kromosom sirsak. Variabel-variabel yang diamati adalah tinggi tanaman, jumlah daun, dan kromosom. Hasil penelitian menunjukkan bahwa: (1) perlakukan kolkisin 0,1 % dan 0,15 % tidak berpengaruh terhadap tinggi tanaman dan jumlah daun pada pertumbuhan awal (vegetatif), belum terindentifikasi tanaman poliploid berdasar pengamatan fenotipe; (2) tanaman srikaya diploid mempunyai susunan kromosom (rumus kariotipe) 2n = 14 = 12 m + 2 sm atau n = 7 = 6 m + 1 m (terdiri atas 6 pasang kromosom metasentris, 1 pasang kromosom submetasentris)
ANALISIS POLA PITA-C KROMOSOM TANAMAN SALAK JANTAN DAN BETINA (Salacca zalacca var. zalacca)
Get PDFABSTRACT The objectives of this research were to study the C-banding pattern of male and female salak chromosome and to obtain the sex marker of salak based on C-banding pattern variation. C-banding pattern of male and female of salak were observed using geimsa C-banding technique. Variation of chromosomes C-banding pattern were analyzed according to number and position of C-band of chromosomes. The results of the research showed that C-band of salak chromosom can be revealed using geimsa C-banding technique. The C-band revealed on the telomer and the centromer region. Pro-metaphase and metaphase chromosomes could be used to observe the C-band. There were no differences in C-band pattern of chromosome of male and female salak, so the sex of salak can not be identified based on the observation of C-band of chromosome. Key words: chromosome, C-banding, sex marker, salak ABSTRAK Tujuan penelitian ini adalah mempelajari pola pita-C kromosom salak jantan dan betina, selanjutnya mengidentifikasi penanda kelamin tanaman salak berdasar perbedaan pola pita-C kromosom.Tanaman salak jantan dan betina varietas pondoh asal cangkok anakan digunakan untuk mempelajari perbedaan pola pita-C. Pengamatan pita-C kromosom dilakukan dengan teknik pemitaan geimsa (geimsa C-banding technique). Analisis perbedaan pola pita-C kromosom salak jantan dan betina dilakukan berdasar jumlah dan posisi pita-C dengan membandingkan karyogram dan idiogram pita-C. Hasil penelitian menunjukkan bahwa pita-C kromosom salak dapat dihasilkan menggunakan metode pemitaan-C geimsa (Geimsa C-banding technique), sebagian pita-C berada pada ujung kromosom atau terletak pada daerah telomer dan sebagian yang lain terletak di daerah sentromer. Pita-C dapat dihasilkan pada kromosom metafase awal (pro metaphase) maupun kromosom metafase (late metaphase). Tidak terdapat perbedaan pola pita-C antara kromosom tanaman salak jantan dan betina, sehingga jenis kelamin tanaman salak tidak didapatkan ditentukan berdasar pola pita-C kromosom. Kata kunci: kromosom, pita-C, penanda kelamin, salak
Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment
Get PDF Stevia rebaudiana Bert. (Stevia) is used in the commercial and health industries because of its steviol glycosides (stevioside, reÂbaudioside A, rebaudioside C) and flavonoids. Polyploidy induction of stevia plants using oryzalin was held to increase the diversity and produce superior varieties. This research aims to obtain stevia diverÂsity with different characteristics from its diploid. The TawangmanÂgu variety of stevia seedlings was experimentally designed using a completely random design. Nine combinations of oryzalin concentraÂtions (1.5, 2.5, and 3.5μM) and immersion times (4, 6, and 8 hours) treatments were applied to 15 stevia seedlings each. Stevia without treatment was used as a control. Treatments were applied directly to the shoots on the second internode from the tip. Observations on tarÂget shoots included the number of survivors, morphological variables (height, number of internodes, internode length, leaf size, leaf thickÂness, stem diameter, and leaf color), stomata, and plant ploidy level. Data analysis on the number of live plants used frequency and deÂscriptively. Morphological data consisting of plant height, number of shoots, number of internodes, internode length, leaf length, leaf width, leaf thickness, and stem diameter were analyzed using boxplot graphs and descriptive to describe the diversity of M1 stevia treatment reÂsults. Leaf color and stomata were analyzed descriptively. The results showed that up to 3.5μM concentrations of oryzalin and 6 hours of immersion time were safe to use as a mutation agent with above 67% survival rate. Various oryzalin treatments of Tawangmangu stevia vaÂrieties yielded polyploidy morphological growth indications in height, number of internodes, internode length, stem diameter, leaf size, leaf thickness, leaf color, stomata, and stem diameter. In addition, there are growth variations such as chimeras, rosettes, and leaf splitting. However, further flow cytometry tests showed that oryzalin concenÂtration and immersion duration directly on the vegetative material did not produce polyploid stevia individuals
Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment
Get PDF Stevia rebaudiana Bert. (Stevia) is used in the commercial and health industries because of its steviol glycosides (stevioside, reÂbaudioside A, rebaudioside C) and flavonoids. Polyploidy induction of stevia plants using oryzalin was held to increase the diversity and produce superior varieties. This research aims to obtain stevia diverÂsity with different characteristics from its diploid. The TawangmanÂgu variety of stevia seedlings was experimentally designed using a completely random design. Nine combinations of oryzalin concentraÂtions (1.5, 2.5, and 3.5μM) and immersion times (4, 6, and 8 hours) treatments were applied to 15 stevia seedlings each. Stevia without treatment was used as a control. Treatments were applied directly to the shoots on the second internode from the tip. Observations on tarÂget shoots included the number of survivors, morphological variables (height, number of internodes, internode length, leaf size, leaf thickÂness, stem diameter, and leaf color), stomata, and plant ploidy level. Data analysis on the number of live plants used frequency and deÂscriptively. Morphological data consisting of plant height, number of shoots, number of internodes, internode length, leaf length, leaf width, leaf thickness, and stem diameter were analyzed using boxplot graphs and descriptive to describe the diversity of M1 stevia treatment reÂsults. Leaf color and stomata were analyzed descriptively. The results showed that up to 3.5μM concentrations of oryzalin and 6 hours of immersion time were safe to use as a mutation agent with above 67% survival rate. Various oryzalin treatments of Tawangmangu stevia vaÂrieties yielded polyploidy morphological growth indications in height, number of internodes, internode length, stem diameter, leaf size, leaf thickness, leaf color, stomata, and stem diameter. In addition, there are growth variations such as chimeras, rosettes, and leaf splitting. However, further flow cytometry tests showed that oryzalin concenÂtration and immersion duration directly on the vegetative material did not produce polyploid stevia individuals
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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