4,772 research outputs found
Lipoprotein lipase expression in chronic lymphocytic leukemia: new insights into leukemic progression
No full textLipoprotein lipase (LPL) is a central enzyme in lipid metabolism. Due to its catalytic activity, LPL is involved in metabolic pathways exploited by various solid and hematologic malignancies to provide an extra energy source to the tumor cell. We and others described a link between the expression of LPL in the tumor cell and a poor clinical outcome of patients suffering Chronic Lymphocytic Leukemia (CLL). This leukemia is characterized by a slow accumulation of mainly quiescent clonal CD5 positive B cells that infiltrates secondary lymphoid organs, bone marrow and peripheral blood. Despite LPL being found to be a reliable molecular marker for CLL prognosis, its functional role and the molecular mechanisms regulating its expression are still matter of debate. Herein we address some of these questions reviewing the current state of the art of LPL research in CLL and providing some insights into where currently unexplored questions may lead t
Iglesia de San Pablo del Monte: Tlaxcala
No full textLa construcción del templo de San Pablo del Monte se remonta al siglo XVI. En un primer momento estuvo dedicado a San Miguel Arcángel, y poco tiempo después fue brindado al apóstol San Pablo. El templo fungió como visita del convento franciscano de Puebla, junto con las poblaciones de San Miguel Tenancingo, San Francisco Papalotla y San Cosme Mazatecochco.</p
Palabra de Pablo: 100 años después
No full text[ES] Documento que constituye una selección de textos sobre Pablo de la Torriente Brau, con ocasión del centenario de su nacimiento, recopilados por Víctor Casaus. En ella, se recorre, además, la vida del autor a través de los textos que le dedicaran los escritores cubanos desde su fallecimiento hasta nuestros días.[EN] Document that constitutes a selection of texts on Pablo de la Torriente Brau, on the occasion of the centenary of his birth, compiled by Víctor Casaus. In it, the life of the author is crossed, in addition, across the texts that him the Cuban writers were dedicating from his death to the present day
Chronic Lymphocytic Leukemia
No full textB-cell chronic lymphocytic leukemia (CLL) is considered a single disease with extremely variable course, and survival rates ranging from months to decades. It is clear that clinical heterogeneity reflects biologic diversity with at least two major subtypes in terms of cellular proliferation, clinical aggressiveness and prognosis. As CLL progresses, abnormal hematopoiesis results in pancitopenia and decreased immunoglobulin production, followed by nonspecific symptoms such as fatigue or malaise. A cure is usually not possible, and delayed treatment (until symptoms develop) is aimed at lengthening life and decreasing symptoms. Researchers are playing a lead role in investigating CLL's cause and the role of genetics in the pathogenesis of this disorder. Research programs are dedicated towards understanding the basic mechanisms underlying CLL with the hope of improving treatment options
Neoliberalismo: críticas y resistencias. Entrevista a Juan Pablo Rodríguez
No full textLa siguiente entrevista aborda algunas de las principales motivaciones, ideas y conclusiones que dan forma a la investigación contenida en el libro Resisting neoliberal capitalism in Chile: the possibility of social critique (2020) de Juan Pablo Rodríguez. Se aborda, en primer lugar, su examen de distintas teorías críticas del neoliberalismo contemporáneo; a continuación, su análisis de las prácticas de crítica situada que llevan a cabo los movimientos sociales en Chile, en especial a partir de los casos del movimiento estudiantil y el movimiento de pobladores.
----
In this interview with Juan Pablo Rodríguez, author of Resisting neoliberal capitalism in Chile: the possibility of social critique (2020), we address the main ideas and motivations behind the book and the research on which the book is based. We first explore the analysis the author makes of different theoretical critiques of contemporary neoliberal capitalism, and then we talk about the practices of embodied critiques carried out by social movements in Chile, focusing on the cases of the student and the pobladores movements
Multi-compartment and multi-host vector suite for recombinant protein expression and purification
No full textRecombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for biochemical and structural analyses. A lot of progress has been achieved in the last decades, where challenging proteins were expressed in a soluble manner after evaluating different parameters such as host, strain, and fusion partner or promoter strength, among others. In this regard, we have previously developed a vector suite that allows the evaluation of different promoters and solubility enhancer-proteins, through an easy and efficient cloning strategy. Nonetheless, the proper expression of many targets remains elusive, requiring, for example, the addition of complex post-translation modifications and/or passage through specialized compartments. In order to overcome the limitations found when working with a single subcellular localization and a single host type, we herein expanded our previously developed vector suite to include the evaluation of recombinant protein expression in different cell compartments and cell hosts. In addition, these vectors also allow the assessment of alternative purification strategies for the improvement of target protein yields
Pablo Parellada, fustigador del Modernismo
No full textabstract: ABSTRACT This research aims to investigate the work and impact of the prolific and popular Pablo Parellada y Molas. Although the author is now forgotten, he is an important figure regarding the struggles between Modernist writers and their critics. Pablo Parellada was one the key detractors of literary Modernism, a movement which he attacked through his parodies as evinced in his poetry, drama and short stories. His works contain the main pejorative features that would become the standard critique of these young poets of Spain in the early 1900's. Through the work of Pablo Parellada, this study seeks to understand the literary debates of Modernism in Spain that took place in the early twentieth century. Through this understanding, this study becomes important for it reveals the many personal characteristics that were attributed to the young Modernist poets. Some of these characteristics continued to be used by literary critics and as well became part of the collective imagination at the time and were still circulating several decades later.Dissertation/ThesisM.A. Spanish 201
Fotografía de Pablo Bielli
No full text“Innominate, unnamed, autonomous entities wondering around. From the deepest silence I find lost, starving gazes; biting looks, others that are absent. Anonymity... Some looks scream for others, even for mine... The characters that appear in this gallery of faces have caught me in some way; some are friends, or were. I have never met some of the others. However, they are all a part of me. This collection of intervened portraits is a humble homage to all anonymous beings in their purest essence. In this way, Pablo Bielli (Montevideo, 1969) introduces this series of photographs for dixit. This selection is part of the “Innominate” project, which he began in 2010, and continues developing it in different stages called albums. The author started his career in photography in 1987. He worked for several national and international media --diario El Obervador, Revista Posdata, Diario Clarín, América Economía, Editorial AVER, Revista Bla, Revista Cava Privada. Since 1998 he is a freelance photographer in the fields of advertising, editorials and international news agencies. For six years, he gave Lighting Workshop lessons at Foto Club Uruguayo. He was a founding member of Simbad Foto producciones (advertising photography) and of Fototeca Sur (archive and contents).“Sin nómina, innominados, entes autónomos deambulando. Desde el silencio más profundo encuentro miradas perdidas, hambrientas; miradas que cortan, otras ausentes. Anonimato... algunas miradas llaman a gritos a otras, incluso a la mía”. [...] “Los personajes que aparecen en esta galería de rostros me atraparon de alguna manera; algunos fueron o son amigos. A otros jamás los conocí. Y sin embrago todos son parte de mí. Este conjunto de retratos intervenidos es un humilde homenaje a todos los seres anónimos en su más pura esencia”. Así presenta Pablo Bielli (Montevideo, 1969) esta serie de fotografías para dixit. La selección forma parte del proyecto anónimos, que comenzó en 2010 y lo viene desarrollando en diferentes etapas llamadas álbumes. El autor se inició en la fotografía en 1987. Trabajó para varios medios nacionales e internacionales –diario El Observador, revista Posdata, diario Clarín, América Economía, editorial AVER, revista Bla, revista Cava Privada). Desde 1998 se desempeña como fotógrafo independiente para publicidad, editoriales y agencias internacionales de noticias. Durante seis años ejerció la docencia en Iluminación en el Foto Club Uruguayo. Fue socio fundador de Simbad Foto producciones (fotografía publicitaria) y de Fototeca Sur (archivo y contenidos). [email protected]
Fotografía de Pablo Bielli
No full text“Innominate, unnamed, autonomous entities wondering around. From the deepest silence I find lost, starving gazes; biting looks, others that are absent. Anonymity... Some looks scream for others, even for mine... The characters that appear in this gallery of faces have caught me in some way; some are friends, or were. I have never met some of the others. However, they are all a part of me. This collection of intervened portraits is a humble homage to all anonymous beings in their purest essence. In this way, Pablo Bielli (Montevideo, 1969) introduces this series of photographs for dixit. This selection is part of the “Innominate” project, which he began in 2010, and continues developing it in different stages called albums. The author started his career in photography in 1987. He worked for several national and international media --diario El Obervador, Revista Posdata, Diario Clarín, América Economía, Editorial AVER, Revista Bla, Revista Cava Privada. Since 1998 he is a freelance photographer in the fields of advertising, editorials and international news agencies. For six years, he gave Lighting Workshop lessons at Foto Club Uruguayo. He was a founding member of Simbad Foto producciones (advertising photography) and of Fototeca Sur (archive and contents).“Sin nómina, innominados, entes autónomos deambulando. Desde el silencio más profundo encuentro miradas perdidas, hambrientas; miradas que cortan, otras ausentes. Anonimato... algunas miradas llaman a gritos a otras, incluso a la mía”. [...] “Los personajes que aparecen en esta galería de rostros me atraparon de alguna manera; algunos fueron o son amigos. A otros jamás los conocí. Y sin embrago todos son parte de mí. Este conjunto de retratos intervenidos es un humilde homenaje a todos los seres anónimos en su más pura esencia”. Así presenta Pablo Bielli (Montevideo, 1969) esta serie de fotografías para dixit. La selección forma parte del proyecto anónimos, que comenzó en 2010 y lo viene desarrollando en diferentes etapas llamadas álbumes. El autor se inició en la fotografía en 1987. Trabajó para varios medios nacionales e internacionales –diario El Observador, revista Posdata, diario Clarín, América Economía, editorial AVER, revista Bla, revista Cava Privada). Desde 1998 se desempeña como fotógrafo independiente para publicidad, editoriales y agencias internacionales de noticias. Durante seis años ejerció la docencia en Iluminación en el Foto Club Uruguayo. Fue socio fundador de Simbad Foto producciones (fotografía publicitaria) y de Fototeca Sur (archivo y contenidos). [email protected]
Ibrutinib therapy downregulates AID enzyme and proliferative fractions in chronic lymphocytic leukemia
No full textActivation-induced cytidine deaminase (AID) initiates somatic hypermutation and class switch recombination of the immunoglobulin genes. As a trade-off for its physiological function, AID also contributes to tumor development through its mutagenic activity. In chronic lymphocytic leukemia (CLL), AID is overexpressed in the proliferative fractions (PFs) of the malignant B lymphocytes, and its anomalous expression has been associated with a clinical poor outcome. Recent preclinical data suggested that ibrutinib and idelalisib, 2 clinically approved kinase inhibitors, increase AID expression and genomic instability in normal and neoplastic B cells. These results raise concerns about a potential mutagenic risk in patients receiving long-term therapy. To corroborate these findings in the clinical setting, we analyzed AID expression and PFs in a CLL cohort before and during ibrutinib treatment. We found that ibrutinib decreases the CLL PFs and, interestingly, also reduces AID expression, which correlates with dampened AKT and Janus Kinase 1 signaling. Moreover, although ibrutinib increases AID expression in a CLL cell line, it is unable to do so in primary CLL samples. Our results uncover a differential response to ibrutinib between cell lines and the CLL clone and imply that ibrutinib could differ from idelalisib in their potential to induce AID in treated patients. Possible reasons for the discrepancy between preclinical and clinical findings, and their effect on treatment safety, are discussed.Fil: Morande, Pablo Elías. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sivina, Mariela. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Uriepero, Angimar. Instituto Pasteur de Montevideo; UruguayFil: Seija, Noé. Instituto Pasteur de Montevideo; UruguayFil: Berca, Catalina. Instituto Pasteur de Montevideo; UruguayFil: Fresia, Pablo. Instituto Pasteur de Montevideo; UruguayFil: Landoni, Ana Inés. Ministerio de Salud.Hospital Maciel. Administración de los Servicios de Salud del Estado; UruguayFil: Di Noia, Javier M.. University of Montreal; Canadá. Cliniques de Montreal. Institut de Recherches. Division of Immunity and Viral Infections; CanadáFil: Burger, Jan A.. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Oppezzo, Pablo. Instituto Pasteur de Montevideo; Urugua
- …
