3,144 research outputs found
On the Optimal Operation Frequency to Minimize Phase Noise in Integrated Harmonic Oscillators
No full textThis brief aims at finding the most appropriate frequency of operation of an integrated harmonic oscillator in order to maximize its spectral purity. To achieve this, a simple, yet accurate, scalable model is developed for the LC tank, that tracks the dependence of the parasitics on the inductance value. Using an ultra-scaled CMOS digital technology as a case study, the frequencies around 5 GHz are singled out as the sweet spot to minimize the phase noise
PRaTo: A web-tool to select optimal primer pairs for qPCR
No full textAn essential pre-requisite to perform sound quantitative real-time polymerase chain reaction (qPCR) assays is to design outstanding primer pairs. This means they must have a good efficiency and be not prone to produce multiple amplicons or primer dimer products. To circumvent these issues, several softwares are available to help primer design. Although satisfactory computer-aided primer design tools are available for standard PCR, less efforts were done to provide specific methods for selection of optimal primer pairs for qPCR. We have developed PRaTo a web-based tool that enables checking and ranking of primers pairs for their attitude to perform optimally and reliably when used in qPCR experiments. PRaTo is available at http://prato.daapv.unipd.it
A imagem de Alessandro Baricco no Brasil
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-Graduação em Estudos da Tradução, Florianópolis, 2013.Com a intenção de delinear o modo pelo qual o escritor italiano Alessandro Baricco se inseriu no sistema literário brasileiro e os caminhos percorridos pelos seus livros traduzidos, esta dissertação dá voz às experiências tradutórias de seus tradutores. A inserção de Bariccono Brasil tem seu início em 1997, através de uma proposição da Profa. Dra. Roberta Barni à editora Iluminuras da tradução de Oceano Mare. A partir daí, outras sete obras foram publicadas no Brasil, sendo três delas traduzidas por Roberta Barni e as outras quatro por quatro tradutores diferentes. De um lado, considera-se o tradutor como figura principal namediação entre culturas, e, de outro, se analisa a realidade desta figuradentro do sistema literário, sua invisibilidade, seus limites e o exercíciode sua profissão. A pesquisa conta, ainda, com críticas e resenhas referentes ao autor italiano publicadas em jornais consagrados no Brasil, considerando estas como parte constituinte da imagem de Baricco refletida em território nacional. Abstract : Intending to delineate the way the Italian writer Alessandro Baricco has been inserted in the Brazilian literary system and the paths his translated books have followed, this thesis gives voice to the translating experiences of his translators. Baricco's insertion in Brazil began in 1997, through a personal project of Dr. Roberta Barni, with her translation of Oceano Mare. Since then, seven other of his works have been published in Brazil, three of which were translated by Roberta Barni and the other four by four different translators. On the one hand,the translator is considered as the main figure in mediation betweencultures and, on the other, this figure's reality is analyzed within theliterary system: its invisibility, its limits and its professional practice. Criticisms and reviews of this Italian author published in well established Brazilian newspapers are also considered, with the understanding that they are part of Baricco's image reflected here
Evaluation of candidate reference genes for qPCR in maize
No full textQuantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels. Accurate and
reproducible results are dependent on the correct choice of the reference genes for data normalization.
To date, screenings evaluating candidate reference gene stability for expression studies in maize have
not been reported. In the present work, we analyzed the expression patterns of 12 genes in a set of 20
maize samples, obtained from different tissues of plants grown at various experimental conditions. Using
genormPLUS, NormFinder and BestKeeper algorithms, the expression stability of three “classical” reference
genes, such as ACT, TUB and 18S rRNA, and the newly identified candidates, was assessed. With respect to
the algorithms, our results showed similar performance among genormPLUS, NormFinder and BestKeeper
in evaluating the suitability of reference genes. Our data therefore showed that the currently and widely
used reference genes for data normalization in maize were not the most stable expressed transcripts. Five
of the new putative reference genes (CUL, FPGS, LUG, MEP and UBCP) exhibited the highest expression
stability according to all algorithms. In conclusion, with this study, we provide a list of validated reference
genes and their relative primer sequences to conduct reliable qPCR experiments in maize
La maturità di Alessandro Fei del Barbiere, in bilico tra Maniera e Riforma
No full textThis article studies the mature career of the Florentine painter Alessandro Fei del Barbiere (1537-1592), beginning with the rediscovery of the 'Ascension' altarpiece formerly in the Albizi Chapel in the destroyed church of San Pier Maggiore, Florence. Studying this painting and others recorded in 1584 by the biographer Raffaello Borghini, such as the two altarpieces for Santa Maria delle Grazie and the Madonna dell'Umiltà in Pistoia, the author reconstructs a body of works showing how in the 1580s Fei gradually went beyond the archaic style of his apprenticeship - he had been trained by Ridolfo del Ghirlandaio and Pierfrancesco Foschi, but was also marked by the Maniera of Vasari - evolving towards naturalism in both mimesis and pictorial handling. In Florence, his development partly parallels that of Santi di Tito and his circle, but Fei was also influenced by a probable sojourn during the early part of that decade in Rome, where he could have been inspired by Girolamo Muziano and the painters working for Pope Gregory XIII. Among other proposals, the author suggests that the artist was responsible for decorating the chancel of Fiesole Cathedral (c. 1584-1589), which consisted of an altarpiece, only rarely discussed by scholars, and a cycle of frescoes hitherto attributed to Nicodemo Ferrucci
Tra socialdemocrazie e Perestrojka. Le relazioni internazionali del Pci attraverso le carte di Alessandro Natta
No full textThis essay reconstructs the foreign policy of the Italian Communist Party during the four
years of Alessandro Natta’s secretariat (1984-1988) through largely original archival documentation,
from the Alessandro Natta Fonds of the Historical Archive of the Chamber of
Deputies. Natta’s papers are also cross-referenced with those kept in the PCI Archive at the
Gramsci Foundation. The author analyses the relationship that the PCI establishes with
Gorbachev’s Perestroika, Deng’s China, and European social democracies during the last
years of the Cold War
A 19.5 GHz 28 nm CMOS class-C VCO with reduced 1/f noise upconversion
No full textClass-C operation is leveraged to implement a K-band CMOS VCO where the upconversion of the 1/f noise from the core transistors is robustly contained at a minimal level. Implemented in a bulk 28 nm CMOS technology,the VCO shows a phase noise as low as -108.5 dBc/Hz at 1 MHz offset (-83 dBc/Hz at 100 kHz offset) from the 19.5 GHz carrier,while consuming 14.4 mW and featuring a 12% tuning range
Volta Alessandro
No full textThe New Dictionary of Scientific Biography (extension and updating of the Dictionary of Scientific Biography) is one of the most substantial reference works in the field of the history of science. An up-to-date overview on Alessandro Volta's science with new perspectives offered by the author. Information and critical analysis is also provided on the main secondary literature produced on this main protagonist of enlightenment science after the 1976 entry "Volta Alessandro" in the Dictionary of Scientific Biography
Effect of the number of removed lymph nodes on prostate cancer recurrence and survival: evidence from an observational study
No full textBackground: The aim of this article is to analyze the effect on biochemical recurrence and on overall survival of removing an extensive number of pelvic lymph nodes during prostate cancer surgery. The lack of evidence from randomized clinical trials to address this specific question has hampered the ability to determine the true effect of the number of nodes removed.Results: Our analysis is based on a large observational study, and this can lead unadjusted estimates to be very sensitive to confounding bias due to the different prognosis of individuals. We assess the effect of the number of lymph nodes removed by means of an Inverse Probability Weighting adjustment based on a Poisson regression model, and by a Doubly-robust adjustment.Conclusions: Our findings suggest that a large number of nodes removed is associated with a significant improvement in time to biochemical recurrence. However, it appears to have no impact on overall survival
Genome-wide discovery and characterization of nitrate-responsive miRNAs in roots of maize seedlings
No full textAbstract
Nitrogen availability affects crops productivity and environment. The natural abundance of useable nitrogen is so low that the massive human alteration of the nitrogen cycle has been required to sustain the feeding of the world's population. Tons of nitrogenous fertilizers are added to the soil worldwide annually, giving rise to environmental diseases. In this scenario, the knowledge of post-transcriptional regulation of plant response to nutrients is important to improve nitrogen use efficiency of crop. With the identification of stress-responsive miRNAs, a layer of post-transcriptional gene regulation has been uncovered.
We used a maize miRNAs-microarray platform to discover previously unknown nitrate-responsive miRNAs. Six mature miRNAs were identified and their expression profiles were studied by quantitative Real Time PCR (qPCR) and in situ hybridization (ISH) in maize roots grown in different nitrate availabilities.
Significant differences in miRNAs’ transcripts accumulation were evidenced between nitrate-supplied and nitrate-depleted roots. Real time PCR analyses and in situ detection of miRNAs confirmed the arrays data and evidenced distinct miRNAs spatiotemporal expression patterns in maize roots.
An in silico approach was used to select target genes of the miRNAs identified. Their transcripts accumulation has been investigated in both nitrate-supplied and nitrate-depleted roots by means of qPCR and ISH.
Our results suggest that miRNAs play some role in modulating N-responsive gene expression by inducing post-transcriptionally the expression of target genes. In particular, the repression of the transcription of miRNA identified upon nitrate shortage could represent a crucial step integrating nitrate signals into developmental changes in maize roots
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