73 research outputs found
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Yamamba's Amorphous Self and the Marginal Space in Ohba Minako's Stories
No full textThis thesis deals with how Ohba Minako, a Japanese woman author who was prolific in the late twentieth century, uses a Japanese female yōkai (or “supernatural monster”) called yamamba (often translated into “mountain witch”) in order to produce a non-hierarchical community in her short stories and novels. Yamamba are usually depicted as old women who lure lost male travelers in the mountains into their huts in order to eat them. Therefore, feminist scholars analyze this figure from a feminist perspective as a reflection of misogyny in the patriarchal society. Acknowledging the usefulness and validity of the feminist approach and expanding it into viewing vagabonds and immigrants’ marginal communities, I will focus on how Ohba emphasizes the yamamba’s amorphous self, which I will explain constantly changes and thus carries the potential to transcend the border between the self and the other. Ohba’s depictions of yamamba as a mind-reader and women who speak with a language that does not belong to any specific nations or races are, I will argue, all part of her efforts to highlight the vi social injustices of putting individuals into certain molds of identities and her declarations to oppose to them as a woman and as a foreigner who lived in immigrants’ communities.Master of Arts (M.A.
Adventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba
No full textAdventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba
No full textAdventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba
No full textDue to indiscriminate collection, the natural habitat of Hylotelephium sieboldii and H. sieboldii var. ettyuense have been significantly reduced. For ex situ conservation and efficient vegetative propagation, a micropropagation system based on adventitious shoot regeneration was developed for these two endangered species. Leaves, stems and roots of in vitro-grown plantlets, and flower buds of greenhouse-grown plants were used as explants. For H. sieboldii, adventitious shoots were most efficiently regenerated from flower bud explants on a medium containing 1 mg l−1 each of NAA and BA. Adventitious shoot regeneration from flower bud explants under this condition was also obtained in H. sieboldii var. ettyuense, but with lower efficiencies. Adventitious shoots of both species rooted and developed into plantlets on a medium containing 0.1 mg l−1 IBA. Almost all of these plantlets were successfully transplanted to the greenhouse. At least at early stage of growth, they showed no apparent morphological alterations
Budding of Ebola Virus Particles Requires the Rab11-Dependent Endocytic Recycling Pathway
No full textThe Ebola virus-encoded major matrix protein VP40 traffics to the plasma membrane, which leads to the formation of filamentous viral particles and subsequent viral egress. However, the cellular machineries underlying this process are not fully understood. In the present study, we have assessed the role of host endocytic recycling in Ebola virus particle formation. We found that a small GTPase Rab11, which regulates recycling of molecules among the trans-Golgi network, recycling endosomes, and the plasma membrane, was incorporated in Ebola virus-like particles. Although Rab11 predominantly localized in the perinuclear region, it distributed diffusely in the cytoplasm and partly localized in the periphery of the cells transiently expressing VP40. In contrast, Rab11 exhibited a perinuclear distribution when 2 VP40 derivatives that lack ability to traffic to the plasma membrane were expressed. Finally, expression of a dominant-negative form of Rab11 or knockdown of Rab11 inhibited both VP40-induced clusters at the plasma membrane and release of viral-like particles. Taken together, our findings demonstrate that Ebola virus exploits host endocytic recycling machinery to facilitate the trafficking of VP40 to the cell surface and the subsequent release of viral-like particles for its establishment of efficient viral egress
Physico-chemical Properties and Digestibility of Pulse Starch after Four Different Treatments
No full textOS PÉS DESCALÇOS DE GEN – HISTÓRIA, MEMÓRIA
No full textTCC (graduação) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Departamento de História.Este trabalho tem como objetivo analisar a obra de Keiji Nakazawa, Gen Pés Descalços em seus dois primeiros volumes, e problematizar a obra dentro da análise das histórias em quadrinhos como fonte documental e histórica, analisando-a em seus detalhes, buscando compreender as perspectivas utilizadas pelo autor para tratar da explosão atômica de Hiroshima.This work aims to analyze the work of Keiji Nakazawa , Barefoot Gen in his first two volumes , and discuss the work in the analysis of comics as documentary and historical sources , analyzing it in detail , trying to understand the perspectives used by the author to discuss the atomic explosion in Hiroshima
Visualization of Ras-PI3K interaction in the endosome using BiFC
No full textRecent studies indicate the importance of spatiotemporal regulation in the diversity and specificity of intracellular signaling. Here, we show that Ras-PI3K signaling plays an important role in the local regulation of phosphatidylinositol metabolism in the endosome through live-cell imaging by using a bimolecular fluorescence complementation technique, in which molecular interaction is indicated by fluorescence emission. Using several possible combinations of Ras and the Ras-binding domain, we identified an optimal set of probe molecules that yielded the most significant increase in fluorescence intensity between the active and inactive forms of Ras. This combination revealed that, among the Ras effectors tested, phosphatidylinositol 3-kinase (PI3K) was specifically implicated in signaling in the endosome. We also found that full length PI3K was recruited to the endosome in EGF- and Ras-dependent manners, which appears to be essential for the activation of PI3K in this compartment. Taken together, these findings demonstrate that the spatiotemporal regulation of Ras-PI3K signaling may dictate the activation of PI3K and subsequent downstream signaling in the endosome
Role of endocytic pathway in cell-to-cell EBV transmission
No full textEpstein-Barr virus (EBV) establishes a lifelong latent infection in B lymphocytes and often is found in epithelial cells. Several lines of evidence indicate that viral transmission mediated by cell-to-cell contact is the dominant mode of infection by EBV for epithelial cells. However, its detailed molecular mechanism has not been fully elucidated. We investigated the role of host membrane trafficking machinery in this process. We have found that adhesion molecules critical for this process are expressed in EBV-positive and -negative Burkitt's lymphoma (BL) cells and multiple epithelial cell lines. Treatment with blocking antibodies against β1 and β2 integrin families and their ligands suppressed EBV transmission in a dose-dependent manner. We also confirmed that adhesion molecules are upregulated in co-cultured BL cells. Immunofluorescence staining revealed that the intracellular adhesion molecule 1 (ICAM-1) distributed to the cell surface and partially co-localized with recycling endosomes in co-cultured BL cells. Moreover, cell-to-cell EBV transmission was inhibited upon blocking endocytic recycling by expression of a dominant-negative form of a small GTPase Rab11 or by knockdown of Rab11, supporting the notion that the endocytic pathway-dependent trafficking of ICAM-1 to the cell surface of BL cells contributes to viral transmission by stabilizing cell-to-cell contact between the donor cells and recipient cells. Finally, we demonstrated that co-cultivation upregulated clathrin-mediated endocytosis in the recipient cells, allowing EBV to be internalized. Taken together, our findings demonstrate that EBV exploits host endocytic machinery in both donor and recipient cells, a process which is facilitated by cell-to-cell contact, thereby promoting successful viral transmission
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