286 research outputs found
Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes
No full textA patent application describing the clonogrouping method was submitted (V. Chenal-Francisque, M. M. Maury, M. Lavina, M. Touchon, A. Leclercq, M. Lecuit, and S. Brisse, patent application EP 15 306 154.4, 10 July 2015, European Patent Office).International audienceThree multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones
Draft Genome Sequence of Campylobacter coli Strain IPSID-1 Isolated from a Patient with Immunoproliferative Small Intestinal Disease
No full textInternational audienceThe genome sequence and annotation of Campylobacter coli strain IPSID-1 are reported here. This bacterial isolate is the first to be cultured from a patient with immunoproliferative small intestinal disease (IPSID). The draft genome sequence is 1.683 Mb long, comprises 64 contigs, and has 31.26% G؉C content. Citation Criscuolo A, de la Blanchardière A, Coeuret S, Passet V, Saguet-Rysanek V, Vergnaud M, Verdon R, Leclercq A, Lecuit M, Brisse S. 2014. Draft genome sequence of Campylobacter coli strain IPSID-1 isolated from a patient with immunoproliferative small intestinal disease
Confocal Microscopy Imaging of Peptidoglycan Uptake in the Mouse Intestine
No full textConfocal imaging data set accompanying Fig.5 and Fig.6 for the article: Wheeler R, Dias Bastos PA, Disson O, Rifflet A, Gabanyi I, Spielbauer J, Bérard M, Lecuit M, Gomperts Boneca I. Microbiota-induced active translocation of peptidoglycan across the intestinal barrier dictates its within-host dissemination (2023) PNAS; doi: 10.1073/pnas.2209936120
Stainings are described in detail in the the PNAS article methodology.
Files names beginning Fig 5A-1; DAPI, WGA, anti-E-cadherin, MDP-rhodamine
Files names beginning Fig 5A-2, Fig 5A-3 & Fig S5; DAPI, Phalloidin, anti-Siglec-F, MDP-rhodamine
Files names beginning Fig 5B; DAPI, Phalloidin, E.coli peptidoglycan-Alexa Fluor 647 conjugate
Files names beginning Fig 6B; DAPI, Phalloidin, anti-Siglec-F, MDP-rhodamine (also corresponds to Supplementary data Figure 5)
Files names beginning Fig S5-1; DAPI, Phalloidin, anti-CgA, MDP-rhodamine
Files names beginning Fig S5-2; DAPI, Phalloidin, anti-NKM-16-4-2, MDP-rhodamineFiles may be opened using open source software such as imageJ
The Dynamics of Living Systems
No full textMr Administrator,My dear colleagues,My dear friends,Ladies and gentlemen, What is life? Though life appears self-evident, it actually remains quite elusive. Is it a substance, a structure, a process? For Henri Bergson, “life is evolution”; “the organism changes at every moment. That which is real is the continuous evolution of form: form is only an instantaneous snapshot of a transition”. These words highlight a central property of living systems, namely an internal power of transformation, m..
InlA<sup>m</sup> mediates targeting of villous M cells.
No full text<p>The intestinal ileum was taken from E16P KI mice and wt mice orally inoculated by 10<sup>10 </sup><i>Li-inlA</i> and <i>Li-inlA<sup>m</sup></i> at 5 hr post infection, respectively. The intestinal tissues were fixed and stained with WGA for goblet cells, NKM 16-2-4 monoclonal antibody for M cells, and for bacteria and nuclei. (A and B) The confocal Z-plane of an ileal villus from <i>Li-inlA<sup>m</sup></i> infected wt mice demonstrates that <i>Li-inlA<sup>m</sup></i> was able to target goblet cells (A) and villous M cells (B). Right panels show separated channels and merge of boxed regions, showing the location of bacteria in villous epithelia. (C) The confocal Z-plane of an ileal villus from <i>Li-inlA</i> infected E16P KI mice shows that <i>Li-inlA</i> targeted goblet cells. (D) Relative location of bacteria in mice intestinal epithelia of villi is shown. The total number of <i>Li-inlA<sup>m</sup></i> in wt mice intestinal villi epithelia was set to 100. 20 villi from two mice ileal loops were counted in each set. Scale bar, 20 µm. See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s004" target="_blank"> Figures S4</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s005" target="_blank">S5</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s006" target="_blank">S6</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s011" target="_blank">Movies S4</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s012" target="_blank">S5</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s013" target="_blank">S6</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003381#ppat.1003381.s014" target="_blank">S7</a>.</p
Reply to the article 'Hepatitis C virus (HCV) infection and MALT-type ocular adnexal lymphoma (OAL)' by P. Arnaud, M.-C. Escande, M. Lecuit et al. [6]
No full textThe role of HCV in lymphomagenesis is indicated by the higher prevalence of HCV seropositivity in patients with B-cel
Lessons learnt from the emergence of Zika virus
No full textpeer reviewedLooking back at how Zika virus emergence was handled during and after the 2015–2016 outbreak will be important for assessing how well multiple relevant stakeholders were integrated to mount a response, and can provide the groundwork to better cope with emerging infections in the future. © 2018, The Author(s)
Le lien paradoxal entre connaissance de soi et relation à Dieu. Actualité du « socratisme chrétien »
No full textInternational audienceDespite appearances, progress in self-knowledge does not contradict the deepening of the relationship with God, but favors it. After having shown this, the author exposes the nature and conditions of self-knowledge according to the "Christian Socratism" of the authors who, from Origen to Pascal, have made theirs the famous precept: "know thyself". Particular attention is paid to the teaching of Teresa of Avila and John of the Cross on the paradoxical link between self-knowledge and relationship to God. In the third and longer part of the article, the author evokes the experience of this link, considering successively the knowledge of self as marked by evil, the knowledge of the other in oneself (God, or the neuro-psychic unconscious?), and the knowledge of the good in oneself, source of knowledge of God: in the light of faith, the experience of self as living, believing, and loved is transformed into knowledge of God as interior, loving, desiring, and source of love.Malgré les apparences, le progrès dans la connaissance de soi ne contredit pas l'approfondissement de la relation à Dieu, mais le favorise. Après avoir montré cela, l'auteur expose la nature et les conditions de la connaissance de soi selon le « socratisme chrétien » des auteurs qui, d'Origène à Pascal, ont fait leur le fameux précepte : « connais-toi toi-même ». Une attention particulière est portée à l'enseignement de Thérèse d'Avila et Jean de la Croix sur le lien paradoxal entre connaissance de soi et relation à Dieu. Dans la troisième et plus longue partie, l'auteur évoque l'expérience de ce lien, en considérant successivement la connaissance de soi comme marqué par le mal, la connaissance de l'autre en soi (Dieu, ou l'inconscient neuro-psychique ?), et la connaissance du bien en soi, source de connaissance de Dieu : à la lumière de la foi, l'expérience de soi comme vivant, croyant, et aimé se transforme en connaissance de Dieu comme intérieur, aimant, désirant, et source d'amour
Zeta potential in ceramic industry
No full textDeflocculation, electrical conductivity and zeta potential (ZP) are studied for the addition of 0 to 10000 ppm Na2SiO3 deflocculator to slips obtained from three argillaceous materials (kaolin d'Arvor, ball clay Hyplas 64, and/or Granger Clay No. 10). The quantity of Na2SO3 required to deflocculate a slip is independent of the density but differes for each clay. The ZP is directly related to the density of the slip. The higher the ZP the more stable a slip is; the value of the ZP of a mixture does not follow a simple law but the electrical resistance of a mixture does follow a simple additive law. The ZP appears to have linear relation with the specific surface of the argillaceous material
Understanding how Listeria monocytogenes targets and crosses host barriers
No full textABSTRACTHuman listeriosis is caused by the Gram-positive bacterium Listeria monocytogenes. In humans, this pathogen has the ability to cross the intestinal, placental and blood–brain barriers, leading to gastroenteritis, maternofetal infections and meningoencephalitis, respectively. The entry of L. monocytogenes into cultured human epithelial cells is mediated by the interaction of an L. monocytogenes surface protein, internalin, with its human receptor, E-cadherin. The internalin–E-cadherin interaction is species-specific, and relies on the nature of a single amino-acid in the E-cadherin molecule, which is proline in permissive species such as humans, and glutamic acid in non-permissive species such as the mouse. In a transgenic mouse model that expresses human E-cadherin in enterocytes, internalin allows L. monocytogenes to cross the intestinal barrier. Epidemiological evidence also supports a role for internalin in human listeriosis, not only for crossing the intestinal barrier, but also for targeting and crossing the placental and blood–brain barriers. Consistent with these epidemiological data, infection with L. monocytogenes of trophoblastic cell lines, primary trophoblast cultures and human placental villous explants demonstrates that bacterial invasion of the syncytiotrophoblast barrier is mediated by the internalin–E-cadherin interaction, leading to histopathological lesions that mimic those seen in the placentas of women with listeriosis. Thus, the internalin–E-cadherin interaction that plays a key role in the crossing of the intestinal barrier in humans is also exploited by L. monocytogenes to target and cross the placental barrier. Further investigations are currently focusing on the molecular mechanisms by which L. monocytogenes targets and crosses the blood–brain barrier
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