3,364 research outputs found
Erythrocyte incubation as a method for free-dye presence determination in fluorescently labeled nanoparticles
No full textThe field of nanotheranostics encompasses the integration of nanosized carriers in cancer imaging, diagnosis, and therapy. The use of nanomedicines for theranostic application typically depends on direct visualization of the nanocarriers. Normally fluorescent probes are attached to nanocarriers for biodistribution measurement through fluorescence imaging. However continued, noninvasive assurance that the fluorescent probe remains bound to the carrier has proven elusive. Mature erythrocytes, also known as red blood cells, are incapable of endocytosis. As a consequence, when incubated with fluorescently labeled particles, they do not show any signal coming from the membrane or the cytoplasm. Yet, these cells readily take up free BODIPY fluorescent dyes into their membranes. Here we show that incubation of nanoparticles with erythrocytes is a rapid and reliable method for the detection of unbound dye present within a nanoparticle sample, as the detection of a fluorescent signal coming from the cells can only be due to unbound dye present in the sample. We test the method on both sulfonate and PEG terminated gold nanoparticles, and we determine the minimum concentration of detectable dye for a specific gold nanoparticle sample. © 2012 American Chemical Society
Concluding remarks
No full textAlthough undoubtedly much work remains yet to be done to untangle the complex web of personal relationships within the extended royal oikos of Ancient Macedonia, building on the seminal scholarship of Elizabeth Carney the papers in this volume have elucidated the important role played by affective bonds. Although the king did hold the balance of power at the Argead court, the cultivation of a personal relationship offered members of his entourage (in whatever capacity) a certain degree of agency.
The power dynamic therefore was not entirely dictated by the king, and the bonds that members of the court were able to develop with him afforded a certain degree of reciprocity. The social structure of the Argead courts of Philip and Alexander was highly influential in the development of their own court systems by the Successors, who adapted and transformed it as necessary for the success of their new dynasties in the changed world of the Hellenistic kingdoms
Description prospective des manifestations du complexe de Carney : première analyse du PHRC national EVA-Carney
No full textInternational audiencePOP-015Description prospective des manifestations du complexe de Carney : première analyse du PHRC national EVA-CarneyLe complexe de Carney (CNC), souvent lié à une mutation de PRKAR1A, est une néoplasie multiple endocrine et non endocrine rare.ObjectifDescription des manifestations de la maladie à partir d’explorations prospectives standardisées.MéthodePHRC national Eva-carney avec explorations standardisées annuelles prospectives sur 3 ans de 69 patients CNC ou apparentés mutés pour PRKAR1A.RésultatsLa fréquence, la nature des anomalies observées dans la maladie et leur cinétique d’apparition sont décrites : lentigines (57 % des patients), myxome cutané (17 %), atteinte nerveuse (8,7 %), hypophysaire (15 %), thyroïdienne (20 %), testiculaire (35 %), osteochondromyxomes (3 %) (étude préliminaire). Dix-neuf pour cent des patients avaient un antécédent de myxome cardiaque. Au cours du suivi, un premier myxome et une récidive ont été diagnostiqués chez 4 % et 6 % des patients respectivement. Une dysplasie micronodulaire pigmentée des surrénales était connue pour la moitié des patients. Un quart des autres patients ont présenté une anomalie surrénalienne. Au total, 57 % des patients ont eu une surrénalectomie à la fin du suivi. La réalisation systématique d’IRM rachidienne a permis de décrire pour la première fois des anomalies nodulaires vertébrales asymptomatiques d’allure non évolutive chez 45 % des patients. Les patients porteurs de mutations exoniques de PRKAR1A avaient plus volontiers ces lésions osseuses ou un phénotype sévère que ceux porteurs de mutations introniques.ConclusionLe phénotype du CNC s’étend de formes pauci-symptomatiques à des formes sévères caractérisées par un grand nombre d’atteintes et/ou de manifestations graves. Les lésions rachidiennes décrites ici sont très probablement une nouvelle manifestation du CNC
Tear Buffering in Contact Lens Wearers
No full textThe buffering capacity of tears collected from contact lens wearers was compared to that from non-contact lens wearers. Three groups of subjects were investigated: 6 subjects not wearing contact lenses, 3 subjects wearing rigid contact lenses, and 3 subjects wearing hydrogel contact lenses. Each of the 12 subjects provided, on 6 separate occasions, 100 microliters of tears. The pH of the tears was measured with a closed chamber, temperature stabilized micro-electrode system firstly on collection and then after titration with acid and base. In all, 28 titration steps across the acid-base spectrum were completed for each subject's tears on each occasion. These tear pH responses to acid-base challenge were compared to that of unbuffered distilled water for the same titration procedure. There was no statistically significant effect of contact lens wear on tear buffering capacity, but considerable inter-subject variability was noted
Exercise as Medicine for Mental and Substance Use Disorders: A Meta-review of the Benefits for Neuropsychiatric and Cognitive Outcomes
No full textBACKGROUND: Exercise may improve neuropsychiatric and cognitive symptoms in people with mental disorders, but the totality of the evidence is unclear. We conducted a meta-review of exercise in (1) serious mental illness (schizophrenia spectrum, bipolar disorder and major depression (MDD)); (2) anxiety and stress disorders; (3) alcohol and substance use disorders; (4) eating disorders (anorexia nervosa bulimia nervosa, binge eating disorders, and (5) other mental disorders (including ADHD, pre/post-natal depression). METHODS: Systematic searches of major databases from inception until 1/10/2018 were undertaken to identify meta-analyses of randomised controlled trials (RCTs) of exercise in people with clinically diagnosed mental disorders. In the absence of available meta-analyses for a mental disorder, we identified systematic reviews of exercise interventions in people with elevated mental health symptoms that included non-RCTs. Meta-analysis quality was assessed with the AMSTAR/+. RESULTS: Overall, we identified 27 systematic reviews (including 16 meta-analyses representing 152 RCTs). Among those with MDD, we found consistent evidence (meta-analyses = 8) that exercise reduced depression in children, adults and older adults. Evidence also indicates that exercise was more effective than control conditions in reducing anxiety symptoms (meta-analyses = 3), and as an adjunctive treatment for reducing positive and negative symptoms of schizophrenia (meta-analyses = 2). Regarding neurocognitive effects, exercise improved global cognition in schizophrenia (meta-analyses = 1), children with ADHD (meta-analyses = 1), but not in MDD (meta-analyses = 1). Among those with elevated symptoms, positive mental health benefits were observed for exercise in people with pre/post-natal depression, anorexia nervosa/bulimia nervosa, binge eating disorder, post-traumatic stress disorder and alcohol use disorders/substance use disorders. Adverse events were sparsely reported. CONCLUSION: Our panoramic meta-overview suggests that exercise can be an effective adjunctive treatment for improving symptoms across a broad range of mental disorders.sponsorship: Brendon Stubbs holds a Clinical Lectureship supported by Health Education England and the NIHR Integrated Clinical Academic (ICA) Programme (ICA-CL-2017-03-001). Brendon Stubbs is also part supported by the Maudsley Charity and the National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care South London at King's College Hospital NHS Foundation Trust. The views expressed are those of the author[s] and not necessarily those of the NHS, the NIHR or the Department of Health and Social Care. John Firth is supported by a Blackmores Institute Fellowship. Rebekah Carney is funded by the Research Capability Fund via Greater Manchester West Mental Health NHS Foundation Trust. Garcia Ashdown-Franks is funded by a Mitacs Globalink Research Award. (Health Education England, NIHR Integrated Clinical Academic (ICA) Programme|ICA-CL-2017-03-001, Maudsley Charity, National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care South London at King's College Hospital NHS Foundation Trust, Blackmores Institute Fellowship, Research Capability Fund via Greater Manchester West Mental Health NHS Foundation Trust, Mitacs Globalink Research Award)status: Publishe
Estimating the parameters of globular cluster M 30 (NGC 7099) from time-series photometry
No full textAims. We present the analysis of 26 nights of V and I time-series observations from 2011 and 2012 of the globular cluster M 30 (NGC 7099). We used our data to search for variable stars in this cluster and refine the periods of known variables; we then used our variable star light curves to derive values for the cluster's parameters. Methods. We used difference image analysis to reduce our data to obtain high-precision light curves of variable stars. We then estimated the cluster parameters by performing a Fourier decomposition of the light curves of RR Lyrae stars for which a good period estimate was possible. We also derived an estimate for the age of the cluster by fitting theoretical isochrones to our colour-magnitude diagram (CMD). Results. Out of 13 stars previously catalogued as variables, we find that only 4 are bona fide variables. We detect two new RR Lyrae variables, and confirm two additional RR Lyrae candidates from the literature. We also detect four other new variables, including an eclipsing blue straggler system, and an SX Phoenicis star. This amounts to a total number of confirmed variable stars in M 30 of 12. We perform Fourier decomposition of the light curves of the RR Lyrae stars to derive cluster parameters using empirical relations. We find a cluster metallicity [Fe/H]ZW =-2.01 ± 0.04, or [Fe/H]UVES =-2.11 ± 0.06, and a distance of 8.32 ± 0.20 kpc (using RR0 variables), 8.10 kpc (using one RR1 variable), and 8.35 ± 0.42 kpc (using our SX Phoenicis star detection in M 30). Fitting isochrones to the CMD, we estimate an age of 13.0 ± 1.0 Gyr for M 30. © 2013 ESO
Althoefer, Anna M. (Death, 1873-09-30)
No full textAddress: WadeAge at death: 2 yrsPg 221/1873/2/F W S/City/Dr. Carney/Ackerman/St. JohnsOriginal record filed in drawer labeled'ALMS-ANDERSON'
Treating epilepsy in the presence of sleep disorders
No full textSleep disorders have an adverse impact on daily living for both children and their caregivers. Sleep disturbance and lack of restful sleep can masquerade as a myriad of clinical problems, including inattention, depression, headache, and seizures. A discussion highlighting differences between epilepsy and the most common sleep disorders during childhood is provided
Buffering in human tears: pH responses to acid and base challenge
No full textThe buffering capacity of tears collected from six young, healthy subjects was assessed using a microtitration technique. Each subject provided, on six separate occasions, about 100 microliter of tears, collected in small amounts and with minimal mechanical stimulation over several hours. The pH of the total stirred pool of tears from each subject was determined at the outset. This pool of tears was then divided into two equal volume aliquots, the pH of each being determined following each titration step of one of them with acid, and of the other with base. In all, 28 titration steps across the acid-base spectrum were completed for each patient pool collected. A total of 1044 tear pH measurements were made, all being done in a closed, temperature stabilized (36 degrees C) microelectrode chamber having an accuracy of within 0.04 pH units. For a comparative reference, an identical titration procedure was used on degassed, demineralized distilled water (348 pH determinations). Buffering capacity was found to show considerable intersubject variations, but in all cases the effect was more pronounced and more uniform following acid titration. Local zones of enhanced buffering across the pH spectrum could be identified, presumably reflecting the multiple buffering components (bicarbonate, protein and others) present in tear fluid
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