92 research outputs found
Long-term resveratrol treatment improves the capillarization in the skeletal muscles of ageing C57BL/6J mice
No full textWe recently showed that the treatment with Resveratrol (RES) contrasts the effects of ageing on the skeletal muscle (SKM), reduces the appearance of tubular aggregates (TAs), and improves the fatigue resistance. Since fatigue resistance depends on the SKM capillary network, and RES has been described to improve vascularisation, we analysed the SKM capillarization in naturally ageing C57BL/6J male mice, fed with 0.04% RES in the diet for 6 months, which showed a better fatigue resistance in a previous work. Our data show an inverse correlation between the number of capillaries per fibre (CAF) and TAs in both control and treated type IIB fibres, and an increase of CAF in ageing SKM upon RES-treatment. The present work suggests that capillarization is one of the determinants of the development of TAs and fatigue resistance, and that RES can be considered a good candidate to counteract capillary rarefaction in the SKM tissue
Resveratrol treatment reduces the appearance of tubular aggregates and improves the resistance to fatigue in aging mice skeletal muscles
No full textResveratrol (RES) is a polyphenolic compound found in grapes, peanuts, and in some berries. RES has been reported to exhibit antioxidant, anti-inflammatory, anti-proliferative properties, and to target mitochondrial- related pathways in mammalian cells and animal models. Therefore, RES is currently advised as supplement in the diet of elderly individuals. Although it is hypothesized that some of RES beneficial actions likely arise from its action on the skeletal muscle, the investigation of RES effects on this tissue remains still elusive. This study reports the effects of a 0,04% RES-supplemented diet for six months, on the skeletal muscle properties of C57/ BL6 aging mice. The analysis of the morphology, protein expression, and functional-mechanical properties of selected skeletal muscles in treated compared to control mice, revealed that treated animals presented less tubular aggregates and a better resistance to fatigue in an ex-vivo contraction test, suggesting RES as a good candidate to reduce age-related alterations in muscle
Le bun pha We:t des Lao du nord-est de la Thaïlande
No full textThe Bun Pha We:t of the Laotians of Northeast Thailand
by Bernard Formoso
Constructed around the recitation of the story of Vessantara, the next-to-the-last incarnation of the Buddha, the bun Pha Ve:t mingles aims which are spiritual (the acquisition of merit) and material (an abundant harvest), and observers such as Condominas and Tambiah have presented it as the most important of Laotian peasant festivals. The author confirms this point of view with new ethnographic information which makes it possible to refine the interpretation of the festival and place it more precisely in the religious calendar year. He shows that it integrates, in a single ritual complex, motifs which "are developed separately at other times of the year and that it makes of the organising community a temporary cosmic center toward which converge not only the representatives of surrounding villages, but also the many divinities of the upper and lower worlds who are invited for the occasion.Formoso Bernard. Le bun pha We:t des Lao du nord-est de la Thaïlande. In: Bulletin de l'Ecole française d'Extrême-Orient. Tome 79 N°2, 1992. pp. 233-260
One‐ and Two‐Electron Reductions in MiniSOG and their Implication in Catalysis**
No full textThe unconventional bioorthogonal catalytic activation of anticancer metal complexes by flavin and flavoproteins photocatalysis has been reported recently. The reactivity is based on a two-electron redox reaction of the photoactivated flavin. Furthermore, when it comes to flavoproteins, we recently reported that site mutagenesis can modulate and improve this catalytic activity in the mini Singlet Oxygen Generator protein (SOG). In this paper, we analyze the reductive half-reaction in different miniSOG environments by means of density functional theory. We report that the redox properties of flavin and the resulting reactivity of miniSOG is modulated by specific mutations, which is in line with the experimental results in the literature. This modulation can be attributed to the fundamental physicochemical properties of the system, specifically (i) the competition of single and double reduction of the flavin and (ii) the probability of electron transfer from the protein to the flavin. These factors are ultimately linked to the stability of flavin‘s electron-accepting orbitals in different coordination modes.We acknowledge the Basque Government – Eusko Jaurlaritza (IT1254-19, IT1584-22, IkasC-2021-1-0252 (A.Z.), PIBA_2021_1_0034 (L.S.)), University of the Basque Country UPV/EHU (PIF19/244), Spanish State Research Agency (PID2019-109111RB-I00 (L.S., E.R. O.A.), PGC2018-097529-B-100 (X.L., E.F.), and FPU20/00688 (O.A.)) and Diputación Foral de Gipuzkoa (RED 2021) for financial support and the SGi/IZO-SGIker UPV/EHU for generous allocation of computational resources. Prof. Jesus Ugalde is acknowledged for fruitful discussions about electron transfer processes. L.S. thanks the Spanish Multi-MetDrugs RED2018-102471-T. This work was performed under the Severo Ochoa Centres of Excellence Programme run by the Spanish State Research Agency, CEX2018-000867-S (DIPC)
Article: «Architectural Terracruda Sculptures of the Silk Roads: New Conservation Insights Through a Diagnostic Approach Based on Non-Destructive X-ray Micro-Computed Tomography» (2021)
No full textLópez-Prat M., Agostino R. G., Ray Bandyopadhyay S., Carrascosa B. Crocco M. C., De Luca R., Filosa R., Formoso V., Lancelotti C., Agha Noori N., Pecci A., Simón-Cortés S., Miriello D. (2021), Architectural Terracruda Sculptures of the Silk Roads: New Conservation Insights Through a Diagnostic Approach Based on Non-Destructive X-ray Micro- Computed Tomography, Studies in Conservation, DOI: 10.1080/00393630.2020.1862605 Abstract This work presents the results of the study of a fragment of ..
Estimation of direct and maternal genetic correlation of Birth Weight with its environmental maternal variability in mice
No full textInternational audienceEstimation of direct and maternal genetic correlation of Birth Weight with its environmental maternal variability in mice N. Formoso-Rafferty1, I. Cervantes1, J.P. Gutiérrez1, L. Bodin2 1Universidad Complutense de Madrid, Facultad de Veterinaria, Departamento de Producción Animal, Spain 2 INRA, GenPhySE, Castanet-Tolosan, France [email protected] (Corresponding Author) In polytocous species like pig, rabbit and mice, environmental variability of birth weight (BW) is generally studied assigning each BW record of a litter to the dam and considering that all genetic effects are maternal. However a direct effect might exist and could induce bias in the parameter estimations. In this paper, analyses of genetic parameters for the mean (location) and the variability (dispersion) of BW has been made on mice data from a divergently selection experiment on BW considering maternal genetic effects on the location and on the dispersion of BW together with a direct genetic effect on its location. They were done through a DHGLM procedure implemented in the ASReml software which could deal with both direct and maternal effects and which provided their variances and covariances for the location and the dispersion. The correlation between maternal genetic effects for the BW location and the BW dispersion was positive and confirmed results previously obtained. The new genetic correlation between a direct genetic effect on the location and the maternal genetic effect on the dispersion of BW was also positive. Thus increasing the individual BW, whatever the direct or maternal component concerned by the selection, should be avoided if BW variability is ignor
Estimation of direct and maternal genetic correlation of Birth Weight with its environmental maternal variability in mice
No full textInternational audienceEstimation of direct and maternal genetic correlation of Birth Weight with its environmental maternal variability in mice N. Formoso-Rafferty1, I. Cervantes1, J.P. Gutiérrez1, L. Bodin2 1Universidad Complutense de Madrid, Facultad de Veterinaria, Departamento de Producción Animal, Spain 2 INRA, GenPhySE, Castanet-Tolosan, France [email protected] (Corresponding Author) In polytocous species like pig, rabbit and mice, environmental variability of birth weight (BW) is generally studied assigning each BW record of a litter to the dam and considering that all genetic effects are maternal. However a direct effect might exist and could induce bias in the parameter estimations. In this paper, analyses of genetic parameters for the mean (location) and the variability (dispersion) of BW has been made on mice data from a divergently selection experiment on BW considering maternal genetic effects on the location and on the dispersion of BW together with a direct genetic effect on its location. They were done through a DHGLM procedure implemented in the ASReml software which could deal with both direct and maternal effects and which provided their variances and covariances for the location and the dispersion. The correlation between maternal genetic effects for the BW location and the BW dispersion was positive and confirmed results previously obtained. The new genetic correlation between a direct genetic effect on the location and the maternal genetic effect on the dispersion of BW was also positive. Thus increasing the individual BW, whatever the direct or maternal component concerned by the selection, should be avoided if BW variability is ignor
Olvidar la vida en el hogar: A razón de "Trance y memoria en el budismo y el yoga" de Raquel Ferrández Formoso
No full textThe purpose of this paper is to reflect on the work Trance and Memoryin Buddhism and Yoga by Raquel Ferrández Formoso, specifically on her contributionto the role of memory and attention in the Buddhism of the first centuries and in theYogasūtra. In her book, the author shows how the two main traditions analyzed proposethe substitution of an implicit samsaric memory by another nirvanic liberating memory,through forgetting home life and the consummation of the eternal-never-return. Onthe other hand, she shows how the new mindfulness meditation methodologies separatethemselves from the old meditative conceptions, since they propose a pure attention thatis not related to liberating philosophies and their ethics, to favor a sensualism in thepresent moment. Unlike the original Buddhism and the Yogasūtra that propose leavingbehind home life, mindfulness, focused on therapy, does not imply giving up the wayof life, or any profound philosophical change.El propósito de este escrito es reflexionar acerca de la obra Trance y memoria en el budismo y el yoga de Raquel Ferrández Formoso, en concreto sobre su aportación al papel de la memoria y la atención en el budismo de los primeros siglos y en los Yogasūtra. En su libro, la autora muestra cómo en las dos tradiciones principales analizadas se propone la sustitución de una memoria implícita samsárica por otra memoria liberadora nirvánica, mediante el olvido de la vida en el hogar y la consumación del eterno-nunca-retorno. Por otra parte, muestra cómo las nuevas metodologías de meditación mindfulness se separan de las concepciones meditativas antiguas, ya que propondrían una atención pura, autónoma respecto de las filosofías liberadoras y las éticas del budismo y de los Yogasūtra, para favorecer un sensualismo del momento presente. A diferencia del budismo originario y de los Yogasūtra que proponen dejar la vida en el hogar, el mindfulness, centrado en la terapia, no supone ninguna renuncia del modo de vida, ni ningún cambio filosófico profundo
The agroecological sustainability of petty farmers in Thailand: A challenge for the future
No full textSmallholder farming has faced significant transformations in Thailand over the past thirty years. These socio-demographic, technical, economic and climatic changes have considerably increased its vulnerability. The sustainability of the smallholders' agricultural model which rests on cash crops and requires large quantities of chemical inputs is now threatened. Through the study in 2014 and 2019 of two rural villages he had first surveyed in 1984–1985, the author offers a detailed review of these changes, and examines the ways the farmers deal with them
Deletion of small ankyrin 1 (sAnk1) isoforms results in structural and functional alterations in aging skeletal muscle fibers
No full textMuscle-specific ankyrins 1 (sAnk1) are a group of small ankyrin 1 isoforms, of which sAnk1.5 is the most abundant. sAnk1 are localized in the sarcoplasmic reticulum (SR) membrane from where they interact with obscurin, a myofibrillar protein. This interaction appears to contribute to stabilize the SR close to the myofibrils. Here we report the structural and functional characterization of skeletal muscles from sAnk1 knockout mice (KO). Deletion of sAnk1 did not change the expression and localization of SR proteins in 4- to 6-mo-old sAnk1 KO mice. Structurally, the main modification observed in skeletal muscles of adult sAnk1 KO mice (4-6 mo of age) was the reduction of SR volume at the sarcomere A band level. With increasing age (at 12-15 mo of age) extensor digitorum longus (EDL) skeletal muscles of sAnk1 KO mice develop prematurely large tubular aggregates, whereas diaphragm undergoes significant structural damage. Parallel functional studies revealed specific changes in the contractile performance of muscles from sAnk1 KO mice and a reduced exercise tolerance in an endurance test on treadmill compared with control mice. Moreover, reduced Qγ charge and L-type Ca(2+) current, which are indexes of affected excitation-contraction coupling, were observed in diaphragm fibers from 12- to 15-mo-old mice, but not in other skeletal muscles from sAnk1 KO mice. Altogether, these findings show that the ablation of sAnk1, by altering the organization of the SR, renders skeletal muscles susceptible to undergo structural and functional alterations more evident with age, and point to an important contribution of sAnk1 to the maintenance of the longitudinal SR architecture
- …
