64 research outputs found
Il parco museo Sant'Anna del Furlo. Land art tra conservazione e restauro
No full textTutto cominciò per caso e per curiosità, come si addice alle storie libere e innovative. La studentessa Lorenza Bagnarelli si trovò un giorno ad assistere ad una video-presentazione
della nostra esperienza della Casa degli Artisti. Erano le giornate di orientamento post-laurea organizzate dall’Università di Urbino. Lorenza ne rimase toccata e le germogliò
l’idea di farne oggetto della sua tesi. Cominciò un lavoro certosino della Bagnarelli, aiutata da tutto il collegio universitario della facoltà urbinate di Conservazione e Restauro dei Beni Culturali, in primis dal professor Elvio Moretti che ha “mappato” tutte le opere presenti nel Parco-Museo di Sant’Anna del Furlo, dotandole di GIS e di QrCode.
Iniziò quindi la conoscenza, le analisi, i questionari. Nell’aprile di quest’anno la Bagnarelli ha discusso la sua tesi nuova e originale, si è laureata a pieni voti, e dal quel giorno nacque l’idea di trarre dal suo lavoro un libro, che grazie alla Aras Edizioni, e alla cura del professor Moretti, possiamo mostrare con orgoglio. La questione principe è: si può
conservare e restaurare un’opera d’arte contemporanea, soprattutto quelle della Land Art, per loro natura, spesso, caduche? La coordinatrice della Scuola di Conservazione e Restauro dell’Università di Urbino, Laura Baratin°, sostiene che quando: “vengono prese in considerazione anche opere dell’arte contemporanea, una categoria davvero eterogenea per materiali, tecniche esecutive e percorso teorico-artistico che stimola un approccio, in caso di conservazione e restauro, particolarmente meditato e del tutto innovativo anche
per la tipologia di istituzioni, associazioni ed enti coinvolti, ci deve essere un continuo confronto dialettico tra i diversi specialisti, i restauratori, la direzione lavori, gli enti proprietari e gli studenti stessi. La possibilità di salvare opere inedite, che inevitabilmente avrebbero seguito un destino di degrado e di abbandono, offre l’opportunità agli studenti di misurarsi nella didattica, affrontando un lavoro completo: dalle prime fasi di conoscenza, di analisi, alle scelte di intervento, correttamente seguite dalla docenza e dalla Soprintendenza fino all’esposizione finale” . E così è stato. Oggi, dopo anni di ostinata volontà nel proporre una vetrina di “opere sotto il cielo”, non ci sentiamo più soli
Modeling lateral facies heterogeneity of an upper Oligocene carbonate ramp (Salento, southern Italy)
No full textThe aim of this work is to reproduce a metre-scale facies heterogeneity 3D model of the Chattian Porto Badisco Calcarenite carbonate ramp outcropping in the Salento Peninsula (southern Italy). However, in shallow-water carbonate systems, capturing metre-scale facies heterogeneity in three-dimensional models remains controversial due to the possibility of facies coexistence and because their association can change through time and space. Within this context, the continuous and well-exposed Chattian Porto Badisco Calcarenite carbonate ramp allows detailed study of the distribution of lithofacies association and their architecture along the dip direction depositional profile. The lithofacies and the depositional model of the Porto Badisco Calcarenite are referred to those defined by Pomar et al. (2014). The Porto Badisco Calcarenite is a homoclinal carbonate ramp with a euphotic inner setting characterised by the extensive seagrass meadows, passing basinward into a large rotaliid packstone and coral mounds developed in mesophotic conditions. The deeper part of the oligophotic zone is characterised by rhodolithic floatstone to rudstone and large lepidocyclinid packstone. The distal part of the ramp is characterise by a fine calcarenite. The methodology used in this work combines classical field data collection (e.g., stratigraphic logs and field-facies mapping) and 3D stochastic modeling by using PetrelTM. All the data (top and base of stratigraphic logs, cross-section, key surfaces, lithofacies lateral extension etc.) were georeferenced and inserted into the software to build the digital outcrop model. The 3D facies model has been performed after several simulations through specific stochastic algorithms (SISim, TGSim), comparing the models reproduce by the two algorithms, matching the depositional geometries and the lithofacies association observed in the outcrop. The 3D modeling represents a useful tool to better understand the facies architecture and their complex heterogeneity. Moreover, a detailed 3D facies model provides an essential tool to characterise semi-quantitatively sedimentological features for subsurface reservoir studies
The central proline rich region of POB1/REPS2 plays a regulatory role in epidermal growth factor receptor endocytosis by binding to 14-3-3 and SH3 domain-containing proteins
No full textBackground. The human POB1/REPS2 (Partner of RalBP1) protein is highly conserved in mammals where it has been suggested to function as a molecular scaffold recruiting proteins involved in vesicular traffic and linking them to the actin cytoskeleton remodeling machinery. More recently POB1/REPS2 was found highly expressed in androgen-dependent prostate cancer cell lines, while one of its isoforms (isoform 2) is down regulated during prostate cancer progression. Results. In this report we characterize the central proline rich domain of POB1/REPS2 and we describe for the first time its functional role in receptor endocytosis. We show that the ectopic expression of this domain has a dominant negative effect on the endocytosis of activated epidermal growth factor receptor (EGFR) while leaving transferrin receptor endocytosis unaffected. By a combination of different approaches (phage display, bioinformatics predictions, peptide arrays, mutagenic analysis, in vivo co-immunoprecipitation), we have identified two closely spaced binding motifs for 14-3-3 and for the SH3 of the proteins Amphiphysin II and Grb2. Differently from wild type, proline rich domains that are altered in these motifs do not inhibit EGFR endocytosis, suggesting that these binding motifs play a functional role in this process. Conclusion. Our findings are relevant to the characterization of the molecular mechanism underlying the involvement of POB1/REPS2, SH3 and 14-3-3 proteins in receptor endocytosis, suggesting that 14-3-3 could work by bridging the EGF receptor and the scaffold protein POB1/REPS2. © 2008 Tomassi et al; licensee BioMed Central Ltd
La educación emocional asistida por perros, como puente de bienestar en el aula
No full textLa realidad de nuestra sociedad exige que en el sistema educativo, el binomio cognición-emoción sea inseparable para formar a los alumnos de manera integral. Para ello resulta indispensable desarrollar la inteligencia emocional por medio de los procesos de enseñanza y aprendizaje, y el incremento de competencias socioemocionales a través de la educación emocional en las escuelas. Teniendo bien afianzadas las mismas es menos probable que se produzcan conductas disruptivas, sino contrariamente se fomenta el clima positivo y lleva al bienestar en las aulas. En base a la idea de conocer, expresar y encauzar adecuadamente las emociones, se incorporan perros en las prácticas de educación emocional donde se establecen vínculos con los alumnos que favorecen la dinámica de las actividades. En este marco intentamos analizar la contribución de la educación emocional asistida por perros en el contexto áulico para fortalecer el bienestar, la convivencia y apoyar y dinamizar los procesos de enseñanza y aprendizaje.Es a partir de aquí que específicamente describimos la experiencia del taller realizada en la escuela N°14 de la Ciudad Autónoma de Buenos Aires.Fil: Maison Baibiene, María Mercedes. Entre libros y hocicos; Argentina.Fil: Silva, Leticia. Entre libros y hocicos; Argentina.Fil: Tomassi, Anabella. Entre libros y hocicos; Argentina.Fil: Borrino, Laura. Entre libros y hocicos; Argentina
Inclusione di un estratto di farina di grano fermentata in liposomi per la somministrazione orale: effetto su cellule intestinali HT-29
No full textLisosan G (LG) è una farina di grano fermentata contenente polifenoli, acido alfa-lipoico, acidi grassi polinsaturi e vitamine con proprietà antiossidanti e antinfiammatorie (Gabriele et al., 2018). Questi costituenti sono spesso caratterizzati da instabilità chimica, bassa solubilità e biodisponibilità nonché scarso assorbimento, che ne limitano l'applicazione terapeutica. L‘impiego di liposomi, sistemi nanoparticellati che possono incorporare composti con diverse proprietà chimico-fisiche, consente di proteggere le componenti bioattive dalla degradazione, aumentarne la solubilità, modularne il rilascio e facilitarne la diffusione attraverso le membrane biologiche (Hendawy, 2021). In questo studio, abbiamo formulato e caratterizzato liposomi contenenti l'estratto acquoso di LG per la somministrazione orale. È stato utilizzato il polimero enterico Eudragit® per proteggere i liposomi dall'ambiente gastrico, e sono state ottenute vescicole unilamellari di circa 100 nm. È stata quindi valutata la Ioro stabilità in fluidi gastrointestinali simulati, la Ioro potenziale tossicità nelle cellule intestinali umane HT-29, tramite saggio MTT e gli effetti sulla produzione di specie reattive dell'ossigeno (ROS) intracellulari, rilevati in seguito all'esposizione delle cellule alla DCFH-DA, una sonda fluorescente permeabile alla membrana cellulare. I risultati hanno mostrato gastro-resistenza degli Eudragit-liposomi, che non hanno alterato la vitalità delle cellule HT-29, non differendo tra LG libero e LG internalizzato nel liposoma. lnoltre, gli Eudragit-liposomi non hanno indotto variazioni dei livelli intracellulari dei ROS. I liposomi rappresentano pertanto un sistema efficiente per la veicolazione di estratti ottenuti da alimenti complessi e, se formulati correttamente, possono offrire protezione dalla degradazione fisiologica senza effetti tossici sulle cellule intestinali
Morphological and biochemical changes in myelin subfractions of developing rats fed microbial lipids
No full textMorphological, biochemical, and physicochemical studies of myelin subfractions were undertaken of the progeny of Sprague-Dawley rats fed diets containing lipid either extracted from yeasts grown on n-alkanes or from margarine. Myelin subfractions obtained from pooled brain homogenates of littermates by sucrose density gradient centrifugation at 7, 14, and 21 days postnatally were subjected to electron microscopy, sodium dodecylsulfate polyacrylamide gel electrophoresis and assayed for 2',3'cyclic nucleotide 3'-phosphohydrolase activity (CPNase; EC 3.1.4.37). Additionally, surface pressure measurements were made of lipid monolayers derived from myelin subfractions, which were subsequently injected with myelin basic proteins. The myelin subfractions of test animals, when compared with those of controls, shown an earlier increase in the specific activity of CNPase, the earlier appearance of low-molecular-weight proteins, and an increase in the affinity of basic proteins for lipids derived from the myelin light fraction. This biochemistry suggests the presence of a more mature myelin between 7 and 14 days in the experimental group. The morphological studies, however, do not seem to concur with the biochemical data. The observed changes are discussed in relation to the influence of dietary lipids on myelinogenesis
¿Sirve la investigación básica? : editorial
No full textEditorial: ¿Sirve la investigación básica? por Erina PetreraNuevos enfoques: Síndrome de Rett: una enfermedad del neurodesarrollo de diagnóstico esquivo por María Belén Cardillo, Bruno Gabriel Berardino, Eduardo Tomás CánepaRevisión: Optimización del tratamiento con antidepresivos en adultos por Astrid Montserrat Olivares Morales, Jazmín Hernández Valdez, Aldo Velázquez ZepedaCaso clínico: Porfiria aguda intermitente: presentación de caso por Laura Sabina Varela, Viviana Alicia Melito, Marcelo Guolo, Fabiana Alejandra Caballero, Verónica Goñi, María del Carmen Martínez, María Pasman, Valeria Paccioli, Lucía Tomassi, Ana María Buzaleh, Victoria Estela Parer
Tackling the challenges of the oral administration of a fermented flour extract with liposomes: Effect on intestinal HT-29 cells
No full textLisosanG is a fermented flour that contains polyphenols, polyunsaturated fatty acids, vitamins, and alpha-lipoic acid with antioxidant/anti-inflammatory properties. These compounds are often characterized by instability, low solubility/bioavailability, and poor absorption that restrict their application in therapy. These problems can be solved by using delivery systems among which liposomes have emerged as prominent candidates. Liposomes can load compounds with varied physico-chemical properties, providing protection from degradation, increased solubility, modulation of release, and facilitated diffusion across biomembranes. Lisosan G liposomes tailored for oral administration were prepared. Eudragit® enteric polymer was added to protect the liposomes from acidic pH. The Eudragit-liposomes were around 100 nm and unilamellar. They were resistant to acidic conditions and did not alter cell viability, nor intracellular ROS levels. These findings confirm that liposomes are an efficient system for the loading of multicomponent-based extracts and that, when properly formulated, can offer protection from physiological degradation, and be safely applied to cells.The authors wish to thank Agrisan Srl for providing Lisosan G. Jaume Caelles, from the SAXS-WAXS service at IQAC, is acknowledged for helping with the SAXS determinations.Peer reviewe
Disasters and catastrophes
No full textInternational audienceThe chapter retraces the birth and development of disaster research in the social sciences and critically discusses the main frameworks that have emerged and driven disaster studies over the past 50 years: vulnerability and, more recently resilience and preparedness. Where the vulnerability approach revealed since the 1970s the role of social inequalities in explaining disasters, the paradigms of resilience and preparedness reflect the increasing pervasiveness of the theme of the catastrophe that has accompanied the emergence of the Anthropocene. They sustain an ongoing process of re-technicization and de-politicization in disaster research. Against this tendency, critical approaches to the study of disasters investigate the structural link between disasters and capitalism and between capitalism and the ecological crisis. The final section identifies some emerging issues: the growing intertwining between disasters and technological innovation, the generalization of the argument of exceptionality and the emergence of forms of mobilizations motivated by 'climate catastrophism'
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