663 research outputs found
Anti - tumorigenic assessment of HELA cell growth characteristics in the presence of Meswak, 2016
No full textThere is a demand for newer, more effective therapeutic antagonist for bacterial threats. More specifically, overuse of currently available antibiotics, premature dosage -termination of prescription antibiotics and a growing resistance of bacteria to currently available antibiotics warrants the design, development and establishment of new and novel antibacterial therapeutics. Unfortunately, physician-scientists no longer have an antibiotic agent for an increasing number of multiple strains of bacteria. In this study, we investigate the holistic efficacy for which the plant Meswak can antagonize the vitality of some strains of bacteria and HeLa cell growth. The conclusions drawn from the findings of this investigation suggest Meswak as a potential antagonist for the growth of some strains of gram-positive and gram-negative bacteria. Preliminary studies have also revealed that Meswak acts as an inhibitor to HeLa cell growth and migration. Future studies are necessary to define the specific mechanism of action for which Meswak acts to inhibit bacterial viability and HeLa cell growth and migration
Extraction of Ethanolic Extract of Red Betel Leaves and Its Cytotoxicity Test on HeLa Cells
No full textAbstractExtraction and identification of active compounds in red betel leaves (Piper crocatum Ruiz&Pav.) have been conducted. Ethanolic extract of red betel leaves was obtained by Soxhlet extraction followed by solvent removal step using rotary evaporator. Compound identification of the extract was performed by GCMS. The concentrated ethanol extract was then examined through cytotoxicity test on Hela cells. Results from GCMS spectrum depicted several compounds which were identified in the extract, i.e. neophytadiene, elimicin and propionic acid. Cytotoxicity test was conducted on Hela cells using in vitro method and calculated using Probit method. LC50 value from the ethanolic extract of red betel leaf was 0.81 + 0.26mg/ml. This result showed that the bioactive compounds potentially inhibited proliferation of Hela cells
Opposed to the being of Henrietta: bioslavery, pop culture and the third life of HeLa cells
No full textOperating at the intersection of thanatopolitics and African-American cultural studies, this essay argues that the commercial sale of HeLa-themed art and other bioproducts perpetuates the bioslavery of HeLa cells, a circumstance created by legal and medical discourses tracing back to US racial slavery. Racial slavery normalised economic, social and legal inequities that the nation continues to struggle with and, the article posits, laid foundation for the dynamics that currently exist between Henrietta Lacks' genealogical family, the HeLa cell line, and the medical-pharmaceutical establishment. The author turns to fashion ethics discourse and trademark law as potential sites for reparations
Validation of a Strategy for Cancer Therapy: Delivering Aminoglycoside Drugs to Mitochondria in HeLa Cells
No full textMitochondria in human cancer cells have been implicated in cancer cell proliferation, invasion, metastasis, and even drug-resistance mechanisms, making them a potential target organelle for the treatment of human malignancies. Gentamicin (GM), an aminoglycoside drug (AG), is a small molecule that functions as an antibiotic and has ototoxic and nephrotoxic characteristics. Thus, the delivery of GM to mitochondria in cancer cells would be an innovative anticancer therapeutic strategy. In this study, we attempted mitochondrial delivery of GM in HeLa cells derived from a human cervical cancer. For the mitochondrial delivery, we used MITO-Porter, a liposomal nanocarrier for mitochondrial delivery via membrane fusion. We first encapsulated GM in the aqueous phase of the carrier to construct GM-MITO-Porter. Flow cytometry analysis and fluorescent microscopy observations permitted us to confirm that the GMeMITO-Porter was efficiently taken up by HeLa cells and accumulated in mitochondria, whereas naked GM was not taken up by the cells. Moreover, cell viability assays using HeLa cells showed that the GMeMITO-Porter induced strong cytotoxic effects related to mitochondrial disorder. This finding is the first report of the mitochondrial delivery of an AG to cancer cells for cancer therapeutic strategy. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved
Small-angle neutron scattering (SANS) and spinecho SANS measurements reveal the logarithmic fractal structure of the large-scale chromatin organization in HeLa nuclei
No full textThis paper reports on the two-scale fractal structure of chromatin organization in the nucleus of the HeLa cell. Two neutron scattering methods, small-angle neutron scattering (SANS) and spin-echo SANS, are used to unambiguously identify the large-scale structure as being a logarithmic fractal with the correlation function (r) - ln(r/E). The smaller-scale structural level is shown to be a volume fractal with dimension DF = 2.41. By definition, the volume fractal is self-similar at different scales, while the logarithmic fractal is hierarchically changed upon scaling. As a result, the logarithmic fractal is more compact than the volume fractal but still has a rather high surface area, which provides accessibility at all length scales. Apparently such bi-fractal chromatin organization is the result of an evolutionary process of optimizing the compactness and accessibility of gene packing. As they are in a water solution, the HeLa nuclei tend to agglomerate over time. The large-scale logarithmic fractal structure of chromatin provides the HeLa nucleus with the possibility of penetrating deeply into the adjacent nucleus during the agglomeration process. The interpenetration phenomenon of the HeLa nuclei shows that the chromatin-free space of one nucleus is not negligible but is as large as the volume occupied by chromatin itself. It is speculated that it is the logarithmic fractal architecture of chromatin that provides a comfortable compartment for this most important function of the cell.</p
Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis
No full textFluorescence lifetime images of HeLa cells expressing enhanced green fluorescent protein (EGFP) have been measured, as apoptosis is induced by tumor necrosis factor-α (TNF-α) in combination with cycloheximide. The fluorescence lifetime of EGFP is found to decrease after the induction of apoptosis, indicating that the change in environment occurs around the chromophore of EGFP with the apoptosis process. The fluorescence lifetime imaging technique can be used to perform in vivo observation of cell death processes. Fluorescence lifetime measurements are useful to examine the induction of the apoptosis process, even when a morphological change of each cell cannot be observed because of a low spatial resolution
The secretion inhibitor Exo2 perturbs trafficking of Shiga toxin between endosomes and the trans-Golgi network
No full textThe small-molecule inhibitor Exo2 {4-hydroxy-3-methoxy-(5,6,7,8-tetrahydrol[1]benzothieno[2,3-d]pyrimidin-4-yl)hydraz-one benzaldehyde} has been reported to disrupt the Golgi apparatus completely and to stimulate Golgi–ER (endoplasmic reticulum) fusion in mammalian cells, akin to the well-characterized fungal toxin BFA (brefeldin A). It has also been reported that Exo2 does not affect the integrity of the TGN (trans-Golgi network), or the direct retrograde trafficking of the glycolipid-binding cholera toxin from the TGN to the ER lumen. We have examined the effects of BFA and Exo2, and found that both compounds are indistinguishable in their inhibition of anterograde transport and that both reagents significantly disrupt the morphology of the TGN in HeLa and in BS-C-1 cells. However, Exo2, unlike BFA, does not induce tubulation and merging of the TGN and endosomal compartments. Furthermore, and in contrast with its effects on cholera toxin, Exo2 significantly perturbs the delivery of Shiga toxin to the ER. Together, these results suggest that the likely target(s) of Exo2 operate at the level of the TGN, the Golgi and a subset of early endosomes, and thus Exo2 provides a more selective tool than BFA for examining membrane trafficking in mammalian cells
Analysis Of Textual Meaning and Melodic Structure Of Popular Batak Toba Songs During The Presentation Of Ulos Hela In Batak Toba Wedding Ceremonies In Medan
No full textUlos Hela is usually given by the bride’s parents to the couple.These three songs are always chosen to be sung before the Ulos Hela is presented to the bride and groom. Before presenting the Ulos Hela, the bride’s parents usually deliver advice and dedicate one of the songs, such as Burju Marsimatua, Gabe Maho Boru, or Boru Nabasa.To analyze the textual meaning in this study, the author uses Ferdinand de Saussure’s semiotic theory, focusing on denotative and connotative meanings. In addition, the songs Burju Marsimatua, Gabe Maho Boru, and Boru Nabasa contain advice and prayers with connotative meanings that reflect the social values of the Batak Toba community. The research techniques used are fieldwork and desk work as introduced by Bruno Nettl. The author applies a qualitative method through observation, interviews, and audio-video recording. The findings of this study indicate that the Ulos Hela ceremony is a crucial highlight of the Batak Toba wedding tradition, symbolizing parental love, blessings, and the transfer of responsibility from the bride’s parents to the groom’s family. The songs Burju Marsimatua, Gabe Maho Boru, and Boru Nabasa convey prayers, advice, and moral values that guide the newlyweds in building a harmonious household. The melodic analysis shows that all three songs use the strophic formula, are dominated by the 1P interval, and have a pendulous contour, creating a solemn atmosphere. This tradition serves as a medium for preserving cultural identity and instilling noble values for future generations.82 pagesSkripsi Sarjan
Author Correction: H3K64 trimethylation marks heterochromatin and is dynamically remodeled during developmental reprogramming
No full textIn this article, the Ponceau staining presented in Fig. 1b (right, bottom) does not follow best practices for figure preparation since itinadvertently included duplications from the Ponceau staining presented in Supplementary Fig. 1b (for which the same preparation ofnucleosomes from HeLa cells had been used). A new Fig. 1b is provided in the Author Correction
Northern blotting for miR-21 in Hela cells after transfection of 2′-O-MOE-PS miR-21 ASO
No full text<p><b>Copyright information:</b></p><p>Taken from "Improved targeting of miRNA with antisense oligonucleotides"</p><p>Nucleic Acids Research 2006;34(8):2294-2304.</p><p>Published online 11 May 2006</p><p>PMCID:PMC1459537.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> miR-21 ASO or a control ASO were transfected into Hela cells and levels of miR-21 were measured 24 h later. A control experiment in which miR-21 ASO was spiked into Hela cell lysates showed that the ASO did not interfere with miR-21 detection (spike). Cells mock transfected with low-serum media only (Optimem) or with media containing the transfection reagent (Lipofectin) also showed no reduction in miR-21 levels
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