1,721,161 research outputs found
Patch Clamp Technique
Get PDFThis book is a stimulating and interesting addition to the collected works on Patch clamp technique. Patch Clamping is an electrophysiological technique, which measures the electric current generated by a living cell, due to the movement of ions through the protein channels present in the cell membrane. The technique was developed by two German scientists, Erwin Neher and Bert Sakmann, who received the Nobel Prize in 1991 in Physiology for this innovative work. Patch clamp technique is used for measuring drug effect against a series of diseases and to find out the mechanism of diseases in animals and plants. It is also most useful in finding out the structure function activities of compounds and drugs, and most leading pharmaceutical companies used this technique for their drugs before bringing them for clinical trial. This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach. This book will be of good value for students of physiology, neuroscience, cell biology and biophysics
Underlying Mechanisms of Epilepsy
No full textThis book is a very provocative and interesting addition to the literature on Epilepsy. It offers a lot of appealing and stimulating work to offer food of thought to the readers from different disciplines. Around 5% of the total world population have seizures but only 0.9% is diagnosed with epilepsy, so it is very important to understand the differences between seizures and epilepsy, and also to identify the factors responsible for its etiology so as to have more effective therapeutic regime. In this book we have twenty chapters ranging from causes and underlying mechanisms to the treatment and side effects of epilepsy. This book contains a variety of chapters which will stimulate the readers to think about the complex interplay of epigenetics and epilepsy
Underlying Mechanisms of Epilepsy
Get PDFThis book is a very provocative and interesting addition to the literature on Epilepsy. It offers a lot of appealing and stimulating work to offer food of thought to the readers from different disciplines. Around 5% of the total world population have seizures but only 0.9% is diagnosed with epilepsy, so it is very important to understand the differences between seizures and epilepsy, and also to identify the factors responsible for its etiology so as to have more effective therapeutic regime. In this book we have twenty chapters ranging from causes and underlying mechanisms to the treatment and side effects of epilepsy. This book contains a variety of chapters which will stimulate the readers to think about the complex interplay of epigenetics and epilepsy
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Entamoeba invadens; a study about the role of PLD1 and PLD2 in encystation
Get PDFOne of the most common problem in the developing world is infectious diseases caused by poor living conditions and one such condition is called amoebiasis. The causative agent of which is Entamoeba histolytica; a microaerophilic, parasitic amoebozoan species, which causes intestinal and extraintestinal amoebiasis in humans. Although there are certain drugs available for treating this disease, more research is needed to come up with novel drugs. Furthermore, basic research is needed to understand the different stages of its life cycle and possible therapies during its dormant stages. One such stage is the encystation of its cell, a crucial stage which basically involves the transformation of motile trophozoite into resistant cyst. It is extremely difficult to treat cysts, and a growing body of research has shown that phospholipase D (PLD) activity is significantly increased in Entamoeba histolytica during encystation, indicating that it plays a critical role in cyst formation. PLD is a widely distributed enzyme in all branches of life that hydrolyzes phosphatidylcholine. It is believed to play an important role in the regulation of cell physiology by extracellular signals such as hormones, neurotransmitters, growth factors, and cytokines. Due to our inability to effectively induce encystation in vitro in Entamoeba histolytica, our knowledge about the cyst form remains limited. This also hampers our ability to develop cyst-specific diagnostic tools. This thesis is about role of PLD during cyst formation in Entamoeba. In this study we attempted to investigate a potential role of PLD during encystation in the laboratory model organism Entamoeba invadens as encystation is not experimentally achievable in human parasite Entamoeba histolytica. The research included amplification of PLD1 and PLD2 genes by extraction of genomic DNA and cloning of the genes into standard cloning vector pGEM-T-Easy and then in the Entamoeba-specific vector pEiNEO. Electroporation of the cloned Entamoeba specific vectors was performed by transfecting the Entamoeba invadens trophozoite cells. On other hand, encystation activity was also checked by introducing 0.6% n-butanol and t-butanol in 50% LYG media (encystation medium) but it did not show any significant inhibition. Fluorescent microscopy results showed no effect of n-butanol on cysts formation. Bioinformatics investigation showed that the HKD active site motif of PLD was found conserved throughout Entamoeba species as in animal, plants, and fungi
فسادات کے پس منظر میں لکھے گئے اردو افسانے: خاندانی مسائل کی پیشکش
No full textWith the migration to Pakistan in 1947, many social problems surfaced. While Urdu short stories depicted other problems and other aspects of the migration, many short stories also portrayed the problems faced by families. This article studies the familial problems caused by migration as depicted in Urdu short stories.
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