135 research outputs found
Francisco de Quevedo y Antonio Sancho Dávila y Toledo Colonna, III marqués de Velada
No full text[Resumen] Los posibles contactos entre la familia de Quevedo y la casa de Velada desde tiempos de Felipe II permiten ahondar en la relación entre Francisco de Quevedo y el III marqués de Velada. Para ello estudiamos la jocosa carta sobre el viaje de Felipe IV a Andalucía en 1624. Estas relaciones entre Quevedo y la nobleza nos descubren la posición que ocupa el escritor respecto al conde duque de Olivares.[Abstract] The possible contacts between the family of Quevedo and the family of Velada dating from the times of Felipe II, allow us to study the relations between Francisco de Quevedo and the 3rd Marquis of Velada. In order to do this, we study the humourous letter about the travel of Felipe IV to Andalusia in 1624. These relations between Quevedo and noblemen discover the positon of the writer vis-à-vis the Conde Duque de Olivare
Crónica de los festejos con que la Universidad ha celebrado el segundo centenario del fallecimiento de D. Pedro Calderón de la Barca
No full text7 ej. de la misma obraBHR/C-038-009 (15) Enc. Rúst.BHR/C-041-007 (24) Enc. Rúst.Discursos y poesías leidas en alabanza del egregio poeta en solemne sesión literaria que tuvo lugar en el paraninfo de la Universidad. Letra de himnos cantados en la velada literaria y artística que se dió en el teatro de Isabel La Católic
Expression Profile of PIN-Formed Auxin Efflux Carrier Genes during IBA-Induced In Vitro Adventitious Rooting in Olea europaea L.
No full textExogenous auxins supplementation plays a central role in the formation of adventitious roots (AR) for several plant species. However, the molecular mechanisms underlying the process of adventitious rooting are still not completely understood and many plants with economic value, including several olive cultivars, exhibit a recalcitrant behavior towards cutting propagation, which limits its availability in plant nurseries. PIN-formed proteins are auxin efflux transporters that have been widely characterized in several plant species due to their involvement in many developmental processes including root formation. The present study profiled the expression of the OePIN1a-c, OePIN2b, OePIN3a-c, OePIN5a-c, OePIN6, and OePIN8 gene members during indole-3-butyric acid (IBA)-induced in vitro adventitious rooting using the olive cultivar ‘Galega vulgar’. Gene expression analysis by quantitative real time PCR (RT-qPCR) showed drastic downregulation of most transcripts, just a few hours after explant inoculation, in both nontreated and IBA-treated microcuttings, albeit gene downregulation was less pronounced in IBA-treated stems. In contrast, OePIN2b showed a distinct expression pattern being upregulated in both conditions, and OePIN5b was highly upregulated in IBA-induced stems. All transcripts, except OePIN8, showed different expression profiles between nontreated and IBA-treated explants throughout the rooting experiment. Additionally, high levels of reactive oxygen species (ROS) were observed soon after explant preparation, decreasing a few hours after inoculation. Altogether, the results suggest that wounding-related ROS production, associated with explant preparation for rooting, may have an impact on auxin transport and distribution via changes in OePIN gene expression. Moreover, the application of exogenous auxin may modulate auxin homeostasis through regulation of those genes, leading to auxin redistribution throughout the stem-base tissue, which may ultimately play an important role in AR formation
Expressão das proteínas da matriz na discondroplasia da tíbia
No full textDoutoramento em BiologiaA discondroplasia da tíbia (TD) em aves consiste numa anomalia do esqueleto
onde existe uma falha nos processos normais da ossificação endocondral.
Esta patologia é caracterizada pela formação de uma cartilagem não
vascularizada e não mineralizada que se estende até à metáfise. Uma vez que
existem várias anomalias do esqueleto em mamíferos com lesões semelhantes
às apresentadas pela TD, este trabalho teve como objectivo a caracterização
desta patologia em termos das moléculas que podem estar envolvidas no seu
desenvolvimento. Assim, foi estudada a expressão das macromoléculas da
matriz extracelular, das enzimas degradadoras da matriz (metaloproteinases
da matriz: MMPs), bem como das moléculas envolvidas na proliferação e
diferenciação celular, na angiogénese e apoptose. A expressão génica foi
realizada, por PCR quantitativo em tempo real, em placas de crescimento
normais e discondroplásicas obtidas a partir de frangos de carne (broilers) da
estirpe Cobb. Os níveis proteicos de algumas MMPs foram analisados por
immunoblotting e zimografia de gelatina. No presente estudo não se verificou
alteração na expressão dos genes dos colagénios do tipo II, IX, X e XI, bem
como do agrecano, nas lesões discondroplásicas. Observou-se uma redução
acentuada nos níveis de mRNA da gelatinase-B (MMP-9), da colagenase-3
(MMP-13) e das estromalisinas -2 (MMP-10) e -3 (MMP-11), bem como nos
níveis proteicos da gelatinase-A (MMP-2) e da MMP-13. Por outro lado, a
MMP-7 aumentou drasticamente a expressão do seu gene. As moléculas
envolvidas na proliferação e diferenciação dos condrócitos, tais como a PTHrP,
o Ihh, o Cbfa-1 e o Sox-9, mantiveram a sua expressão génica nas lesões. Por
outro lado, o TGF-β reduziu a sua expressão. A caspase-3 também dimimuiu a
sua expressão na patologia. Em relação aos factores angiogénicos, o FGF
manteve a sua expressão e o VEGF aumentou significativamente nas lesões.
Este aumento do VEGF juntamente com o aumento da MMP-7 sugere um
aumento da hipoxia nas lesões. Os nossos resultados sugerem que a
acumulação da cartilagem observada na discondroplasia é devida a uma
diminuição da proteólise da matriz, resultado de uma sub-expressão das
MMPs, e não de um aumento da produção das macromoléculas da matriz.
Desta forma, os nossos resultados sugerem que a falha na expressão e/ou
activação das MMPs poderá estar associada ao desenvolvimento da
discondroplasia da tíbia em aves. Finalmente, os nossos resultados vêm
suportar os resultados anteriores que sugerem uma ligação entre a expressão
das MMPs e anomalias no processo de ossificação endocondral.Avian tibial dyschondroplasia (TD) is a skeletal disease where the normal
events of endochondral bone formation are disrupted. It is characterized by the
formation of a lesion composed of nonvascularized and nonmineralized
cartilage that can extend into the metaphysis. Because there are several
mammalian skeletal diseases with lesions similar to TD, the present work
aimed to characterize the disease in terms of the molecules that may be
involved in its development. Thus, the expression of extracellular matrix (ECM)
macromolecules, ECM-degrading enzymes (matrix metalloproteinases: MMPs),
and the regulatory molecules involved in cell proliferation and differentiation,
angiogenesis, and apoptosis, were studied. Gene expression was performed
by real-time quantitative PCR in normal and TD-affected growth plates from 3-
week-old broiler chicks (Cobb strain). The protein levels of some of the MMPs
studied were analysed by immunoblotting and gelatin zymography. The
collagen types II, IX, X, and XI as well as aggrecan did not change their gene
expression in dyschondroplastic lesions. There was a pronounced reduction in
the mRNA levels of gelatinase-B (MMP-9), collagenase-3 (MMP-13), and of
stromelysins-2 (MMP-10) and -3 (MMP-11), as well as in the protein levels of
gelatinase-A (MMP-2) and MMP-13. On the other hand, MMP-7 mRNA has
increased significantly. The molecules involved in chondrocyte proliferation and
differentiation, such as PTHrP, Ihh, Cbfa-1, and Sox-9 have maintained their
mRNA levels in the pathology. On the other hand, TGF-β has decreased its
gene expression. Caspase-3 also showed diminished mRNA levels in the
pathology. Regarding the angiogenic factors, FGF has maintained its
expression and VEGF has increased significantly in the lesions. The increment
in VEGF in conjunction with the increased expression of MMP-7 suggests the
formation of a hypoxic environment in the lesions. Our results suggest that the
accumulated cartilage observed in dyschondroplasia seems to be the result of
decreased matrix proteolysis due to the downregulation of MMPs and not to an
increased production of the matrix macromolecules. Thus, our results suggest
that the failure in the expression or lack in the activation of MMPs might be
associated with the development of avian tibial dyschondroplasia. Furthermore,
our results strengthen the link between the lack in MMP expression and
abnormal endochondral bone formation.FCT - SFRH/BD/6420/200
Antonia de Mendoza, dama de palacio, condesa de Benavente y poeta del siglo XVII
No full textAntonia de Mendoza (¿?-Madrid 1656), countess of Benavente per her marriage to Juan Francisco Alfonso Pimentel de León, X conde de Benavente, wrote verses that barely reached the printing. Some of those are collected in a handwritten volume of Poesías varias with the work of other amateur poets of the time. The objective of this article is to revise the figure and work of the countess of Benavente in the context of the courts of the Queens Isabel de Borbón and Mariana de Austria, to whom she served as lady-in-waiting, study her relationships with the nobles, among them the III marquis of Velada, and reconsider the attribution of various poems.Antonia de Mendoza (¿?-Madrid 1656), condesa de Benavente tras su matrimonio con Juan Francisco Alfonso Pimentel de León, X conde de Benavente, escribió versos que apenas llegaron a la imprenta. Algunos de ellos están recogidos en un volumen manuscrito de Poesías varias junto a la obra de otros poetas aficionados de la época. El objetivo del presente trabajo es revisar la figura y la obra de la condesa de Benavente en el contexto de las cortes de las reinas Isabel de Borbón y Mariana de Austria, a las que sirvió como dama de palacio, estudiar la red de relaciones que trabó con diferentes nobles, entre ellos con el III marqués de Velada, y replantear la atribución de varios poemas
UMA PEDAGOGIA TEATRAL VELADA: A ÜBER-MARIONETTE DE GORDON CRAIG
No full textThe pedagogical aspect of Gordon Craig’s thought is not an explicit feature of his work in general, which seems to prefer weave considerations over aesthetic issues and the search for a redefinition of what is the actual language of theatre as an art. But insinuates itself subtly in his early writings - sometimes in the opposite direction - and will slowly surfacing towards formalizing a theoretical and practical pedagogical model for an "artist of the future." In this educational approach of his work, we can spot the creation of the allegory of the Über-marionette, and its scepticism about the actor’s work, the main target of criticism included in this formulation. Combining this imaginative creation of a utopian super-actor and his attributes, with a detailed description of the impracticability actor as author and artistic material, gives us an intriguing educational proposal that interexchange with their aesthetic vision and form a kind of veiled pedagogy, hidden in ambiguities and in poetic images, tying in perfectly with the lyrical universe of all his work. O caráter pedagógico do pensamento de Gordon Craig não é um aspecto explícito de sua obra de um modo geral, que parece preferir debruçar-se sobre questões estéticas e de redefinição do que seja a própria linguagem da arte do teatro. Mas se insinua de maneira sutil em seus primeiros escritos - por vezes numa via contrária - e irá aflorando lentamente em direção à formalização de um modelo pedagógico teórico e prático para um “artista do teatro do futuro”. Nesse viés pedagógico de seu trabalho se insere a criação da alegoria do Über-marionette e a crítica ao trabalho do ator, alvo principal da crítica inserida nessa formulação. A combinação dessa imaginativa criação utópica de um super-ator e seus atributos, com uma minuciosa descrição da inviabilidade do ator como autor e material artístico, rascunha uma intrigante proposta pedagógica que dialogará com suas questões estéticas e formará uma pedagogia velada, oculta em duplos sentidos e imagens poéticas, que se coadunam perfeitamente com o universo lírico de toda sua obra
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L.
No full textOlive (Olea europaea subsp. europaea var. europaea L.) comprises several cultivars with reduced capacity to be propagated due its recalcitrant behaviour upon adventitious rooting (AR) stimulus. This prevents their propagation and consequently their availability in the nurseries. By overcoming the difficult-to-root behaviour it will be possible to increase the number of cultivars available for new orchards and consequently taking profit of it on final products differentiation. There are many protocols used in vegetative propagation to induce AR based on auxins, a group of phytohormones largely known as involved in many processes of plant development, including root initiation and development. However, most of these protocols are still based on “trial/error” approaches, where several variables needs to be tested. This happens because genetic control underlying AR is not completely elucidated. Auxins are mainly synthesized in young leaves and apical meristem of shoots and roots. The major auxin distribution is regulated by transport from cell to cell, known as polar auxin transport (PAT). PAT is mediated by three main classes of membrane auxin transporters, the auxin resistant 1/like aux1 (AUX/LAX), the ATP binding cassette subfamily B (ABCB/MDR/PGP) and the pin-formed (PIN) carriers. The PIN gene family encodes a subgroup of auxin efflux carriers shown to be involved in various developmental processes, including lateral/adventitious root formation, in several plant species. To date, PIN genes have been identified in 31 plant species by genome-wide approaches, however, there is still no information regarding its identification in olive. The recent publication of O. europaea genome allowed us to perform the identification of all members belonging to PIN gene family in this species (OePIN). Our work aims to characterize OePIN family, as well as, to investigate the involvement of its members during AR. The expression profile study of OePIN members during AR in IBA-induced in vitro cultured microshoots of cv. ‘Galega vulgar’, attempting to understand if the hard-rooting behaviour of this cultivar might be related with a disturbance in auxin transport. Additionally, reactive oxygen species (ROS) as key signaling molecules that regulate growth and development and coordinate responses to biotic and abiotic stresses in plants are also being analysed in IBA-treated and non-treated explants. Also, cells from phloem, cortex and sub-epidermis are being isolated and gene expression will be performed on these cells in order to find out which cells are responsible for the formation of adventitious roots
Anne Waldman (La Elegía)
No full textLa noche del cinco de abril de 1997, el poeta Allen Ginsberg muere en brazos de la poeta Anne Waldman. Waldman logra capturar el espíritu beat durante unas horas, las necesarias para velar el cadáver. Su misión consiste en congregar uno por uno a cuantos poetas y demás criaturas líricas, vivas o muertas, sean capaces de producir una elegía beatnik en honor del poeta muerto. Anne Waldman impulsa la velada con su fuerza escénica de poeta y performer, y como anfitriona perfecta, se erige en guardiana del cuerpo de Ginsberg hasta la madrugada
Artes de canto (1492-1626) y mujeres en la cultura musical del mundo ibérico renacentista
No full text[spa] Esta Tesis Doctoral explora la cultura musical del mundo ibérico renacentista a través del estudio de artes de canto en lengua vernácula impresas entre 1492 y 1626 y de su relación con las mujeres de la época. La Tesis consta de siete capítulos, estructurados en dos partes (Vol. I), y de veinte apéndices (Vol. II). La primera parte (Capítulos I al IV) muestra que las características por las que estos libros que contenían los rudimentos de la música han sido infravalorados hasta ahora por la historiografía musical son precisamente las mismas que demuestran que respondían a una necesidad pedagógica y a la demanda de manuales asequibles, breves y prácticos por parte de un amplio mercado. Las artes de canto se imprimieron en tiradas de miles de ejemplares y eran vendidas a bajo precio, tuvieron una enorme circulación en la Península Ibérica y el Nuevo Mundo, y contribuyeron al incremento y la difusión de la educación musical en contextos educativos diferenciados (la iglesia, la universidad y el ámbito privado), así como entre grupos sociales hasta entonces excluidos del aprendizaje de los fundamentos de la música. El Arte de canto llano (Sevilla, 1530) de Juan Martínez emerge como el tratado de música del mundo hispánico más difundido geográfica y cronológicamente en el siglo XVI e inicios del XVII, pero del que casi nada se sabía. En la segunda parte (Capítulos V al VII), las conexiones entre estos libros de música y mujeres muestran que las áreas de superposición entre lo privado y lo público y entre lo oral y lo escrito permiten desafiar la invisibilidad de las mujeres en documentos históricos y vislumbrar trazas no sólo de la cultura musical de las mujeres de la época, sino también de la importancia de la música en la vida cotidiana. Se utiliza una diversidad de fuentes (artes de canto, libros de conducta, documentos inquisitoriales, literatura, correspondencia e inventarios de bienes, entre otras), a través de las cuales se ha podido documentar la relación con la música de, entre otras, Catalina de Zúñiga, VI Condesa de Lemos, Isabel de Plazaola, e Isabel de Aragón, IV Duquesa del Infantado. Empleando metodologías de la musicología tradicional junto a otras tomadas de los historiadores del libro y de la cultura popular, esta Tesis Doctoral presenta una panorámica de la vida musical de la época a través del prisma de doble alteridad que supone el estudio de las artes de canto, generalmente consideradas carentes de interés, y de su relación con las mujeres, insuficientemente representadas en la historiografía musical.[eng] This dissertation explores the musical culture of the Renaissance Iberian world through both the study of small-format treatises in the vernacular containing the rudiments of music –known as artes de canto– printed between 1492 and 1626, and the nexuses between them and women. The dissertation consists of seven chapters, structured into two parts (Volume I), and twenty appendixes (Volume II). Part I (Chapters I to IV) shows that the arte de canto, until now generally overlooked or undervalued in music historiography, was produced in print runs of thousands of copies and sold for a low price; it had a broad circulation in the Iberian Peninsula and the New World, contributing to the spread of musical literacy in distinct didactic contexts (churches, universities, private settings) and among social groups until then excluded from learning the rudiments of music. The little known Juan Martínez’s Arte de canto llano (Seville, 1530) emerges as the most circulated music book in the Hispanic world during the sixteenth and seventeenth centuries. The aim of Part II (Chapters V to VII) is to approach the musical life of the sixteenth-century Iberian world through the exploration of women’s contributions, thus broadening the field of historical research. The study of some connections between music books and women shows that the points of overlap between the private and the public spheres, on the one hand, and the written and the oral music transmission, on the other hand, not only make women’s musical practices visible, but also offer new vistas on the popular culture of the age. Through a variety of source materials (artes de canto, conduct manuals, Inquisition records, literature, letters and inventories of goods, among others) it has been possible to document the musical activities of women such as Catalina de Zúñiga, VI Countess of Lemos, Isabel de Plazaola, and Isabel de Aragón, IV Duchess of the Infantado. Combining methodologies from traditional musicology with those borrowed from book history and popular culture, this dissertation analyzes music in the culture of the Renaissance Iberian world through the prism of double Otherness involved in studying the ‘other’ music books –that is the undervalued artes de canto– and their connections to women of that period
El Vejamen de Antonio Hurtado de Mendoza en Sevilla (1624) y su relación con una Carta de Quevedo
No full textEl autor de este artículo propone estudio y la edición de un texto inédito de don Antonio Hurtado de Mendoza, el Vejamen que se iba a dar en Sevilla a unos grados en que había de asistir el Rey Felipe IV, en marzo de 1624. El texto es interesante porque se relaciona directamente con una famosa carta del autor del Buscón, escrita justo en este mismo año 1624, y con la contestación a la misma de su destinatario, el Marqués de Velada.
The author of this article suggests the edition and study of an unpublished text by Antonio Hurtado de Mendoza, a Vejamen that was about to be played in Seville before the king Philip IV, in March 1624. The text is also important because it is directly related to a famous Quevedo’s letter written just in the same year and with the reply of his addressee, the marquis of Velada
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