1,592 research outputs found
Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc
No full textHP1 is a major component of chromatin and regulates gene expression through its binding to methylated histone H3. Most eukaryotes express at least three isoforms of HP1 with similar domain architecture. However, despite the common specificity for methylated histone H3, the three HP1 isoforms bind to different regions of the genome. Most of the studies so far focused on the HP1a isoform and its role in transcriptional regulation. As HP1a requires additional factors to bind methylated chromatin in vitro, we wondered whether another isoform might also require additional targeting factors. Indeed, we found that HP1c interacts with the DNA binding factors Woc and Row and requires Woc to become targeted to chromatin in vivo. Moreover, we show that the interaction between HP1c and Woc constitutes a transcriptional feedback loop that operates to balance the concentration of HP1c within the cell. This regulation may prevent HP1c from binding to methylated heterochromatin
Speed of Publication of Statutes and Regulations in the United Kingdom, Canada, and the United States
No full textExamines reasons for typical delays in publication of statutes & regulations in UK,US, & Canada. Finds US federal laws published more slowly because of time taken to add marginal notes after enactmen
Legal Citation Form: Theory and Practice
No full textSets forth 13 principles as a basis for a system of legal citation forms, and critically reviews various rules in the 13th edition of A Uniform System of Citation
Normative reconstruction and social character of freedom in Axel Honneth
No full textO presente objeto de pesquisa busca proceder ao estudo e identificação dos traços essenciais envolvidos na abordagem teórica das relações sociais e políticas trazidas na obra O Direito da Liberdade do filósofo alemão Axel Honneth. Faz-se uma análise da influência hegeliana sobre o conceito de liberdade, assim como dos fatores relacionados com o suprimento das carências subjetivas, mediadas pelas diferentes “esferas” sociais. Honneth, assim, procura trazer à tona a compreensão de um novo modelo de liberdade advindo da Filosofia do Direito de Hegel, o qual se distingue substancialmente dos modelos tradicionais. O autor busca evidenciar a limitação das teorias da justiça de tradição liberal, invocando a necessidade de uma visão integrada das relações sociais experimentadas nas esferas referidas por Hegel, concebendo-se uma experiência concreta de liberdade social. Nesse sentido, evidencia-se o caráter interdisciplinar e emancipatório do método de reconstrução normativa como base teórica para a justificação pública nas sociedades modernas.This research object aims to study and identify the essential traits involved in the theoretical approach of social and political relations brought in the work Freedom’s Right by the german philosopher Axel Honneth. It analyses the hegelian influence on the concept of freedom, as well as the factors related to the supply of subjective needs, mediated by the different social "spheres". Honneth thus seeks to bring to light the understanding of a new model of freedom stemming from Hegel’s Philosophy of Law, which differs substantially from traditional models. The author seeks to highlight the limitation of liberal theories of justice, invoking the need for an integrated view of the social relations experienced in the spheres referred to by Hegel, conceiving a concrete experience of social freedom. In this sense, the interdisciplinary and emancipatory character of the normative reconstruction method is evidenced, as a theorical basis for public justification in modern societies
Use of Microscale Thermophoresis (MST) for Studying binding interactions of PRSet-7/SETD8 with small molecule specific inhibitors EPI-9 and EPI-23.
No full textHistone methylation plays a key role in establishing and maintaining stable gene expression patterns during cellular differentiation and embryonic development. Considering that a number of small molecules identified as modulators of methyltransferases by high-throughput screening were dyes or derivatives, a small focused library of dye-like compounds was prepared and the small molecules synthesized were pre-screened for potential inhibition of histone lysine methyltransferases (HKMT) using in vitro HMT assays. Two compounds, EPI-
9 and EPI-23, showed low IC50 values against nucleosomal HKMT PR-Set7. Prompted by our interest in the study of small molecule modulators of these epigenetic targets, we applied MST (Microscale Thermophoresis) to the investigation of the interaction of PRset7 with small molecule ligands EPI-9 and EPI-23. Microscale thermophoresis (MST) is a new method that enables the quantitative analysis of molecular interactions in solution. MST is the directed movement of particles in a microscopic temperature gradient: any change of the hydration
shell of biomolecules due to changes in their structure/conformation results in a relative change of movement along the temperature gradient and is used to determine binding affinities, binding kinetics and activity kinetics. Events such as the binding of small molecules to a target can be monitored by this tecnique
Supplementary scripts for Kiss et. al (2023)
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<p>These are the supplementary scripts for the article:</p>
<p>Anna E Kiss, Anuroop V Venkatasubramani, Dilan Pathirana, Silke Krause, Aline Campos Sparr, Jan Hasenauer, Axel Imhof, Marisa Müller, Peter B Becker, Processivity and specificity of histone acetylation by the male-specific lethal complex, <em>Nucleic Acids Research</em>, 2024;, gkae123, <a href="https://doi.org/10.1093/nar/gkae123">https://doi.org/10.1093/nar/gkae123</a></p>
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The glyoxalase system as an example of a cellular maintenance pathway with relevance to aging
No full textCommentary on: Scheckhuber CQ et al. Modulation of the glyoxalase system in the aging model Podospora anserina: effects on growth and lifespan. Aging. 2010; 2:969-980
A categoria reconhecimento na teoria de Axel Honneth
No full textARAÚJO NETO, José Aldo Camurça de. A categoria reconhecimento na teoria de Axel Honneth. Argumentos, Fortaleza, v. 3, n. 5, p. 139-147, 2011.This article analyzes the category in recognition of Axel Honneth theory. To this end, the author
uses written juveniles of Hegel in Jena. During this period, Hegel develops a theory of
intersubjectivity that encourages the development of term recognition as a backdrop where
conflicts. This context, that Axel Honneth develops its critical theory in his struggle for Recognition.O presente artigo tem como objetivo analisar a categoria reconhecimento na teoria de Axel
Honneth. Para tanto, o autor recorre aos escritos juvenis de Hegel no período de Jena. Neste
período, Hegel desenvolve uma teoria da intersubjetividade que propicia a elaboração do
termo reconhecimento como um pano de fundo onde se dão os conflitos. É neste contexto
que Axel Honneth desenvolve sua teoria crítica em sua obra Luta por Reconhecimento
A LIBERDADE REFLEXIVA SEGUNDO AXEL HONNETH / REFLECTIVE FREEDOM ACCORDING TO AXEL HONNETH
No full textO presente artigo dedica-se ao estudo do modelo de liberdade reflexiva segundo o pensamento de Axel Honneth, exposto no capítulo A.II (A Liberdade Reflexiva e sua Concepção de Justiça) da obra Direito da Liberdade, realizado através do método hipotético-dedutivo e da técnica de pesquisa bibliográfica, a fim de verificar porque a reflexão do indivíduo realizada sobre os seus próprios atos e guiada apenas por suas intenções não representa o modelo orientador da concepção de justiça proposto pelo autor.The present article intends to study the model of reflective freedom according to the thought of Axel Honneth, exposed in chapter A.II (The Reflective Freedom and its Conception of Justice) of the work Law of Freedom, realized through the hypothetic-deductive method and the bibliographical technique research, in order to verify why the individual reflection carried out on his own acts and guided only by his intentions does not represent the guiding model of the conception of justice proposed by the author
Projection methods and scenarios for public and private pension information
No full textPublic pensions – the primary pillar of old-age income provision – will, in the future, be less generous than they have been in the past, in particular owing to the impact of demographic change. The pension gap is supposed to be plugged by the second and third pillars of pension provision. However, people require reliable planning information if they are to exercise greater individual responsibility. It is therefore absolutely essential that adequate information is made available about the level of pension benefits that will be generated by each pillar of old-age pension provision. This paper outlines a number of different means of presenting the level of future pensions and the assumptions on which such extrapolations are necessarily based. Our work is based on an assumed average rate of inflation of 1.5% and an average rate of real income growth not exceeding 1.5%. This last figure is derived from calculations made in the framework of a macroeconomic simulation model. This model also shows that while the funded pillar of oldage pension provision is not entirely immune to population aging, it is not substantially threatened by a substantial decrease in stock market prices, the so-called “asset meltdown”.
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