9,984 research outputs found
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility
No full text14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants
CTQ2007-68014-C02-02 to CG and BIO2009-10964 to
MO); Fundacion Marcelino Botin (grant to MO);
Canadian Institutes for Health Research (grant to
M.J.D.); Natural Sciences and Engineering Research
Council of Canada (postgraduate scholarship to
J.K.W.). Funding for open access charge: Canadian
Institutes for Health Research
¿Qué Pasa, OSU? (Spring 2003)
No full textIssue includes: "Status of Studies: Update on Latino Studies" by Ignacio Corona; "La noche latina" by Nicole DeGreg; "El futuro eres tu" by Ney Arias; "El Plan Columbus: Twenty Five Years Later" by Ana María Quevedo; "Jose M. Castro, Ph.D." by Nicole DeGreg; "Claudio Gonzalez, Ph.D." by Nicole DeGreg; "Para comer La Bamba..." by Erica Lerma; "Open Your Eyes...and Your Mind" by José Antonio Carranza; "Welcome to Ohio State: Alpha Psi Lambda & UCHO Host Inner City Students Visiting from Chicago" by Ana María Quevedo; "La Clinica Latina: Providing Vital, Free Community Services" by Raquel Raíces and "La clinica latina: ofreciendo servicios gratuitos para la comunidad" by Raquel Raíces.Music, Art, Culture and History: An ECAP Recap "They Came and Changed Us" -- Faculty Excellence Profiles: Jose M. Castro, Ph.D. and Claudio Gonzalez, Ph.D. -- La Clinica Latina: Offering Services - in English, Ofreciendo Servicios - en Español -- Latino Studies Update: Esquina del Editor
Synergistic effects between analogs of DNA and RNA improve the potency of siRNA-mediated gene silencing
No full textWe report that combining a DNA analog (2?F-ANA) with rigid RNA analogs [2?F-RNA and/or locked nucleic acid (LNA)] in siRNA duplexes can produce gene silencing agents with enhanced potency. The favored conformations of these two analogs are different, and combining them in a 1–1 pattern led to reduced affinity, whereas alternating short continuous regions of individual modifications increased affinity relative to an RNA:RNA duplex. Thus, the binding affinity at key regions of the siRNA duplex could be tuned by changing the pattern of incorporation of DNA-like and RNA-like nucleotides. These heavily or fully modified duplexes are active against a range of mRNA targets. Effective patterns of modification were chosen based on screens using two sequences targeting firefly luciferase. We then applied the most effective duplex designs to the knockdown of the eIF4E binding proteins 4E-BP1 and 4E-BP2. We identified modified duplexes with potency comparable to native siRNA. Modified duplexes showed dramatically enhanced stability to serum nucleases, and were characterized by circular dichroism and thermal denaturation studies. Chemical modification significantly reduced the immunostimulatory properties of these siRNAs in human peripheral blood mononuclear cells. <br/
Slab-mantle flow interaction: influence on subduction dynamics and duration
No full textWe investigate the influence of mantle flow relative to the lithosphere on subduction dynamics. We use 2D thermo-mechanical models assuming incompressible non-Newtonian fluid rheology. Different mantle flow velocities consistent with absolute plate motion models are tested, as well as both directions of flow, either sustaining or opposing slab dip. The effects of different inflow/outflow velocity profiles, slab strengths and upper-lower mantle viscosity contrasts are also evaluated. Slab dip deviations between models with opposite mantle flow directions range from 37 for relatively strong slabs (eta(max) = 10(25) Pa s) to 50 for weaker slabs (eta(max) = 10(24) Pa s), accounting for a significant amount of natural slab dip variability. For imposed mantle flow supporting the slab, the initial stage of slab steepening is followed by a stage of continuous slab dip decrease. This slab shallowing eventually leads to mantle wedge closure, subduction cessation and slab break-off, possibly driving subduction flips
sj-pdf-1-lan-10.1177_00236772211043978 - Supplemental material for Integrated rearing system proposal for <i>Cantareus aspersus</i> in experimental orchards: Growth models
No full textSupplemental material, sj-pdf-1-lan-10.1177_00236772211043978 for Integrated rearing system proposal for Cantareus aspersus in experimental orchards: Growth models by Anton Garcia, Cecilio Barba, Manuel Aragón, Jose M León, Carmen De-Pablos-Heredero and Ana Gonzalez in Laboratory Animals</p
Viajantes ex-cêntricas nas histórias de Ana Miranda
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura.No conjunto das narrativas ficcionais da escritora brasileira Ana Miranda, a temática da viagem # considerada uma das mais férteis da literatura ocidental de todos os tempos # ocupa um espaço de centralidade, podendo até mesmo ser vista como o mais importante eixo de estruturação de suas obras. O estudo realizado pela presente tese tem por objetivo analisar o tratamento dado ao tema da viagem nos romances Desmundo, O retrato do rei, Dias & Dias e Amrik, evidenciando que por intermédio das narradora-viajantes # Oribela, Mariana, Feliciana e Amina # a autora promove um diálogo entre diferentes culturas, gêneros, etnias e gerações, ao mesmo tempo em que estabelece um profícuo diálogo com o passado em sua invariante problematização concernente aos limites e cruzamentos entre o discurso ficcional e os discursos narrativos extraliterários que o cercam, sobretudo o histórico e o biográfico. In the whole of Brazilian writer Ana Miranda#s fictional narrative, the travel thematic # considered one of the most fertile themes of the western literature of all times # occupies a central space, and can even been seen as the most important structuring axis of Miranda#s works. The aim of the present thesis is to analyze the treatment given to the travel subject in the novels Desmundo, O retrato do rei, Dias & Dias and Amrik, emphasizing that, through the traveling narrators, Oribela, Mariana, Feliciana and Amina, the author promotes a connection between different cultures, genders, ethnics and generations, at the same time that establishes a profitable dialogue with the past in her invariant problematization concerning the limits and crossings between the fictional and the extra-literary narrative discourses that surrounds it, mainly the historical and the biographic ones
Calorimeters - ATLAS Fact Sheet [Spanish]
No full textSpanish translation of the ATLAS Fact sheet about the ATLAS Calorimeters. Translation done by Daniel E. Trujillo M. and reviewed by Rebeca Gonzalez Suarez
The Inner Detector - ATLAS Fact Sheet [Spanish]
No full textSpanish translation of the Fact sheet about the Inner Detector. Translation done by Daniel E. Trujillo M. and reviewed by Rebeca Gonzalez Suarez
Detector Overview - ATLAS Fact Sheet [Spanish]
No full textSpanish translation of the Fact sheet giving an overview of the ATLAS detector. Translation done by Daniel E. Trujillo M. and reviewed by Rebeca Gonzalez Suarez
Muon Spectrometer - ATLAS Fact Sheet [Spanish]
No full textSpanish translation of the ATLAS Fact sheet about the Muon Spectrometer. Translation done by Daniel E. Trujillo M. and reviewed by Rebeca Gonzalez Suarez
- …
