67 research outputs found
ISOLASI, AKTIVITAS ANTIMIKROBA, KARAKTERISASI BAKTEROSIN DAN IDENTIFIKASI MOLEKULAR 16S rRNA BAKTERI ASAM LAKTAT DARI DADIH ASAL KABUPATEN SOLOK SUMATERA BARAT
No full textPengawetan makanan adalah hal yang penting yang menjadi perhatian dalam
proses industri makanan mengingat adanya kontaminasi dengan bakteri patogen
(Muhialdin, 2012). Bakteri ini, terutama bakteri anaerob dan anaerob fakultatif,
walaupun mereka ada dalam jumlah kecil, bakteri ini dapat berkembang biak
meskipun dalam suhu yang rendah dalam lemari pendingin, sehingga dapat
menimbulkan resiko terhadap keamanan makanan mentah atau produk roti. Banyak
pengawet makanan kimiawi yang mentargetkan bakteri dan jamur yang tumbuh pada
makanan telah digunakan bertahun-tahun. Dan akhir-akhir ini masyarakat
mengharapkan suatu produk tanpa pengawet kimia tapi mampu menjaga keamanan
dan masa konsumsi makanan (Muhialdin, 2011).
Biopreservasi makanan menggunakan bakteriosinogenik Bakteri Asam Laktat
(BAL) yang diisolasi langsung dari makanan adalah suatu terobosan yang inovatif
(Yang et al, 2012). BAL dikenal sebagai mikroorganisme yang tergolong GRAS
(Generally Recognized As Safe) dan memegang peranan penting dalam fermentasi
dan mempunyai potensi dalam pengawetan makanan (Yang et al, 2012). BAL
merupakan suatu kelompok bakteri Gram positif, tidak membentuk spora, berbentuk
kokus atau batang dan menghasilkan asam laktat sebagai produk hasil samping utama
selama fermentasi karbohidrat (Rattanachaikunsopon dan Phumkachorn, 2010)
PENGANTAR TEKNIK LABORATORIUM MIKROBIOLOGI DAN PENGENALAN BAKTERI ASAM LAKTAT
No full textMikroba adalah organisme yang berukuran mikro sehingga untuk mengamatinya diperlukan alat bantu berupa mikroskop. Mikroba dipertimbangkan sebagai salah satu sumber penghasil senyawa bioaktif yang ideal. Dalam ilmu sains, mikroba memiliki “posisi” khusus untuk dipelajari, terutama dalam bidang ilmu biologi dan biokimia. Di bab awal buku ini membahas sejarah penemuan mikroba , termasuk beberapa penelitian terkait “teori generasi spontan” yang kemudian berhasil dibantah oleh beberapa ilmuan. Dalam buku ini juga dibahas sejarah bakteri dimasa awal bumi terbentuk dan juga keragaman serta klasifikasi bakteri. Dari bab pertama ini, para pembaca akan diberikan pemahaman dasar mengenai mikroba dari aspek sejarah hingga klasifikasi. Buku ini juga menjelaskan mengenai metode isolasi bakteri dan laboratorium pendukung. Dalam bab ini, pembaca akan disajikan deskripsi teknik dasar penelitian dengan bakteri, termasuk penjelasn mengenai media pertumbuhan bakteri, cara-cara mengisolasi bakteri hingga instrument pendukun laboratorium mikrobiologi. Bab ini membantu memberikan deskripsi bagi para pembaca bagaimana konsep penelitian dengan bakteri sehingga sangat cocok dibaca bagi mahasiswa bidang fokus mikrobiologi yang akan melakukan penelitian. Pada bab berikutnya buku ini menyajikan teknik identifikasi bakteri, meliputi teknik Gram staining, karakterisasi biokimia, morfologi hingga konsep identifikasi bakteri secara molekuler. Dibidang mikrobiologi, identifikasi mikroorganisme penyebab infeksi memegang peranan yang sangat penting. Hal ini berkaitan dengan ketepatan terapi, pencegahan transmisi, serta pencegahan terjadinya resistensi antimikroba. Buku ini juga membahas sedikit perkenalan dengan bakteri asam laktat (BAL). BAL merupakan salah satu kelompok mikroba yang paling banyak dipelajari karena senyawa bioaktifnya memberikan banyak dampak positif terhadap kesehatan manusia. Senyawa bioaktif yang dimaksud adalah “bakteriosin”, yang merupakan suatu protein ekstraselular BAL. Bakteriosin telah cukup banyak yang dikomersilkan dalam dunia kesehatan. Pun demikian, diharapkan peneliti dimasa yang akan datang dapat lebih mengeksplorasi BAL untuk pemanfaatan senyawa bioaktifnya dibidang kesehatan dan kedokteran
Strong Antimicrobial Acvitity of Lactic Acid Bacteria Isolated from Raw Goat Milk in Medan, North Sumatera, Against Staphylococcus aureus
No full textBiopreservation is a method of foods preservation using natural ingredients by inhibiting the growth of spoilage food bacteria. Lactic acid bacteria is a bacteria that utilize as food preservation because it produce antimicrobial agent including bacteriocin and also act as probiotic. LAB could isolated from various natural source including raw milk. The aim of the study was to determined the antimicrobial activity of lactic acid bacteria (LAB) isolated from raw goat milk against food spoilage bacteria including Escherichia coli, Staphylococcus aureus, and Salmonella typhi. The result of the research indicated that the LAB isolated from raw goat milk(isolate EH1, EH2, EH3, EH4, EH5 and EH6) were Gram positive, homofermentative and negative in catalase test. The antimicrobial activity of LAB against indicator bacteria showed the strong antimicrobial activity against Staphylococcus aureus. The inhibition zone of EH1, EH2, EH3, EH4, EH5 and EH6 against S.aureus respectively were 19 mm ; 17,4 mm; 21,7 mm; 14,3 mm; 14,2 mm and 16 mm. The LAB isolates could not inhibit the growth of E.coli and S.typhi. It concluded that LAB isolated from raw goat milk has a potential as source of effective probiotic against Gram positive bacteria.
Pengaruh Keong Mas (Pomacea canaliculata) Sebagai MOL dan Lama Perendaman Terhadap Pertumbuhan Mucuna bracteata
No full textGinting OE, Pratomo B, Anggraini S, Fachrial E, Novita A. 2020. Effect of golden snail (Pomacea canaliculata) as MOL and soaking time on the growth of mucuna bracteata. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-8 Tahun 2020, Palembang 20 Oktober 2020. pp. xx. Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Golden snail (Pomacea canaliculata) is a pest on rice plants. Golden snails (Pomacea canaliculata) contain minerals that can be used as nutrients for plants so that they can be used as MOL (local microorganisms). The purpose of this study was to determine the effect of giving MOL snails and the duration of immersion on the growth of Mucuna bracteata. This study used a factorial randomized block design experimental method with 2 replications. The first factor was the concentration of MOL snails (K) with 4 levels of treatment, namely K0 (0%), K1 (15%), K2 (30%), K3 (45%) while the second factor was immersion time (M) with 4 levels of treatment M0 (0 minutes), M1 (15 minutes), M2 (30 minutes), M3 (45 minutes). The results showed that the interaction between the concentration of MOL snails (Pomacea canaliculata) and the immersion time did not have a significant effect on all parameters. Various concentrations of MOL snail (Pomacea canaliculata) and immersion time did not significantly affect the growth of Mucuna bracteata
ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT
No full textThe aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic
ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT
No full textThe aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic
Skrining Probiotik Asal Produk Komersil yang Berpotensi Sebagai Inhibitor a-Glukosidase
No full textVarious types of food products have good and bad effects on human health. One type of food that provides positive benefits is functional food. Functional foods are foods or beverages that are not only consumed to fulfill nutritional needs, but also provide good effects on the body's health and help reduce the risk of serious diseases. An example of functional food is probiotic drinks, which are known to support digestive health and strengthen the immune system. The content of probiotic drinks has a good impact on digestion and is able to inhibit α-glucosidase, so it is good to be consumed as a supplement for type 2 diabetes patients. The research focused on testing the potential of probiotics derived from commercial products on α-glucosidase enzyme activity. This type of research is laboratory experimental research with In Vitro method. The samples in this study are beverage samples with various types of brands containing probiotic milk that will be tested. The results showed that of the 8 probiotic isolates isolated from commercial products L-Bio, Yakult, Biokul and Max biotic have tolerance to acid and bile salt. Probiotic isolates in this study did not show antimicrobial activity. Screening of probiotics from commercial products with the most potential as α-glucosidase inhibitors was yakult
Pengaruh Bonggol Pisang (Musa Balbisiana Colla) sebagai MOL dan Lama Perendaman terhadap Pertumbuhan Mucuna bracteata
No full textAriyska D, Pratomo B, Sitinjak RR, Karosekali SA, Pinem JL, & Fachrial E. 2020. The effect of banana weevil (Musa balbisiana Colla) as MOL and soaking time on the growth of mucuna bracteata. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-8 Tahun 2020, Palembang 20 Oktober 2020. pp. xxx. Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).This study aims to determine the effect of concentration and soaking time of MOL banana weevil (Musa balbisiana Colla) on the growth of Mucuna bracteata. The banana species used in this study were bananas with seeds, and grew in abundance in several plantation areas. So that the potential is very disturbing to the main crop, namely oil palm, in other words it becomes a weed in plantations. In this study, bananas were used as a source of MOL. This study used an experimental method with a factorial randomized block design with 2 replications and 16 treatments. The first factor is the concentration of MOL banana weevil (Musa balbisiana Colla) (M) with 4 levels: M0 (0%), M1 (15%), M2 (30%), M3 (45%), the second factor is immersion time (P) with 4 levels: P0 (immersion), P1 (15 minutes), P2 (30 minutes), P3 (45 minutes). The data obtained were analyzed by means of analysis of variance with a significance of 5%. The results of data processing showed that anova did not significantly affect the interaction between the MOL concentration of banana weevil (Musa balbisiana Colla) and the immersion time on the growth of Mucuna bracteata. Various concentrations of banana weevil MOL (Musa balbisiana Colla) and soaking time did not significantly affect the growth of Mucuna bracteata plants. However, physically the growth of Mucuna bracteata is good, so that the presumption arises that this MOL actually acts as a nutrient/fertilizer for plants. Not as a growth regulator as the initial target of the research
NILAI KEARIFAN LOKAL DALAM CERITA RAKYAT
No full textPuji syukur penulis panjatkan kehadiran Allah SWT yang telah memberikan kesempatan kepada penulis sehingga dapat menyelesaikan penulisan monograf yang berjudul” Nilai Kearifan Lokal dalam Cerita Rakyat” ini dengan tepat waktu.Keberhasilan penyusunan buku monograf ini tentunya bukan atas usaha penulis sendiri saja namun ada banyak pihak yang turut membantu dan memberikan dukungan untuk selesainya penulisan buku ini. Untuk itu, penulis mengucapkan terima kasih yang sebesar-besarnya kepda:1. Dr. Chrismis Novalinda Ginting, SSit, MKes, Rektor Fakultas Keguruan danPendidikan Universitas Prima Indonesia yang telah memfasilitasi penulisan monograf.2. Edy Fachrial, S.Si. M.Si., Ketua LPPM Universitas Prima Indonesia yang telah memfasilitasi penulisan monograf.3. Dr.E. Rusiadi, S.E., M.Si., CIQaR.,CIQnR. yang telah berkenan menyediakan waktunya sebagai narasumber penelulisan monograf di Universitas Prima Indonesia.4. Kemenristekdikti yang telah membiayayai penelitian Dosen Pemula pada tahun 2020.Buku yang disusun oleh penulis tentunya masih ada kekerungan. Oleh karena itu, diperlukan masukan, kritik, dan saran dari pembaca untuk perbaikan isinya
HARGA PEMBELIAN TIKET
No full textPuji syukur kehadirat Tuhan Yang Maha Esa, karena kasih karuniaNya sehingga penulis boleh menyelesaikan penyusunan buku monograf ini tepat waktu. Adapun judul dari buku monograf ini adalah “Harga Pembelian Tiket”Dalam penyusunan dan penulisan buku monograf ini penulis menerima bantuan dari berbagai pihak, sehingga pada kesempatan ini penulis mengucapkan terima kasih kepada:1. Unpri Press atas penerbitan Buku monograf tersebut.2. Rektor UNPRI Prof. Dr. Chrismis Novalinda Ginting,M.Kes.3. Ketua LPPM UNPRI Edy Fachrial, S.Si., M.Si., CIQaR4. Pemateri Penulisan Buku Monograf Dr. E. Rusiadi, S.E., M.Si., CIQaR., CIQnR5. Para rekan-rekan Dosen sejawat yang terlibat langsung dalam penyusunan buku monograf ini.Penulis mengharapkan saran dan kritik yang boleh menambah kemampuan penulis dalam menulis, kiranya buku monograf ini boleh berguna bagi orang yang membacanya
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