72 research outputs found
Leishmania infantum in tigers and sand flies from a leishmaniasis-endemic area, southern Italy
No full textFrom March to November 2019, we detected Leishmania infantum infection in tigers (45%) and sand flies (5.3%) in a zoo in southern Italy. These infections in tigers and the abundance of Phlebotomus perniciosus sand flies represent a potential risk to other animals and humans living in or visiting the zoo. The role of tigers as “sentinels” and the possible risks for animals and humans are discussed
Invasive mammalian wildlife and the risk of zoonotic parasites
No full textInvasive wild mammals are present in all continents, with Europe, North America, and the Asian-Pacific region having the largest number of established species. In particular, Europe has been the continent with the highest number of zoonotic parasites associated with invasive wild mammals. These invasive species may represent a major threat for the conservation of native ecosystems and may enter in the transmission cycle of native parasites, or act as spreaders of exotic parasites. Here, we review the role of invasive wild mammals as spreaders of zoonotic parasites, presenting important examples from Europe, America, and the Asia-Pacific region. Finally, we emphasize the need for more research on these mammals and their parasites, especially in areas where their monitoring is scantily performed
Species-specific PCR assay for the detection of Babesia odocoilei
No full textWe developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei
Description of Joyeuxiella pasqualei (Cestoda: Dipylidiidae) from an Italian domestic dog, with a call for further research on its first intermediate host
No full textAlthough Joyeuxiella pasqualei is frequently detected in cats from Mediterranean Europe, information on its biology is still scarce. This cestode is relatively less frequently reported in dogs, possibly because it is often misdiagnosed with the better-known Dipylidium caninum. The occurrence of J. pasqualei proglottids in a dog living in a closed environment triggered us to delve into the biology of this cestode by collecting biological samples from lizards and a road-killed cat. Two reptile species, Podarcis siculus (Lacertidae), and Tarentola mauritanica (Geckonidae) were also collected in the garden and its surroundings. In addition, experimental infections with eggs obtained from gravid proglottids were performed in laboratory mice, and Tenebrio molitor (Coleoptera: Tenebrionidae) beetles. Proglottids from the dog's feces and adult cestodes detected at necroscopy of a cat were morphologically identified as J. pasqualei. Two out of 13 T. mauritanica collected in the garden had natural infections of J. pasqualei cysts in the liver and attached to the intestine. All P. siculus lizards and experimentally infected rodents and beetles were negative. DNA sequences obtained from J. pasqualei showed the highest nucleotide similarities with Versteria sp., Echinococcus sp., Raillietina sonini, Taenia polyacantha and D. caninum. Data herein provided show the inability of rodents to become infected by direct ingestion of gravid proglottids, suggesting a need for an invertebrate first intermediate host in the life cycle. Thus, more research study is advocated to better understand the biology of J. pasqualei such as its first intermediate host and its mechanism of transmission in reptiles and rodents
Спогад про красу вірності
No full textThe essay deals with Oles Honchar’s works interpreted in the light of the writer’s own memoirs. It analyzes Honchar’s views on literature in general and his own novels in particular. The researcher considers the issues of relations between reality and literary work, the features of literary process in conditions of ideological blockage, dependence of a talent on cultural traditions of the people and specificity of individual endowments.
Attention is paid to the functions of the main writer’s works and their place in Ukrainian literary process and ideological disputes of the time (“Modry Stone”, “Standard-bearers”, “Man and Weapons”, “For a Moment of Happiness”, “Tronka”, “Cathedral”, “Your star”, “Cyclone”, “Distant Fires”, “Reflections of a Writer”, “Things We Live For”). In particular, the essay offers an attempt to reveal the cause of political debate around the story “Modry Stone” and the novel “Cathedral”, in which the writer showed his search for new stylistic means. The author discusses the interests of Honchar as a writer and a critiс, his views on the poetics of creativity, the tasks of literature under conditions of dictatorial regimes, Ukrainian independence and international cooperation of creative personalities.У статті йдеться про творчість Олеся Гончара, осмислену крізь призму авторських спогадів. Аналізуються погляди письменника на літературу загалом і власні твори зокрема. Зачеплено питання зв’язку художньої творчості з реальністю, з особливостями розвитку літературного процесу в умовах ідеологічної заблокованості, залежності таланту від культурних традицій народу та специфіки його індивідуального обдаровання
Remembering the Beauty of Fidelity
No full textУ статті йдеться про творчість Олеся Гончара, осмислену крізь призму авторських спогадів.
Аналізуються погляди письменника на літературу загалом і власні твори зокрема. Зачеплено
питання зв'язку художньої творчості з реальністю, з особливостями розвитку літературного
процесу в умовах ідеологічної заблокованості, залежності таланту від культурних традицій
народу та специфіки його індивідуального обдаровання.The essay deals with Oles Honchar’s works interpreted in the light of the writer’s own memoirs. It
analyzes Honchar’s views on literature in general and his own novels in particular. The researcher
considers the issues of relations between reality and literary work, the features of literary process in
conditions of ideological blockage, dependence of a talent on cultural traditions of the people and
specificity of individual endowments.
Attention is paid to the functions of the main writer’s works and their place in Ukrainian literary process
and ideological disputes of the time (“Modry Stone”, “Standard-bearers”, “Man and Weapons”, “For a
Moment of Happiness”, “Tronka”, “Cathedral”, “Your star”, “Cyclone”, “Distant Fires”, “Reflections of a
Writer”, “Things We Live For”). In particular, the essay offers an attempt to reveal the cause of political
debate around the story “Modry Stone” and the novel “Cathedral”, in which the writer showed his
search for new stylistic means. The author discusses the interests of Honchar as a writer and a critiс,
his views on the poetics of creativity, the tasks of literature under conditions of dictatorial regimes,
Ukrainian independence and international cooperation of creative personalities
Remembering the Beauty of Fidelity
No full textУ статті йдеться про творчість Олеся Гончара, осмислену крізь призму авторських спогадів.
Аналізуються погляди письменника на літературу загалом і власні твори зокрема. Зачеплено
питання зв'язку художньої творчості з реальністю, з особливостями розвитку літературного
процесу в умовах ідеологічної заблокованості, залежності таланту від культурних традицій
народу та специфіки його індивідуального обдаровання.The essay deals with Oles Honchar’s works interpreted in the light of the writer’s own memoirs. It
analyzes Honchar’s views on literature in general and his own novels in particular. The researcher
considers the issues of relations between reality and literary work, the features of literary process in
conditions of ideological blockage, dependence of a talent on cultural traditions of the people and
specificity of individual endowments.
Attention is paid to the functions of the main writer’s works and their place in Ukrainian literary process
and ideological disputes of the time (“Modry Stone”, “Standard-bearers”, “Man and Weapons”, “For a
Moment of Happiness”, “Tronka”, “Cathedral”, “Your star”, “Cyclone”, “Distant Fires”, “Reflections of a
Writer”, “Things We Live For”). In particular, the essay offers an attempt to reveal the cause of political
debate around the story “Modry Stone” and the novel “Cathedral”, in which the writer showed his
search for new stylistic means. The author discusses the interests of Honchar as a writer and a critiс,
his views on the poetics of creativity, the tasks of literature under conditions of dictatorial regimes,
Ukrainian independence and international cooperation of creative personalities
Remembering the Beauty of Fidelity
No full textУ статті йдеться про творчість Олеся Гончара, осмислену крізь призму авторських спогадів.
Аналізуються погляди письменника на літературу загалом і власні твори зокрема. Зачеплено
питання зв'язку художньої творчості з реальністю, з особливостями розвитку літературного
процесу в умовах ідеологічної заблокованості, залежності таланту від культурних традицій
народу та специфіки його індивідуального обдаровання.The essay deals with Oles Honchar’s works interpreted in the light of the writer’s own memoirs. It
analyzes Honchar’s views on literature in general and his own novels in particular. The researcher
considers the issues of relations between reality and literary work, the features of literary process in
conditions of ideological blockage, dependence of a talent on cultural traditions of the people and
specificity of individual endowments.
Attention is paid to the functions of the main writer’s works and their place in Ukrainian literary process
and ideological disputes of the time (“Modry Stone”, “Standard-bearers”, “Man and Weapons”, “For a
Moment of Happiness”, “Tronka”, “Cathedral”, “Your star”, “Cyclone”, “Distant Fires”, “Reflections of a
Writer”, “Things We Live For”). In particular, the essay offers an attempt to reveal the cause of political
debate around the story “Modry Stone” and the novel “Cathedral”, in which the writer showed his
search for new stylistic means. The author discusses the interests of Honchar as a writer and a critiс,
his views on the poetics of creativity, the tasks of literature under conditions of dictatorial regimes,
Ukrainian independence and international cooperation of creative personalities
Emerg Infect Dis
No full textWe detected Leishmania infantum infection in 45% of tigers and 5.3% of sand flies tested at a zoo in southern Europe in 2019. These infections in tigers and the abundance of Phlebotomus perniciosus sand flies represent a potential risk to other animals and humans living in or visiting the zoo
Candidate gene molecular markers as tools for analyzing genetic susceptibility to morbillivirus infection in stranded Cetaceans
No full textMorbilliviruses, such as Cetacean morbillivirus (CeMV) or Phocine distemper virus (PDV), represent a growing threat for marine mammals on both hemispheres. Because free-ranging animal populations strongly rely on natural resistance mechanisms, innate immunity-related genes and virus cell entry receptor genes may represent key factors involved in susceptibility to CeMV in Cetaceans. Using the next generation sequencing technology, we have sequenced 11 candidate genes in two model species, Stenella coeruleoalba and Phocoena phocoena. Suitable single nucleotide polymorphism markers of potential functional importance, located in genes coding for basigin (BSG, CD147), the signaling lymphocyte activating molecule (SLAMF1), the poliovirus-related receptor-4 (NECTIN4, PVRL4), toll-like receptors 3, 7, 8 (TLR3, TLR7, TLR8), natural resistance-associated macrophage protein (SLC11A1) and natural cytotoxicity triggering receptor 1 (NCR1), were identified in each model species, along with MHC-DQB haplotypes unique for each species. This set of molecular markers represents a potentially useful tool for studying host genetic variation and susceptibility to morbillivirus infection in Cetaceans as well as for studying functionally important genetic diversity of selected Cetacean populations
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