68,791 research outputs found
Rudolph Wittkower and Colin Rowe: continuità e frattura
No full textL'influenza dei metodi di Rudolph Wittkower su Colin row
MENDEZ COLIN, Manuel
No full textLetter from Mr. Manuel Méndez Colin to Gen. Alvaro Obregón congratulating him on his election victory. / Carta del Sr. Manuel Méndez Colin al Gral. Alvaro Obregón felicitándolo por su triunfo electoral
MENDEZ COLIN, Manuel
No full textLetter from Mr. Manuel Méndez Colin to Gen. Alvaro Obregón congratulating him on his election victory. / Carta del Sr. Manuel Méndez Colin al Gral. Alvaro Obregón felicitándolo por su triunfo electoral
Influences without footnotes: Sibyl Moholy-Nagy and Colin Rowe
No full textColin Rowe es considerado como uno de los pensadores más relevantes en el campo arquitectónico desde la década de los 60. Su complejo andamiaje teórico muestra a una persona capaz de reconciliar críticamente gran variedad de las corrientes y discursos de su época, de ahí la riqueza y complejidad de sus textos. En el caso de Collage City, su propuesta se enmarca en un periodo crítico en relación a las doctrinas urbanas resultantes del pensamiento adscrito a la arquitectura moderna. La publicación se deriva del trabajo realizado en el Urban Design Program, dirigido por Rowe, en la Universidad de Cornell a partir de 1965. La teoría perceptiva de la Gestalt, la crítica estructuralista de Claude Levi-Strauss, la utopía política de Judith Shklaar o el método antihistoricista de Karl Popper, se puede rastrear de manera más o menos directa entre sus fuentes. Recientemente, la publicación de the letter of Colin Rowe: !ve decades o correpondence (2016), editada por Daniel Naegele, nos permite adentrarnos en la intrahistoria más privada del profesor. Entre toda esta correspondencia infraestructural, una mención en particular abre un nuevo camino de interpretación de la obra. Matrix of Man (1968) un libro dedicado a la historia urbana, escrito por la historiadora Sibyl Moholy-Nagy, parece haber sido uno de los libros guía que Rowe consultaba durante el proceso editorial de Collage City. Sin embargo, no existe ninguna mención de esta publicación en la obra de Fred Koetter y Colin Rowe. Este artículo intentará analizar hasta qué punto el libro de Sibyl Moholy-Nagy pudo in!uir en Colin Rowe, proponiendo nuevas aristas de interpretación de Collage City.Postprint (published version
A proof of concept of a BioMEMS glucose biosensor using microfabricated SU-8 films
No full textThe present project investigated and proved the concept of developing a novel
BioMEMS glucose micro-biosensor using a simple one-step microfabrication process of
the widely used SU-8 polymer. More specifically, the study focused on the investigation
of the suitability of the SU-8 polymer as a matrix for enzyme immobilisation that is
carried out during the microfabrication process. A comparative study between
commercially available SU-8 and “customised” SU-8 solutions showed that the
optimum concentration of photo-initiator for stress reduction can be achieved easier
with “customised” SU-8 solutions. The most appropriate type of microstructure for the
SU-8 matrix and the corresponding required microfabrication process were defined and
encapsulation of the enzyme GOx in the SU-8 solution was accomplished. A detailed
experimental investigation of the immobilised enzyme’s activity inside the SU-8 matrix,
was carried out using amperometric detection of hydrogen peroxide in a 3-electrode setup.
SU-8 films were immersed in a buffer solution and the platinum working electrode
was brought in close contact with the film. Films without enzyme showed negligible
variation in current upon the addition of glucose, as opposed to films with encapsulated
enzyme which showed a very clear increase in current. Experiments using films of
increased thickness or enzyme concentration, showed a higher response, thus proving
that the enzyme remained active not only on the film’s surface, but inside the matrix as
well. In the fluorescence spectroscopy experiments, the utilisation of the tris (4,7-
diphenyl-1,10-phenanthroline) ruthenium(II) dichloride oxygen indicator, which was
also captured in the polymer matrix during the microfabrication process, was proven to
be very sensitive to glucose concentration changes during the glucose oxidation and
there was no photo-bleaching.
The experimental investigations proved that the proposed concept of using SU-8
matrices for the immobilisation of biomolecules, is a valid proposal for the construction
of a BioMEMS glucose biosensor. An important outcome was the successful
immobilisation of glucose oxidase in SU-8 microfabricated structures. The enzyme still
showed activity despite the “hostile” conditions during microfabrication The proof of
principle of enzyme immobilisation in SU-8 films opens up new possibilities for
combining BioMEMS with biosensors and organic electronics
Investigating the development of midbrain dopaminergic neurons using mouse embryonic stem cell reporter lines
No full textEmbryonic stem (ES) cells possess the capability to self-renew indefinitely and are capable of generating any cell of the three primary germ layers, making them an attractive source of material to investigate both basic physiological properties and neurodegenerative processes. Although ES cells can be directed into specific cell lineages, the differentiation of ES cells results in heterogenous cultures. To date, there are few differentiation protocols that produce homogenous populations of any desired cell type. Many methods have been used in an effort to obtain homogenous populations of cells; from forced expression of genes involved in developmental pathways, to FACS isolation of cells expressing markers of interest. There has been a considerable focus on generating homogenous populations of midbrain dopaminergic progenitors (or neurons) for Parkinson's disease which involves the degeneration of a specific population of midbrain dopaminergic neurons. In this thesis, I investigate the development of mouse embryonic stem cells into midbrain dopaminergic neurons using reporter cell lines. In the first experimental chapter, I investigate the expression of Lmx1a and Msx1; two key transcription factors implicated in dopaminergic neuronal development. I also examine the impact of the BMP, Shh and Wnt signalling pathways on dopaminergic neural differentiation. Activation of the BMP and Wnt pathways resulted in inhibition of neural induction and the expression of both Lmx1a and Msx1. In contrast, antagonising these signalling pathways increased the yield of tyrosine hydroxylase (TH) expressing neurons. Activating or inhibiting the Shh pathway did not affect Lmx1a, Msx1 or TH expression. These experiments show that early Lmx1a expression is not indicative of the number of dopaminergic neurons produced. Furthermore, many of the TH positive neurons derived from monolayer cultures were not of midbrain origin. In the following experimental chapter, I used immunocytochemistry and qPCR to characterise the population of cells expressing Lmx1a. The downstream targets of Lmx1a, Msx1 and Wnt1, and midbrain dopaminergic neuron markers, Lmx1b and En1, were significantly upregulated in Lmx1a positive cells. The Lmx1a positive fraction was enriched with neural progenitors, and give rise to highly neural cultures. However, the majority of neurons in the terminally differentiated cultures derived from Lmx1a positive cells were GABAergic. Immunocytochemistry identified these cells as forebrain GABAergic neurons with upper-layer identity. Furthermore, the isolated Lmx1a positive cells were not responsive to patterning cues, indicating that they were already committed towards a GABAergic neuron fate. To show that these Lmx1a+ progenitors could generate dopaminergic neurons I used an alternative differentiation paradigm, the PA6 co-culture method. Expression of Lmx1a in PA6 co-cultures was different from monolayer cultures; the percentage of Lmx1a positive cells increased throughout the differentiation period. In addition, PA6 co-culture derived TH positive cells were found to co-express Lmx1a, an occurrence that was uncommon in monolayer cultures. The ionotropic glutamate receptors on neurons derived on adherent monolayer and PA6 co-cultures were functionally characterised in the final experimental chapter. Previously, antagonism of ionotropic glutamate receptors has been reported to improve behavioural assay scores in Parkinsonian animal models (Johnson et al., 2009). Terminally differentiated monolayer cultures and PA6 co-cultures responded differently to stimulation with glutamate, AMPA kainate and NMDA. The ionotropic glutamate receptors of midbrain dopaminergic and GABAergic neurons derived from both culture systems were further investigated. An initial characterisation indicates distinct differences between the glutamate receptor populations in monolayer and PA6 co-cultures. It appears that monolayer differentiation generates AMPA expressing midbrain dopaminergic neurons in comparison to the NMDA receptors evident following PA6 differentiation. Interestingly, these differences in receptor expression appear restricted by culture method, rather than neuronal subtype, i.e. monolayer neurons expressed AMPA receptors, regardless of whether they were TH+ or GAD67+. Similarly both TH+ and GAD67+ neurons appeared to express NMDA receptors following PA6 differentiation. At present the significance of these findings is unknown. In addition, the effect of Wnt5a on cell responses to glutamate agonists was examined. Wnt5a was able to potentiate cell responses to sub-maximal concentrations of certain glutamate agonists depending on the differentiation paradigm performed
Compte rendu de : Christopher J. Tozzi, Nationalizing France’s Army : foreign, Black, and Jewish troops in the French military, 1715-1831, Charlottesville/London, University of Virginia Press, 2016
No full textInternational audienceDistribution électronique Cairn.info pour Armand Colin. © Armand Colin. Tous droits réservés pour tous pays. La reproduction ou représentation de cet article, notamment par photocopie, n'est autorisée que dans les limites des conditions générales d'utilisation du site ou, le cas échéant, des conditions générales de la licence souscrite par votre établissement. Toute autre reproduction ou représentation, en tout ou partie, sous quelque forme et de quelque manière que ce soit, est interdite sauf accord préalable et écrit de l'éditeur, en dehors des cas prévus par la législation en vigueur en France. Il est précisé que son stockage dans une base de données est également interdit
Influencias sin notas al pie: Sibyl Moholynagy & Colin Rowe
No full textColin Rowe es considerado como uno de los pensadores más relevantes en el campo arquitectónico desde la década de los 60. Su complejo andamiaje teórico muestra a una persona capaz de reconciliar críticamente gran variedad de las corrientes y discursos de su época, de ahí la riqueza y complejidad de sus textos. En el caso de Collage City, su propuesta se enmarca en un periodo crítico en relación a las doctrinas urbanas resultantes del pensamiento adscrito a la arquitectura moderna. La publicación se deriva del trabajo realizado en el Urban Design Program, dirigido por Rowe, en la Universidad de Cornell a partir de 1965. La teoría perceptiva de la Gestalt, la crítica estructuralista de Claude Levi-Strauss, la utopía política de Judith Shklaar o el método anti-historicista de Karl Popper, se puede rastrear de manera más o menos directa entre sus fuentes. Recientemente, la publicación de the letter of Colin Rowe: five decades o correpondence (2016), editada por Daniel Naegele, nos permite adentrarnos en la intrahistoria más privada del profesor. Entre toda esta correspondencia infraestructural, una mención en particular abre un nuevo camino de interpretación de la obra. Matrix of Man (1968) un libro dedicado a la historia urbana, escrito por la historiadora Sibyl Moholy-Nagy, parece haber sido uno de los libros guía que Rowe consultaba durante el proceso editorial de Collage City. Sin embargo, no existe ninguna mención de esta publicación en la obra de Fred Koetter y Colin Rowe. Este artículo intentará analizar hasta qué punto el libro de Sibyl Moholy-Nagy pudo influir en Colin Rowe, proponiendo nuevas aristas de interpretación de Collage City
Nuevos Alfabetismos: su pràctica cotidiana y el aprendizaje en el aula
No full textThe first edition of this work was reviewing the new literacies, new types of knowledge and classroom activities in the context of the massive increase in digital information technologies and communication. The enormous development of this field allowed the authors, at the time in which the first edition sold out, carry out a second edition, completely renovated and updated. This timely new version almost simultaneously published in English and Spanish, explains and comments a whole new set of activities like blogging, fanfiction, mobile and wireless communications and practices remix fan of sonic and visual themes.
Thus, in this second edition revised and updated, the book examines:
• General practice and social networks related to contemporary phenomena such as Flickr and Wikipedia.
• Blogs, podcasting and mobile and wireless communication.
• Writing activities in online communities and manga-anime fanfiction.
• Production of Anime, music and video and "memes" multimodal.
Colin Lankshear and Michele Knobel discusses how youth have embraced digital technologies and new forms of mobile communications, and integrated into their daily lives. They argue that educational institutions ignore at their own peril many of these tendencies and expose how you can effectively integrate wireless mobility school pedagogies, paying due attention to new literacies in teaching and learning. These new resources help connect the school world with the interests and everyday activities girls and boys outside the classroom.
This book is essential reading for university students and teachers
Contextualizando a Colin Rowe con Jane Jacobs: complejidad, contexto y dependencia en la arquitectura
No full textMBArch - Màster Universitari en Estudis Avançats en Arquitectura-Barcelona: Teoria, Història i CulturaEn 1947, Colin Rowe, por medio del ensayo Las matemáticas de la vivienda ideal, probaría de manera irrefutable, por medio de una atrevida comparación entre la Villa Stein en Garches de Le Corbusier y La Malcontenta en el Veneto de Andrea Palladio, que la arquitectura del movimiento moderno “no era revolucionaria y separada de la historia, como pretendía ser, sino evolucionaria y conectada con la historia”. Este ensayo se inspira en esa atrevida comparación para, por medio de una yuxtaposición de las principales obras sobre la ciudad del mismo Colin Rowe y de Jane Jacobs, activista y autora de Muerte y Vida de las Grandes Ciudades, acercar a los dos personajes, mostrar similitudes en su pensamiento y sugerir que la arquitectura (y el diseño urbano) no es ni debe ser una disciplina autónoma, y que es mas bien dependiente de las estructuras sociales, políticas y económicas dentro de las cuales opera. El ensayo aborda temas de complejidad, contexto y dependencia en la arquitectura.In 1947, Colin Rowe, in the essay The Mathematics of the Ideal Villa, would prove irrefutably, through a comparison between Villa Stein in Garches by Le Corbusier and La Malcontenta in the Veneto by Andrea Palladio, that the modern architecture movement “was not revolutionary, as it was supposed to be, but rather evolutionary and connected to history”. This essay is inspired on that comparison in order to, by means of a juxtaposition of the main texts on the city written by Colin Rowe himself and Jane Jacobs, activist and author of Death and Life of Great American Cities, bring these two characters closer together, show similarities in their thought and suggest that architecture (and urban design) is not and should not be considered an autonomous discipline, but is rather dependent on the social, political and economic structures within which it operates. The essay addresses issues of complexity, context and dependence in architecture.Objectius de Desenvolupament Sostenible::5 - Igualtat de Gèner
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