152,200 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
Unimodality of Betti numbers for Hamiltonian circle actions with index-increasing moment Maps
No full textThe unimodality conjecture posed by Tolman in [L. Jeffrey, T. Holm, Y. Karshon, E. Lerman and E. Meinrenken, Moment maps in various geometries, http://www.birs.ca/workshops/2005/05w5072/report05w5072.pdf] states that if (M,ω) is a 2n-dimensional smooth compact symplectic manifold equipped with a Hamiltonian circle action with only isolated fixed points, then the sequence of Betti numbers {b0(M),b2(M),...,b2n(M)} is unimodal, i.e. bi(M) ≤ bi+2(M) for every i < n. Recently, the author and Kim [Y. Cho and M. Kim, Unimodality of the Betti numbers for Hamiltonian circle action with isolated fixed points, Math. Res. Lett. 21(4) (2014) 691-696] proved that the unimodality holds in eight-dimensional case by using equivariant cohomology theory. In this paper, we generalize the idea in [Y. Cho and M. Kim, Unimodality of the Betti numbers for Hamiltonian circle action with isolated fixed points, Math. Res. Lett. 21(4) (2014) 691-696] to an arbitrary dimensional case. We prove the conjecture in arbitrary dimension under the assumption that the moment map H : M → R is index-increasing, which means that ind(p) < ind(q) implies H(p) < H(q) for every pair of critical points p and q of H, where ind(p) is the Morse index of p with respect to H. © World Scientific Publishing Company1111sciescopu
TG, T-cho, H-cho, and cortisol levels in the serum.
No full text<p>CMS group, chronic mild stress group; C group, control group; TG, triglyceride; T-cho, total cholesterol; H-cho, HDL-cholesterol; SD, standard deviation.</p><p>TG, T-cho, H-cho, and cortisol levels in the serum.</p
Site-selective enzymatic C‒H amidation for synthesis of diverse lactams
No full textPlease note that this work has been retracted by the authors.After publication of the Report “Site-selective enzymatic C‒H amidation for synthesis of diverse lactams” in Science (1, also linked in metadata), efforts to reproduce the work showed that the enzymes do not catalyze the reactions with the activities and selectivities claimed. Careful examination of the first author’s lab notebook then revealed missing contemporaneous entries and raw data for key experiments. The authors therefore have retracted the article from Science and are now retracting the preprint from ChemRxiv. The original paper can be accessed by selecting “Version 1” of the preprint below, or by accessing https://doi.org/10.26434/chemrxiv.7711418.v1. Inha Cho, Zhi-Jun Jia, Frances H. Arnold*Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.*Corresponding author. Email: [email protected]. I. Cho, Z.-J. Jia, F. H. Arnold, Science 364, 575 (2019). DOI: 10.1126/science.aaw9068*******************************************************************************************Directed evolution of cytochrome P450 enzymes fine-tunes site selectivity of new-to-nature C‒H amidation for modular, sustainable and scalable preparation of enantio-enriched β-, γ- and δ-lactams.</p
Acoustic Source Power Control and Global Noise Reduction by Arrangement of Absorptive Materials in Acoustically Small Enclosures
No full textThe possibility of global noise reduction by the sound power control is discussed. It is necessary to investigate the relation between the global sound energy in the field and the total sound power radiated by sources. In the previous work [1,2], the authors presented a useful design method to change boundary condition that reduces noise in acoustically small enclosures. Changing boundary condition is related to not only its geometrical shape but also acoustical treatment on walls; for example, attaching of impedance patches (ex: absorptive material). In many practical situations, we often meet a situation to change acoustical treatment on walls. A paper related to what we envisaged was presented at Inter-noise 2003 (S.-H. Cho, Y.-H. Kim, and K. Grosh, “The effect of impedance patch position on the sound field of an acoustically small cavity,” in Proc. Inter-noise 2003, N236). In this paper, the relation between acoustic source power control and global noise reduction are discussed. The possibility of total acoustic potential energy, which is global noise, reduction by acoustic source power control is examined for acoustically small cavity. Using acoustic energy balance equation, the relation between global noise control performance and absorptive material arrangement is deduced. Simulation is performed to investigate the theoretical possibility and the power control trend in terms of total acoustic potential energy reduction with respect to the frequency range of interest
Gonioctena (Asiphytodecta) mausonensis Cho & Borowiec 2016
No full textGonioctena (Asiphytodecta) mausonensis Cho & Borowiec, 2016 (Figs 4, 9, 25–26, 29) Gonioctena (Asiphytodecta) flavoplagiata (partim): YANG et al. (2014): 384 (taxonomy, misidentification); YANG et al. (2015): 54 (catalogue, misidentification). Gonioctena (Asiphytodecta) mausonensis Cho & Borowiec, 2016: 169 (original description). Type locality. Vietnam, Lang Son Province, Loc Binh, Mt. Mau Son. Type material examined. HOLOTYPE: ♂, ‘ Tonkin, Montes Mauson,April, Mai 2-3000’, H. Fruhstorfer // 24 // Jacoby Coll.1909-28a. // flavoplagiata Jac. // HOLOTYPUS Gonioctena (Asiphy.) mausonensis sp. n. Cho & Borowiec 2014’ (BMNH). PARATYPE: 1 ♂, ‘ Tonkin, Montes Mauson,April, Mai 2-3000′, H. Fruhstorfer // PARATYPUS Gonioctena (Asiphy.) mausonensis sp. n. Cho & Borowiec 2014’ (LMCM). Description. See CHO & BOROWIEC (2016). Differential diagnosis. Gonioctena (A.) mausonensis can be easily distinguished from G. (A.) eburoides, G. (A.) flavoplagiata, G. (A.) jindrai sp. nov. and G. (A.) truncaticornis sp. nov. by its reddish-brown venter and subdiamond-shaped apical process of aedeagus (Fig. 26). Distribution. Vietnam: Lang Son (Fig. 29). Remarks. The male genitalia of Gonioctena (A.) flavoplagiata illustrated by YANG et al. (2014) without any doubts refer to G. (A.) mausonensis. The correct drawings of male genitalia of G. (A.) flavoplagiata were provided by CHO & BOROWIEC (2016).Published as part of Cho, Hee-Wook & Borowiec, Lech, 2016, Revision of theGonioctena flavoplagiata species-group (Coleoptera: Chrysomelidae: Chrysomelinae), with descriptions of two new species from China and Laos, pp. 755-768 in Acta Entomologica Musei Nationalis Pragae 56 (2) on page 763, DOI: 10.5281/zenodo.531035
Gonioctena (Brachyphytodecta) mauroi Cho & Borowiec 2016
No full textGonioctena (Brachyphytodecta) mauroi Cho & Borowiec, 2016 (Figs 11, 13) Gonioctena (Brachyphytodecta) mauroi Cho & Borowiec, 2016a: 176 (original description). Type locality. ‘ Vietnam, Lang Son Province, Loc Binh, Mt. Mau Son’. Type material examined. HOLOTYPE: ♂, ‘Tonkin, Montes Mauson, April, Mai 2-3000’, H. Fruhstorfer // HOLOTYPUS Gonioctena (Brachy.) mauroi sp. n. Cho & Borowiec 2015’ (LMCM). Description. See CHO & BOROWIEC (2016a). Differential diagnosis. Gonioctena (B.) mauroi is very similar to G. (B.) longshengensis sp. nov. and G. (B.) medvedevi in body shape, coloration and setose aedeagus. From these two species, G. (B.) mauroi can be distinguished only by aedeagus subparallel-sided with apical process widest in middle and slightly narrower than median lobe (subparallel-sided with apical process broad, widest in apical 1/3 and subequal to median lobe in width in G. (B.) longshengensis sp. nov. and moderately narrowed with apical process moderately widened to lateral tooth-like projections near apex in G. (B.) medvedevi). Gonioctena (B.) kuatunensis sp. nov. differs in semicircular apex of aedeagus (triangular or subtriangular in other species). Distribution. Vietnam: Lang Son Province (Fig. 13).Published as part of Cho, Hee-Wook, 2017, Two new species of the Gonioctena mauroi species-group from China (Coleoptera: Chrysomelidae: Chrysomelinae), pp. 173-181 in Acta Entomologica Musei Nationalis Pragae 57 (1) on page 180, DOI: 10.1515/aemnp-2017-0066, http://zenodo.org/record/531615
Dataset for Lasting effects of discontinuous shear thickening in cornstarch suspensions upon flow cessation
No full textThis dataset supports the publication: 'Lasting effects of discontinuous shear thickening in cornstarch suspensions upon flow cessation' by Jae Hyung Cho, Andrew H. Griese, Ivo Peters and Irmgard Bischofberger, Physical Review Fluids (2022) The data is in .xlsx format accessible in excel.
The file contains data to reproduce the following figures:
Fig 1. Flow curves
Fig 3. Temporal change in stresses and shear rate
Fig 4. Characteristic times
Fig 5. Transition times, scale parameter T, stress at transition, and residual stress
Fig 7. Transition mean normal stress
The data in the file is organized by figure, with a separate sheet for each figure containing the data to reproduce the respective figure</span
The ALMA-PILS survey: First detection of the unsaturated 3-carbon molecules Propenal (CHCHO) and Propylene (CH) towards IRAS 162932422 B
No full textAccepted for publication in A&A. 24 pages, 17 figuresInternational audienceComplex organic molecules with three carbon atoms are found in the earliest stages of star formation. In particular, propenal (CHCHO) is a species of interest due to its implication in the formation of more complex species and even biotic molecules. This study aims to search for the presence of CHCHO and other three-carbon species such as propylene (CH) in the hot corino region of the low-mass protostellar binary IRAS 16293--2422 to understand their formation pathways. We use ALMA observations in Band 6 and 7 from various surveys to search for the presence of CH and CHCHO towards the protostar IRAS 16293--2422 B (IRAS 16293B). We report the detection of both CH and CHCHO towards IRAS 16293B, however, no unblended lines were found towards the other component of the binary system, IRAS 16293A. We derive column density upper limits for CH, HCCCHO, n-CHOH, i-CHOH, CO, and cis-HC(O)CHO towards IRAS 16293B. We then use a three-phase chemical model to simulate the formation of these species in a typical prestellar environment followed by its hydrodynamical collapse until the birth of the central protostar. Different formation paths, such as successive hydrogenation and radical-radical additions on grain surfaces, are tested and compared to the observational results. The simulations reproduce the abundances within one order of magnitude from those observed towards IRAS 16293B, with the best agreement found for a rate of cm s for the gas-phase reaction C + O C + CO. Successive hydrogenations of C, HC(O)CHO, and CHOCHO on grain surfaces are a major and crucial formation route of complex organics molecules, whereas both successive hydrogenation pathways and radical-radical addition reactions contribute to the formation of CHCHO
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